YLR342W / FKS1

Catalytic subunit of 1,3-beta-D-glucan synthase, functionally redundant with alternate catalytic subunit Gsc2p; binds to regulatory subunit Rho1p; involved in cell wall synthesis and maintenance; localizes to sites of cell wall remodeling

Zygosity: Homozygous strain
fixedexpanded
Profile for YLR342W / FKS1

Click on Significant Values for Screen Details ID:SGTC_26|Compound:0302-0163|FD-Score:3.31|P-value:5.16E-4 ID:SGTC_64|Compound:000l-0018|FD-Score:-3.90|P-value:5.36E-5 ID:SGTC_65|Compound:0798-0905|FD-Score:-3.44|P-value:3.14E-4 ID:SGTC_70|Compound:0141-0289|FD-Score:8.72|P-value:2.53E-18 ID:SGTC_73|Compound:0327-0325|FD-Score:-3.83|P-value:7.23E-5 ID:SGTC_119|Compound:0121-0043|FD-Score:3.68|P-value:1.30E-4 ID:SGTC_241|Compound:5hr heat shock (37°C) + cantharidin|FD-Score:-3.62|P-value:1.61E-4 ID:SGTC_313|Compound:3474-0040|FD-Score:3.65|P-value:1.45E-4 ID:SGTC_351|Compound:caspofungin|FD-Score:5.95|P-value:1.77E-9 ID:SGTC_363|Compound:0455-0021|FD-Score:3.92|P-value:5.02E-5 ID:SGTC_372|Compound:0155-0182|FD-Score:-4.35|P-value:7.98E-6 ID:SGTC_399|Compound:oxolinic acid|FD-Score:3.36|P-value:4.31E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:6.67|P-value:1.75E-11 ID:SGTC_455|Compound:paf C-16|FD-Score:4.84|P-value:7.73E-7 ID:SGTC_518|Compound:1021-0119|FD-Score:-3.18|P-value:7.91E-4 ID:SGTC_760|Compound:0568-0725|FD-Score:3.19|P-value:7.81E-4 ID:SGTC_798|Compound:1287-0182|FD-Score:-3.33|P-value:4.64E-4 ID:SGTC_819|Compound:1013-0258|FD-Score:3.43|P-value:3.34E-4 ID:SGTC_1104|Compound:tacrolimus|FD-Score:3.12|P-value:9.81E-4 ID:SGTC_1164|Compound:k060-0068|FD-Score:3.95|P-value:4.44E-5 ID:SGTC_1416|Compound:3966-0321|FD-Score:3.36|P-value:4.26E-4 ID:SGTC_1673|Compound:st016611|FD-Score:3.84|P-value:6.82E-5 ID:SGTC_1676|Compound:st018489|FD-Score:3.42|P-value:3.43E-4 ID:SGTC_1678|Compound:st019265|FD-Score:-3.31|P-value:5.02E-4 ID:SGTC_1719|Compound:st032227|FD-Score:5.16|P-value:1.53E-7 ID:SGTC_1788|Compound:fluconazole|FD-Score:3.55|P-value:2.12E-4 ID:SGTC_1803|Compound:st050068|FD-Score:3.93|P-value:4.73E-5 ID:SGTC_1863|Compound:st057641|FD-Score:5.75|P-value:5.62E-9 ID:SGTC_1873|Compound:rapamycin|FD-Score:7.64|P-value:1.70E-14 ID:SGTC_1874|Compound:staurosporine|FD-Score:5.59|P-value:1.41E-8 ID:SGTC_1927|Compound:st060202|FD-Score:-3.18|P-value:8.05E-4 ID:SGTC_1935|Compound:st074532|FD-Score:3.50|P-value:2.56E-4 ID:SGTC_1994|Compound:st072947|FD-Score:4.03|P-value:3.11E-5 ID:SGTC_2009|Compound:4012297|FD-Score:-3.27|P-value:5.73E-4 ID:SGTC_2277|Compound:7947813|FD-Score:-4.06|P-value:2.74E-5 ID:SGTC_2289|Compound:7946404|FD-Score:10.80|P-value:3.54E-27 ID:SGTC_2301|Compound:7769476|FD-Score:-3.40|P-value:3.71E-4 ID:SGTC_2387|Compound:9077560|FD-Score:3.31|P-value:5.17E-4 ID:SGTC_2401|Compound:5128683|FD-Score:4.49|P-value:4.12E-6 ID:SGTC_2542|Compound:2-hydroxy-5 (6)epoxy-tetrahydrocaryophyllene|FD-Score:3.23|P-value:6.67E-4 ID:SGTC_2579|Compound:2',5'-dihydroxy-4-methoxychalcone|FD-Score:3.93|P-value:4.78E-5 ID:SGTC_2617|Compound:dihydrotanshinone i|FD-Score:-6.08|P-value:7.69E-10 ID:SGTC_2619|Compound:obliquin|FD-Score:10.20|P-value:1.64E-24 ID:SGTC_2679|Compound:rapamycin|FD-Score:4.58|P-value:2.72E-6 ID:SGTC_2689|Compound:tacrolimus|FD-Score:5.70|P-value:7.85E-9 ID:SGTC_2719|Compound:novobiocin|FD-Score:-3.60|P-value:1.74E-4 ID:SGTC_2736|Compound:butenafine|FD-Score:-4.24|P-value:1.27E-5 ID:SGTC_2737|Compound:bleomycin|FD-Score:3.77|P-value:9.04E-5 ID:SGTC_2744|Compound:captopril|FD-Score:-3.40|P-value:3.72E-4 ID:SGTC_3120|Compound:9124077|FD-Score:3.89|P-value:5.60E-5 ID:SGTC_3143|Compound:9086658|FD-Score:3.33|P-value:4.74E-4 ID:SGTC_26|Compound:0302-0163|FD-Score:3.31|P-value:5.16E-4 ID:SGTC_64|Compound:000l-0018|FD-Score:-3.90|P-value:5.36E-5 ID:SGTC_65|Compound:0798-0905|FD-Score:-3.44|P-value:3.14E-4 ID:SGTC_70|Compound:0141-0289|FD-Score:8.72|P-value:2.53E-18 ID:SGTC_73|Compound:0327-0325|FD-Score:-3.83|P-value:7.23E-5 ID:SGTC_119|Compound:0121-0043|FD-Score:3.68|P-value:1.30E-4 ID:SGTC_241|Compound:5hr heat shock (37°C) + cantharidin|FD-Score:-3.62|P-value:1.61E-4 ID:SGTC_313|Compound:3474-0040|FD-Score:3.65|P-value:1.45E-4 ID:SGTC_351|Compound:caspofungin|FD-Score:5.95|P-value:1.77E-9 ID:SGTC_363|Compound:0455-0021|FD-Score:3.92|P-value:5.02E-5 ID:SGTC_372|Compound:0155-0182|FD-Score:-4.35|P-value:7.98E-6 ID:SGTC_399|Compound:oxolinic acid|FD-Score:3.36|P-value:4.31E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:6.67|P-value:1.75E-11 ID:SGTC_455|Compound:paf C-16|FD-Score:4.84|P-value:7.73E-7 ID:SGTC_518|Compound:1021-0119|FD-Score:-3.18|P-value:7.91E-4 ID:SGTC_760|Compound:0568-0725|FD-Score:3.19|P-value:7.81E-4 ID:SGTC_798|Compound:1287-0182|FD-Score:-3.33|P-value:4.64E-4 ID:SGTC_819|Compound:1013-0258|FD-Score:3.43|P-value:3.34E-4 ID:SGTC_1104|Compound:tacrolimus|FD-Score:3.12|P-value:9.81E-4 ID:SGTC_1164|Compound:k060-0068|FD-Score:3.95|P-value:4.44E-5 ID:SGTC_1416|Compound:3966-0321|FD-Score:3.36|P-value:4.26E-4 ID:SGTC_1673|Compound:st016611|FD-Score:3.84|P-value:6.82E-5 ID:SGTC_1676|Compound:st018489|FD-Score:3.42|P-value:3.43E-4 ID:SGTC_1678|Compound:st019265|FD-Score:-3.31|P-value:5.02E-4 ID:SGTC_1719|Compound:st032227|FD-Score:5.16|P-value:1.53E-7 ID:SGTC_1788|Compound:fluconazole|FD-Score:3.55|P-value:2.12E-4 ID:SGTC_1803|Compound:st050068|FD-Score:3.93|P-value:4.73E-5 ID:SGTC_1863|Compound:st057641|FD-Score:5.75|P-value:5.62E-9 ID:SGTC_1873|Compound:rapamycin|FD-Score:7.64|P-value:1.70E-14 ID:SGTC_1874|Compound:staurosporine|FD-Score:5.59|P-value:1.41E-8 ID:SGTC_1927|Compound:st060202|FD-Score:-3.18|P-value:8.05E-4 ID:SGTC_1935|Compound:st074532|FD-Score:3.50|P-value:2.56E-4 ID:SGTC_1994|Compound:st072947|FD-Score:4.03|P-value:3.11E-5 ID:SGTC_2009|Compound:4012297|FD-Score:-3.27|P-value:5.73E-4 ID:SGTC_2277|Compound:7947813|FD-Score:-4.06|P-value:2.74E-5 ID:SGTC_2289|Compound:7946404|FD-Score:10.80|P-value:3.54E-27 ID:SGTC_2301|Compound:7769476|FD-Score:-3.40|P-value:3.71E-4 ID:SGTC_2387|Compound:9077560|FD-Score:3.31|P-value:5.17E-4 ID:SGTC_2401|Compound:5128683|FD-Score:4.49|P-value:4.12E-6 ID:SGTC_2542|Compound:2-hydroxy-5 (6)epoxy-tetrahydrocaryophyllene|FD-Score:3.23|P-value:6.67E-4 ID:SGTC_2579|Compound:2',5'-dihydroxy-4-methoxychalcone|FD-Score:3.93|P-value:4.78E-5 ID:SGTC_2617|Compound:dihydrotanshinone i|FD-Score:-6.08|P-value:7.69E-10 ID:SGTC_2619|Compound:obliquin|FD-Score:10.20|P-value:1.64E-24 ID:SGTC_2679|Compound:rapamycin|FD-Score:4.58|P-value:2.72E-6 ID:SGTC_2689|Compound:tacrolimus|FD-Score:5.70|P-value:7.85E-9 ID:SGTC_2719|Compound:novobiocin|FD-Score:-3.60|P-value:1.74E-4 ID:SGTC_2736|Compound:butenafine|FD-Score:-4.24|P-value:1.27E-5 ID:SGTC_2737|Compound:bleomycin|FD-Score:3.77|P-value:9.04E-5 ID:SGTC_2744|Compound:captopril|FD-Score:-3.40|P-value:3.72E-4 ID:SGTC_3120|Compound:9124077|FD-Score:3.89|P-value:5.60E-5 ID:SGTC_3143|Compound:9086658|FD-Score:3.33|P-value:4.74E-4

Top fitness defect scores for YLR342W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2289 7946404 10.80 165.1 µM 3.54E-27 6 1.51E-27
2 SGTC_2619 obliquin 10.20 87.9 µM 1.64E-24 cell wall 20 7.67E-25
3 SGTC_70 0141-0289 8.72 43.3 µM 2.53E-18 mitochondrial stress 29 1.45E-18
4 SGTC_1873 rapamycin 7.64 2.0 nM 1.70E-14 13 1.11E-14
5 SGTC_453 enantio-paf c16 6.67 10.0 µM 1.75E-11 31 1.26E-11
6 SGTC_351 caspofungin 5.95 25.0 nM 1.77E-9 cell wall 42 1.36E-9
7 SGTC_1863 st057641 5.75 20.1 µM 5.62E-9 3 4.39E-9
8 SGTC_2689 tacrolimus 5.70 100.0 µM 7.85E-9 13 6.16E-9
9 SGTC_1874 staurosporine 5.59 7.6 µM 1.41E-8 cell wall signaling 14 1.12E-8
10 SGTC_1719 st032227 5.16 49.2 µM 1.53E-7 7 1.26E-7
11 SGTC_455 paf C-16 4.84 10.0 µM 7.73E-7 18 6.47E-7
12 SGTC_2679 rapamycin 4.58 1.0 nM 2.72E-6 112 2.32E-6
13 SGTC_2401 5128683 4.49 200.0 µM 4.12E-6 25 3.53E-6
14 SGTC_1994 st072947 4.03 40.9 µM 3.11E-5 15 2.74E-5
15 SGTC_1164 k060-0068 3.95 93.7 µM 4.44E-5 26 3.94E-5
16 SGTC_1803 st050068 3.93 52.4 µM 4.73E-5 NEO1-PIK1 183 4.19E-5
17 SGTC_2579 2',5'-dihydroxy-4-methoxychalcone 3.93 13.2 µM 4.78E-5 27 4.24E-5
18 SGTC_363 0455-0021 3.92 3.1 µM 5.02E-5 azole & statin 66 4.45E-5
19 SGTC_3120 9124077 3.89 49.5 µM 5.60E-5 25 4.98E-5
20 SGTC_1673 st016611 3.84 27.3 µM 6.82E-5 48 6.08E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.233 9.31E-43 YJL159W HSP150 hom O-mannosylated heat shock protein; secreted and covalently attached to the cell wall via beta-1,3-glucan and disulfide bridges; required for cell wall stability; induced by heat shock, oxidative stress, and nitrogen limitation; HSP150 has a paralog, PIR3, that arose from the whole genome duplication
0.198 4.67E-31 YML115C VAN1 hom Component of the mannan polymerase I, which contains Van1p and Mnn9p and is involved in the first steps of mannan synthesis; mutants are vanadate-resistant
0.195 3.62E-30 YDL117W CYK3 hom SH3-domain protein located in the bud neck and cytokinetic actin ring; relocalizes from bud neck to nucleus upon DNA replication stress; activates the chitin synthase activity of Chs2p during cytokinesis; suppressor of growth and cytokinesis defects of chs2 phospho-mutants
0.177 6.05E-25 YOR109W INP53 hom Polyphosphatidylinositol phosphatase, dephosphorylates multiple phosphatidylinositols; involved in trans Golgi network-to-early endosome pathway; hyperosmotic stress causes translocation to actin patches; contains Sac1 and 5-ptase domains
0.177 6.17E-25 YBR229C ROT2 hom Glucosidase II catalytic subunit required for normal cell wall synthesis; mutations in rot2 suppress tor2 mutations, and are synthetically lethal with rot1 mutations
0.170 3.06E-23 YHR155W YSP1 hom Mitochondrial protein; potential role in promoting mitochondrial fragmentation during programmed cell death in response to high levels of alpha-factor mating pheromone or the drug amiodarone; YSP1 has a paralog, SIP3, that arose from the whole genome duplication
0.165 6.45E-22 YLL040C VPS13 hom Protein of unknown function; heterooligomeric or homooligomeric complex; peripherally associated with membranes; involved in sporulation, vacuolar protein sorting, prospore membrane formation and protein-Golgi retention; homologous to human CHAC and COH1 which are involved in chorea acanthocytosis and Cohen syndrome, respectively
0.155 1.36E-19 YCL061C MRC1 hom S-phase checkpoint protein required for DNA replication; interacts with and stabilizes Pol2p at stalled replication forks during stress, where it forms a pausing complex with Tof1p and is phosphorylated by Mec1p; protects uncapped telomeres
0.150 2.15E-18 YLL021W SPA2 hom Component of the polarisome, which functions in actin cytoskeletal organization during polarized growth; acts as a scaffold for Mkk1p and Mpk1p cell wall integrity signaling components; potential Cdc28p substrate
0.150 2.93E-18 YPR061C JID1 hom Probable Hsp40p co-chaperone, has a DnaJ-like domain and appears to be involved in ER-associated degradation of misfolded proteins containing a tightly folded cytoplasmic domain; inhibits replication of Brome mosaic virus in S. cerevisiae
0.148 5.62E-18 YGL033W HOP2 hom Meiosis-specific protein that localizes to chromosomes, preventing synapsis between nonhomologous chromosomes and ensuring synapsis between homologs; complexes with Mnd1p to promote homolog pairing and meiotic double-strand break repair
0.148 6.33E-18 YDL192W ARF1 hom ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated vesicle formation in intracellular trafficking within the Golgi; ARF1 has a paralog, ARF2, that arose from the whole genome duplication
0.145 2.49E-17 YEL042W GDA1 hom Guanosine diphosphatase located in the Golgi, involved in the transport of GDP-mannose into the Golgi lumen by converting GDP to GMP after mannose is transferred its substrate
0.141 3.25E-16 YFL025C BST1 hom GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules
0.139 6.54E-16 YDL101C DUN1 hom Cell-cycle checkpoint serine-threonine kinase required for DNA damage-induced transcription of certain target genes, phosphorylation of Rad55p and Sml1p, and transient G2/M arrest after DNA damage; also regulates postreplicative DNA repair