YLR276C / DBP9

DEAD-box protein required for 27S rRNA processing; exhibits DNA, RNA and DNA/RNA helicase activities; ATPase activity shows preference for DNA over RNA; DNA helicase activity abolished by mutation in RNA-binding domain

Zygosity: Heterozygous strain
fixedexpanded
Profile for YLR276C / DBP9

Click on Significant Values for Screen Details ID:SGTC_38|Compound:0929-0051|FD-Score:3.20|P-value:9.74E-5 ID:SGTC_39|Compound:0986-0283|FD-Score:6.40|P-value:4.28E-14 ID:SGTC_42|Compound:0133-0086|FD-Score:-3.25|P-value:6.66E-5 ID:SGTC_128|Compound:0906-2981|FD-Score:-4.14|P-value:6.00E-7 ID:SGTC_129|Compound:0088-0017|FD-Score:-2.67|P-value:8.41E-4 ID:SGTC_142|Compound:0335-0849|FD-Score:3.46|P-value:2.79E-5 ID:SGTC_144|Compound:4161-2749|FD-Score:-2.92|P-value:3.01E-4 ID:SGTC_242|Compound:5hr heat shock (37°C) + 5-fluorouracil|FD-Score:2.77|P-value:6.49E-4 ID:SGTC_610|Compound:4215-0184|FD-Score:3.77|P-value:5.67E-6 ID:SGTC_819|Compound:1013-0258|FD-Score:3.43|P-value:3.24E-5 ID:SGTC_909|Compound:3448-9333|FD-Score:-3.15|P-value:1.08E-4 ID:SGTC_1051|Compound:3253-3903|FD-Score:-2.79|P-value:5.22E-4 ID:SGTC_1073|Compound:triclabendazole|FD-Score:3.57|P-value:1.59E-5 ID:SGTC_1129|Compound:3453-1384|FD-Score:5.13|P-value:1.14E-9 ID:SGTC_1371|Compound:2421-0008|FD-Score:3.67|P-value:9.56E-6 ID:SGTC_1570|Compound:verbenone|FD-Score:3.89|P-value:2.93E-6 ID:SGTC_1605|Compound:st000082|FD-Score:-3.01|P-value:1.99E-4 ID:SGTC_2023|Compound:5144124|FD-Score:-3.72|P-value:6.08E-6 ID:SGTC_2150|Compound:5556613|FD-Score:3.88|P-value:3.12E-6 ID:SGTC_2439|Compound:5492030|FD-Score:4.36|P-value:1.92E-7 ID:SGTC_2495|Compound:cafestol|FD-Score:-3.58|P-value:1.28E-5 ID:SGTC_2504|Compound:purpurogallin|FD-Score:2.79|P-value:5.92E-4 ID:SGTC_2594|Compound:a-mangostin|FD-Score:3.22|P-value:8.96E-5 ID:SGTC_38|Compound:0929-0051|FD-Score:3.20|P-value:9.74E-5 ID:SGTC_39|Compound:0986-0283|FD-Score:6.40|P-value:4.28E-14 ID:SGTC_42|Compound:0133-0086|FD-Score:-3.25|P-value:6.66E-5 ID:SGTC_128|Compound:0906-2981|FD-Score:-4.14|P-value:6.00E-7 ID:SGTC_129|Compound:0088-0017|FD-Score:-2.67|P-value:8.41E-4 ID:SGTC_142|Compound:0335-0849|FD-Score:3.46|P-value:2.79E-5 ID:SGTC_144|Compound:4161-2749|FD-Score:-2.92|P-value:3.01E-4 ID:SGTC_242|Compound:5hr heat shock (37°C) + 5-fluorouracil|FD-Score:2.77|P-value:6.49E-4 ID:SGTC_610|Compound:4215-0184|FD-Score:3.77|P-value:5.67E-6 ID:SGTC_819|Compound:1013-0258|FD-Score:3.43|P-value:3.24E-5 ID:SGTC_909|Compound:3448-9333|FD-Score:-3.15|P-value:1.08E-4 ID:SGTC_1051|Compound:3253-3903|FD-Score:-2.79|P-value:5.22E-4 ID:SGTC_1073|Compound:triclabendazole|FD-Score:3.57|P-value:1.59E-5 ID:SGTC_1129|Compound:3453-1384|FD-Score:5.13|P-value:1.14E-9 ID:SGTC_1371|Compound:2421-0008|FD-Score:3.67|P-value:9.56E-6 ID:SGTC_1570|Compound:verbenone|FD-Score:3.89|P-value:2.93E-6 ID:SGTC_1605|Compound:st000082|FD-Score:-3.01|P-value:1.99E-4 ID:SGTC_2023|Compound:5144124|FD-Score:-3.72|P-value:6.08E-6 ID:SGTC_2150|Compound:5556613|FD-Score:3.88|P-value:3.12E-6 ID:SGTC_2439|Compound:5492030|FD-Score:4.36|P-value:1.92E-7 ID:SGTC_2495|Compound:cafestol|FD-Score:-3.58|P-value:1.28E-5 ID:SGTC_2504|Compound:purpurogallin|FD-Score:2.79|P-value:5.92E-4 ID:SGTC_2594|Compound:a-mangostin|FD-Score:3.22|P-value:8.96E-5

Top fitness defect scores for YLR276C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_39 0986-0283 6.40 58.4 µM 4.28E-14 calcium & mitochondrial duress 5 7.93E-11 1.41
2 SGTC_1129 3453-1384 5.13 223.0 µM 1.14E-9 8 1.47E-7 0.10
3 SGTC_2439 5492030 4.36 7.2 µM 1.92E-7 4 6.57E-6 0.34
4 SGTC_1570 verbenone 3.89 133.0 µM 2.93E-6 4 4.98E-5 0.12
5 SGTC_2150 5556613 3.88 200.0 µM 3.12E-6 4 5.22E-5 0.04
6 SGTC_610 4215-0184 3.77 155.0 µM 5.67E-6 exosome 26 8.15E-5 0.20
7 SGTC_1371 2421-0008 3.67 32.7 µM 9.56E-6 mitochondrial processes 30 1.20E-4 0.19
8 SGTC_1073 triclabendazole 3.57 3.1 µM 1.59E-5 1 1.76E-4 0.23
9 SGTC_142 0335-0849 3.46 62.7 µM 2.79E-5 ubiquinone biosynthesis & proteasome 26 2.68E-4 0.01
10 SGTC_819 1013-0258 3.43 27.7 µM 3.24E-5 7 2.99E-4 0.04
11 SGTC_2594 a-mangostin 3.22 100.0 µM 8.96E-5 18 6.40E-4 0.15
12 SGTC_38 0929-0051 3.20 58.3 µM 9.74E-5 13 6.81E-4 0.10
13 SGTC_2504 purpurogallin 2.79 52.3 µM 5.92E-4 RPP1 & pyrimidine depletion 13 0.00264 0.02
14 SGTC_242 5hr heat shock (37°C) + 5-fluorouracil 2.77 4.6 µM 6.49E-4 heat shock/prefoldin 45 0.00283 0.01
15 SGTC_3119 9125183 2.63 49.5 µM 0.00111 14 0.00423 0.01
16 SGTC_190 3970-0790 2.62 3.7 µM 0.00115 16 0.00435 0.01
17 SGTC_1913 st060213 2.62 96.1 µM 0.00115 17 0.00436 0.07
18 SGTC_1191 1487-1166 2.61 10.6 µM 0.00121 azole & statin 18 0.00453 0.05
19 SGTC_1370 2001-0092 2.58 44.6 µM 0.00136 25 0.00495 1.48E-4
20 SGTC_2390 9097792 2.57 176.6 µM 0.00143 19 0.00514 0.04

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.142 1.53E-16 YKL100C_p YKL100C_p hom Putative protein of unknown function with similarity to a human minor histocompatibility antigen and signal peptide peptidases; YKL100C is not an essential gene
0.132 1.60E-14 YJR011C_p YJR011C_p hom Putative protein of unknown function; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS
0.131 2.66E-14 YHR110W ERP5 hom Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport
0.130 5.01E-14 YML101C CUE4 hom Protein of unknown function; has a CUE domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination; CUE4 has a paralog, CUE1, that arose from the whole genome duplication
0.127 1.96E-13 YBR109C CMD1 het Calmodulin; Ca++ binding protein that regulates Ca++ independent processes (mitosis, bud growth, actin organization, endocytosis, etc.) and Ca++ dependent processes (stress-activated pathways), targets include Nuf1p, Myo2p and calcineurin
0.124 5.59E-13 YML116W ATR1 hom Multidrug efflux pump of the major facilitator superfamily; required for resistance to aminotriazole and 4-nitroquinoline-N-oxide; ATR1 has a paralog, YMR279C, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
0.123 7.01E-13 YMR210W YMR210W hom Putative acyltransferase with similarity to Eeb1p and Eht1p, has a minor role in medium-chain fatty acid ethyl ester biosynthesis; may be involved in lipid metabolism and detoxification
0.120 2.63E-12 YJR015W_p YJR015W_p hom Putative protein of unknown function; localizes to the endoplasmic reticulum and cytoplasm; predicted to encode a membrane transporter based on phylogenetic analysis; YJR015W is a non-essential gene
0.119 4.81E-12 YJL109C UTP10 het Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA; mutant has increased aneuploidy tolerance
0.117 1.13E-11 YLR003C CMS1 hom Putative subunit of the 90S preribosome processome complex; overexpression rescues supressor mutant of mcm10; null mutant is viable; relocalizes from nucleus to cytoplasm upon DNA replication stress
0.113 4.67E-11 YLR023C IZH3 hom Membrane protein involved in zinc ion homeostasis, member of the four-protein IZH family, expression induced by zinc deficiency; deletion reduces sensitivity to elevated zinc and shortens lag phase, overexpression reduces Zap1p activity
0.111 1.24E-10 YMR008C PLB1 hom Phospholipase B (lysophospholipase) involved in lipid metabolism, required for deacylation of phosphatidylcholine and phosphatidylethanolamine but not phosphatidylinositol
0.111 1.35E-10 YGR117C_p YGR117C_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.110 1.83E-10 YKR010C TOF2 hom Protein required for rDNA silencing and mitotic rDNA condensation; stimulates Cdc14p phosphatase activity and biphasic release to promote rDNA repeat segregation; required for condensin recruitment to the replication fork barrier site; TOF2 has a paralog, NET1, that arose from the whole genome duplication
0.105 1.01E-9 YGL260W_p YGL260W_p hom Putative protein of unknown function; transcription is significantly increased in a NAP1 deletion background; deletion mutant has increased accumulation of nickel and selenium