YLR114C / AVL9

Conserved protein involved in exocytic transport from the Golgi; mutation is synthetically lethal with apl2 vps1 double mutation; member of a protein superfamily with orthologs in diverse organisms; relocalizes from bud neck to cytoplasm upon DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YLR114C / AVL9

Click on Significant Values for Screen Details ID:SGTC_326|Compound:k292-0812|FD-Score:-3.20|P-value:8.24E-4 ID:SGTC_386|Compound:1509-0011|FD-Score:-3.64|P-value:1.74E-4 ID:SGTC_669|Compound:0108-0021|FD-Score:7.89|P-value:8.86E-15 ID:SGTC_955|Compound:1216-0110|FD-Score:-3.71|P-value:1.36E-4 ID:SGTC_956|Compound:1185-0240|FD-Score:-3.73|P-value:1.24E-4 ID:SGTC_1056|Compound:3448-1962|FD-Score:3.45|P-value:4.19E-4 ID:SGTC_1300|Compound:1094-0077|FD-Score:3.19|P-value:9.98E-4 ID:SGTC_1642|Compound:st009582|FD-Score:-3.55|P-value:2.44E-4 ID:SGTC_1715|Compound:st032160|FD-Score:3.41|P-value:4.82E-4 ID:SGTC_1832|Compound:st054113|FD-Score:-3.42|P-value:3.83E-4 ID:SGTC_1834|Compound:st055981|FD-Score:-3.81|P-value:8.98E-5 ID:SGTC_1844|Compound:rapamycin|FD-Score:14.50|P-value:9.76E-46 ID:SGTC_1924|Compound:st058459|FD-Score:-3.92|P-value:5.98E-5 ID:SGTC_1925|Compound:st057770|FD-Score:4.92|P-value:9.20E-7 ID:SGTC_1933|Compound:st074711|FD-Score:5.52|P-value:4.24E-8 ID:SGTC_2001|Compound:4023502|FD-Score:5.04|P-value:5.05E-7 ID:SGTC_2011|Compound:4012611|FD-Score:3.30|P-value:7.02E-4 ID:SGTC_2275|Compound:7947463|FD-Score:-3.18|P-value:8.88E-4 ID:SGTC_2296|Compound:7971656|FD-Score:4.13|P-value:3.13E-5 ID:SGTC_2427|Compound:5545055|FD-Score:3.36|P-value:5.69E-4 ID:SGTC_2583|Compound:asiatic acid|FD-Score:-3.80|P-value:9.64E-5 ID:SGTC_2679|Compound:rapamycin|FD-Score:10.60|P-value:3.76E-25 ID:SGTC_2688|Compound:kanamycin b|FD-Score:4.71|P-value:2.47E-6 ID:SGTC_2699|Compound:st077704|FD-Score:4.09|P-value:3.69E-5 ID:SGTC_2706|Compound:st078239|FD-Score:3.64|P-value:2.09E-4 ID:SGTC_2708|Compound:st078559|FD-Score:-3.89|P-value:6.70E-5 ID:SGTC_3037|Compound:9091026|FD-Score:4.26|P-value:1.83E-5 ID:SGTC_3197|Compound:9113331|FD-Score:4.27|P-value:1.74E-5 ID:SGTC_3346|Compound:9158254|FD-Score:-3.31|P-value:5.66E-4 ID:SGTC_326|Compound:k292-0812|FD-Score:-3.20|P-value:8.24E-4 ID:SGTC_386|Compound:1509-0011|FD-Score:-3.64|P-value:1.74E-4 ID:SGTC_669|Compound:0108-0021|FD-Score:7.89|P-value:8.86E-15 ID:SGTC_955|Compound:1216-0110|FD-Score:-3.71|P-value:1.36E-4 ID:SGTC_956|Compound:1185-0240|FD-Score:-3.73|P-value:1.24E-4 ID:SGTC_1056|Compound:3448-1962|FD-Score:3.45|P-value:4.19E-4 ID:SGTC_1300|Compound:1094-0077|FD-Score:3.19|P-value:9.98E-4 ID:SGTC_1642|Compound:st009582|FD-Score:-3.55|P-value:2.44E-4 ID:SGTC_1715|Compound:st032160|FD-Score:3.41|P-value:4.82E-4 ID:SGTC_1832|Compound:st054113|FD-Score:-3.42|P-value:3.83E-4 ID:SGTC_1834|Compound:st055981|FD-Score:-3.81|P-value:8.98E-5 ID:SGTC_1844|Compound:rapamycin|FD-Score:14.50|P-value:9.76E-46 ID:SGTC_1924|Compound:st058459|FD-Score:-3.92|P-value:5.98E-5 ID:SGTC_1925|Compound:st057770|FD-Score:4.92|P-value:9.20E-7 ID:SGTC_1933|Compound:st074711|FD-Score:5.52|P-value:4.24E-8 ID:SGTC_2001|Compound:4023502|FD-Score:5.04|P-value:5.05E-7 ID:SGTC_2011|Compound:4012611|FD-Score:3.30|P-value:7.02E-4 ID:SGTC_2275|Compound:7947463|FD-Score:-3.18|P-value:8.88E-4 ID:SGTC_2296|Compound:7971656|FD-Score:4.13|P-value:3.13E-5 ID:SGTC_2427|Compound:5545055|FD-Score:3.36|P-value:5.69E-4 ID:SGTC_2583|Compound:asiatic acid|FD-Score:-3.80|P-value:9.64E-5 ID:SGTC_2679|Compound:rapamycin|FD-Score:10.60|P-value:3.76E-25 ID:SGTC_2688|Compound:kanamycin b|FD-Score:4.71|P-value:2.47E-6 ID:SGTC_2699|Compound:st077704|FD-Score:4.09|P-value:3.69E-5 ID:SGTC_2706|Compound:st078239|FD-Score:3.64|P-value:2.09E-4 ID:SGTC_2708|Compound:st078559|FD-Score:-3.89|P-value:6.70E-5 ID:SGTC_3037|Compound:9091026|FD-Score:4.26|P-value:1.83E-5 ID:SGTC_3197|Compound:9113331|FD-Score:4.27|P-value:1.74E-5 ID:SGTC_3346|Compound:9158254|FD-Score:-3.31|P-value:5.66E-4

Top fitness defect scores for YLR114C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1844 rapamycin 14.50 4.0 nM 9.76E-46 14 3.98E-48
2 SGTC_2679 rapamycin 10.60 1.0 nM 3.76E-25 21 1.87E-26
3 SGTC_669 0108-0021 7.89 26.2 µM 8.86E-15 fatty acid desaturase (OLE1) 10 1.57E-15
4 SGTC_1933 st074711 5.52 15.9 µM 4.24E-8 14 1.72E-8
5 SGTC_2001 4023502 5.04 57.7 µM 5.05E-7 mitochondrial processes 5 2.34E-7
6 SGTC_1925 st057770 4.92 10.1 µM 9.20E-7 TSC3-RPN4 8 4.41E-7
7 SGTC_2688 kanamycin b 4.71 96.7 µM 2.47E-6 calcium & mitochondrial duress 273 1.25E-6
8 SGTC_3197 9113331 4.27 49.5 µM 1.74E-5 26 9.77E-6
9 SGTC_3037 9091026 4.26 49.5 µM 1.83E-5 NEO1-PIK1 108 1.03E-5
10 SGTC_2296 7971656 4.13 126.5 µM 3.13E-5 39 1.82E-5
11 SGTC_2699 st077704 4.09 54.6 µM 3.69E-5 endomembrane recycling 46 2.16E-5
12 SGTC_2706 st078239 3.64 82.5 µM 2.09E-4 20 1.35E-4
13 SGTC_1056 3448-1962 3.45 115.0 µM 4.19E-4 amide catabolism 33 2.80E-4
14 SGTC_1715 st032160 3.41 10.2 µM 4.82E-4 63 3.25E-4
15 SGTC_2427 5545055 3.36 42.8 µM 5.69E-4 36 3.87E-4
16 SGTC_2011 4012611 3.30 177.0 µM 7.02E-4 mitochondrial processes 53 4.83E-4
17 SGTC_1300 1094-0077 3.19 113.0 µM 9.98E-4 35 7.01E-4
18 SGTC_3020 9089140 3.17 49.5 µM 0.00107 57 7.52E-4
19 SGTC_1283 0922-0125 3.11 16.4 µM 0.00130 54 9.28E-4
20 SGTC_2703 st077852 3.06 53.4 µM 0.00156 66 0.00112

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.474 7.35E-188 YKL203C TOR2 het PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that regulates growth in response to nutrients and TORC2, a complex that regulates cell-cycle dependent polarization of the actin cytoskeleton; involved in meiosis; TOR2 has a paralog, TOR1, that arose from the whole genome duplication
0.439 5.78E-158 YJR066W TOR1 hom PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that controls growth in response to nutrients by regulating translation, transcription, ribosome biogenesis, nutrient transport and autophagy; involved in meiosis; TOR1 has a paralog, TOR2, that arose from the whole genome duplication
0.387 1.36E-120 YML121W GTR1 hom Cytoplasmic GTP binding protein and negative regulator of the Ran/Tc4 GTPase cycle; component of GSE complex, which is required for sorting of Gap1p; involved in phosphate transport and telomeric silencing; similar to human RagA and RagB
0.380 9.31E-116 YKL110C KTI12 hom Protein that plays a role, with Elongator complex, in modification of wobble nucleosides in tRNA; involved in sensitivity to G1 arrest induced by zymocin; interacts with chromatin throughout the genome; also interacts with Cdc19p
0.374 3.41E-112 YLR384C IKI3 hom Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; maintains structural integrity of Elongator; homolog of human IKAP, mutations in which cause familial dysautonomia (FD)
0.359 1.12E-102 YNL119W NCS2 hom Protein required for thiolation of the uridine at the wobble position of Lys(UUU) and Glu(UUC) tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae
0.356 1.35E-100 YHR186C KOG1 het Subunit of TORC1, a rapamycin-sensitive complex involved in growth control that contains Tor1p or Tor2p, Lst8p and Tco89p; contains four HEAT repeats and seven WD-40 repeats; may act as a scaffold protein to couple TOR and its effectors
0.350 1.42E-96 YFL024C EPL1 het Subunit of NuA4, an essential histone H4/H2A acetyltransferase complex; conserved region at N-terminus is essential for interaction with the NPC (nucleosome core particle); required for autophagy; homologous to Drosophila Enhancer of Polycomb
0.340 2.21E-91 YHR187W IKI1 hom Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; iki1 mutations confer resistance to the K. lactis toxin zymocin
0.311 5.56E-76 YGL211W NCS6 hom Protein required for thiolation of the uridine at the wobble position of Gln, Lys, and Glu tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae
0.309 2.90E-75 YGR163W GTR2 hom Putative GTP binding protein that negatively regulates Ran/Tc4 GTPase cycle; activates transcription; subunit of EGO and GSE complexes; required for sorting of Gap1p; localizes to cytoplasm and to chromatin; homolog of human RagC and RagD
0.283 7.71E-63 YOR184W SER1 hom 3-phosphoserine aminotransferase; catalyzes the formation of phosphoserine from 3-phosphohydroxypyruvate, required for serine and glycine biosynthesis; regulated by the general control of amino acid biosynthesis mediated by Gcn4p; protein abundance increases in response to DNA replication stress
0.282 3.41E-62 YHR111W UBA4 hom Protein that activates Urm1p before its conjugation to proteins (urmylation); also acts in thiolation of the wobble base of cytoplasmic tRNAs by adenylating and then thiolating Urm1p; receives sulfur from Tum1p
0.281 4.86E-62 YDR023W SES1 het Cytosolic seryl-tRNA synthetase, class II aminoacyl-tRNA synthetase that aminoacylates tRNA(Ser), displays tRNA-dependent amino acid recognition which enhances discrimination of the serine substrate, interacts with peroxin Pex21p
0.281 1.44E-61 YBR077C SLM4 hom Component of the EGO complex, which is involved in the regulation of microautophagy, and of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; gene exhibits synthetic genetic interaction with MSS4