YLR288C / MEC3

DNA damage and meiotic pachytene checkpoint protein; subunit of a heterotrimeric complex (Rad17p-Mec3p-Ddc1p) that forms a sliding clamp, loaded onto partial duplex DNA by a clamp loader complex; homolog of human and S. pombe Hus1

Zygosity: Homozygous strain
fixedexpanded
Profile for YLR288C / MEC3

Click on Significant Values for Screen Details ID:SGTC_6|Compound:0844-0013|FD-Score:6.25|P-value:1.69E-12 ID:SGTC_35|Compound:0133-0054|FD-Score:-3.24|P-value:1.39E-4 ID:SGTC_42|Compound:0133-0086|FD-Score:-3.12|P-value:2.32E-4 ID:SGTC_349|Compound:k015-0014|FD-Score:-3.61|P-value:2.62E-5 ID:SGTC_669|Compound:0108-0021|FD-Score:3.52|P-value:4.58E-5 ID:SGTC_750|Compound:4073-0104|FD-Score:-6.03|P-value:7.41E-12 ID:SGTC_774|Compound:k060-0086|FD-Score:2.99|P-value:4.42E-4 ID:SGTC_790|Compound:1575-0012|FD-Score:3.01|P-value:4.11E-4 ID:SGTC_831|Compound:k061-1017|FD-Score:2.89|P-value:6.53E-4 ID:SGTC_900|Compound:1112-0045|FD-Score:2.86|P-value:7.43E-4 ID:SGTC_901|Compound:1112-0062|FD-Score:2.84|P-value:7.87E-4 ID:SGTC_1001|Compound:4048-4355|FD-Score:-3.22|P-value:1.53E-4 ID:SGTC_1011|Compound:4112-3381|FD-Score:3.01|P-value:4.05E-4 ID:SGTC_1033|Compound:k072-0102|FD-Score:2.90|P-value:6.23E-4 ID:SGTC_1034|Compound:k072-0165|FD-Score:-3.64|P-value:2.23E-5 ID:SGTC_1107|Compound:amlodipine|FD-Score:-6.46|P-value:2.49E-13 ID:SGTC_1254|Compound:0550-0035|FD-Score:-3.19|P-value:1.71E-4 ID:SGTC_1258|Compound:0671-0043|FD-Score:3.28|P-value:1.34E-4 ID:SGTC_1265|Compound:0819-1001|FD-Score:-2.88|P-value:6.20E-4 ID:SGTC_1350|Compound:1495-0454|FD-Score:3.10|P-value:2.84E-4 ID:SGTC_1355|Compound:1499-0035|FD-Score:-3.57|P-value:3.16E-5 ID:SGTC_1357|Compound:1503-1303|FD-Score:3.00|P-value:4.27E-4 ID:SGTC_1360|Compound:1598-0022|FD-Score:4.18|P-value:1.61E-6 ID:SGTC_1385|Compound:3091-4834|FD-Score:3.18|P-value:2.02E-4 ID:SGTC_1390|Compound:3413-2614|FD-Score:-3.19|P-value:1.72E-4 ID:SGTC_1459|Compound:k035-0031|FD-Score:-2.97|P-value:4.23E-4 ID:SGTC_1465|Compound:k213-0056|FD-Score:-3.17|P-value:1.90E-4 ID:SGTC_1574|Compound:spiramycin|FD-Score:-2.83|P-value:7.46E-4 ID:SGTC_1673|Compound:st016611|FD-Score:3.77|P-value:1.38E-5 ID:SGTC_1681|Compound:tripelennamine|FD-Score:-5.89|P-value:2.18E-11 ID:SGTC_1729|Compound:st035615|FD-Score:-3.35|P-value:8.55E-5 ID:SGTC_1735|Compound:st034310|FD-Score:2.93|P-value:5.61E-4 ID:SGTC_1776|Compound:st049249|FD-Score:-2.94|P-value:4.94E-4 ID:SGTC_1792|Compound:st045773|FD-Score:-2.87|P-value:6.48E-4 ID:SGTC_1889|Compound:st059080|FD-Score:3.39|P-value:7.99E-5 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:-3.00|P-value:3.78E-4 ID:SGTC_2017|Compound:5132927|FD-Score:5.72|P-value:9.46E-11 ID:SGTC_2038|Compound:4100038|FD-Score:3.86|P-value:8.64E-6 ID:SGTC_2053|Compound:5265679|FD-Score:-5.71|P-value:8.09E-11 ID:SGTC_2054|Compound:5233173|FD-Score:3.00|P-value:4.31E-4 ID:SGTC_2055|Compound:5235184|FD-Score:3.06|P-value:3.37E-4 ID:SGTC_2056|Compound:5235370|FD-Score:-3.15|P-value:2.06E-4 ID:SGTC_2114|Compound:5491910|FD-Score:3.06|P-value:3.37E-4 ID:SGTC_2128|Compound:5255449|FD-Score:-2.87|P-value:6.40E-4 ID:SGTC_2178|Compound:5812956|FD-Score:2.87|P-value:7.24E-4 ID:SGTC_2181|Compound:6316240|FD-Score:2.80|P-value:9.45E-4 ID:SGTC_2182|Compound:6325192|FD-Score:6.05|P-value:8.07E-12 ID:SGTC_2371|Compound:9071574|FD-Score:2.97|P-value:4.72E-4 ID:SGTC_2405|Compound:ticlatone|FD-Score:-3.64|P-value:2.24E-5 ID:SGTC_2423|Compound:st077119|FD-Score:-6.76|P-value:1.91E-14 ID:SGTC_2433|Compound:5607874|FD-Score:-3.76|P-value:1.24E-5 ID:SGTC_2492|Compound:derrusnin|FD-Score:3.47|P-value:5.65E-5 ID:SGTC_2493|Compound:resveratrol|FD-Score:3.62|P-value:2.77E-5 ID:SGTC_2494|Compound:18-aminoabieta-8,11,13-triene sulfate|FD-Score:7.73|P-value:3.35E-18 ID:SGTC_2501|Compound:sodium tetradecyl sulfate|FD-Score:4.26|P-value:1.06E-6 ID:SGTC_2503|Compound:beta-caryophyllene alcohol|FD-Score:3.72|P-value:1.71E-5 ID:SGTC_2553|Compound:4-methylesculetin|FD-Score:3.05|P-value:3.49E-4 ID:SGTC_2592|Compound:piperlongumine|FD-Score:4.04|P-value:3.44E-6 ID:SGTC_2610|Compound:ononetin|FD-Score:-2.88|P-value:6.08E-4 ID:SGTC_2680|Compound:butyl paraben|FD-Score:3.24|P-value:1.55E-4 ID:SGTC_2767|Compound:sibutramine|FD-Score:-2.87|P-value:6.40E-4 ID:SGTC_2906|Compound:7926292|FD-Score:-3.16|P-value:1.94E-4 ID:SGTC_2973|Compound:9094665|FD-Score:2.99|P-value:4.49E-4 ID:SGTC_3044|Compound:9093193|FD-Score:-3.07|P-value:2.91E-4 ID:SGTC_3324|Compound:9138759|FD-Score:2.87|P-value:7.07E-4 ID:SGTC_3351|Compound:9150312|FD-Score:-2.89|P-value:5.96E-4 ID:SGTC_6|Compound:0844-0013|FD-Score:6.25|P-value:1.69E-12 ID:SGTC_35|Compound:0133-0054|FD-Score:-3.24|P-value:1.39E-4 ID:SGTC_42|Compound:0133-0086|FD-Score:-3.12|P-value:2.32E-4 ID:SGTC_349|Compound:k015-0014|FD-Score:-3.61|P-value:2.62E-5 ID:SGTC_669|Compound:0108-0021|FD-Score:3.52|P-value:4.58E-5 ID:SGTC_750|Compound:4073-0104|FD-Score:-6.03|P-value:7.41E-12 ID:SGTC_774|Compound:k060-0086|FD-Score:2.99|P-value:4.42E-4 ID:SGTC_790|Compound:1575-0012|FD-Score:3.01|P-value:4.11E-4 ID:SGTC_831|Compound:k061-1017|FD-Score:2.89|P-value:6.53E-4 ID:SGTC_900|Compound:1112-0045|FD-Score:2.86|P-value:7.43E-4 ID:SGTC_901|Compound:1112-0062|FD-Score:2.84|P-value:7.87E-4 ID:SGTC_1001|Compound:4048-4355|FD-Score:-3.22|P-value:1.53E-4 ID:SGTC_1011|Compound:4112-3381|FD-Score:3.01|P-value:4.05E-4 ID:SGTC_1033|Compound:k072-0102|FD-Score:2.90|P-value:6.23E-4 ID:SGTC_1034|Compound:k072-0165|FD-Score:-3.64|P-value:2.23E-5 ID:SGTC_1107|Compound:amlodipine|FD-Score:-6.46|P-value:2.49E-13 ID:SGTC_1254|Compound:0550-0035|FD-Score:-3.19|P-value:1.71E-4 ID:SGTC_1258|Compound:0671-0043|FD-Score:3.28|P-value:1.34E-4 ID:SGTC_1265|Compound:0819-1001|FD-Score:-2.88|P-value:6.20E-4 ID:SGTC_1350|Compound:1495-0454|FD-Score:3.10|P-value:2.84E-4 ID:SGTC_1355|Compound:1499-0035|FD-Score:-3.57|P-value:3.16E-5 ID:SGTC_1357|Compound:1503-1303|FD-Score:3.00|P-value:4.27E-4 ID:SGTC_1360|Compound:1598-0022|FD-Score:4.18|P-value:1.61E-6 ID:SGTC_1385|Compound:3091-4834|FD-Score:3.18|P-value:2.02E-4 ID:SGTC_1390|Compound:3413-2614|FD-Score:-3.19|P-value:1.72E-4 ID:SGTC_1459|Compound:k035-0031|FD-Score:-2.97|P-value:4.23E-4 ID:SGTC_1465|Compound:k213-0056|FD-Score:-3.17|P-value:1.90E-4 ID:SGTC_1574|Compound:spiramycin|FD-Score:-2.83|P-value:7.46E-4 ID:SGTC_1673|Compound:st016611|FD-Score:3.77|P-value:1.38E-5 ID:SGTC_1681|Compound:tripelennamine|FD-Score:-5.89|P-value:2.18E-11 ID:SGTC_1729|Compound:st035615|FD-Score:-3.35|P-value:8.55E-5 ID:SGTC_1735|Compound:st034310|FD-Score:2.93|P-value:5.61E-4 ID:SGTC_1776|Compound:st049249|FD-Score:-2.94|P-value:4.94E-4 ID:SGTC_1792|Compound:st045773|FD-Score:-2.87|P-value:6.48E-4 ID:SGTC_1889|Compound:st059080|FD-Score:3.39|P-value:7.99E-5 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:-3.00|P-value:3.78E-4 ID:SGTC_2017|Compound:5132927|FD-Score:5.72|P-value:9.46E-11 ID:SGTC_2038|Compound:4100038|FD-Score:3.86|P-value:8.64E-6 ID:SGTC_2053|Compound:5265679|FD-Score:-5.71|P-value:8.09E-11 ID:SGTC_2054|Compound:5233173|FD-Score:3.00|P-value:4.31E-4 ID:SGTC_2055|Compound:5235184|FD-Score:3.06|P-value:3.37E-4 ID:SGTC_2056|Compound:5235370|FD-Score:-3.15|P-value:2.06E-4 ID:SGTC_2114|Compound:5491910|FD-Score:3.06|P-value:3.37E-4 ID:SGTC_2128|Compound:5255449|FD-Score:-2.87|P-value:6.40E-4 ID:SGTC_2178|Compound:5812956|FD-Score:2.87|P-value:7.24E-4 ID:SGTC_2181|Compound:6316240|FD-Score:2.80|P-value:9.45E-4 ID:SGTC_2182|Compound:6325192|FD-Score:6.05|P-value:8.07E-12 ID:SGTC_2371|Compound:9071574|FD-Score:2.97|P-value:4.72E-4 ID:SGTC_2405|Compound:ticlatone|FD-Score:-3.64|P-value:2.24E-5 ID:SGTC_2423|Compound:st077119|FD-Score:-6.76|P-value:1.91E-14 ID:SGTC_2433|Compound:5607874|FD-Score:-3.76|P-value:1.24E-5 ID:SGTC_2492|Compound:derrusnin|FD-Score:3.47|P-value:5.65E-5 ID:SGTC_2493|Compound:resveratrol|FD-Score:3.62|P-value:2.77E-5 ID:SGTC_2494|Compound:18-aminoabieta-8,11,13-triene sulfate|FD-Score:7.73|P-value:3.35E-18 ID:SGTC_2501|Compound:sodium tetradecyl sulfate|FD-Score:4.26|P-value:1.06E-6 ID:SGTC_2503|Compound:beta-caryophyllene alcohol|FD-Score:3.72|P-value:1.71E-5 ID:SGTC_2553|Compound:4-methylesculetin|FD-Score:3.05|P-value:3.49E-4 ID:SGTC_2592|Compound:piperlongumine|FD-Score:4.04|P-value:3.44E-6 ID:SGTC_2610|Compound:ononetin|FD-Score:-2.88|P-value:6.08E-4 ID:SGTC_2680|Compound:butyl paraben|FD-Score:3.24|P-value:1.55E-4 ID:SGTC_2767|Compound:sibutramine|FD-Score:-2.87|P-value:6.40E-4 ID:SGTC_2906|Compound:7926292|FD-Score:-3.16|P-value:1.94E-4 ID:SGTC_2973|Compound:9094665|FD-Score:2.99|P-value:4.49E-4 ID:SGTC_3044|Compound:9093193|FD-Score:-3.07|P-value:2.91E-4 ID:SGTC_3324|Compound:9138759|FD-Score:2.87|P-value:7.07E-4 ID:SGTC_3351|Compound:9150312|FD-Score:-2.89|P-value:5.96E-4

Top fitness defect scores for YLR288C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2494 18-aminoabieta-8,11,13-triene sulfate 7.73 3.7 µM 3.35E-18 3 5.30E-15
2 SGTC_6 0844-0013 6.25 19.8 µM 1.69E-12 11 2.10E-10
3 SGTC_2182 6325192 6.05 200.0 µM 8.07E-12 Golgi 3 7.41E-10
4 SGTC_2017 5132927 5.72 60.6 µM 9.46E-11 6 5.42E-9
5 SGTC_2501 sodium tetradecyl sulfate 4.26 13.4 µM 1.06E-6 26 1.02E-5
6 SGTC_1360 1598-0022 4.18 39.8 µM 1.61E-6 ERG2 24 1.44E-5
7 SGTC_2592 piperlongumine 4.04 83.3 µM 3.44E-6 TSC3-RPN4 36 2.67E-5
8 SGTC_2038 4100038 3.86 31.0 µM 8.64E-6 plasma membrane duress 55 5.64E-5
9 SGTC_1673 st016611 3.77 27.3 µM 1.38E-5 50 8.26E-5
10 SGTC_2503 beta-caryophyllene alcohol 3.72 24.7 µM 1.71E-5 14 9.82E-5
11 SGTC_2493 resveratrol 3.62 26.1 µM 2.77E-5 13 1.45E-4
12 SGTC_669 0108-0021 3.52 26.2 µM 4.58E-5 fatty acid desaturase (OLE1) 111 2.19E-4
13 SGTC_2492 derrusnin 3.47 100.0 µM 5.65E-5 10 2.60E-4
14 SGTC_1889 st059080 3.39 5.2 µM 7.99E-5 40 3.45E-4
15 SGTC_1258 0671-0043 3.28 57.1 µM 1.34E-4 55 5.25E-4
16 SGTC_2680 butyl paraben 3.24 22.2 µM 1.55E-4 amide catabolism 25 5.92E-4
17 SGTC_1385 3091-4834 3.18 14.1 µM 2.02E-4 RPP1 & pyrimidine depletion 43 7.34E-4
18 SGTC_1350 1495-0454 3.10 146.0 µM 2.84E-4 67 9.69E-4
19 SGTC_2114 5491910 3.06 81.2 µM 3.37E-4 46 0.00112
20 SGTC_2055 5235184 3.06 200.0 µM 3.37E-4 ERG2 74 0.00112

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.265 3.68E-55 YER067C-A_d YER067C-A_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YER067W
0.233 1.11E-42 YGR032W GSC2 hom Catalytic subunit of 1,3-beta-glucan synthase, involved in formation of the inner layer of the spore wall; activity positively regulated by Rho1p and negatively by Smk1p; has similarity to an alternate catalytic subunit, Fks1p (Gsc1p)
0.231 7.41E-42 YML053C_p YML053C_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; overexpression causes a cell cycle delay or arrest; YML053C is not an essential gene
0.222 8.60E-39 YGL017W ATE1 hom Arginyl-tRNA-protein transferase, catalyzes post-translational conjugation of arginine to the amino termini of acceptor proteins which are then subject to degradation via the N-end rule pathway
0.208 4.33E-34 YDL110C TMA17 hom Protein of unknown function that associates with ribosomes; heterozygous deletion demonstrated increases in chromosome instability in a rad9 deletion background; protein abundance is decreased upon intracellular iron depletion
0.194 6.69E-30 YDL049C KNH1 hom Protein with similarity to Kre9p, which is involved in cell wall beta 1,6-glucan synthesis; overproduction suppresses growth defects of a kre9 null mutant; required for propionic acid resistance
0.193 2.42E-29 YER060W-A FCY22 hom Putative purine-cytosine permease, very similar to Fcy2p but cannot substitute for its function
0.190 1.04E-28 YNL242W ATG2 hom Peripheral membrane protein required for autophagic vesicle formation; also required for vesicle formation during pexophagy and the cytoplasm-to-vaucole targeting (Cvt) pathway; involved in Atg9p cycling between the phagophore assembly site and mitochondria; forms cytoplasmic foci upon DNA replication stress
0.190 1.28E-28 YKL205W LOS1 hom Nuclear pore protein involved in nuclear export of pre-tRNA and in re-export of mature tRNAs after their retrograde import from the cytoplasm
0.190 1.63E-28 YEL049W PAU2 hom Member of the seripauperin multigene family encoded mainly in subtelomeric regions, active during alcoholic fermentation, regulated by anaerobiosis, negatively regulated by oxygen, repressed by heme
0.187 1.01E-27 YOR300W_d YOR300W_d hom Dubious open reading frame, unlikely to encode a protein; overlaps with verified gene BUD7/YOR299W; mutation affects bipolar budding and bud site selection, though phenotype could be due to the mutation's effects on BUD7
0.186 1.94E-27 YJR056C YJR056C hom Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; relative distribution to the nucleus increases upon DNA replication stress
0.184 8.07E-27 YLR026C SED5 het cis-Golgi t-SNARE syntaxin required for vesicular transport between the ER and the Golgi complex, binds at least 9 SNARE proteins
0.182 1.90E-26 YDR051C DET1 hom Acid phosphatase involved in the non-vesicular transport of sterols in both directions between the endoplasmic reticulum and plasma membrane; deletion confers sensitivity to nickel
0.177 4.54E-25 YKL131W_d YKL131W_d hom Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data