HIPHOP chemogenomics database

YLR351C / NIT3

Nit protein, one of two proteins in S. cerevisiae with similarity to the Nit domain of NitFhit from fly and worm and to the mouse and human Nit protein which interacts with the Fhit tumor suppressor; nitrilase superfamily member

Zygosity: Homozygous strain

fixedexpanded
Profile for YLR351C / NIT3

Click on Significant Values for Screen Details

Top fitness defect scores for YLR351C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_291	4130-1278	5.13	35.5 µM	1.12E-8	Golgi	6	1.41E-7
2	SGTC_1240	0356-0016	4.61	42.1 µM	2.52E-7	mitochondrial response to ROS	23	1.97E-6
3	SGTC_2926	9010726	4.36	65.2 µM	1.04E-6		23	6.58E-6
4	SGTC_1214	0358-0031	4.07	63.7 µM	4.66E-6		13	2.34E-5
5	SGTC_3320	9137005	3.98	35.1 µM	7.41E-6	endomembrane recycling	15	3.47E-5
6	SGTC_2871	9038510	3.87	13.0 µM	1.28E-5		20	5.53E-5
7	SGTC_2758	dehydroepiandrosterone	3.81	69.3 µM	1.65E-5		41	6.85E-5
8	SGTC_3074	9116018	3.79	49.5 µM	1.87E-5	RNA processing & uracil transport	22	7.62E-5
9	SGTC_2799	7812892	3.66	81.8 µM	3.34E-5	superoxide	36	1.25E-4
10	SGTC_337	1315-0038	3.62	354.0 µM	3.99E-5	amide catabolism	33	1.45E-4
11	SGTC_2611	pyromycin	3.45	23.9 µM	8.75E-5		58	2.83E-4
12	SGTC_545	4218-0007	3.37	221.0 µM	1.23E-4		47	3.80E-4
13	SGTC_2126	5251174	3.25	33.9 µM	2.01E-4		67	5.77E-4
14	SGTC_2006	4011487	3.23	200.0 µM	2.23E-4	endomembrane recycling	121	6.28E-4
15	SGTC_1956	st077085	3.18	54.0 µM	2.73E-4		50	7.48E-4
16	SGTC_3338	9144300	3.16	39.6 µM	2.91E-4	Golgi	50	7.88E-4
17	SGTC_3109	9122853	3.13	49.5 µM	3.32E-4		29	8.82E-4
18	SGTC_2755	lomerizine	3.09	42.7 µM	3.78E-4		65	9.87E-4
19	SGTC_808	0711-0032	3.04	135.0 µM	4.66E-4		49	0.00118
20	SGTC_705	1124-0212	3.01	310.0 µM	5.21E-4		53	0.00130

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.123	8.25E-13	YOL079W_d	YOL079W_d	hom	Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.123	9.04E-13	YIL013C	PDR11	hom	ATP-binding cassette (ABC) transporter, multidrug transporter involved in multiple drug resistance; mediates sterol uptake when sterol biosynthesis is compromised; regulated by Pdr1p; required for anaerobic growth
0.119	5.01E-12	YNL290W	RFC3	het	Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon
0.100	5.99E-9	YFL036W	RPO41	hom	Mitochondrial RNA polymerase; single subunit enzyme similar to those of T3 and T7 bacteriophages; requires a specificity subunit encoded by MTF1 for promoter recognition; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation
0.100	6.21E-9	YNL083W	SAL1	hom	ADP/ATP transporter; member of the Ca2+-binding subfamily of mitochondrial carriers, with two EF-hand motifs; transport activity of either Sal1p or Pet9p is critical for viability; polymorphic in different S. cerevisiae strains
0.099	9.28E-9	YPL256C	CLN2	hom	G1 cyclin involved in regulation of the cell cycle; activates Cdc28p kinase to promote the G1 to S phase transition; late G1 specific expression depends on transcription factor complexes, MBF (Swi6p-Mbp1p) and SBF (Swi6p-Swi4p)
0.099	9.46E-9	YFL034C-A	RPL22B	hom	Ribosomal 60S subunit protein L22B; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22B has a paralog, RPL22A, that arose from the whole genome duplication
0.097	1.60E-8	YFR030W	MET10	hom	Subunit alpha of assimilatory sulfite reductase, which converts sulfite into sulfide
0.093	6.59E-8	YGL015C_p	YGL015C_p	hom	Putative protein of unknown function; null mutants accumulate cargo in the Golgi
0.091	1.26E-7	YOR267C	HRK1	hom	Protein kinase; implicated in activation of the plasma membrane H(+)-ATPase Pma1p in response to glucose metabolism; plays a role in ion homeostasis; protein abundance increases in response to DNA replication stress
0.090	1.93E-7	YBL094C_d	YBL094C_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized ORF YBL095W
0.089	2.09E-7	YFL049W	SWP82	hom	Member of the SWI/SNF chromatin remodeling complex; has an as yet unidentified role in the complex; has identifiable counterparts in closely related yeast species; abundantly expressed in many growth conditions; paralog of Npl6p; relocates to the cytosol under hypoxic conditions
0.089	2.10E-7	YNL318C	HXT14	hom	Protein with similarity to hexose transporter family members, expression is induced in low glucose and repressed in high glucose; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.085	7.46E-7	YOR101W	RAS1	hom	GTPase involved in G-protein signaling in adenylate cyclase activation; plays a role in cell proliferation; localized to the plasma membrane; homolog of mammalian RAS proto-oncogenes; relative distribution to the nucleus increases upon DNA replication stress
0.085	8.84E-7	YGL262W_p	YGL262W_p	hom	Putative protein of unknown function; null mutant displays elevated sensitivity to expression of a mutant huntingtin fragment or of alpha-synuclein; YGL262W is not an essential gene