YLR264W / RPS28B

Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S28, no bacterial homolog; RPS28B has a paralog, RPS28A, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YLR264W / RPS28B

Click on Significant Values for Screen Details ID:SGTC_118|Compound:0083-0097|FD-Score:3.49|P-value:4.02E-4 ID:SGTC_123|Compound:0083-0120|FD-Score:4.03|P-value:5.70E-5 ID:SGTC_170|Compound:4197-0004|FD-Score:3.64|P-value:2.42E-4 ID:SGTC_260|Compound:nsc-65238|FD-Score:3.41|P-value:5.19E-4 ID:SGTC_309|Compound:k408-0051|FD-Score:3.37|P-value:6.00E-4 ID:SGTC_346|Compound:4513-0042|FD-Score:-3.70|P-value:3.35E-4 ID:SGTC_422|Compound:cantharidin|FD-Score:3.78|P-value:1.42E-4 ID:SGTC_497|Compound:capsazepine|FD-Score:-3.44|P-value:7.79E-4 ID:SGTC_625|Compound:k213-0058|FD-Score:3.65|P-value:2.32E-4 ID:SGTC_752|Compound:1896-0090|FD-Score:3.89|P-value:9.70E-5 ID:SGTC_800|Compound:3448-7362|FD-Score:4.69|P-value:3.65E-6 ID:SGTC_999|Compound:4022-0066|FD-Score:3.22|P-value:9.72E-4 ID:SGTC_1006|Compound:4099-6547|FD-Score:6.91|P-value:2.52E-11 ID:SGTC_1046|Compound:2001-0062|FD-Score:4.21|P-value:2.76E-5 ID:SGTC_1085|Compound:doxorubicin|FD-Score:3.66|P-value:2.24E-4 ID:SGTC_1179|Compound:1021-1431|FD-Score:3.72|P-value:1.77E-4 ID:SGTC_1182|Compound:1222-0449|FD-Score:4.68|P-value:3.82E-6 ID:SGTC_1185|Compound:1319-0113|FD-Score:3.45|P-value:4.53E-4 ID:SGTC_1233|Compound:0325-0408|FD-Score:3.27|P-value:8.31E-4 ID:SGTC_1456|Compound:k015-0012|FD-Score:-3.44|P-value:7.87E-4 ID:SGTC_1457|Compound:k015-0032|FD-Score:4.17|P-value:3.31E-5 ID:SGTC_1609|Compound:st001574|FD-Score:3.59|P-value:2.82E-4 ID:SGTC_1613|Compound:st002381|FD-Score:3.48|P-value:4.12E-4 ID:SGTC_1652|Compound:st011932|FD-Score:-3.52|P-value:6.04E-4 ID:SGTC_1781|Compound:st038725|FD-Score:4.32|P-value:1.77E-5 ID:SGTC_2602|Compound:kawain|FD-Score:-3.37|P-value:9.88E-4 ID:SGTC_2640|Compound:pentamidine|FD-Score:-5.23|P-value:6.56E-7 ID:SGTC_2688|Compound:kanamycin b|FD-Score:5.74|P-value:2.24E-8 ID:SGTC_2792|Compound:7535235|FD-Score:-3.83|P-value:2.15E-4 ID:SGTC_2797|Compound:7859098|FD-Score:-4.16|P-value:6.31E-5 ID:SGTC_3018|Compound:9082602|FD-Score:-3.62|P-value:4.44E-4 ID:SGTC_3199|Compound:9111318|FD-Score:-3.79|P-value:2.45E-4 ID:SGTC_3238|Compound:9133470|FD-Score:-4.54|P-value:1.40E-5 ID:SGTC_3332|Compound:9141069|FD-Score:-3.39|P-value:9.27E-4 ID:SGTC_118|Compound:0083-0097|FD-Score:3.49|P-value:4.02E-4 ID:SGTC_123|Compound:0083-0120|FD-Score:4.03|P-value:5.70E-5 ID:SGTC_170|Compound:4197-0004|FD-Score:3.64|P-value:2.42E-4 ID:SGTC_260|Compound:nsc-65238|FD-Score:3.41|P-value:5.19E-4 ID:SGTC_309|Compound:k408-0051|FD-Score:3.37|P-value:6.00E-4 ID:SGTC_346|Compound:4513-0042|FD-Score:-3.70|P-value:3.35E-4 ID:SGTC_422|Compound:cantharidin|FD-Score:3.78|P-value:1.42E-4 ID:SGTC_497|Compound:capsazepine|FD-Score:-3.44|P-value:7.79E-4 ID:SGTC_625|Compound:k213-0058|FD-Score:3.65|P-value:2.32E-4 ID:SGTC_752|Compound:1896-0090|FD-Score:3.89|P-value:9.70E-5 ID:SGTC_800|Compound:3448-7362|FD-Score:4.69|P-value:3.65E-6 ID:SGTC_999|Compound:4022-0066|FD-Score:3.22|P-value:9.72E-4 ID:SGTC_1006|Compound:4099-6547|FD-Score:6.91|P-value:2.52E-11 ID:SGTC_1046|Compound:2001-0062|FD-Score:4.21|P-value:2.76E-5 ID:SGTC_1085|Compound:doxorubicin|FD-Score:3.66|P-value:2.24E-4 ID:SGTC_1179|Compound:1021-1431|FD-Score:3.72|P-value:1.77E-4 ID:SGTC_1182|Compound:1222-0449|FD-Score:4.68|P-value:3.82E-6 ID:SGTC_1185|Compound:1319-0113|FD-Score:3.45|P-value:4.53E-4 ID:SGTC_1233|Compound:0325-0408|FD-Score:3.27|P-value:8.31E-4 ID:SGTC_1456|Compound:k015-0012|FD-Score:-3.44|P-value:7.87E-4 ID:SGTC_1457|Compound:k015-0032|FD-Score:4.17|P-value:3.31E-5 ID:SGTC_1609|Compound:st001574|FD-Score:3.59|P-value:2.82E-4 ID:SGTC_1613|Compound:st002381|FD-Score:3.48|P-value:4.12E-4 ID:SGTC_1652|Compound:st011932|FD-Score:-3.52|P-value:6.04E-4 ID:SGTC_1781|Compound:st038725|FD-Score:4.32|P-value:1.77E-5 ID:SGTC_2602|Compound:kawain|FD-Score:-3.37|P-value:9.88E-4 ID:SGTC_2640|Compound:pentamidine|FD-Score:-5.23|P-value:6.56E-7 ID:SGTC_2688|Compound:kanamycin b|FD-Score:5.74|P-value:2.24E-8 ID:SGTC_2792|Compound:7535235|FD-Score:-3.83|P-value:2.15E-4 ID:SGTC_2797|Compound:7859098|FD-Score:-4.16|P-value:6.31E-5 ID:SGTC_3018|Compound:9082602|FD-Score:-3.62|P-value:4.44E-4 ID:SGTC_3199|Compound:9111318|FD-Score:-3.79|P-value:2.45E-4 ID:SGTC_3238|Compound:9133470|FD-Score:-4.54|P-value:1.40E-5 ID:SGTC_3332|Compound:9141069|FD-Score:-3.39|P-value:9.27E-4

Top fitness defect scores for YLR264W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1006 4099-6547 6.91 255.0 µM 2.52E-11 2 2.36E-12
2 SGTC_2688 kanamycin b 5.74 96.7 µM 2.24E-8 calcium & mitochondrial duress 173 4.61E-9
3 SGTC_800 3448-7362 4.69 155.0 µM 3.65E-6 translation 104 1.33E-6
4 SGTC_1182 1222-0449 4.68 220.0 µM 3.82E-6 7 1.40E-6
5 SGTC_1781 st038725 4.32 66.3 µM 1.77E-5 NEO1 88 7.68E-6
6 SGTC_1046 2001-0062 4.21 216.0 µM 2.76E-5 13 1.25E-5
7 SGTC_1457 k015-0032 4.17 57.3 µM 3.31E-5 32 1.54E-5
8 SGTC_123 0083-0120 4.03 66.7 µM 5.70E-5 46 2.80E-5
9 SGTC_752 1896-0090 3.89 130.0 µM 9.70E-5 TSC3-RPN4 19 5.05E-5
10 SGTC_422 cantharidin 3.78 155.0 µM 1.42E-4 cell wall signaling 64 7.69E-5
11 SGTC_1179 1021-1431 3.72 9.3 µM 1.77E-4 51 9.81E-5
12 SGTC_1085 doxorubicin 3.66 7.7 µM 2.24E-4 anthracycline transcription coupled DNA repair 28 1.27E-4
13 SGTC_625 k213-0058 3.65 121.0 µM 2.32E-4 translation 131 1.32E-4
14 SGTC_170 4197-0004 3.64 226.0 µM 2.42E-4 25 1.38E-4
15 SGTC_1609 st001574 3.59 46.4 µM 2.82E-4 21 1.64E-4
16 SGTC_118 0083-0097 3.49 135.3 µM 4.02E-4 translation 188 2.43E-4
17 SGTC_1613 st002381 3.48 52.1 µM 4.12E-4 35 2.49E-4
18 SGTC_1185 1319-0113 3.45 81.3 µM 4.53E-4 DNA damage response 21 2.77E-4
19 SGTC_260 nsc-65238 3.41 4.0 µM 5.19E-4 endomembrane recycling 30 3.22E-4
20 SGTC_309 k408-0051 3.37 1.5 µM 6.00E-4 89 3.77E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.185 3.20E-27 YJL136C RPS21B hom Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S21, no bacterial homolog; RPS21B has a paralog, RPS21A, that arose from the whole genome duplication
0.170 4.00E-23 YLR048W RPS0B hom Protein component of the small (40S) ribosomal subunit; RPS0B has a paralog, RPS0A, that arose from the whole genome duplication; required for maturation of 18S rRNA along with Rps0Ap; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal; homologous to human ribosomal protein SA and bacterial S2
0.148 7.87E-18 YGR162W TIF4631 hom Translation initiation factor eIF4G; subunit of the mRNA cap-binding protein complex (eIF4F) that also contains eIF4E (Cdc33p); interacts with Pab1p and with eIF4A (Tif1p); also has a role in biogenesis of the large ribosomal subunit; TIF4631 has a paralog, TIF4632, that arose from the whole genome duplication
0.146 1.84E-17 YDR140W MTQ2 hom S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; subunit of complex with Trm112p that methylates translation release factor Sup45p (eRF1) in the ternary complex eRF1-eRF3-GTP; similar to E.coli PrmC
0.145 2.83E-17 YJL189W RPL39 hom Ribosomal 60S subunit protein L39; required for ribosome biogenesis; loss of both Rpl31p and Rpl39p confers lethality; also exhibits genetic interactions with SIS1 and PAB1; homologous to mammalian ribosomal protein L39, no bacterial homolog
0.141 2.95E-16 YJL188C_d BUD19_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 88% of ORF overlaps the verified gene RPL39; diploid mutant displays a weak budding pattern phenotype in a systematic assay
0.141 2.95E-16 YOR235W_d IRC13_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays increased levels of spontaneous Rad52 foci
0.139 5.46E-16 YBL071C-B_p YBL071C-B_p hom Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
0.136 2.21E-15 YOR309C_d YOR309C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP58
0.131 2.44E-14 YOR293W RPS10A hom Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S10, no bacterial homolog; RPS10A has a paralog, RPS10B, that arose from the whole genome duplication
0.130 3.78E-14 YJR145C RPS4A hom Protein component of the small (40S) ribosomal subunit; mutation affects 20S pre-rRNA processing; homologous to mammalian ribosomal protein S4, no bacterial homolog; RPS4A has a paralog, RPS4B, that arose from the whole genome duplication
0.130 4.21E-14 YOR096W RPS7A hom Protein component of the small (40S) ribosomal subunit; interacts with Kti11p; deletion causes hypersensitivity to zymocin; homologous to mammalian ribosomal protein S7, no bacterial homolog; RPS7A has a paralog, RPS7B, that arose from the whole genome duplication
0.130 5.07E-14 YDR025W RPS11A hom Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S11 and bacterial S17; RPS11A has a paralog, RPS11B, that arose from the whole genome duplication
0.130 4.96E-14 YGR271C-A EFG1 hom Essential protein required for maturation of 18S rRNA; null mutant is sensitive to hydroxyurea and is delayed in recovering from alpha-factor arrest; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus
0.129 5.39E-14 YGR204W ADE3 hom Cytoplasmic trifunctional enzyme C1-tetrahydrofolate synthase, involved in single carbon metabolism and required for biosynthesis of purines, thymidylate, methionine, and histidine; null mutation causes auxotrophy for adenine and histidine