YLR394W / CST9

SUMO E3 ligase; required for synaptonemal complex formation; localizes to synapsis initiation sites on meiotic chromosomes; potential Cdc28p substrate

Zygosity: Homozygous strain
fixedexpanded
Profile for YLR394W / CST9

Click on Significant Values for Screen Details ID:SGTC_260|Compound:nsc-65238|FD-Score:3.16|P-value:5.42E-4 ID:SGTC_264|Compound:promethazine|FD-Score:-3.44|P-value:1.50E-4 ID:SGTC_315|Compound:k915-0449|FD-Score:3.47|P-value:1.64E-4 ID:SGTC_651|Compound:3253-0966|FD-Score:3.20|P-value:4.74E-4 ID:SGTC_658|Compound:3253-0951|FD-Score:-3.46|P-value:1.39E-4 ID:SGTC_835|Compound:0312-0010|FD-Score:-3.09|P-value:5.83E-4 ID:SGTC_914|Compound:3820-6779|FD-Score:-4.85|P-value:1.88E-7 ID:SGTC_918|Compound:glabranin|FD-Score:-3.74|P-value:4.31E-5 ID:SGTC_948|Compound:3448-0295|FD-Score:-3.00|P-value:8.14E-4 ID:SGTC_996|Compound:3970-0799|FD-Score:3.99|P-value:1.82E-5 ID:SGTC_1030|Compound:k029-0042|FD-Score:3.57|P-value:1.12E-4 ID:SGTC_1121|Compound:fusaric acid|FD-Score:3.21|P-value:4.50E-4 ID:SGTC_1125|Compound:tnp00248|FD-Score:3.95|P-value:2.13E-5 ID:SGTC_1326|Compound:1431-2094|FD-Score:3.03|P-value:8.54E-4 ID:SGTC_1462|Compound:k081-0014|FD-Score:-4.35|P-value:2.57E-6 ID:SGTC_1563|Compound:conessine|FD-Score:3.91|P-value:2.52E-5 ID:SGTC_1566|Compound:6,7-dimethoxy-4-ethylcoumarin|FD-Score:3.75|P-value:5.08E-5 ID:SGTC_1569|Compound:carminic acid|FD-Score:4.37|P-value:2.94E-6 ID:SGTC_1572|Compound:epiandrosterone|FD-Score:3.51|P-value:1.41E-4 ID:SGTC_1610|Compound:st001799|FD-Score:-3.02|P-value:7.37E-4 ID:SGTC_1763|Compound:st044482|FD-Score:3.28|P-value:3.48E-4 ID:SGTC_1774|Compound:st048371|FD-Score:3.21|P-value:4.47E-4 ID:SGTC_1777|Compound:st045864|FD-Score:2.99|P-value:9.92E-4 ID:SGTC_1906|Compound:st060206|FD-Score:4.22|P-value:6.06E-6 ID:SGTC_1944|Compound:st076371|FD-Score:-3.18|P-value:4.23E-4 ID:SGTC_1945|Compound:st075991|FD-Score:-3.56|P-value:9.19E-5 ID:SGTC_1946|Compound:st076057|FD-Score:4.14|P-value:9.02E-6 ID:SGTC_1994|Compound:st072947|FD-Score:-2.99|P-value:8.36E-4 ID:SGTC_2056|Compound:5235370|FD-Score:-5.21|P-value:2.33E-8 ID:SGTC_2097|Compound:5376186|FD-Score:-3.41|P-value:1.67E-4 ID:SGTC_2112|Compound:5488546|FD-Score:3.55|P-value:1.21E-4 ID:SGTC_2214|Compound:7179297|FD-Score:4.00|P-value:1.68E-5 ID:SGTC_2228|Compound:7354201|FD-Score:3.37|P-value:2.44E-4 ID:SGTC_2564|Compound:betulinic acid|FD-Score:3.21|P-value:4.50E-4 ID:SGTC_2601|Compound:securinine|FD-Score:-5.14|P-value:3.50E-8 ID:SGTC_2603|Compound:homopterocarpin|FD-Score:-3.83|P-value:2.93E-5 ID:SGTC_2749|Compound:bexarotene|FD-Score:-3.60|P-value:7.74E-5 ID:SGTC_2907|Compound:7721985|FD-Score:3.10|P-value:6.84E-4 ID:SGTC_2909|Compound:7941780|FD-Score:3.67|P-value:7.20E-5 ID:SGTC_3050|Compound:9093513|FD-Score:-3.92|P-value:1.94E-5 ID:SGTC_3089|Compound:9117425|FD-Score:3.02|P-value:8.84E-4 ID:SGTC_3270|Compound:9137563|FD-Score:-4.56|P-value:8.65E-7 ID:SGTC_3272|Compound:9137413|FD-Score:-3.85|P-value:2.70E-5 ID:SGTC_3294|Compound:9122953|FD-Score:3.01|P-value:9.45E-4 ID:SGTC_260|Compound:nsc-65238|FD-Score:3.16|P-value:5.42E-4 ID:SGTC_264|Compound:promethazine|FD-Score:-3.44|P-value:1.50E-4 ID:SGTC_315|Compound:k915-0449|FD-Score:3.47|P-value:1.64E-4 ID:SGTC_651|Compound:3253-0966|FD-Score:3.20|P-value:4.74E-4 ID:SGTC_658|Compound:3253-0951|FD-Score:-3.46|P-value:1.39E-4 ID:SGTC_835|Compound:0312-0010|FD-Score:-3.09|P-value:5.83E-4 ID:SGTC_914|Compound:3820-6779|FD-Score:-4.85|P-value:1.88E-7 ID:SGTC_918|Compound:glabranin|FD-Score:-3.74|P-value:4.31E-5 ID:SGTC_948|Compound:3448-0295|FD-Score:-3.00|P-value:8.14E-4 ID:SGTC_996|Compound:3970-0799|FD-Score:3.99|P-value:1.82E-5 ID:SGTC_1030|Compound:k029-0042|FD-Score:3.57|P-value:1.12E-4 ID:SGTC_1121|Compound:fusaric acid|FD-Score:3.21|P-value:4.50E-4 ID:SGTC_1125|Compound:tnp00248|FD-Score:3.95|P-value:2.13E-5 ID:SGTC_1326|Compound:1431-2094|FD-Score:3.03|P-value:8.54E-4 ID:SGTC_1462|Compound:k081-0014|FD-Score:-4.35|P-value:2.57E-6 ID:SGTC_1563|Compound:conessine|FD-Score:3.91|P-value:2.52E-5 ID:SGTC_1566|Compound:6,7-dimethoxy-4-ethylcoumarin|FD-Score:3.75|P-value:5.08E-5 ID:SGTC_1569|Compound:carminic acid|FD-Score:4.37|P-value:2.94E-6 ID:SGTC_1572|Compound:epiandrosterone|FD-Score:3.51|P-value:1.41E-4 ID:SGTC_1610|Compound:st001799|FD-Score:-3.02|P-value:7.37E-4 ID:SGTC_1763|Compound:st044482|FD-Score:3.28|P-value:3.48E-4 ID:SGTC_1774|Compound:st048371|FD-Score:3.21|P-value:4.47E-4 ID:SGTC_1777|Compound:st045864|FD-Score:2.99|P-value:9.92E-4 ID:SGTC_1906|Compound:st060206|FD-Score:4.22|P-value:6.06E-6 ID:SGTC_1944|Compound:st076371|FD-Score:-3.18|P-value:4.23E-4 ID:SGTC_1945|Compound:st075991|FD-Score:-3.56|P-value:9.19E-5 ID:SGTC_1946|Compound:st076057|FD-Score:4.14|P-value:9.02E-6 ID:SGTC_1994|Compound:st072947|FD-Score:-2.99|P-value:8.36E-4 ID:SGTC_2056|Compound:5235370|FD-Score:-5.21|P-value:2.33E-8 ID:SGTC_2097|Compound:5376186|FD-Score:-3.41|P-value:1.67E-4 ID:SGTC_2112|Compound:5488546|FD-Score:3.55|P-value:1.21E-4 ID:SGTC_2214|Compound:7179297|FD-Score:4.00|P-value:1.68E-5 ID:SGTC_2228|Compound:7354201|FD-Score:3.37|P-value:2.44E-4 ID:SGTC_2564|Compound:betulinic acid|FD-Score:3.21|P-value:4.50E-4 ID:SGTC_2601|Compound:securinine|FD-Score:-5.14|P-value:3.50E-8 ID:SGTC_2603|Compound:homopterocarpin|FD-Score:-3.83|P-value:2.93E-5 ID:SGTC_2749|Compound:bexarotene|FD-Score:-3.60|P-value:7.74E-5 ID:SGTC_2907|Compound:7721985|FD-Score:3.10|P-value:6.84E-4 ID:SGTC_2909|Compound:7941780|FD-Score:3.67|P-value:7.20E-5 ID:SGTC_3050|Compound:9093513|FD-Score:-3.92|P-value:1.94E-5 ID:SGTC_3089|Compound:9117425|FD-Score:3.02|P-value:8.84E-4 ID:SGTC_3270|Compound:9137563|FD-Score:-4.56|P-value:8.65E-7 ID:SGTC_3272|Compound:9137413|FD-Score:-3.85|P-value:2.70E-5 ID:SGTC_3294|Compound:9122953|FD-Score:3.01|P-value:9.45E-4

Top fitness defect scores for YLR394W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1569 carminic acid 4.37 40.6 µM 2.94E-6 11 6.17E-6
2 SGTC_1906 st060206 4.22 94.7 µM 6.06E-6 14 1.21E-5
3 SGTC_1946 st076057 4.14 65.1 µM 9.02E-6 2 1.75E-5
4 SGTC_2214 7179297 4.00 48.3 µM 1.68E-5 superoxide 6 3.12E-5
5 SGTC_996 3970-0799 3.99 1.8 µM 1.82E-5 83 3.36E-5
6 SGTC_1125 tnp00248 3.95 1.3 µM 2.13E-5 24 3.88E-5
7 SGTC_1563 conessine 3.91 56.1 µM 2.52E-5 18 4.55E-5
8 SGTC_1566 6,7-dimethoxy-4-ethylcoumarin 3.75 85.4 µM 5.08E-5 27 8.71E-5
9 SGTC_2909 7941780 3.67 10.5 µM 7.20E-5 25 1.20E-4
10 SGTC_1030 k029-0042 3.57 36.7 µM 1.12E-4 TSC3-RPN4 40 1.81E-4
11 SGTC_2112 5488546 3.55 200.0 µM 1.21E-4 TSC3-RPN4 26 1.95E-4
12 SGTC_1572 epiandrosterone 3.51 68.9 µM 1.41E-4 38 2.25E-4
13 SGTC_315 k915-0449 3.47 187.0 µM 1.64E-4 72 2.60E-4
14 SGTC_2228 7354201 3.37 200.0 µM 2.44E-4 endomembrane recycling 5 3.74E-4
15 SGTC_1763 st044482 3.28 94.5 µM 3.48E-4 copper-dependent oxidative stress 36 5.22E-4
16 SGTC_1774 st048371 3.21 48.5 µM 4.47E-4 RNA processing & uracil transport 50 6.59E-4
17 SGTC_1121 fusaric acid 3.21 630.5 nM 4.50E-4 67 6.63E-4
18 SGTC_2564 betulinic acid 3.21 100.0 µM 4.50E-4 63 6.63E-4
19 SGTC_651 3253-0966 3.20 34.4 µM 4.74E-4 calcium & mitochondrial duress 68 6.96E-4
20 SGTC_260 nsc-65238 3.16 4.0 µM 5.42E-4 endomembrane recycling 39 7.89E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.233 1.50E-42 YML118W NGL3 hom 3'-5' exonuclease specific for poly-A RNAs; has a domain similar to a magnesium-dependent endonuclease motif in mRNA deadenylase Ccr4p; similar to Ngl1p; NGL3 has a paralog, NGL2, that arose from the whole genome duplication
0.216 7.24E-37 YDR306C_p YDR306C_p hom F-box protein of unknown function; interacts with Sgt1p via a Leucine-Rich Repeat (LRR) domain
0.210 9.25E-35 YDL099W BUG1 hom Cis-golgi localized protein involved in ER to Golgi transport; forms a complex with the mammalian GRASP65 homolog, Grh1p; mutants are compromised for the fusion of ER-derived vesicles with Golgi membranes
0.162 4.61E-21 YDR505C PSP1 hom Asn and gln rich protein of unknown function; high-copy suppressor of POL1 (DNA polymerase alpha) and partial suppressor of CDC2 (polymerase delta) and CDC6 (pre-RC loading factor) mutations; overexpression results in growth inhibition; PSP1 has a paralog, YLR177W, that arose from the whole genome duplication
0.144 6.81E-17 YDL186W_p YDL186W_p hom Putative protein of unknown function; YDL186W is not an essential gene
0.138 1.24E-15 YGR048W UFD1 het Substrate-recruiting cofactor of the Cdc48p-Npl4p-Ufd1p segregase; polyubiquitin binding protein that assists in the dislocation of misfolded, ERAD substrates that are subsequently delivered to the proteasome for degradation; involved in regulated destruction of ER membrane proteins such as HMG-CoA reductase (Hmg1/2p) and cytoplasmic proteins (Fbp1p); involved in mobilizing membrane bound transaction factors by regulated Ub/proteasome-dependent processing (RUP)
0.135 4.99E-15 YMR107W SPG4 hom Protein required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources
0.132 1.58E-14 YDR315C IPK1 hom Inositol 1,3,4,5,6-pentakisphosphate 2-kinase, nuclear protein required for synthesis of 1,2,3,4,5,6-hexakisphosphate (phytate), which is integral to cell function; has 2 motifs conserved in other fungi; ipk1 gle1 double mutant is inviable
0.132 1.63E-14 YDR096W GIS1 hom Histone demethylase and transcription factor; regulates genes during nutrient limitation; negatively regulates DPP1, PHR1; activity modulated by limited proteasome-mediated proteolysis; has JmjC and JmjN domain in N-terminal region that interact, promoting stability and proper transcriptional activity; contains two transactivating domains downstream of Jmj domains and a C-terminal DNA binding domain; GIS1 has a paralog, RPH1, that arose from the whole genome duplication
0.129 7.00E-14 YGR201C_p YGR201C_p hom Putative protein of unknown function
0.128 1.06E-13 YGL040C HEM2 het Aminolevulinate dehydratase, a homo-octameric enzyme, catalyzes the conversion of 5-aminolevulinate to porphobilinogen, the second step in heme biosynthesis; enzymatic activity is zinc-dependent; localizes to the cytoplasm and nucleus
0.126 2.85E-13 YGL062W PYC1 hom Pyruvate carboxylase isoform; cytoplasmic enzyme that converts pyruvate to oxaloacetate; differentially regulated than isoform Pyc2p; mutations in the human homolog are associated with lactic acidosis; PYC1 has a paralog, PYC2, that arose from the whole genome duplication
0.125 3.42E-13 YFL047W RGD2 hom GTPase-activating protein (RhoGAP) for Cdc42p and Rho5p; relocalizes from bud neck to cytoplasm upon DNA replication stress
0.124 6.21E-13 YKL130C SHE2 hom RNA-binding protein that binds specific mRNAs and interacts with She3p; part of the mRNA localization machinery that restricts accumulation of certain proteins to the bud
0.122 1.25E-12 YNL149C PGA2 het Essential protein required for maturation of Gas1p and Pho8p; involved in protein trafficking; GFP-fusion protein localizes to the ER and YFP-fusion protein to the nuclear envelope-ER network; null mutants have a cell separation defect