YLR250W / SSP120

Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern

Zygosity: Homozygous strain
fixedexpanded
Profile for YLR250W / SSP120

Click on Significant Values for Screen Details ID:SGTC_6|Compound:0844-0013|FD-Score:3.63|P-value:1.09E-4 ID:SGTC_99|Compound:0109-0120|FD-Score:3.76|P-value:6.50E-5 ID:SGTC_162|Compound:k030-0015|FD-Score:3.09|P-value:8.42E-4 ID:SGTC_166|Compound:k072-0058|FD-Score:-4.18|P-value:8.50E-6 ID:SGTC_460|Compound:trequinsin|FD-Score:3.09|P-value:8.23E-4 ID:SGTC_510|Compound:ag-490|FD-Score:3.19|P-value:5.91E-4 ID:SGTC_690|Compound:glyoxal|FD-Score:3.06|P-value:9.29E-4 ID:SGTC_760|Compound:0568-0725|FD-Score:3.56|P-value:1.42E-4 ID:SGTC_839|Compound:0122-0028|FD-Score:4.12|P-value:1.32E-5 ID:SGTC_985|Compound:1493-0307|FD-Score:3.57|P-value:1.41E-4 ID:SGTC_1161|Compound:k007-0610|FD-Score:-3.29|P-value:3.58E-4 ID:SGTC_1242|Compound:0402-0103|FD-Score:3.07|P-value:8.81E-4 ID:SGTC_1403|Compound:3909-7815|FD-Score:-3.08|P-value:7.67E-4 ID:SGTC_1462|Compound:k081-0014|FD-Score:-3.04|P-value:8.75E-4 ID:SGTC_1506|Compound:4226-2149|FD-Score:-3.11|P-value:6.71E-4 ID:SGTC_1514|Compound:1189-1591|FD-Score:4.10|P-value:1.49E-5 ID:SGTC_1515|Compound:3460-0031|FD-Score:-3.07|P-value:7.96E-4 ID:SGTC_1517|Compound:deoxycholic acid methyl ester|FD-Score:-3.21|P-value:4.71E-4 ID:SGTC_1542|Compound:7-hydroxy-3'-methoxyflavone|FD-Score:3.42|P-value:2.46E-4 ID:SGTC_1610|Compound:st001799|FD-Score:-4.91|P-value:2.28E-7 ID:SGTC_1618|Compound:st003135|FD-Score:3.98|P-value:2.53E-5 ID:SGTC_1725|Compound:st036284|FD-Score:6.77|P-value:2.44E-12 ID:SGTC_1863|Compound:st057641|FD-Score:-3.25|P-value:4.13E-4 ID:SGTC_1867|Compound:st057372|FD-Score:-3.46|P-value:1.82E-4 ID:SGTC_1868|Compound:st057644|FD-Score:-4.21|P-value:7.46E-6 ID:SGTC_1899|Compound:st063254|FD-Score:-3.29|P-value:3.48E-4 ID:SGTC_2361|Compound:9034121|FD-Score:-3.05|P-value:8.32E-4 ID:SGTC_2638|Compound:epoxomicin|FD-Score:-3.15|P-value:5.94E-4 ID:SGTC_2719|Compound:novobiocin|FD-Score:3.69|P-value:8.57E-5 ID:SGTC_2720|Compound:fluspirilene|FD-Score:3.22|P-value:5.20E-4 ID:SGTC_2829|Compound:7992743|FD-Score:-3.41|P-value:2.22E-4 ID:SGTC_2846|Compound:9008642|FD-Score:3.49|P-value:1.88E-4 ID:SGTC_2949|Compound:9072542|FD-Score:-7.39|P-value:1.66E-14 ID:SGTC_2960|Compound:9079389|FD-Score:-5.51|P-value:7.66E-9 ID:SGTC_3196|Compound:9111891|FD-Score:4.70|P-value:8.33E-7 ID:SGTC_3319|Compound:9138450|FD-Score:-3.54|P-value:1.33E-4 ID:SGTC_3333|Compound:9141820|FD-Score:3.45|P-value:2.25E-4 ID:SGTC_6|Compound:0844-0013|FD-Score:3.63|P-value:1.09E-4 ID:SGTC_99|Compound:0109-0120|FD-Score:3.76|P-value:6.50E-5 ID:SGTC_162|Compound:k030-0015|FD-Score:3.09|P-value:8.42E-4 ID:SGTC_166|Compound:k072-0058|FD-Score:-4.18|P-value:8.50E-6 ID:SGTC_460|Compound:trequinsin|FD-Score:3.09|P-value:8.23E-4 ID:SGTC_510|Compound:ag-490|FD-Score:3.19|P-value:5.91E-4 ID:SGTC_690|Compound:glyoxal|FD-Score:3.06|P-value:9.29E-4 ID:SGTC_760|Compound:0568-0725|FD-Score:3.56|P-value:1.42E-4 ID:SGTC_839|Compound:0122-0028|FD-Score:4.12|P-value:1.32E-5 ID:SGTC_985|Compound:1493-0307|FD-Score:3.57|P-value:1.41E-4 ID:SGTC_1161|Compound:k007-0610|FD-Score:-3.29|P-value:3.58E-4 ID:SGTC_1242|Compound:0402-0103|FD-Score:3.07|P-value:8.81E-4 ID:SGTC_1403|Compound:3909-7815|FD-Score:-3.08|P-value:7.67E-4 ID:SGTC_1462|Compound:k081-0014|FD-Score:-3.04|P-value:8.75E-4 ID:SGTC_1506|Compound:4226-2149|FD-Score:-3.11|P-value:6.71E-4 ID:SGTC_1514|Compound:1189-1591|FD-Score:4.10|P-value:1.49E-5 ID:SGTC_1515|Compound:3460-0031|FD-Score:-3.07|P-value:7.96E-4 ID:SGTC_1517|Compound:deoxycholic acid methyl ester|FD-Score:-3.21|P-value:4.71E-4 ID:SGTC_1542|Compound:7-hydroxy-3'-methoxyflavone|FD-Score:3.42|P-value:2.46E-4 ID:SGTC_1610|Compound:st001799|FD-Score:-4.91|P-value:2.28E-7 ID:SGTC_1618|Compound:st003135|FD-Score:3.98|P-value:2.53E-5 ID:SGTC_1725|Compound:st036284|FD-Score:6.77|P-value:2.44E-12 ID:SGTC_1863|Compound:st057641|FD-Score:-3.25|P-value:4.13E-4 ID:SGTC_1867|Compound:st057372|FD-Score:-3.46|P-value:1.82E-4 ID:SGTC_1868|Compound:st057644|FD-Score:-4.21|P-value:7.46E-6 ID:SGTC_1899|Compound:st063254|FD-Score:-3.29|P-value:3.48E-4 ID:SGTC_2361|Compound:9034121|FD-Score:-3.05|P-value:8.32E-4 ID:SGTC_2638|Compound:epoxomicin|FD-Score:-3.15|P-value:5.94E-4 ID:SGTC_2719|Compound:novobiocin|FD-Score:3.69|P-value:8.57E-5 ID:SGTC_2720|Compound:fluspirilene|FD-Score:3.22|P-value:5.20E-4 ID:SGTC_2829|Compound:7992743|FD-Score:-3.41|P-value:2.22E-4 ID:SGTC_2846|Compound:9008642|FD-Score:3.49|P-value:1.88E-4 ID:SGTC_2949|Compound:9072542|FD-Score:-7.39|P-value:1.66E-14 ID:SGTC_2960|Compound:9079389|FD-Score:-5.51|P-value:7.66E-9 ID:SGTC_3196|Compound:9111891|FD-Score:4.70|P-value:8.33E-7 ID:SGTC_3319|Compound:9138450|FD-Score:-3.54|P-value:1.33E-4 ID:SGTC_3333|Compound:9141820|FD-Score:3.45|P-value:2.25E-4

Top fitness defect scores for YLR250W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1725 st036284 6.77 20.6 µM 2.44E-12 3 6.59E-12
2 SGTC_3196 9111891 4.70 49.5 µM 8.33E-7 mitochondrial processes 8 1.32E-6
3 SGTC_839 0122-0028 4.12 230.0 µM 1.32E-5 RSC complex & mRNA processing 35 1.87E-5
4 SGTC_1514 1189-1591 4.10 158.0 µM 1.49E-5 30 2.10E-5
5 SGTC_1618 st003135 3.98 44.5 µM 2.53E-5 19 3.49E-5
6 SGTC_99 0109-0120 3.76 622.8 µM 6.50E-5 20 8.64E-5
7 SGTC_2719 novobiocin 3.69 32.6 µM 8.57E-5 5 1.13E-4
8 SGTC_6 0844-0013 3.63 19.8 µM 1.09E-4 122 1.42E-4
9 SGTC_985 1493-0307 3.57 8.7 µM 1.41E-4 TSC3-RPN4 28 1.81E-4
10 SGTC_760 0568-0725 3.56 82.9 µM 1.42E-4 18 1.83E-4
11 SGTC_2846 9008642 3.49 19.5 µM 1.88E-4 26 2.40E-4
12 SGTC_3333 9141820 3.45 12.1 µM 2.25E-4 25 2.85E-4
13 SGTC_1542 7-hydroxy-3'-methoxyflavone 3.42 49.7 µM 2.46E-4 29 3.11E-4
14 SGTC_2720 fluspirilene 3.22 36.9 µM 5.20E-4 ERG2 32 6.37E-4
15 SGTC_510 ag-490 3.19 170.0 µM 5.91E-4 15 7.21E-4
16 SGTC_460 trequinsin 3.09 123.0 µM 8.23E-4 11 9.92E-4
17 SGTC_162 k030-0015 3.09 37.8 µM 8.42E-4 iron homeostasis 46 0.00101
18 SGTC_1242 0402-0103 3.07 60.1 µM 8.81E-4 63 0.00106
19 SGTC_690 glyoxal 3.06 20.3 µM 9.29E-4 143 0.00111
20 SGTC_3197 9113331 3.03 49.5 µM 0.00102 90 0.00122

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.156 9.03E-20 YBR274W CHK1 hom Serine/threonine kinase and DNA damage checkpoint effector, mediates cell cycle arrest via phosphorylation of Pds1p; phosphorylated by checkpoint signal transducer Mec1p; homolog of S. pombe and mammalian Chk1 checkpoint kinase
0.135 4.98E-15 YLR257W YLR257W hom Protein of unknown function; protein abundance increases in response to DNA replication stress
0.121 2.46E-12 YJR052W RAD7 hom Protein that recognizes and binds damaged DNA in an ATP-dependent manner (with Rad16p) during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 4 (NEF4) and the Elongin-Cullin-Socs (ECS) ligase complex
0.102 3.55E-9 YMR144W_p YMR144W_p hom Putative protein of unknown function; localized to the nucleus; YMR144W is not an essential gene
0.101 5.23E-9 YNL303W_d YNL303W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.100 6.56E-9 YGR036C CAX4 hom Dolichyl pyrophosphate (Dol-P-P) phosphatase with a luminally oriented active site in the ER, cleaves the anhydride linkage in Dol-P-P, required for Dol-P-P-linked oligosaccharide intermediate synthesis and protein N-glycosylation
0.097 1.53E-8 YDR068W DOS2 hom Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.097 1.87E-8 YJR019C TES1 hom Peroxisomal acyl-CoA thioesterase likely to be involved in fatty acid oxidation rather than fatty acid synthesis; conserved protein also found in human peroxisomes; TES1 mRNA levels increase during growth on fatty acids
0.095 3.10E-8 YIL056W VHR1 hom Transcriptional activator; required for the vitamin H-responsive element (VHRE) mediated induction of VHT1 (Vitamin H transporter) and BIO5 (biotin biosynthesis intermediate transporter) in response to low biotin concentrations; VHR1 has a paralog, VHR2, that arose from the whole genome duplication
0.094 4.66E-8 YDL029W ARP2 het Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity
0.088 3.49E-7 YOR261C RPN8 het Essential, non-ATPase regulatory subunit of the 26S proteasome; has similarity to the human p40 proteasomal subunit and to another S. cerevisiae regulatory subunit, Rpn11p
0.086 6.07E-7 YOR155C ISN1 hom Inosine 5'-monophosphate (IMP)-specific 5'-nucleotidase, catalyzes the breakdown of IMP to inosine, does not show similarity to known 5'-nucleotidases from other organisms
0.082 1.75E-6 YML057W CMP2 hom Calcineurin A; one isoform (the other is CNA1) of the catalytic subunit of calcineurin, a Ca++/calmodulin-regulated protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is CNB1
0.082 1.85E-6 YGL110C CUE3 hom Protein of unknown function; has a CUE domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination
0.082 1.89E-6 YNL338W_d YNL338W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps TEL14L-XC, which is Telomeric X element Core sequence on the left arm of Chromosome XIV