YLR306W / UBC12

Enzyme that mediates the conjugation of Rub1p, a ubiquitin-like protein, to other proteins; related to E2 ubiquitin-conjugating enzymes

Zygosity: Homozygous strain
fixedexpanded
Profile for YLR306W / UBC12

Click on Significant Values for Screen Details ID:SGTC_58|Compound:0957-0393|FD-Score:4.78|P-value:1.29E-7 ID:SGTC_87|Compound:0100-0052|FD-Score:3.34|P-value:1.58E-4 ID:SGTC_268|Compound:camptothecin|FD-Score:3.98|P-value:9.11E-6 ID:SGTC_497|Compound:capsazepine|FD-Score:-3.10|P-value:4.13E-4 ID:SGTC_760|Compound:0568-0725|FD-Score:3.50|P-value:8.09E-5 ID:SGTC_802|Compound:4112-3352|FD-Score:3.05|P-value:5.14E-4 ID:SGTC_896|Compound:0964-0007|FD-Score:3.68|P-value:3.60E-5 ID:SGTC_1023|Compound:4482-3888|FD-Score:2.96|P-value:7.20E-4 ID:SGTC_1025|Compound:k007-0731|FD-Score:-3.58|P-value:5.70E-5 ID:SGTC_1026|Compound:k007-1105|FD-Score:-3.25|P-value:2.30E-4 ID:SGTC_1029|Compound:k015-0030|FD-Score:3.69|P-value:3.52E-5 ID:SGTC_1042|Compound:0350-0096|FD-Score:2.98|P-value:6.69E-4 ID:SGTC_1072|Compound:oxiconazole|FD-Score:2.92|P-value:8.34E-4 ID:SGTC_1081|Compound:sertraline|FD-Score:-3.51|P-value:7.51E-5 ID:SGTC_1107|Compound:amlodipine|FD-Score:-4.84|P-value:9.08E-8 ID:SGTC_1300|Compound:1094-0077|FD-Score:2.91|P-value:8.62E-4 ID:SGTC_1373|Compound:2887-0003|FD-Score:3.09|P-value:4.36E-4 ID:SGTC_1745|Compound:st081495|FD-Score:3.25|P-value:2.36E-4 ID:SGTC_1869|Compound:3',7'-dimethoxyflavone|FD-Score:-3.52|P-value:7.22E-5 ID:SGTC_1870|Compound:st057514|FD-Score:5.92|P-value:9.03E-11 ID:SGTC_1880|Compound:MG-132|FD-Score:3.67|P-value:3.87E-5 ID:SGTC_1884|Compound:5633444|FD-Score:3.28|P-value:2.06E-4 ID:SGTC_2010|Compound:4012422|FD-Score:-3.94|P-value:1.07E-5 ID:SGTC_2011|Compound:4012611|FD-Score:-4.41|P-value:1.00E-6 ID:SGTC_2042|Compound:p-Dimethylaminoacetophenone|FD-Score:3.86|P-value:1.60E-5 ID:SGTC_2419|Compound:paclitaxel|FD-Score:4.93|P-value:5.48E-8 ID:SGTC_2502|Compound:avocadyne acetate|FD-Score:-3.25|P-value:2.32E-4 ID:SGTC_2504|Compound:purpurogallin|FD-Score:-2.91|P-value:8.50E-4 ID:SGTC_2572|Compound:astragaloside iv|FD-Score:2.92|P-value:8.31E-4 ID:SGTC_2586|Compound:curcumin|FD-Score:2.91|P-value:8.68E-4 ID:SGTC_2590|Compound:3,4',5',6',7'-pentamethoxyflavone|FD-Score:4.32|P-value:1.60E-6 ID:SGTC_2768|Compound:sulfasalazine|FD-Score:3.90|P-value:1.34E-5 ID:SGTC_2775|Compound:cyproheptadine|FD-Score:-3.12|P-value:3.84E-4 ID:SGTC_2821|Compound:7996764|FD-Score:3.17|P-value:3.13E-4 ID:SGTC_2831|Compound:7999036|FD-Score:3.47|P-value:9.37E-5 ID:SGTC_2835|Compound:9006588|FD-Score:-3.10|P-value:4.18E-4 ID:SGTC_2917|Compound:7979103|FD-Score:2.98|P-value:6.69E-4 ID:SGTC_2922|Compound:7999257|FD-Score:3.09|P-value:4.40E-4 ID:SGTC_2943|Compound:9068529|FD-Score:-4.09|P-value:5.21E-6 ID:SGTC_2948|Compound:9070189|FD-Score:3.53|P-value:7.27E-5 ID:SGTC_2950|Compound:9072895|FD-Score:-4.39|P-value:1.09E-6 ID:SGTC_2993|Compound:9054257|FD-Score:-3.41|P-value:1.16E-4 ID:SGTC_3055|Compound:9094636|FD-Score:3.08|P-value:4.58E-4 ID:SGTC_3221|Compound:9128209|FD-Score:3.05|P-value:5.03E-4 ID:SGTC_3266|Compound:9136860|FD-Score:3.33|P-value:1.69E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:4.78|P-value:1.29E-7 ID:SGTC_87|Compound:0100-0052|FD-Score:3.34|P-value:1.58E-4 ID:SGTC_268|Compound:camptothecin|FD-Score:3.98|P-value:9.11E-6 ID:SGTC_497|Compound:capsazepine|FD-Score:-3.10|P-value:4.13E-4 ID:SGTC_760|Compound:0568-0725|FD-Score:3.50|P-value:8.09E-5 ID:SGTC_802|Compound:4112-3352|FD-Score:3.05|P-value:5.14E-4 ID:SGTC_896|Compound:0964-0007|FD-Score:3.68|P-value:3.60E-5 ID:SGTC_1023|Compound:4482-3888|FD-Score:2.96|P-value:7.20E-4 ID:SGTC_1025|Compound:k007-0731|FD-Score:-3.58|P-value:5.70E-5 ID:SGTC_1026|Compound:k007-1105|FD-Score:-3.25|P-value:2.30E-4 ID:SGTC_1029|Compound:k015-0030|FD-Score:3.69|P-value:3.52E-5 ID:SGTC_1042|Compound:0350-0096|FD-Score:2.98|P-value:6.69E-4 ID:SGTC_1072|Compound:oxiconazole|FD-Score:2.92|P-value:8.34E-4 ID:SGTC_1081|Compound:sertraline|FD-Score:-3.51|P-value:7.51E-5 ID:SGTC_1107|Compound:amlodipine|FD-Score:-4.84|P-value:9.08E-8 ID:SGTC_1300|Compound:1094-0077|FD-Score:2.91|P-value:8.62E-4 ID:SGTC_1373|Compound:2887-0003|FD-Score:3.09|P-value:4.36E-4 ID:SGTC_1745|Compound:st081495|FD-Score:3.25|P-value:2.36E-4 ID:SGTC_1869|Compound:3',7'-dimethoxyflavone|FD-Score:-3.52|P-value:7.22E-5 ID:SGTC_1870|Compound:st057514|FD-Score:5.92|P-value:9.03E-11 ID:SGTC_1880|Compound:MG-132|FD-Score:3.67|P-value:3.87E-5 ID:SGTC_1884|Compound:5633444|FD-Score:3.28|P-value:2.06E-4 ID:SGTC_2010|Compound:4012422|FD-Score:-3.94|P-value:1.07E-5 ID:SGTC_2011|Compound:4012611|FD-Score:-4.41|P-value:1.00E-6 ID:SGTC_2042|Compound:p-Dimethylaminoacetophenone|FD-Score:3.86|P-value:1.60E-5 ID:SGTC_2419|Compound:paclitaxel|FD-Score:4.93|P-value:5.48E-8 ID:SGTC_2502|Compound:avocadyne acetate|FD-Score:-3.25|P-value:2.32E-4 ID:SGTC_2504|Compound:purpurogallin|FD-Score:-2.91|P-value:8.50E-4 ID:SGTC_2572|Compound:astragaloside iv|FD-Score:2.92|P-value:8.31E-4 ID:SGTC_2586|Compound:curcumin|FD-Score:2.91|P-value:8.68E-4 ID:SGTC_2590|Compound:3,4',5',6',7'-pentamethoxyflavone|FD-Score:4.32|P-value:1.60E-6 ID:SGTC_2768|Compound:sulfasalazine|FD-Score:3.90|P-value:1.34E-5 ID:SGTC_2775|Compound:cyproheptadine|FD-Score:-3.12|P-value:3.84E-4 ID:SGTC_2821|Compound:7996764|FD-Score:3.17|P-value:3.13E-4 ID:SGTC_2831|Compound:7999036|FD-Score:3.47|P-value:9.37E-5 ID:SGTC_2835|Compound:9006588|FD-Score:-3.10|P-value:4.18E-4 ID:SGTC_2917|Compound:7979103|FD-Score:2.98|P-value:6.69E-4 ID:SGTC_2922|Compound:7999257|FD-Score:3.09|P-value:4.40E-4 ID:SGTC_2943|Compound:9068529|FD-Score:-4.09|P-value:5.21E-6 ID:SGTC_2948|Compound:9070189|FD-Score:3.53|P-value:7.27E-5 ID:SGTC_2950|Compound:9072895|FD-Score:-4.39|P-value:1.09E-6 ID:SGTC_2993|Compound:9054257|FD-Score:-3.41|P-value:1.16E-4 ID:SGTC_3055|Compound:9094636|FD-Score:3.08|P-value:4.58E-4 ID:SGTC_3221|Compound:9128209|FD-Score:3.05|P-value:5.03E-4 ID:SGTC_3266|Compound:9136860|FD-Score:3.33|P-value:1.69E-4

Top fitness defect scores for YLR306W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature		Screen
Rank
Screen
P-value
1 SGTC_1870 st057514 5.92 26.5 µM 9.03E-11 RSC & ERG11 5 1.63E-9
2 SGTC_2419 paclitaxel 4.93 166.5 µM 5.48E-8 15 4.16E-7
3 SGTC_58 0957-0393 4.78 9.0 µM 1.29E-7 cell wall 308 8.75E-7
4 SGTC_2590 3,4',5',6',7'-pentamethoxyflavone 4.32 100.0 µM 1.60E-6 60S ribosome export 14 7.71E-6
5 SGTC_268 camptothecin 3.98 39.6 µM 9.11E-6 DNA damage response 70 3.49E-5
6 SGTC_2768 sulfasalazine 3.90 50.2 µM 1.34E-5 14 4.86E-5
7 SGTC_2042 p-Dimethylaminoacetophenone 3.86 200.0 µM 1.60E-5 19 5.67E-5
8 SGTC_1029 k015-0030 3.69 64.7 µM 3.52E-5 37 1.12E-4
9 SGTC_896 0964-0007 3.68 44.5 µM 3.60E-5 17 1.15E-4
10 SGTC_1880 MG-132 3.67 525.0 µM 3.87E-5 23 1.22E-4
11 SGTC_2948 9070189 3.53 9.4 µM 7.27E-5 plasma membrane duress 50 2.11E-4
12 SGTC_760 0568-0725 3.50 82.9 µM 8.09E-5 19 2.32E-4
13 SGTC_2831 7999036 3.47 19.5 µM 9.37E-5 70 2.63E-4
14 SGTC_87 0100-0052 3.34 35.5 µM 1.58E-4 169 4.15E-4
15 SGTC_3266 9136860 3.33 49.5 µM 1.69E-4 cell wall 66 4.40E-4
16 SGTC_1884 5633444 3.28 25.0 µM 2.06E-4 redox potentiating 86 5.21E-4
17 SGTC_1745 st081495 3.25 74.0 µM 2.36E-4 RNA processing & uracil transport 116 5.87E-4
18 SGTC_2821 7996764 3.17 71.4 µM 3.13E-4 78 7.51E-4
19 SGTC_1373 2887-0003 3.09 58.5 µM 4.36E-4 Golgi 52 0.00100
20 SGTC_2922 7999257 3.09 32.5 µM 4.40E-4 33 0.00101

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.158 3.29E-20 YKL076C_d PSY1_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 69% of ORF overlaps the uncharacterized ORF YKL075C
0.153 5.14E-19 YGR058W PEF1 hom Penta-EF-hand protein required for polar bud growth and cell wall abscission; binds calcium and zinc with different affinity; localizes to bud site in G1, bud neck in G2; binds to Sec31p and modulates COPII coat assembly
0.151 1.38E-18 YDR205W MSC2 hom Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Zrg17p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; localizes to ER and nucleus; mutations affect the cellular distribution of zinc and also confer defects in meiotic recombination between homologous chromatids
0.146 2.15E-17 YGR217W CCH1 hom Voltage-gated high-affinity calcium channel involved in calcium influx in response to some environmental stresses as well as exposure to mating pheromones; interacts and co-localizes with Mid1p, suggesting Cch1p and Mid1p function together
0.141 3.06E-16 YJR142W_p YJR142W_p hom Putative protein of unknown function; GST fusion protein is a Dbf2p-Mob1p phosphorylation target in a proteome chip analysis; synthetic lethal with PH085 deletion; plays a role in restricting Ty1 transposition; nudix hydrolase family member
0.140 4.58E-16 YGR025W_d YGR025W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.140 4.53E-16 YAL015C NTG1 hom DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase; involved in base excision repair; acts in both nucleus and mitochondrion; creates a double-strand break at mtDNA origins that stimulates replication in response to oxidative stress; NTG1 has a paralog, NTG2, that arose from the whole genome duplication
0.138 9.26E-16 YPR104C FHL1 het Regulator of ribosomal protein (RP) transcription; has forkhead associated domain that binds phosphorylated proteins; recruits coactivator Ifh1p or corepressor Crf1p to RP gene promoters; also has forkhead DNA-binding domain though in vitro DNA binding assays give inconsistent results; computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p motifs at others; suppresses RNA pol III and splicing factor prp4 mutants
0.138 1.04E-15 YGL009C LEU1 hom Isopropylmalate isomerase, catalyzes the second step in the leucine biosynthesis pathway
0.135 4.06E-15 YOR298C-A MBF1 hom Transcriptional coactivator; bridges the DNA-binding region of Gcn4p and TATA-binding protein Spt15p; suppressor of frameshift mutations; protein abundance increases in response to DNA replication stress
0.133 1.06E-14 YNR039C ZRG17 hom Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Msc2p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; zinc-regulated directly through Zap1p; transcription induced under conditions of zinc deficiency
0.128 9.72E-14 YNR007C ATG3 hom E2-like enzyme involved in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; plays a role in formation of Atg8p-phosphatidylethanolamine conjugates, which are involved in membrane dynamics during autophagy and Cvt; interaction with Atg8p is regulated by its acetylation by Esa1p (catalytic subunit of NuA4 histone acetyltransferase complex) while attenuation of Atg3 acetylation is mediated by histone deacetylase Rpd3p
0.127 1.37E-13 YGR032W GSC2 hom Catalytic subunit of 1,3-beta-glucan synthase, involved in formation of the inner layer of the spore wall; activity positively regulated by Rho1p and negatively by Smk1p; has similarity to an alternate catalytic subunit, Fks1p (Gsc1p)
0.127 1.89E-13 YBR120C CBP6 hom Mitochondrial protein required for translation of the COB mRNA; forms a complex with Cbp3p that binds to mt ribosomes near the polypeptide tunnel exit and promotes efficient translation of the COB mRNA; Cbp3p-Cbp6p complex also interacts with newly synthesized cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp into the cytochrome bc1 complex
0.127 2.01E-13 YMR223W UBP8 hom Ubiquitin-specific protease that is a component of the SAGA (Spt-Ada-Gcn5-Acetyltransferase) acetylation complex; required for SAGA-mediated deubiquitination of histone H2B