YLR401C / DUS3

Dihydrouridine synthase; member of a widespread family of conserved proteins including Smm1p, Dus1p, and Dus4p; contains a consensus oleate response element (ORE) in its promoter region; forms nuclear foci upon DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YLR401C / DUS3

Click on Significant Values for Screen Details ID:SGTC_111|Compound:0083-0039|FD-Score:3.47|P-value:1.47E-5 ID:SGTC_112|Compound:phenothiazine|FD-Score:3.00|P-value:1.46E-4 ID:SGTC_750|Compound:4073-0104|FD-Score:-5.36|P-value:1.63E-10 ID:SGTC_807|Compound:1315-0151|FD-Score:2.89|P-value:2.35E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-4.14|P-value:6.54E-7 ID:SGTC_1270|Compound:0831-1170|FD-Score:2.87|P-value:2.59E-4 ID:SGTC_1459|Compound:k035-0031|FD-Score:-3.18|P-value:1.08E-4 ID:SGTC_1504|Compound:k297-0033|FD-Score:-3.13|P-value:1.37E-4 ID:SGTC_1508|Compound:1080-0625|FD-Score:2.90|P-value:2.27E-4 ID:SGTC_1689|Compound:st020265|FD-Score:2.58|P-value:8.98E-4 ID:SGTC_1708|Compound:st033234|FD-Score:-2.96|P-value:2.95E-4 ID:SGTC_1713|Compound:st033146|FD-Score:5.14|P-value:3.62E-10 ID:SGTC_1715|Compound:st032160|FD-Score:5.26|P-value:1.43E-10 ID:SGTC_1717|Compound:st033143|FD-Score:13.10|P-value:8.68E-55 ID:SGTC_1721|Compound:st082152|FD-Score:-8.30|P-value:7.13E-23 ID:SGTC_1732|Compound:st034308|FD-Score:-2.92|P-value:3.42E-4 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:-3.11|P-value:1.45E-4 ID:SGTC_2112|Compound:5488546|FD-Score:-2.96|P-value:2.86E-4 ID:SGTC_2313|Compound:7726885|FD-Score:3.54|P-value:9.78E-6 ID:SGTC_2352|Compound:9027995|FD-Score:-3.38|P-value:4.11E-5 ID:SGTC_2614|Compound:arthonioic acid|FD-Score:-2.86|P-value:4.53E-4 ID:SGTC_3206|Compound:9114350|FD-Score:-2.71|P-value:8.30E-4 ID:SGTC_111|Compound:0083-0039|FD-Score:3.47|P-value:1.47E-5 ID:SGTC_112|Compound:phenothiazine|FD-Score:3.00|P-value:1.46E-4 ID:SGTC_750|Compound:4073-0104|FD-Score:-5.36|P-value:1.63E-10 ID:SGTC_807|Compound:1315-0151|FD-Score:2.89|P-value:2.35E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-4.14|P-value:6.54E-7 ID:SGTC_1270|Compound:0831-1170|FD-Score:2.87|P-value:2.59E-4 ID:SGTC_1459|Compound:k035-0031|FD-Score:-3.18|P-value:1.08E-4 ID:SGTC_1504|Compound:k297-0033|FD-Score:-3.13|P-value:1.37E-4 ID:SGTC_1508|Compound:1080-0625|FD-Score:2.90|P-value:2.27E-4 ID:SGTC_1689|Compound:st020265|FD-Score:2.58|P-value:8.98E-4 ID:SGTC_1708|Compound:st033234|FD-Score:-2.96|P-value:2.95E-4 ID:SGTC_1713|Compound:st033146|FD-Score:5.14|P-value:3.62E-10 ID:SGTC_1715|Compound:st032160|FD-Score:5.26|P-value:1.43E-10 ID:SGTC_1717|Compound:st033143|FD-Score:13.10|P-value:8.68E-55 ID:SGTC_1721|Compound:st082152|FD-Score:-8.30|P-value:7.13E-23 ID:SGTC_1732|Compound:st034308|FD-Score:-2.92|P-value:3.42E-4 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:-3.11|P-value:1.45E-4 ID:SGTC_2112|Compound:5488546|FD-Score:-2.96|P-value:2.86E-4 ID:SGTC_2313|Compound:7726885|FD-Score:3.54|P-value:9.78E-6 ID:SGTC_2352|Compound:9027995|FD-Score:-3.38|P-value:4.11E-5 ID:SGTC_2614|Compound:arthonioic acid|FD-Score:-2.86|P-value:4.53E-4 ID:SGTC_3206|Compound:9114350|FD-Score:-2.71|P-value:8.30E-4

Top fitness defect scores for YLR401C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1717 st033143 13.10 58.0 µM 8.68E-55 1 2.98E-39
2 SGTC_1715 st032160 5.26 10.2 µM 1.43E-10 22 7.13E-8
3 SGTC_1713 st033146 5.14 12.8 µM 3.62E-10 8 1.38E-7
4 SGTC_2313 7726885 3.54 200.0 µM 9.78E-6 RPP1 & pyrimidine depletion 47 1.97E-4
5 SGTC_111 0083-0039 3.47 52.5 µM 1.47E-5 8 2.63E-4
6 SGTC_112 phenothiazine 3.00 287.2 µM 1.46E-4 unfolded protein response 43 0.00136
7 SGTC_1508 1080-0625 2.90 72.9 µM 2.27E-4 52 0.00186
8 SGTC_807 1315-0151 2.89 48.0 µM 2.35E-4 79 0.00191
9 SGTC_1270 0831-1170 2.87 17.3 µM 2.59E-4 58 0.00205
10 SGTC_1689 st020265 2.58 53.5 µM 8.98E-4 84 0.00499
11 SGTC_1282 0886-0010 2.50 32.4 µM 0.00120 endomembrane recycling 136 0.00616
12 SGTC_1988 st071872 2.49 53.4 µM 0.00126 93 0.00637
13 SGTC_2509 methyl 7-deshydroxypyrogallin-4-carboxylate 2.48 54.6 µM 0.00134 RPP1 & pyrimidine depletion 138 0.00665
14 SGTC_1686 st019653 2.44 115.5 µM 0.00156 mitochondrial processes 95 0.00741
15 SGTC_2045 5106652 2.38 78.0 µM 0.00196 101 0.00876
16 SGTC_2928 9011484 2.35 9.8 µM 0.00214 heme biosynthesis & mitochondrial translocase 128 0.00933
17 SGTC_2444 5485484 2.33 36.4 µM 0.00229 173 0.00979
18 SGTC_551 k048-0028 2.32 34.9 µM 0.00243 155 0.01020
19 SGTC_2307 7538926 2.30 6.3 µM 0.00263 190 0.01080
20 SGTC_110 0850-0284 2.29 111.6 µM 0.00265 76 0.01090

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.338 3.48E-90 YER011W TIR1 hom Cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; expression is downregulated at acidic pH and induced by cold shock and anaerobiosis; abundance is increased in cells cultured without shaking
0.301 2.11E-71 YMR195W ICY1 hom Protein of unknown function, required for viability in rich media of cells lacking mitochondrial DNA; mutants have an invasive growth defect with elongated morphology; induced by amino acid starvation
0.236 8.53E-44 YOR012W_p YOR012W_p hom Putative protein of unknown function
0.230 1.18E-41 YGR032W GSC2 hom Catalytic subunit of 1,3-beta-glucan synthase, involved in formation of the inner layer of the spore wall; activity positively regulated by Rho1p and negatively by Smk1p; has similarity to an alternate catalytic subunit, Fks1p (Gsc1p)
0.226 2.93E-40 YJL060W BNA3 hom Kynurenine aminotransferase, catalyzes formation of kynurenic acid from kynurenine; potential Cdc28p substrate
0.209 2.33E-34 YKR014C YPT52 hom Rab family GTPase, similar to Ypt51p and Ypt53p and to mammalian rab5; required for vacuolar protein sorting and endocytosis; protein abundance increases in response to DNA replication stress
0.204 7.80E-33 YJL083W TAX4 hom EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; TAX4 has a paralog, IRS4, that arose from the whole genome duplication
0.202 2.37E-32 YBL036C YBL036C hom Putative non-specific single-domain racemase based on structural similarity; binds pyridoxal 5'-phosphate; expression of GFP-fusion protein induced in response to the DNA-damaging agent MMS
0.198 5.52E-31 YER064C VHR2 hom Non-essential nuclear protein; null mutation has global effects on transcription; VHR2 has a paralog, VHR1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress
0.192 2.65E-29 YAL068C PAU8 hom Protein of unknown function, member of the seripauperin multigene family encoded mainly in subtelomeric regions
0.189 2.75E-28 YIL010W DOT5 hom Nuclear thiol peroxidase which functions as an alkyl-hydroperoxide reductase during post-diauxic growth
0.185 2.36E-27 YNL099C OCA1 hom Putative protein tyrosine phosphatase, required for cell cycle arrest in response to oxidative damage of DNA
0.185 3.97E-27 YJL068C YJL068C hom Non-essential intracellular esterase that can function as an S-formylglutathione hydrolase; may be involved in the detoxification of formaldehyde, which can be metabolized to S-formylglutathione; similar to human esterase D
0.181 5.21E-26 YOR298C-A MBF1 hom Transcriptional coactivator; bridges the DNA-binding region of Gcn4p and TATA-binding protein Spt15p; suppressor of frameshift mutations; protein abundance increases in response to DNA replication stress
0.178 3.10E-25 YHR003C YHR003C hom Protein of unknown function, localized to the mitochondrial outer membrane