YLR353W / BUD8

Protein involved in bud-site selection; diploid mutants display a unipolar budding pattern instead of the wild-type bipolar pattern, and bud at the proximal pole

Zygosity: Homozygous strain
fixedexpanded
Profile for YLR353W / BUD8

Click on Significant Values for Screen Details ID:SGTC_42|Compound:0133-0086|FD-Score:-2.98|P-value:4.22E-4 ID:SGTC_141|Compound:0443-0274|FD-Score:-3.17|P-value:1.93E-4 ID:SGTC_166|Compound:k072-0058|FD-Score:-3.18|P-value:1.79E-4 ID:SGTC_189|Compound:3970-0742|FD-Score:2.83|P-value:5.68E-4 ID:SGTC_300|Compound:r073-0011|FD-Score:-3.04|P-value:3.24E-4 ID:SGTC_699|Compound:0375-0592|FD-Score:3.08|P-value:1.97E-4 ID:SGTC_705|Compound:1124-0212|FD-Score:2.90|P-value:4.22E-4 ID:SGTC_750|Compound:4073-0104|FD-Score:-3.40|P-value:6.83E-5 ID:SGTC_996|Compound:3970-0799|FD-Score:3.26|P-value:8.94E-5 ID:SGTC_998|Compound:3996-0085|FD-Score:4.75|P-value:2.69E-8 ID:SGTC_1001|Compound:4048-4355|FD-Score:-4.72|P-value:5.60E-8 ID:SGTC_1107|Compound:amlodipine|FD-Score:-7.30|P-value:8.38E-17 ID:SGTC_1527|Compound:coumarin|FD-Score:2.90|P-value:4.32E-4 ID:SGTC_1561|Compound:5-methoxyflavone|FD-Score:2.97|P-value:3.14E-4 ID:SGTC_1808|Compound:st050063|FD-Score:3.00|P-value:2.85E-4 ID:SGTC_1910|Compound:st062352|FD-Score:-3.49|P-value:4.56E-5 ID:SGTC_1915|Compound:st067105|FD-Score:-3.26|P-value:1.27E-4 ID:SGTC_2093|Compound:5331342|FD-Score:3.05|P-value:2.29E-4 ID:SGTC_2097|Compound:5376186|FD-Score:3.93|P-value:3.32E-6 ID:SGTC_2103|Compound:5378138|FD-Score:4.07|P-value:1.57E-6 ID:SGTC_2292|Compound:7671323|FD-Score:5.12|P-value:2.32E-9 ID:SGTC_2309|Compound:7664088|FD-Score:6.02|P-value:3.13E-12 ID:SGTC_2310|Compound:7668677|FD-Score:4.95|P-value:7.25E-9 ID:SGTC_2337|Compound:9009472|FD-Score:3.72|P-value:9.80E-6 ID:SGTC_2374|Compound:9071832|FD-Score:2.77|P-value:7.05E-4 ID:SGTC_2430|Compound:5571206|FD-Score:2.74|P-value:8.05E-4 ID:SGTC_2584|Compound:androsterone|FD-Score:2.84|P-value:5.45E-4 ID:SGTC_2757|Compound:clomiphene|FD-Score:3.09|P-value:1.92E-4 ID:SGTC_2855|Compound:9016525|FD-Score:-2.87|P-value:6.51E-4 ID:SGTC_3018|Compound:9082602|FD-Score:2.73|P-value:8.40E-4 ID:SGTC_3039|Compound:9090270|FD-Score:3.26|P-value:9.08E-5 ID:SGTC_3323|Compound:9138564|FD-Score:3.10|P-value:1.81E-4 ID:SGTC_3336|Compound:9143619|FD-Score:2.72|P-value:8.68E-4 ID:SGTC_42|Compound:0133-0086|FD-Score:-2.98|P-value:4.22E-4 ID:SGTC_141|Compound:0443-0274|FD-Score:-3.17|P-value:1.93E-4 ID:SGTC_166|Compound:k072-0058|FD-Score:-3.18|P-value:1.79E-4 ID:SGTC_189|Compound:3970-0742|FD-Score:2.83|P-value:5.68E-4 ID:SGTC_300|Compound:r073-0011|FD-Score:-3.04|P-value:3.24E-4 ID:SGTC_699|Compound:0375-0592|FD-Score:3.08|P-value:1.97E-4 ID:SGTC_705|Compound:1124-0212|FD-Score:2.90|P-value:4.22E-4 ID:SGTC_750|Compound:4073-0104|FD-Score:-3.40|P-value:6.83E-5 ID:SGTC_996|Compound:3970-0799|FD-Score:3.26|P-value:8.94E-5 ID:SGTC_998|Compound:3996-0085|FD-Score:4.75|P-value:2.69E-8 ID:SGTC_1001|Compound:4048-4355|FD-Score:-4.72|P-value:5.60E-8 ID:SGTC_1107|Compound:amlodipine|FD-Score:-7.30|P-value:8.38E-17 ID:SGTC_1527|Compound:coumarin|FD-Score:2.90|P-value:4.32E-4 ID:SGTC_1561|Compound:5-methoxyflavone|FD-Score:2.97|P-value:3.14E-4 ID:SGTC_1808|Compound:st050063|FD-Score:3.00|P-value:2.85E-4 ID:SGTC_1910|Compound:st062352|FD-Score:-3.49|P-value:4.56E-5 ID:SGTC_1915|Compound:st067105|FD-Score:-3.26|P-value:1.27E-4 ID:SGTC_2093|Compound:5331342|FD-Score:3.05|P-value:2.29E-4 ID:SGTC_2097|Compound:5376186|FD-Score:3.93|P-value:3.32E-6 ID:SGTC_2103|Compound:5378138|FD-Score:4.07|P-value:1.57E-6 ID:SGTC_2292|Compound:7671323|FD-Score:5.12|P-value:2.32E-9 ID:SGTC_2309|Compound:7664088|FD-Score:6.02|P-value:3.13E-12 ID:SGTC_2310|Compound:7668677|FD-Score:4.95|P-value:7.25E-9 ID:SGTC_2337|Compound:9009472|FD-Score:3.72|P-value:9.80E-6 ID:SGTC_2374|Compound:9071832|FD-Score:2.77|P-value:7.05E-4 ID:SGTC_2430|Compound:5571206|FD-Score:2.74|P-value:8.05E-4 ID:SGTC_2584|Compound:androsterone|FD-Score:2.84|P-value:5.45E-4 ID:SGTC_2757|Compound:clomiphene|FD-Score:3.09|P-value:1.92E-4 ID:SGTC_2855|Compound:9016525|FD-Score:-2.87|P-value:6.51E-4 ID:SGTC_3018|Compound:9082602|FD-Score:2.73|P-value:8.40E-4 ID:SGTC_3039|Compound:9090270|FD-Score:3.26|P-value:9.08E-5 ID:SGTC_3323|Compound:9138564|FD-Score:3.10|P-value:1.81E-4 ID:SGTC_3336|Compound:9143619|FD-Score:2.72|P-value:8.68E-4

Top fitness defect scores for YLR353W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2309 7664088 6.02 163.9 µM 3.13E-12 5 8.86E-10
2 SGTC_2292 7671323 5.12 168.1 µM 2.32E-9 RNA processing & uracil transport 15 1.50E-7
3 SGTC_2310 7668677 4.95 200.0 µM 7.25E-9 14 3.62E-7
4 SGTC_998 3996-0085 4.75 177.0 µM 2.69E-8 8 1.00E-6
5 SGTC_2103 5378138 4.07 10.0 µM 1.57E-6 13 2.37E-5
6 SGTC_2097 5376186 3.93 200.0 µM 3.32E-6 20 4.24E-5
7 SGTC_2337 9009472 3.72 200.0 µM 9.80E-6 40 9.86E-5
8 SGTC_996 3970-0799 3.26 1.8 µM 8.94E-5 135 5.52E-4
9 SGTC_3039 9090270 3.26 49.5 µM 9.08E-5 RPP1 & pyrimidine depletion 26 5.59E-4
10 SGTC_3323 9138564 3.10 53.9 µM 1.81E-4 53 9.59E-4
11 SGTC_2757 clomiphene 3.09 4.2 µM 1.92E-4 fatty acid desaturase (OLE1) 86 0.00100
12 SGTC_699 0375-0592 3.08 111.0 µM 1.97E-4 iron homeostasis 57 0.00102
13 SGTC_2093 5331342 3.05 194.4 µM 2.29E-4 53 0.00115
14 SGTC_1808 st050063 3.00 61.5 µM 2.85E-4 48 0.00137
15 SGTC_1561 5-methoxyflavone 2.97 52.9 µM 3.14E-4 23 0.00147
16 SGTC_705 1124-0212 2.90 310.0 µM 4.22E-4 61 0.00185
17 SGTC_1527 coumarin 2.90 137.0 µM 4.32E-4 66 0.00189
18 SGTC_2584 androsterone 2.84 48.7 µM 5.45E-4 cell wall 230 0.00227
19 SGTC_189 3970-0742 2.83 2.1 µM 5.68E-4 35 0.00234
20 SGTC_2374 9071832 2.77 200.0 µM 7.05E-4 endomembrane recycling 49 0.00277

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.322 6.21E-82 YKL131W_d YKL131W_d hom Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.282 1.84E-62 YFL063W_d YFL063W_d hom Dubious open reading frame, based on available experimental and comparative sequence data
0.275 4.14E-59 YKL220C FRE2 hom Ferric reductase and cupric reductase, reduces siderophore-bound iron and oxidized copper prior to uptake by transporters; expression induced by low iron levels but not by low copper levels
0.256 1.54E-51 YOR037W CYC2 hom Mitochondrial peripheral inner membrane protein, contains a FAD cofactor in a domain exposed in the intermembrane space; exhibits redox activity in vitro; likely participates in ligation of heme to acytochromes c and c1 (Cyc1p and Cyt1p)
0.255 4.67E-51 YKR023W_p YKR023W_p hom Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.243 4.61E-46 YDL110C TMA17 hom Protein of unknown function that associates with ribosomes; heterozygous deletion demonstrated increases in chromosome instability in a rad9 deletion background; protein abundance is decreased upon intracellular iron depletion
0.239 8.39E-45 YOR298C-A MBF1 hom Transcriptional coactivator; bridges the DNA-binding region of Gcn4p and TATA-binding protein Spt15p; suppressor of frameshift mutations; protein abundance increases in response to DNA replication stress
0.236 9.44E-44 YBR262C AIM5 hom Mitochondrial inner membrane protein; subunit of the mitochondrial inner membrane organizing system (MitOS, MICOS, or MINOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture
0.234 7.51E-43 YBR207W FTH1 hom Putative high affinity iron transporter; involved in transport of intravacuolar stores of iron; forms complex with Fet5p; expression is regulated by iron; proposed to play indirect role in endocytosis; protein abundance increases in response to DNA replication stress
0.228 6.79E-41 YKL179C COY1 hom Golgi membrane protein with similarity to mammalian CASP; genetic interactions with GOS1 (encoding a Golgi snare protein) suggest a role in Golgi function
0.226 2.89E-40 YBL015W ACH1 hom Protein with CoA transferase activity, particularly for CoASH transfer from succinyl-CoA to acetate; has minor acetyl-CoA-hydrolase activity; phosphorylated; required for acetate utilization and for diploid pseudohyphal growth
0.223 3.20E-39 YPR010C RPA135 het RNA polymerase I second largest subunit A135
0.223 5.37E-39 YBL036C YBL036C hom Putative non-specific single-domain racemase based on structural similarity; binds pyridoxal 5'-phosphate; expression of GFP-fusion protein induced in response to the DNA-damaging agent MMS
0.219 1.22E-37 YAL060W BDH1 hom NAD-dependent (R,R)-butanediol dehydrogenase, catalyzes oxidation of (R,R)-2,3-butanediol to (3R)-acetoin, oxidation of meso-butanediol to (3S)-acetoin, and reduction of acetoin; enhances use of 2,3-butanediol as an aerobic carbon source
0.218 2.56E-37 YMR139W RIM11 hom Protein kinase; required for signal transduction during entry into meiosis; promotes the formation of the Ime1p-Ume6p complex by phosphorylating Ime1p and Ume6p; shares similarity with mammalian glycogen synthase kinase 3-beta; protein abundance increases in response to DNA replication stress