YLR265C / NEJ1

Protein involved in regulation of nonhomologous end joining; interacts with DNA ligase IV components Dnl4p and Lif1p; repressed by MAT heterozygosity; regulates cellular distribution of Lif1p

Zygosity: Homozygous strain
fixedexpanded
Profile for YLR265C / NEJ1

Click on Significant Values for Screen Details ID:SGTC_498|Compound:2,5-di-tert-butyl-1,4-hydroquinone (DTBHQ)|FD-Score:-3.32|P-value:3.15E-4 ID:SGTC_500|Compound:h-89|FD-Score:3.32|P-value:2.79E-4 ID:SGTC_501|Compound:nsc-95397|FD-Score:3.94|P-value:2.11E-5 ID:SGTC_504|Compound:lovastatin|FD-Score:4.25|P-value:5.03E-6 ID:SGTC_509|Compound:pifithrin|FD-Score:-3.20|P-value:4.93E-4 ID:SGTC_644|Compound:1498-1197|FD-Score:5.14|P-value:4.57E-8 ID:SGTC_700|Compound:3453-2283|FD-Score:3.66|P-value:7.12E-5 ID:SGTC_711|Compound:k292-0785|FD-Score:-3.02|P-value:9.32E-4 ID:SGTC_725|Compound:1497-0963|FD-Score:3.53|P-value:1.19E-4 ID:SGTC_798|Compound:1287-0182|FD-Score:3.16|P-value:4.98E-4 ID:SGTC_955|Compound:1216-0110|FD-Score:-3.26|P-value:3.87E-4 ID:SGTC_1149|Compound:3909-7961|FD-Score:3.85|P-value:3.04E-5 ID:SGTC_1171|Compound:0368-0077|FD-Score:4.35|P-value:3.09E-6 ID:SGTC_1386|Compound:3228-0095|FD-Score:3.01|P-value:8.51E-4 ID:SGTC_1404|Compound:3909-7820|FD-Score:3.06|P-value:7.19E-4 ID:SGTC_1775|Compound:st081588|FD-Score:3.58|P-value:9.79E-5 ID:SGTC_1782|Compound:st039423|FD-Score:3.78|P-value:4.14E-5 ID:SGTC_1783|Compound:st039459|FD-Score:5.80|P-value:8.68E-10 ID:SGTC_1966|Compound:st070145|FD-Score:3.19|P-value:4.53E-4 ID:SGTC_2101|Compound:5377231|FD-Score:3.80|P-value:3.90E-5 ID:SGTC_2334|Compound:9017922|FD-Score:3.14|P-value:5.36E-4 ID:SGTC_2398|Compound:5228213|FD-Score:-3.19|P-value:5.05E-4 ID:SGTC_2405|Compound:ticlatone|FD-Score:-4.86|P-value:2.65E-7 ID:SGTC_2416|Compound:tofa|FD-Score:-3.53|P-value:1.39E-4 ID:SGTC_2536|Compound:dehydroabietamide|FD-Score:-3.64|P-value:8.84E-5 ID:SGTC_2822|Compound:7994718|FD-Score:3.82|P-value:3.58E-5 ID:SGTC_2923|Compound:7992654|FD-Score:3.15|P-value:5.18E-4 ID:SGTC_2974|Compound:9094440|FD-Score:-3.41|P-value:2.21E-4 ID:SGTC_2999|Compound:9069765|FD-Score:-3.45|P-value:1.88E-4 ID:SGTC_3136|Compound:9095672|FD-Score:-5.25|P-value:3.06E-8 ID:SGTC_3145|Compound:9085516|FD-Score:4.24|P-value:5.21E-6 ID:SGTC_3202|Compound:9113761|FD-Score:-3.39|P-value:2.43E-4 ID:SGTC_3277|Compound:9138724|FD-Score:-3.13|P-value:6.35E-4 ID:SGTC_3283|Compound:9109332|FD-Score:-3.85|P-value:3.59E-5 ID:SGTC_3284|Compound:9108171|FD-Score:-3.12|P-value:6.50E-4 ID:SGTC_3316|Compound:9136639|FD-Score:3.64|P-value:7.54E-5 ID:SGTC_498|Compound:2,5-di-tert-butyl-1,4-hydroquinone (DTBHQ)|FD-Score:-3.32|P-value:3.15E-4 ID:SGTC_500|Compound:h-89|FD-Score:3.32|P-value:2.79E-4 ID:SGTC_501|Compound:nsc-95397|FD-Score:3.94|P-value:2.11E-5 ID:SGTC_504|Compound:lovastatin|FD-Score:4.25|P-value:5.03E-6 ID:SGTC_509|Compound:pifithrin|FD-Score:-3.20|P-value:4.93E-4 ID:SGTC_644|Compound:1498-1197|FD-Score:5.14|P-value:4.57E-8 ID:SGTC_700|Compound:3453-2283|FD-Score:3.66|P-value:7.12E-5 ID:SGTC_711|Compound:k292-0785|FD-Score:-3.02|P-value:9.32E-4 ID:SGTC_725|Compound:1497-0963|FD-Score:3.53|P-value:1.19E-4 ID:SGTC_798|Compound:1287-0182|FD-Score:3.16|P-value:4.98E-4 ID:SGTC_955|Compound:1216-0110|FD-Score:-3.26|P-value:3.87E-4 ID:SGTC_1149|Compound:3909-7961|FD-Score:3.85|P-value:3.04E-5 ID:SGTC_1171|Compound:0368-0077|FD-Score:4.35|P-value:3.09E-6 ID:SGTC_1386|Compound:3228-0095|FD-Score:3.01|P-value:8.51E-4 ID:SGTC_1404|Compound:3909-7820|FD-Score:3.06|P-value:7.19E-4 ID:SGTC_1775|Compound:st081588|FD-Score:3.58|P-value:9.79E-5 ID:SGTC_1782|Compound:st039423|FD-Score:3.78|P-value:4.14E-5 ID:SGTC_1783|Compound:st039459|FD-Score:5.80|P-value:8.68E-10 ID:SGTC_1966|Compound:st070145|FD-Score:3.19|P-value:4.53E-4 ID:SGTC_2101|Compound:5377231|FD-Score:3.80|P-value:3.90E-5 ID:SGTC_2334|Compound:9017922|FD-Score:3.14|P-value:5.36E-4 ID:SGTC_2398|Compound:5228213|FD-Score:-3.19|P-value:5.05E-4 ID:SGTC_2405|Compound:ticlatone|FD-Score:-4.86|P-value:2.65E-7 ID:SGTC_2416|Compound:tofa|FD-Score:-3.53|P-value:1.39E-4 ID:SGTC_2536|Compound:dehydroabietamide|FD-Score:-3.64|P-value:8.84E-5 ID:SGTC_2822|Compound:7994718|FD-Score:3.82|P-value:3.58E-5 ID:SGTC_2923|Compound:7992654|FD-Score:3.15|P-value:5.18E-4 ID:SGTC_2974|Compound:9094440|FD-Score:-3.41|P-value:2.21E-4 ID:SGTC_2999|Compound:9069765|FD-Score:-3.45|P-value:1.88E-4 ID:SGTC_3136|Compound:9095672|FD-Score:-5.25|P-value:3.06E-8 ID:SGTC_3145|Compound:9085516|FD-Score:4.24|P-value:5.21E-6 ID:SGTC_3202|Compound:9113761|FD-Score:-3.39|P-value:2.43E-4 ID:SGTC_3277|Compound:9138724|FD-Score:-3.13|P-value:6.35E-4 ID:SGTC_3283|Compound:9109332|FD-Score:-3.85|P-value:3.59E-5 ID:SGTC_3284|Compound:9108171|FD-Score:-3.12|P-value:6.50E-4 ID:SGTC_3316|Compound:9136639|FD-Score:3.64|P-value:7.54E-5

Top fitness defect scores for YLR265C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1783 st039459 5.80 87.5 µM 8.68E-10 Golgi 5 3.38E-9
2 SGTC_644 1498-1197 5.14 63.3 µM 4.57E-8 4 1.36E-7
3 SGTC_1171 0368-0077 4.35 21.4 µM 3.09E-6 17 6.88E-6
4 SGTC_504 lovastatin 4.25 124.0 µM 5.03E-6 9 1.08E-5
5 SGTC_3145 9085516 4.24 49.5 µM 5.21E-6 fatty acid desaturase (OLE1) 20 1.12E-5
6 SGTC_501 nsc-95397 3.94 161.0 µM 2.11E-5 15 4.12E-5
7 SGTC_1149 3909-7961 3.85 23.3 µM 3.04E-5 21 5.79E-5
8 SGTC_2822 7994718 3.82 71.4 µM 3.58E-5 21 6.75E-5
9 SGTC_2101 5377231 3.80 179.2 µM 3.90E-5 10 7.31E-5
10 SGTC_1782 st039423 3.78 80.6 µM 4.14E-5 26 7.73E-5
11 SGTC_700 3453-2283 3.66 142.0 µM 7.12E-5 amide catabolism 33 1.28E-4
12 SGTC_3316 9136639 3.64 10.6 µM 7.54E-5 19 1.35E-4
13 SGTC_1775 st081588 3.58 35.2 µM 9.79E-5 60S ribosome export 34 1.72E-4
14 SGTC_725 1497-0963 3.53 19.0 µM 1.19E-4 copper-dependent oxidative stress 29 2.07E-4
15 SGTC_500 h-89 3.32 112.0 µM 2.79E-4 cell wall signaling 52 4.58E-4
16 SGTC_1966 st070145 3.19 16.1 µM 4.53E-4 50 7.20E-4
17 SGTC_798 1287-0182 3.16 476.0 µM 4.98E-4 56 7.86E-4
18 SGTC_2923 7992654 3.15 58.1 µM 5.18E-4 Golgi 60 8.16E-4
19 SGTC_2334 9017922 3.14 200.0 µM 5.36E-4 52 8.42E-4
20 SGTC_1404 3909-7820 3.06 48.3 µM 7.19E-4 60S ribosome export 32 0.00111

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.102 3.84E-9 YIR016W_p YIR016W_p hom Putative protein of unknown function; expression directly regulated by the metabolic and meiotic transcriptional regulator Ume6p; overexpression causes a cell cycle delay or arrest; non-essential gene; YIR016W has a paralog, YOL036W, that arose from the whole genome duplication
0.101 5.50E-9 YLR275W SMD2 het Core Sm protein Sm D2; part of heteroheptameric complex (with Smb1p, Smd1p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D2
0.099 1.06E-8 YJL050W MTR4 het ATP-dependent 3'-5' RNA helicase of the DExD/H family; involved in nuclear RNA processing and degradation both as a component of TRAMP complex and in TRAMP-independent processes; TRAMP unwinds RNA duplexes, with Mtr4p unwinding activity stimulated by Pap2p/Air2p but not dependent on ongoing polyadenylation; contains an arch domain, with two coiled-coil arms/stalks and a globular fist/KOW domain, which has RNA binding activity and is required for 5.8S rRNA processing
0.093 7.44E-8 YER060W-A FCY22 hom Putative purine-cytosine permease, very similar to Fcy2p but cannot substitute for its function
0.090 2.02E-7 YJR108W ABM1 hom Protein of unknown function, required for normal microtubule organization
0.087 4.60E-7 YIR028W DAL4 hom Allantoin permease; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation
0.086 6.52E-7 YNL181W_p YNL181W_p het Putative oxidoreductase; required for cell viability
0.086 6.60E-7 YKR063C LAS1 het Protein required for pre-rRNA processing at both ends of ITS2; may coordinate the action of the Rat1p-Rai1p exoRNAse; required for the G1/S transition of the cell cycle; human ortholog is Las1L; mutants require the SSD1-v allele for viability
0.081 2.52E-6 YBR024W SCO2 hom Protein anchored to mitochondrial inner membrane; may have a redundant function with Sco1p in delivery of copper to cytochrome c oxidase; interacts with Cox2p; SCO2 has a paralog, SCO1, that arose from the whole genome duplication
0.079 4.73E-6 YOR276W CAF20 hom Phosphoprotein of the mRNA cap-binding complex involved in translational control, repressor of cap-dependent translation initiation, competes with eIF4G for binding to eIF4E
0.079 5.10E-6 YHR091C MSR1 hom Mitochondrial arginyl-tRNA synthetase; mutations in human ortholog are associated with pontocerebellar hypoplasia type 6
0.077 8.41E-6 YDR104C SPO71 hom Meiosis-specific protein of unknown function, required for spore wall formation during sporulation, in particular for the proper size of the prospore membrane (PSM); interacts with SPO1; dispensable for both nuclear divisions during meiosis; contains two PH domains
0.074 1.92E-5 YER053C PIC2 hom Mitochondrial phosphate carrier, imports inorganic phosphate into mitochondria; functionally redundant with Mir1p but less abundant than Mir1p under normal conditions; expression is induced at high temperature
0.073 2.19E-5 YOR012W_p YOR012W_p hom Putative protein of unknown function
0.072 2.82E-5 YER073W ALD5 hom Mitochondrial aldehyde dehydrogenase, involved in regulation or biosynthesis of electron transport chain components and acetate formation; activated by K+; utilizes NADP+ as the preferred coenzyme; constitutively expressed