YLR117C / CLF1

Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; homolog of Drosophila crooked neck protein; interacts with U1 snRNP proteins

Zygosity: Heterozygous strain
fixedexpanded
Profile for YLR117C / CLF1

Click on Significant Values for Screen Details ID:SGTC_141|Compound:0443-0274|FD-Score:-9.81|P-value:1.86E-30 ID:SGTC_164|Compound:4100-3994|FD-Score:2.76|P-value:6.51E-4 ID:SGTC_169|Compound:k015-0017|FD-Score:3.44|P-value:3.00E-5 ID:SGTC_276|Compound:colchicine|FD-Score:3.41|P-value:3.41E-5 ID:SGTC_277|Compound:nystatin|FD-Score:2.89|P-value:3.80E-4 ID:SGTC_292|Compound:k038-0015|FD-Score:-2.80|P-value:5.80E-4 ID:SGTC_353|Compound:1988-1175|FD-Score:-4.11|P-value:9.19E-7 ID:SGTC_401|Compound:neomycin|FD-Score:-2.88|P-value:4.24E-4 ID:SGTC_416|Compound:estradiol valerate|FD-Score:3.29|P-value:6.33E-5 ID:SGTC_443|Compound:clozapine|FD-Score:2.74|P-value:6.93E-4 ID:SGTC_473|Compound:2-arachidonoylglycerol|FD-Score:-2.89|P-value:3.96E-4 ID:SGTC_545|Compound:4218-0007|FD-Score:-2.68|P-value:9.56E-4 ID:SGTC_567|Compound:1080-0542|FD-Score:2.68|P-value:8.71E-4 ID:SGTC_595|Compound:3909-8003|FD-Score:3.07|P-value:1.73E-4 ID:SGTC_682|Compound:0kpi-0099|FD-Score:-2.78|P-value:6.26E-4 ID:SGTC_859|Compound:0439-0102|FD-Score:3.20|P-value:9.56E-5 ID:SGTC_882|Compound:0843-0131|FD-Score:3.63|P-value:1.14E-5 ID:SGTC_1033|Compound:k072-0102|FD-Score:3.07|P-value:1.71E-4 ID:SGTC_1035|Compound:k081-0041|FD-Score:3.51|P-value:2.17E-5 ID:SGTC_1036|Compound:k216-0215|FD-Score:-5.37|P-value:2.15E-10 ID:SGTC_1038|Compound:k292-0204|FD-Score:-4.50|P-value:8.79E-8 ID:SGTC_1082|Compound:camptothecin|FD-Score:-3.84|P-value:4.16E-6 ID:SGTC_1125|Compound:tnp00248|FD-Score:2.68|P-value:8.96E-4 ID:SGTC_1205|Compound:4161-2785|FD-Score:2.70|P-value:8.25E-4 ID:SGTC_1402|Compound:3909-7673|FD-Score:3.69|P-value:8.40E-6 ID:SGTC_1514|Compound:1189-1591|FD-Score:2.72|P-value:7.59E-4 ID:SGTC_1674|Compound:st016616|FD-Score:-3.46|P-value:2.96E-5 ID:SGTC_1682|Compound:st012968|FD-Score:-3.08|P-value:1.74E-4 ID:SGTC_1772|Compound:st048439|FD-Score:2.81|P-value:5.28E-4 ID:SGTC_1851|Compound:st056236|FD-Score:3.45|P-value:2.89E-5 ID:SGTC_1854|Compound:st056233|FD-Score:2.94|P-value:3.05E-4 ID:SGTC_1855|Compound:st056195|FD-Score:4.32|P-value:2.32E-7 ID:SGTC_1858|Compound:4-chloro-8-(diethylamino)pyrano[3,2-c]chromene-2,5-dione|FD-Score:3.46|P-value:2.71E-5 ID:SGTC_1861|Compound:st057647|FD-Score:4.65|P-value:2.94E-8 ID:SGTC_2087|Compound:5276107|FD-Score:-3.40|P-value:3.88E-5 ID:SGTC_2091|Compound:4-phenylthiadiazole|FD-Score:-2.90|P-value:3.82E-4 ID:SGTC_2120|Compound:5538279|FD-Score:-2.87|P-value:4.26E-4 ID:SGTC_2187|Compound:6125751|FD-Score:2.72|P-value:7.61E-4 ID:SGTC_2323|Compound:6148992|FD-Score:-3.27|P-value:7.47E-5 ID:SGTC_2461|Compound:5755302|FD-Score:2.65|P-value:9.89E-4 ID:SGTC_2464|Compound:5717415|FD-Score:-3.16|P-value:1.23E-4 ID:SGTC_2484|Compound:5809818|FD-Score:-2.82|P-value:5.35E-4 ID:SGTC_2564|Compound:betulinic acid|FD-Score:-3.57|P-value:1.74E-5 ID:SGTC_2598|Compound:perillyl alcohol|FD-Score:2.72|P-value:7.39E-4 ID:SGTC_2794|Compound:7785382|FD-Score:8.62|P-value:4.92E-24 ID:SGTC_2809|Compound:7964133|FD-Score:3.06|P-value:1.81E-4 ID:SGTC_2811|Compound:7967822|FD-Score:-2.67|P-value:9.63E-4 ID:SGTC_141|Compound:0443-0274|FD-Score:-9.81|P-value:1.86E-30 ID:SGTC_164|Compound:4100-3994|FD-Score:2.76|P-value:6.51E-4 ID:SGTC_169|Compound:k015-0017|FD-Score:3.44|P-value:3.00E-5 ID:SGTC_276|Compound:colchicine|FD-Score:3.41|P-value:3.41E-5 ID:SGTC_277|Compound:nystatin|FD-Score:2.89|P-value:3.80E-4 ID:SGTC_292|Compound:k038-0015|FD-Score:-2.80|P-value:5.80E-4 ID:SGTC_353|Compound:1988-1175|FD-Score:-4.11|P-value:9.19E-7 ID:SGTC_401|Compound:neomycin|FD-Score:-2.88|P-value:4.24E-4 ID:SGTC_416|Compound:estradiol valerate|FD-Score:3.29|P-value:6.33E-5 ID:SGTC_443|Compound:clozapine|FD-Score:2.74|P-value:6.93E-4 ID:SGTC_473|Compound:2-arachidonoylglycerol|FD-Score:-2.89|P-value:3.96E-4 ID:SGTC_545|Compound:4218-0007|FD-Score:-2.68|P-value:9.56E-4 ID:SGTC_567|Compound:1080-0542|FD-Score:2.68|P-value:8.71E-4 ID:SGTC_595|Compound:3909-8003|FD-Score:3.07|P-value:1.73E-4 ID:SGTC_682|Compound:0kpi-0099|FD-Score:-2.78|P-value:6.26E-4 ID:SGTC_859|Compound:0439-0102|FD-Score:3.20|P-value:9.56E-5 ID:SGTC_882|Compound:0843-0131|FD-Score:3.63|P-value:1.14E-5 ID:SGTC_1033|Compound:k072-0102|FD-Score:3.07|P-value:1.71E-4 ID:SGTC_1035|Compound:k081-0041|FD-Score:3.51|P-value:2.17E-5 ID:SGTC_1036|Compound:k216-0215|FD-Score:-5.37|P-value:2.15E-10 ID:SGTC_1038|Compound:k292-0204|FD-Score:-4.50|P-value:8.79E-8 ID:SGTC_1082|Compound:camptothecin|FD-Score:-3.84|P-value:4.16E-6 ID:SGTC_1125|Compound:tnp00248|FD-Score:2.68|P-value:8.96E-4 ID:SGTC_1205|Compound:4161-2785|FD-Score:2.70|P-value:8.25E-4 ID:SGTC_1402|Compound:3909-7673|FD-Score:3.69|P-value:8.40E-6 ID:SGTC_1514|Compound:1189-1591|FD-Score:2.72|P-value:7.59E-4 ID:SGTC_1674|Compound:st016616|FD-Score:-3.46|P-value:2.96E-5 ID:SGTC_1682|Compound:st012968|FD-Score:-3.08|P-value:1.74E-4 ID:SGTC_1772|Compound:st048439|FD-Score:2.81|P-value:5.28E-4 ID:SGTC_1851|Compound:st056236|FD-Score:3.45|P-value:2.89E-5 ID:SGTC_1854|Compound:st056233|FD-Score:2.94|P-value:3.05E-4 ID:SGTC_1855|Compound:st056195|FD-Score:4.32|P-value:2.32E-7 ID:SGTC_1858|Compound:4-chloro-8-(diethylamino)pyrano[3,2-c]chromene-2,5-dione|FD-Score:3.46|P-value:2.71E-5 ID:SGTC_1861|Compound:st057647|FD-Score:4.65|P-value:2.94E-8 ID:SGTC_2087|Compound:5276107|FD-Score:-3.40|P-value:3.88E-5 ID:SGTC_2091|Compound:4-phenylthiadiazole|FD-Score:-2.90|P-value:3.82E-4 ID:SGTC_2120|Compound:5538279|FD-Score:-2.87|P-value:4.26E-4 ID:SGTC_2187|Compound:6125751|FD-Score:2.72|P-value:7.61E-4 ID:SGTC_2323|Compound:6148992|FD-Score:-3.27|P-value:7.47E-5 ID:SGTC_2461|Compound:5755302|FD-Score:2.65|P-value:9.89E-4 ID:SGTC_2464|Compound:5717415|FD-Score:-3.16|P-value:1.23E-4 ID:SGTC_2484|Compound:5809818|FD-Score:-2.82|P-value:5.35E-4 ID:SGTC_2564|Compound:betulinic acid|FD-Score:-3.57|P-value:1.74E-5 ID:SGTC_2598|Compound:perillyl alcohol|FD-Score:2.72|P-value:7.39E-4 ID:SGTC_2794|Compound:7785382|FD-Score:8.62|P-value:4.92E-24 ID:SGTC_2809|Compound:7964133|FD-Score:3.06|P-value:1.81E-4 ID:SGTC_2811|Compound:7967822|FD-Score:-2.67|P-value:9.63E-4

Top fitness defect scores for YLR117C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2794 7785382 8.62 27.3 µM 4.92E-24 1 3.24E-18 1.42
2 SGTC_1861 st057647 4.65 17.4 µM 2.94E-8 3 1.64E-6 0.51
3 SGTC_1855 st056195 4.32 39.6 µM 2.32E-7 60S ribosome export 8 7.65E-6 0.36
4 SGTC_1402 3909-7673 3.69 35.9 µM 8.40E-6 3 1.12E-4 0.04
5 SGTC_882 0843-0131 3.63 351.0 µM 1.14E-5 13 1.40E-4 0.08
6 SGTC_1035 k081-0041 3.51 55.2 µM 2.17E-5 Golgi 9 2.27E-4 0.03
7 SGTC_1858 4-chloro-8-(diethylamino)pyrano[3,2-c]chromene-2,5-dione 3.46 62.6 µM 2.71E-5 TSC3-RPN4 2 2.68E-4 0.16
8 SGTC_1851 st056236 3.45 18.9 µM 2.89E-5 10 2.82E-4 0.07
9 SGTC_169 k015-0017 3.44 16.0 µM 3.00E-5 5 2.90E-4 0.01
10 SGTC_276 colchicine 3.41 750.0 µM 3.41E-5 6 3.19E-4 0.12
11 SGTC_416 estradiol valerate 3.29 100.0 µM 6.33E-5 10 5.07E-4 4.47E-4
12 SGTC_859 0439-0102 3.20 54.6 µM 9.56E-5 redox potentiating 20 6.92E-4 0.07
13 SGTC_1033 k072-0102 3.07 21.3 µM 1.71E-4 60S ribosome export 14 0.00107 0.12
14 SGTC_595 3909-8003 3.07 73.7 µM 1.73E-4 12 0.00108 0.01
15 SGTC_2809 7964133 3.06 13.0 µM 1.81E-4 60S ribosome export 21 0.00112 0.01
16 SGTC_1854 st056233 2.94 32.9 µM 3.05E-4 amide catabolism 12 0.00165 0.08
17 SGTC_277 nystatin 2.89 15.5 µM 3.80E-4 10 0.00195 0.13
18 SGTC_1772 st048439 2.81 82.2 µM 5.28E-4 18 0.00250 0.04
19 SGTC_164 4100-3994 2.76 89.6 µM 6.51E-4 22 0.00293 0.06
20 SGTC_443 clozapine 2.74 122.0 µM 6.93E-4 NEO1-PIK1 12 0.00307 0.05

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.211 3.52E-35 YER013W PRP22 het DEAH-box RNA-dependent ATPase/ATP-dependent RNA helicase, associates with lariat intermediates before the second catalytic step of splicing; mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes
0.208 4.95E-34 YOR159C SME1 het Core Sm protein Sm E; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm E
0.196 1.70E-30 YKL024C URA6 het Uridylate kinase, catalyzes the seventh enzymatic step in the de novo biosynthesis of pyrimidines, converting uridine monophosphate (UMP) into uridine-5'-diphosphate (UDP)
0.182 2.43E-26 YJL173C RFA3 het Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved single-stranded DNA binding protein complex involved in DNA replication, repair, and recombination; protein abundance increases in response to DNA replication stress
0.177 4.99E-25 YHR166C CDC23 het Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition
0.173 5.25E-24 YOR304W ISW2 hom ATP-dependent DNA translocase involved in chromatin remodeling; ATPase component that, with Itc1p, forms a complex required for repression of a-specific genes, INO1, and early meiotic genes during mitotic growth
0.172 7.80E-24 YGR140W CBF2 het Essential kinetochore protein; component of the CBF3 multisubunit complex that binds to the CDEIII region of the centromere; Cbf2p also binds to the CDEII region possibly forming a different multimeric complex, ubiquitinated in vivo; relative distribution to the spindle pole body decreases upon DNA replication stress
0.172 1.03E-23 YDL060W TSR1 het Protein required for processing of 20S pre-rRNA in the cytoplasm; associates with pre-40S ribosomal particles; inhibits the premature association of 60S subunits with assembling 40S subunits in the cytoplasm; similar to Bms1p; relocalizes from nucleus to cytoplasm upon DNA replication stress
0.168 9.23E-23 YDR088C SLU7 het RNA splicing factor, required for ATP-independent portion of 2nd catalytic step of spliceosomal RNA splicing; interacts with Prp18p; contains zinc knuckle domain
0.164 1.17E-21 YKL036C_d YKL036C_d het Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the essential gene UGP1 gene and uncharacterized ORF YKL037W
0.159 1.91E-20 YGR082W TOM20 het Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins
0.156 1.02E-19 YGR280C PXR1 het Essential protein involved in rRNA and snoRNA maturation; competes with TLC1 RNA for binding to Est2p, suggesting a role in negative regulation of telomerase; human homolog inhibits telomerase; contains a G-patch RNA interacting domain
0.150 1.96E-18 YNL161W CBK1 het Serine/threonine protein kinase of the the RAM signaling network; Ndr/LATS family member; binds regulatory subunit Mob2p; involved in regulation of cellular morphogenesis, polarized growth, and septum destruction; phosphorylation by Cbk1p regulates localization and activity of Ace2p transcription factor and Ssd1p translational repressor; Cbk1p activity is regulated by both phosphorylation and specific localization; relocalizes to cytoplasm upon DNA replication stress
0.149 5.08E-18 YJR072C NPA3 het Member of the conserved GPN-loop GTPase family; has a role in transport of RNA polymerase II to the nucleus; exhibits GTP-dependent binding to PolII; has ATPase activity; involved in sister chromatid cohesion; phosphorylated by the Pcl1p-Pho85p kinase complex; human homolog XAB1 interacts with human RNA polymerase II; protein abundance increases in response to DNA replication stress
0.147 9.84E-18 YMR132C_p JLP2_p hom Protein of unknown function, contains sequence that closely resembles a J domain (typified by the E. coli DnaJ protein)