YLR360W / VPS38

Part of a Vps34p phosphatidylinositol 3-kinase complex that functions in carboxypeptidase Y (CPY) sorting; binds Vps30p and Vps34p to promote production of phosphatidylinositol 3-phosphate (PtdIns3P) which stimulates kinase activity; required for overflow degradation of misfolded proteins when ERAD is saturated

Zygosity: Homozygous strain
fixedexpanded
Profile for YLR360W / VPS38

Click on Significant Values for Screen Details ID:SGTC_42|Compound:0133-0086|FD-Score:-4.78|P-value:4.25E-7 ID:SGTC_58|Compound:0957-0393|FD-Score:-5.28|P-value:2.74E-8 ID:SGTC_206|Compound:4546-0025|FD-Score:8.24|P-value:6.89E-18 ID:SGTC_221|Compound:trifluoperazine|FD-Score:4.04|P-value:1.37E-5 ID:SGTC_301|Compound:4119-0100|FD-Score:6.86|P-value:5.71E-13 ID:SGTC_308|Compound:4092-1169|FD-Score:4.07|P-value:1.18E-5 ID:SGTC_309|Compound:k408-0051|FD-Score:-3.31|P-value:3.35E-4 ID:SGTC_377|Compound:3970-0964|FD-Score:5.12|P-value:5.38E-8 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-4.96|P-value:1.69E-7 ID:SGTC_459|Compound:nigericin|FD-Score:4.04|P-value:1.34E-5 ID:SGTC_525|Compound:k781-2675|FD-Score:3.95|P-value:2.03E-5 ID:SGTC_550|Compound:4123-0385|FD-Score:3.29|P-value:3.10E-4 ID:SGTC_923|Compound:1900-2434|FD-Score:-3.09|P-value:7.61E-4 ID:SGTC_943|Compound:3253-1379|FD-Score:-3.03|P-value:9.33E-4 ID:SGTC_946|Compound:3434-1334|FD-Score:-3.11|P-value:7.03E-4 ID:SGTC_1100|Compound:amiodarone|FD-Score:3.81|P-value:3.72E-5 ID:SGTC_1107|Compound:amlodipine|FD-Score:-7.88|P-value:2.29E-16 ID:SGTC_1118|Compound:chelidonine|FD-Score:3.79|P-value:4.03E-5 ID:SGTC_1120|Compound:ficusin|FD-Score:-3.30|P-value:3.55E-4 ID:SGTC_1268|Compound:0828-0275|FD-Score:3.80|P-value:3.96E-5 ID:SGTC_1308|Compound:1187-1617|FD-Score:4.12|P-value:9.22E-6 ID:SGTC_1587|Compound:estradiol valerate|FD-Score:3.28|P-value:3.28E-4 ID:SGTC_1754|Compound:st042065|FD-Score:3.56|P-value:1.08E-4 ID:SGTC_1800|Compound:st050079|FD-Score:5.38|P-value:1.18E-8 ID:SGTC_1803|Compound:st050068|FD-Score:6.39|P-value:1.76E-11 ID:SGTC_1818|Compound:st052136|FD-Score:4.21|P-value:6.03E-6 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:-3.71|P-value:6.81E-5 ID:SGTC_2015|Compound:5119600|FD-Score:-3.53|P-value:1.42E-4 ID:SGTC_2021|Compound:4019055|FD-Score:4.62|P-value:8.11E-7 ID:SGTC_2322|Compound:7413319|FD-Score:4.10|P-value:1.01E-5 ID:SGTC_2396|Compound:5342950|FD-Score:-3.49|P-value:1.66E-4 ID:SGTC_2399|Compound:5884133|FD-Score:-4.06|P-value:1.52E-5 ID:SGTC_2400|Compound:5203494|FD-Score:-5.61|P-value:3.86E-9 ID:SGTC_2429|Compound:5902844|FD-Score:3.60|P-value:9.20E-5 ID:SGTC_2438|Compound:5259590|FD-Score:3.56|P-value:1.08E-4 ID:SGTC_2442|Compound:5158906|FD-Score:3.13|P-value:5.56E-4 ID:SGTC_2458|Compound:5808907|FD-Score:3.04|P-value:7.75E-4 ID:SGTC_2538|Compound:3',7'-epoxycaryophyllan-6-one|FD-Score:-3.83|P-value:4.04E-5 ID:SGTC_2561|Compound:acetosyringone|FD-Score:-3.31|P-value:3.33E-4 ID:SGTC_2581|Compound:avocatin a|FD-Score:-3.07|P-value:8.16E-4 ID:SGTC_2591|Compound:skatole|FD-Score:3.19|P-value:4.46E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:4.42|P-value:2.22E-6 ID:SGTC_2699|Compound:st077704|FD-Score:4.39|P-value:2.56E-6 ID:SGTC_2704|Compound:st078022|FD-Score:2.99|P-value:9.32E-4 ID:SGTC_2709|Compound:st078560|FD-Score:3.03|P-value:8.01E-4 ID:SGTC_2836|Compound:9010338|FD-Score:-3.71|P-value:6.84E-5 ID:SGTC_2874|Compound:9043904|FD-Score:-3.11|P-value:7.02E-4 ID:SGTC_2964|Compound:9083345|FD-Score:-3.11|P-value:7.06E-4 ID:SGTC_2991|Compound:9074669|FD-Score:6.60|P-value:3.96E-12 ID:SGTC_3037|Compound:9091026|FD-Score:3.52|P-value:1.25E-4 ID:SGTC_3126|Compound:9127922|FD-Score:3.96|P-value:1.93E-5 ID:SGTC_3160|Compound:9100216|FD-Score:3.33|P-value:2.72E-4 ID:SGTC_3196|Compound:9111891|FD-Score:-4.01|P-value:1.84E-5 ID:SGTC_3199|Compound:9111318|FD-Score:-3.66|P-value:8.53E-5 ID:SGTC_3323|Compound:9138564|FD-Score:3.60|P-value:9.00E-5 ID:SGTC_42|Compound:0133-0086|FD-Score:-4.78|P-value:4.25E-7 ID:SGTC_58|Compound:0957-0393|FD-Score:-5.28|P-value:2.74E-8 ID:SGTC_206|Compound:4546-0025|FD-Score:8.24|P-value:6.89E-18 ID:SGTC_221|Compound:trifluoperazine|FD-Score:4.04|P-value:1.37E-5 ID:SGTC_301|Compound:4119-0100|FD-Score:6.86|P-value:5.71E-13 ID:SGTC_308|Compound:4092-1169|FD-Score:4.07|P-value:1.18E-5 ID:SGTC_309|Compound:k408-0051|FD-Score:-3.31|P-value:3.35E-4 ID:SGTC_377|Compound:3970-0964|FD-Score:5.12|P-value:5.38E-8 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-4.96|P-value:1.69E-7 ID:SGTC_459|Compound:nigericin|FD-Score:4.04|P-value:1.34E-5 ID:SGTC_525|Compound:k781-2675|FD-Score:3.95|P-value:2.03E-5 ID:SGTC_550|Compound:4123-0385|FD-Score:3.29|P-value:3.10E-4 ID:SGTC_923|Compound:1900-2434|FD-Score:-3.09|P-value:7.61E-4 ID:SGTC_943|Compound:3253-1379|FD-Score:-3.03|P-value:9.33E-4 ID:SGTC_946|Compound:3434-1334|FD-Score:-3.11|P-value:7.03E-4 ID:SGTC_1100|Compound:amiodarone|FD-Score:3.81|P-value:3.72E-5 ID:SGTC_1107|Compound:amlodipine|FD-Score:-7.88|P-value:2.29E-16 ID:SGTC_1118|Compound:chelidonine|FD-Score:3.79|P-value:4.03E-5 ID:SGTC_1120|Compound:ficusin|FD-Score:-3.30|P-value:3.55E-4 ID:SGTC_1268|Compound:0828-0275|FD-Score:3.80|P-value:3.96E-5 ID:SGTC_1308|Compound:1187-1617|FD-Score:4.12|P-value:9.22E-6 ID:SGTC_1587|Compound:estradiol valerate|FD-Score:3.28|P-value:3.28E-4 ID:SGTC_1754|Compound:st042065|FD-Score:3.56|P-value:1.08E-4 ID:SGTC_1800|Compound:st050079|FD-Score:5.38|P-value:1.18E-8 ID:SGTC_1803|Compound:st050068|FD-Score:6.39|P-value:1.76E-11 ID:SGTC_1818|Compound:st052136|FD-Score:4.21|P-value:6.03E-6 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:-3.71|P-value:6.81E-5 ID:SGTC_2015|Compound:5119600|FD-Score:-3.53|P-value:1.42E-4 ID:SGTC_2021|Compound:4019055|FD-Score:4.62|P-value:8.11E-7 ID:SGTC_2322|Compound:7413319|FD-Score:4.10|P-value:1.01E-5 ID:SGTC_2396|Compound:5342950|FD-Score:-3.49|P-value:1.66E-4 ID:SGTC_2399|Compound:5884133|FD-Score:-4.06|P-value:1.52E-5 ID:SGTC_2400|Compound:5203494|FD-Score:-5.61|P-value:3.86E-9 ID:SGTC_2429|Compound:5902844|FD-Score:3.60|P-value:9.20E-5 ID:SGTC_2438|Compound:5259590|FD-Score:3.56|P-value:1.08E-4 ID:SGTC_2442|Compound:5158906|FD-Score:3.13|P-value:5.56E-4 ID:SGTC_2458|Compound:5808907|FD-Score:3.04|P-value:7.75E-4 ID:SGTC_2538|Compound:3',7'-epoxycaryophyllan-6-one|FD-Score:-3.83|P-value:4.04E-5 ID:SGTC_2561|Compound:acetosyringone|FD-Score:-3.31|P-value:3.33E-4 ID:SGTC_2581|Compound:avocatin a|FD-Score:-3.07|P-value:8.16E-4 ID:SGTC_2591|Compound:skatole|FD-Score:3.19|P-value:4.46E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:4.42|P-value:2.22E-6 ID:SGTC_2699|Compound:st077704|FD-Score:4.39|P-value:2.56E-6 ID:SGTC_2704|Compound:st078022|FD-Score:2.99|P-value:9.32E-4 ID:SGTC_2709|Compound:st078560|FD-Score:3.03|P-value:8.01E-4 ID:SGTC_2836|Compound:9010338|FD-Score:-3.71|P-value:6.84E-5 ID:SGTC_2874|Compound:9043904|FD-Score:-3.11|P-value:7.02E-4 ID:SGTC_2964|Compound:9083345|FD-Score:-3.11|P-value:7.06E-4 ID:SGTC_2991|Compound:9074669|FD-Score:6.60|P-value:3.96E-12 ID:SGTC_3037|Compound:9091026|FD-Score:3.52|P-value:1.25E-4 ID:SGTC_3126|Compound:9127922|FD-Score:3.96|P-value:1.93E-5 ID:SGTC_3160|Compound:9100216|FD-Score:3.33|P-value:2.72E-4 ID:SGTC_3196|Compound:9111891|FD-Score:-4.01|P-value:1.84E-5 ID:SGTC_3199|Compound:9111318|FD-Score:-3.66|P-value:8.53E-5 ID:SGTC_3323|Compound:9138564|FD-Score:3.60|P-value:9.00E-5

Top fitness defect scores for YLR360W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_206 4546-0025 8.24 72.0 µM 6.89E-18 NEO1 20 8.60E-17
2 SGTC_301 4119-0100 6.86 24.5 µM 5.71E-13 NEO1 32 3.40E-12
3 SGTC_2991 9074669 6.60 71.4 µM 3.96E-12 azole & statin 14 2.08E-11
4 SGTC_1803 st050068 6.39 52.4 µM 1.76E-11 NEO1-PIK1 69 8.41E-11
5 SGTC_1800 st050079 5.38 29.1 µM 1.18E-8 NEO1-PIK1 61 3.67E-8
6 SGTC_377 3970-0964 5.12 33.1 µM 5.38E-8 27 1.52E-7
7 SGTC_2021 4019055 4.62 173.0 µM 8.11E-7 endomembrane recycling 83 1.92E-6
8 SGTC_2688 kanamycin b 4.42 96.7 µM 2.22E-6 calcium & mitochondrial duress 302 4.92E-6
9 SGTC_2699 st077704 4.39 54.6 µM 2.56E-6 endomembrane recycling 42 5.62E-6
10 SGTC_1818 st052136 4.21 56.3 µM 6.03E-6 NEO1-PIK1 39 1.25E-5
11 SGTC_1308 1187-1617 4.12 41.4 µM 9.22E-6 fatty acid desaturase (OLE1) 36 1.86E-5
12 SGTC_2322 7413319 4.10 38.2 µM 1.01E-5 ERAD & cell cycle 145 2.02E-5
13 SGTC_308 4092-1169 4.07 104.0 µM 1.18E-5 plasma membrane duress 47 2.35E-5
14 SGTC_459 nigericin 4.04 15.3 µM 1.34E-5 62 2.64E-5
15 SGTC_221 trifluoperazine 4.04 10.3 µM 1.37E-5 NEO1-PIK1 203 2.69E-5
16 SGTC_3126 9127922 3.96 49.5 µM 1.93E-5 fatty acid desaturase (OLE1) 33 3.71E-5
17 SGTC_525 k781-2675 3.95 26.7 µM 2.03E-5 redox potentiating 54 3.89E-5
18 SGTC_1100 amiodarone 3.81 5.1 µM 3.72E-5 18 6.86E-5
19 SGTC_1268 0828-0275 3.80 2.5 µM 3.96E-5 24 7.28E-5
20 SGTC_1118 chelidonine 3.79 707.5 nM 4.03E-5 18 7.40E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.340 2.17E-91 YJL053W PEP8 hom Vacuolar protein component of the retromer; forms part of the multimeric membrane-associated retromer complex involved in vacuolar protein sorting along with Vps35p, Vps29p, Vps17p, and Vps5p; essential for endosome-to-Golgi retrograde protein transport; interacts with Ypt7p; protein abundance increases in response to DNA replication stress
0.320 7.41E-81 YHR012W VPS29 hom Endosomal protein that is a subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde transport; forms a subcomplex with Vps35p and Vps26p that selects cargo proteins for endosome-to-Golgi retrieval
0.299 2.43E-70 YPL120W VPS30 hom Subunit of phosphatidylinositol (PtdIns) 3-kinase complexes I and II; Complex I is essential in autophagy and Complex II is required for vacuolar protein sorting; required for overflow degradation of misfolded proteins when ERAD is saturated; ortholog of the higher eukaryotic gene Beclin 1
0.286 2.14E-64 YJL154C VPS35 hom Endosomal subunit of membrane-associated retromer complex required for retrograde transport; receptor that recognizes retrieval signals on cargo proteins, forms subcomplex with Vps26p and Vps29p that selects cargo proteins for retrieval; interacts with Ypt7p
0.255 5.23E-51 YOR357C SNX3 hom Sorting nexin required to maintain late-Golgi resident enzymes in their proper location by recycling molecules from the prevacuolar compartment; contains a PX domain and sequence similarity to human Snx3p
0.248 4.02E-48 YNL294C RIM21 hom pH sensor molecule, component of the RIM101 pathway; has a role in cell wall construction and alkaline pH response; is glycosylated and phosphorylated; interacts with Dfg16p and Rim9p to form a pH-sensing complex; localization to the plasma membrane is dependent on Dfg16p and Rim9p; has similarity to A. nidulans PalH
0.247 7.63E-48 YDR317W HIM1 hom Protein of unknown function involved in DNA repair
0.237 5.72E-44 YLR417W VPS36 hom Component of the ESCRT-II complex; contains the GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into the endosome
0.233 1.06E-42 YDR372C VPS74 hom Golgi phosphatidylinositol-4-kinase effector and PtdIns4P sensor; interacts with the cytosolic domains of cis and medial glycosyltransferases, and in the PtdIns4P-bound state mediates the targeting of these enzymes to the Golgi; interacts with the catalytic domain of Sac1p, the major cellular PtdIns4P phosphatase, to direct dephosphosphorylation of the Golgi pool of PtdIns4P; tetramerization required for function; ortholog of human GOLPH3/GPP34/GMx33
0.227 2.51E-40 YPL065W VPS28 hom Component of the ESCRT-I complex (Stp22p, Srn2p, Vps28p, and Mvb12p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; conserved C-terminal domain interacts with ESCRT-III subunit Vps20p
0.227 2.56E-40 YDR455C_d YDR455C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YDR456W
0.219 9.82E-38 YAL002W VPS8 hom Membrane-binding component of the CORVET complex; involved in endosomal vesicle tethering and fusion in the endosome to vacuole protein targeting pathway; interacts with Vps21p; contains RING finger motif
0.213 8.15E-36 YOR275C RIM20 hom Protein involved in proteolytic activation of Rim101p in response to alkaline pH; PalA/AIP1/Alix family member; interaction with the ESCRT-III subunit Snf7p suggests a relationship between pH response and multivesicular body formation
0.213 1.17E-35 YOR089C VPS21 hom Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication
0.205 2.74E-33 YGR202C PCT1 hom Cholinephosphate cytidylyltransferase; a rate-determining enzyme of the CDP-choline pathway for phosphatidylcholine synthesis, inhibited by Sec14p, activated upon lipid-binding; contains an element within the regulatory domain involved in both silencing and activation of enzymatic activity