HIPHOP chemogenomics database

YLR219W / MSC3

Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; msc3 mutants are defective in directing meiotic recombination events to homologous chromatids; potential Cdc28p substrate; protein abundance increases in response to DNA replication stress

Zygosity: Homozygous strain

fixedexpanded
Profile for YLR219W / MSC3

Click on Significant Values for Screen Details

Top fitness defect scores for YLR219W deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_551	k048-0028	5.37	34.9 µM	9.13E-10		2	4.03E-8
2	SGTC_869	0564-0080	4.82	44.1 µM	3.24E-8		9	7.20E-7
3	SGTC_2591	skatole	4.69	100.0 µM	7.35E-8		19	1.39E-6
4	SGTC_2351	9026858	4.68	72.3 µM	7.66E-8	RNA processing & uracil transport	15	1.44E-6
5	SGTC_3316	9136639	4.59	10.6 µM	1.30E-7		7	2.21E-6
6	SGTC_369	0957-0393	4.56	4.5 µM	1.55E-7	cell wall signaling	10	2.54E-6
7	SGTC_903	1134-0042	4.39	114.0 µM	4.28E-7		15	5.79E-6
8	SGTC_2622	2-methoxyxanthone	4.27	59.9 µM	8.28E-7		13	9.88E-6
9	SGTC_500	h-89	4.20	112.0 µM	1.22E-6	cell wall signaling	26	1.35E-5
10	SGTC_2418	pentamidine	4.08	31.7 µM	2.34E-6	mitochondrial processes	37	2.29E-5
11	SGTC_1739	st037196	3.95	92.5 µM	4.53E-6		23	3.90E-5
12	SGTC_2709	st078560	3.93	56.0 µM	4.92E-6		12	4.18E-5
13	SGTC_1206	3978-0017	3.89	132.0 µM	6.11E-6	ERG2	39	4.97E-5
14	SGTC_839	0122-0028	3.80	230.0 µM	9.56E-6	RSC complex & mRNA processing	43	7.14E-5
15	SGTC_372	0155-0182	3.57	15.9 µM	2.90E-5	mitochondrial response to ROS	41	1.75E-4
16	SGTC_693	4161-2696	3.54	114.0 µM	3.34E-5		17	1.97E-4
17	SGTC_362	0310-0100	3.47	9.0 µM	4.82E-5	RPP1 & pyrimidine depletion	52	2.65E-4
18	SGTC_2048	5246248	3.42	200.0 µM	5.98E-5		16	3.15E-4
19	SGTC_133	0724-0033	3.34	378.9 µM	8.65E-5		48	4.25E-4
20	SGTC_2405	ticlatone	3.25	3.5 µM	1.27E-4		110	5.82E-4

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.117	9.68E-12	YOR282W_d	YOR282W_d	het	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps essential, verified gene PLP2/YOR281C
0.105	9.04E-10	YKR018C	YKR018C	hom	Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress; YKR018C has a paralog, IML2, that arose from the whole genome duplication
0.084	1.09E-6	YER008C	SEC3	het	Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in a Rho1p-dependent, actin-independent manner, targeting and anchoring the exocyst to the plasma membrane with Exo70p; direct GTP Rho1p effector; required for ER inheritance; relocalizes away from bud neck upon DNA replication stress
0.082	2.24E-6	YLR024C	UBR2	hom	Cytoplasmic ubiquitin-protein ligase (E3); required for ubiquitylation of Rpn4p; mediates formation of a Mub1p-Ubr2p-Rad6p complex
0.081	2.41E-6	YPR008W	HAA1	hom	Transcriptional activator involved in adaptation to weak acid stress; activates transcription of TPO2, YRO2, and other genes encoding membrane stress proteins; HAA1 has a paralog, CUP2, that arose from the whole genome duplication; relocalizes from cytoplasm to nucleus upon DNA replication stress
0.081	2.50E-6	YHL040C	ARN1	hom	ARN family transporter for siderophore-iron chelates; responsible for uptake of iron bound to ferrirubin, ferrirhodin, and related siderophores; protein increases in abundance and relocalizes to the vacuole upon DNA replication stress
0.080	3.24E-6	YAL008W	FUN14	hom	Mitochondrial protein of unknown function
0.080	3.29E-6	YEL012W	UBC8	hom	Ubiquitin-conjugating enzyme that negatively regulates gluconeogenesis by mediating the glucose-induced ubiquitination of fructose-1,6-bisphosphatase (FBPase); cytoplasmic enzyme that catalyzes the ubiquitination of histones in vitro
0.080	4.08E-6	YER049W	TPA1	hom	Poly(rA)-binding protein involved in translation termination efficiency, mRNA poly(A) tail length and mRNA stability; interacts with Sup45p (eRF1), Sup35p (eRF3) and Pab1p; similar to prolyl 4-hydroxylases; binds Fe(III) and 2-oxoglutarate
0.079	4.44E-6	YHR014W	SPO13	hom	Meiosis-specific protein, involved in maintaining sister chromatid cohesion during meiosis I as well as promoting proper attachment of kinetochores to the spindle during meiosis I and meiosis II
0.079	4.61E-6	YFL051C_p	YFL051C_p	hom	Putative protein of unknown function; YFL051C is not an essential gene
0.079	5.00E-6	YDR341C	YDR341C	het	Arginyl-tRNA synthetase, proposed to be cytoplasmic but the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.077	7.22E-6	YBR136W	MEC1	het	Genome integrity checkpoint protein and PI kinase superfamily member; signal transducer required for cell cycle arrest and transcriptional responses prompted by damaged or unreplicated DNA; regulates P-body formation induced by replication stress; monitors and participates in meiotic recombination; associates with shortened, dysfunctional telomeres
0.077	8.10E-6	YOL151W	GRE2	hom	3-methylbutanal reductase and NADPH-dependent methylglyoxal reductase (D-lactaldehyde dehydrogenase); stress induced (osmotic, ionic, oxidative, heat shock and heavy metals); regulated by the HOG pathway; protein abundance increases in response to DNA replication stress
0.077	9.07E-6	YDR009W	GAL3	hom	Transcriptional regulator; involved in activation of the GAL genes in response to galactose; forms a complex with Gal80p to relieve Gal80p inhibition of Gal4p; binds galactose and ATP but does not have galactokinase activity; GAL3 has a paralog, GAL1, that arose from the whole genome duplication