YLR113W / HOG1

Mitogen-activated protein kinase involved in osmoregulation; controls global reallocation of RNA Pol II in response to osmotic shock; acts via three independent osmosensors; mitophagy-specific regulator; mediates the recruitment and activation of RNA Pol II at Hot1p-dependent promoters; localization regulated by Ptp2p and Ptp3p; protein abundance increases in response to DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YLR113W / HOG1

Click on Significant Values for Screen Details ID:SGTC_36|Compound:0335-0881|FD-Score:3.92|P-value:1.39E-4 ID:SGTC_42|Compound:0133-0086|FD-Score:-3.79|P-value:1.42E-4 ID:SGTC_178|Compound:4092-0806|FD-Score:4.66|P-value:7.25E-6 ID:SGTC_184|Compound:1494-0393|FD-Score:-3.39|P-value:5.84E-4 ID:SGTC_207|Compound:4185-0010|FD-Score:7.33|P-value:3.68E-12 ID:SGTC_266|Compound:formosanin C|FD-Score:5.97|P-value:1.29E-8 ID:SGTC_392|Compound:N-ethylmaleimide|FD-Score:3.36|P-value:9.34E-4 ID:SGTC_412|Compound:gemfibrozil|FD-Score:-3.28|P-value:8.19E-4 ID:SGTC_418|Compound:lividomycin a|FD-Score:-4.00|P-value:6.54E-5 ID:SGTC_690|Compound:glyoxal|FD-Score:10.70|P-value:6.92E-24 ID:SGTC_909|Compound:3448-9333|FD-Score:3.76|P-value:2.48E-4 ID:SGTC_1046|Compound:2001-0062|FD-Score:3.49|P-value:6.21E-4 ID:SGTC_1066|Compound:ketoconazole|FD-Score:-3.48|P-value:4.24E-4 ID:SGTC_1072|Compound:oxiconazole|FD-Score:4.01|P-value:1.01E-4 ID:SGTC_1081|Compound:sertraline|FD-Score:-4.32|P-value:1.83E-5 ID:SGTC_1131|Compound:3454-3128|FD-Score:4.33|P-value:2.84E-5 ID:SGTC_1132|Compound:3456-3614|FD-Score:-4.60|P-value:5.79E-6 ID:SGTC_1161|Compound:k007-0610|FD-Score:5.44|P-value:2.02E-7 ID:SGTC_1162|Compound:k048-0130|FD-Score:-4.00|P-value:6.50E-5 ID:SGTC_1317|Compound:1273-0028|FD-Score:3.49|P-value:6.05E-4 ID:SGTC_1340|Compound:1486-1359|FD-Score:-3.45|P-value:4.69E-4 ID:SGTC_1452|Compound:4194-0072|FD-Score:3.51|P-value:5.85E-4 ID:SGTC_1705|Compound:st027868|FD-Score:-3.38|P-value:6.00E-4 ID:SGTC_2421|Compound:5690543|FD-Score:-3.97|P-value:7.26E-5 ID:SGTC_2430|Compound:5571206|FD-Score:-3.46|P-value:4.63E-4 ID:SGTC_2463|Compound:5473429|FD-Score:-3.49|P-value:4.09E-4 ID:SGTC_2474|Compound:5321570|FD-Score:-3.45|P-value:4.70E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:3.61|P-value:4.13E-4 ID:SGTC_2716|Compound:st080858|FD-Score:-3.34|P-value:6.77E-4 ID:SGTC_2843|Compound:9000894|FD-Score:-3.47|P-value:4.40E-4 ID:SGTC_2979|Compound:9012552|FD-Score:3.44|P-value:7.15E-4 ID:SGTC_3202|Compound:9113761|FD-Score:-3.72|P-value:1.86E-4 ID:SGTC_3205|Compound:9113483|FD-Score:-3.47|P-value:4.35E-4 ID:SGTC_3206|Compound:9114350|FD-Score:5.01|P-value:1.55E-6 ID:SGTC_36|Compound:0335-0881|FD-Score:3.92|P-value:1.39E-4 ID:SGTC_42|Compound:0133-0086|FD-Score:-3.79|P-value:1.42E-4 ID:SGTC_178|Compound:4092-0806|FD-Score:4.66|P-value:7.25E-6 ID:SGTC_184|Compound:1494-0393|FD-Score:-3.39|P-value:5.84E-4 ID:SGTC_207|Compound:4185-0010|FD-Score:7.33|P-value:3.68E-12 ID:SGTC_266|Compound:formosanin C|FD-Score:5.97|P-value:1.29E-8 ID:SGTC_392|Compound:N-ethylmaleimide|FD-Score:3.36|P-value:9.34E-4 ID:SGTC_412|Compound:gemfibrozil|FD-Score:-3.28|P-value:8.19E-4 ID:SGTC_418|Compound:lividomycin a|FD-Score:-4.00|P-value:6.54E-5 ID:SGTC_690|Compound:glyoxal|FD-Score:10.70|P-value:6.92E-24 ID:SGTC_909|Compound:3448-9333|FD-Score:3.76|P-value:2.48E-4 ID:SGTC_1046|Compound:2001-0062|FD-Score:3.49|P-value:6.21E-4 ID:SGTC_1066|Compound:ketoconazole|FD-Score:-3.48|P-value:4.24E-4 ID:SGTC_1072|Compound:oxiconazole|FD-Score:4.01|P-value:1.01E-4 ID:SGTC_1081|Compound:sertraline|FD-Score:-4.32|P-value:1.83E-5 ID:SGTC_1131|Compound:3454-3128|FD-Score:4.33|P-value:2.84E-5 ID:SGTC_1132|Compound:3456-3614|FD-Score:-4.60|P-value:5.79E-6 ID:SGTC_1161|Compound:k007-0610|FD-Score:5.44|P-value:2.02E-7 ID:SGTC_1162|Compound:k048-0130|FD-Score:-4.00|P-value:6.50E-5 ID:SGTC_1317|Compound:1273-0028|FD-Score:3.49|P-value:6.05E-4 ID:SGTC_1340|Compound:1486-1359|FD-Score:-3.45|P-value:4.69E-4 ID:SGTC_1452|Compound:4194-0072|FD-Score:3.51|P-value:5.85E-4 ID:SGTC_1705|Compound:st027868|FD-Score:-3.38|P-value:6.00E-4 ID:SGTC_2421|Compound:5690543|FD-Score:-3.97|P-value:7.26E-5 ID:SGTC_2430|Compound:5571206|FD-Score:-3.46|P-value:4.63E-4 ID:SGTC_2463|Compound:5473429|FD-Score:-3.49|P-value:4.09E-4 ID:SGTC_2474|Compound:5321570|FD-Score:-3.45|P-value:4.70E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:3.61|P-value:4.13E-4 ID:SGTC_2716|Compound:st080858|FD-Score:-3.34|P-value:6.77E-4 ID:SGTC_2843|Compound:9000894|FD-Score:-3.47|P-value:4.40E-4 ID:SGTC_2979|Compound:9012552|FD-Score:3.44|P-value:7.15E-4 ID:SGTC_3202|Compound:9113761|FD-Score:-3.72|P-value:1.86E-4 ID:SGTC_3205|Compound:9113483|FD-Score:-3.47|P-value:4.35E-4 ID:SGTC_3206|Compound:9114350|FD-Score:5.01|P-value:1.55E-6

Top fitness defect scores for YLR113W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_690 glyoxal 10.70 20.3 µM 6.92E-24 16 6.04E-27
2 SGTC_207 4185-0010 7.33 4.5 µM 3.68E-12 endomembrane recycling 32 1.14E-13
3 SGTC_266 formosanin C 5.97 406.0 nM 1.29E-8 sphingolipid biosynthesis & PDR1 27 1.19E-9
4 SGTC_1161 k007-0610 5.44 110.0 µM 2.02E-7 ERAD & cell cycle 68 2.70E-8
5 SGTC_3206 9114350 5.01 49.5 µM 1.55E-6 6 2.72E-7
6 SGTC_178 4092-0806 4.66 87.5 µM 7.25E-6 ergosterol depletion effects on membrane 63 1.57E-6
7 SGTC_1131 3454-3128 4.33 32.3 µM 2.84E-5 27 7.36E-6
8 SGTC_1072 oxiconazole 4.01 14.9 nM 1.01E-4 ergosterol depletion effects on membrane 50 3.09E-5
9 SGTC_36 0335-0881 3.92 14.2 µM 1.39E-4 ubiquinone biosynthesis & proteasome 122 4.47E-5
10 SGTC_909 3448-9333 3.76 149.0 µM 2.48E-4 21 8.61E-5
11 SGTC_2582 agaric acid 3.61 100.0 µM 4.13E-4 redox potentiating 357 1.54E-4
12 SGTC_1452 4194-0072 3.51 190.0 µM 5.85E-4 25 2.28E-4
13 SGTC_1317 1273-0028 3.49 910.0 nM 6.05E-4 azole & statin 69 2.37E-4
14 SGTC_1046 2001-0062 3.49 216.0 µM 6.21E-4 29 2.44E-4
15 SGTC_2979 9012552 3.44 45.5 µM 7.15E-4 15 2.87E-4
16 SGTC_392 N-ethylmaleimide 3.36 8.3 µM 9.34E-4 RNA pol III & RNase P/MRP 30 3.88E-4
17 SGTC_1465 k213-0056 3.31 144.0 µM 0.00111 DNA intercalators 41 4.73E-4
18 SGTC_304 1310-0298 3.19 97.0 µM 0.00159 cell wall 98 7.10E-4
19 SGTC_1782 st039423 3.16 80.6 µM 0.00173 58 7.83E-4
20 SGTC_2599 2',3-dihydroxy-4,4',6'-trimethoxychalcone 3.11 100.0 µM 0.00203 67 9.35E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.255 8.62E-51 YKL213C DOA1 hom WD repeat protein required for ubiquitin-mediated protein degradation; forms a complex with Cdc48p; plays a role in controlling cellular ubiquitin concentration; also promotes efficient NHEJ in postdiauxic/stationary phase; protein increases in abundance and relocalizes from nucleus to nuclear periphery upon DNA replication stress
0.225 6.57E-40 YGR190C_d YGR190C_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene HIP1/YGR191W
0.221 1.85E-38 YNR031C SSK2 hom MAP kinase kinase kinase of the HOG1 mitogen-activated signaling pathway; interacts with Ssk1p, leading to autophosphorylation and activation of Ssk2p which phosphorylates Pbs2p; also mediates actin cytoskeleton recovery from osmotic stress
0.194 6.49E-30 YLR170C APS1 hom Small subunit of the clathrin-associated adaptor complex AP-1, which is involved in protein sorting at the trans-Golgi network; homolog of the sigma subunit of the mammalian clathrin AP-1 complex
0.193 1.54E-29 YJL128C PBS2 hom MAP kinase kinase of the HOG signaling pathway; activated under severe osmotic stress; mitophagy-specific regulator; plays a role in regulating Ty1 transposition
0.190 1.72E-28 YGR077C PEX8 hom Intraperoxisomal organizer of the peroxisomal import machinery; organizes the formation of the importomer complex, bridging the docking complex with the RING finger complex; tightly associated with the lumenal face of the peroxisomal membrane; essential for peroxisome biogenesis; binds PTS1-signal receptor Pex5p, and PTS2-signal receptor Pex7p
0.178 2.60E-25 YNL027W CRZ1 hom Transcription factor, activates transcription of stress response genes; nuclear localization is positively regulated by calcineurin-mediated dephosphorylation; rapidly localizes to the nucleus under blue light stress
0.176 7.19E-25 YBL024W NCL1 hom S-adenosyl-L-methionine-dependent tRNA: m5C-methyltransferase, methylates cytosine to m5C at several positions in tRNAs and intron-containing pre-tRNAs; increases proportion of tRNALeu(CAA) with m5C at wobble position in response to hydrogen peroxide, causing selective translation of mRNA from genes enriched in TTG codon; loss of NCL1 confers hypersensitivity to oxidative stress; similar to Nop2p and human proliferation associated nucleolar protein p120
0.173 4.54E-24 YOR364W_d YOR364W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOR365C
0.173 5.49E-24 YGR191W HIP1 het High-affinity histidine permease, also involved in the transport of manganese ions
0.155 1.46E-19 YNL307C MCK1 hom Protein serine/threonine/tyrosine (dual-specificity) kinase; involved in control of chromosome segregation and in regulating entry into meiosis; interacts with Clb2p and Mih1p; inhibits Clb2p-Cdk1 activity; related to mammalian glycogen synthase kinases of the GSK-3 family
0.155 1.79E-19 YHL019C APM2 hom Protein of unknown function, homologous to the medium chain of mammalian clathrin-associated protein complex; involved in vesicular transport
0.149 4.85E-18 YFL034W_p YFL034W_p hom Putative integral membrane protein that interacts with Rpp0p, which is a component of the ribosomal stalk
0.143 7.75E-17 YJL110C GZF3 hom GATA zinc finger protein; negatively regulates nitrogen catabolic gene expression by competing with Gat1p for GATA site binding; function requires a repressive carbon source; dimerizes with Dal80p and binds to Tor1p; GZF3 has a paralog, DAL80, that arose from the whole genome duplication
0.140 3.82E-16 YKL104C GFA1 het Glutamine-fructose-6-phosphate amidotransferase; catalyzes the formation of glucosamine-6-P and glutamate from fructose-6-P and glutamine in the first step of chitin biosynthesis; GFA1 has a paralogous region, comprising ORFs YMR084W-YMR085W, that arose from the whole genome duplication