YLR414C / PUN1

Plasma membrane protein with a role in cell wall integrity; co-localizes with Sur7p in punctate membrane patches; null mutant displays decreased thermotolerance; transcription induced upon cell wall damage and metal ion stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YLR414C / PUN1

Click on Significant Values for Screen Details ID:SGTC_6|Compound:0844-0013|FD-Score:3.59|P-value:3.10E-5 ID:SGTC_32|Compound:0928-0142|FD-Score:4.03|P-value:3.40E-6 ID:SGTC_37|Compound:0566-0070|FD-Score:3.51|P-value:4.31E-5 ID:SGTC_42|Compound:0133-0086|FD-Score:-4.42|P-value:7.73E-7 ID:SGTC_66|Compound:0109-0187|FD-Score:-15.40|P-value:1.36E-64 ID:SGTC_68|Compound:0986-0284|FD-Score:3.90|P-value:6.69E-6 ID:SGTC_385|Compound:1494-0393|FD-Score:-3.36|P-value:1.37E-4 ID:SGTC_447|Compound:ml7|FD-Score:3.08|P-value:2.82E-4 ID:SGTC_448|Compound:monastrol|FD-Score:4.01|P-value:3.83E-6 ID:SGTC_460|Compound:trequinsin|FD-Score:3.04|P-value:3.30E-4 ID:SGTC_510|Compound:ag-490|FD-Score:2.85|P-value:6.87E-4 ID:SGTC_600|Compound:1495-0418|FD-Score:-2.95|P-value:7.31E-4 ID:SGTC_614|Compound:0462-0418|FD-Score:-3.46|P-value:8.96E-5 ID:SGTC_843|Compound:0310-0051|FD-Score:3.18|P-value:1.84E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-5.40|P-value:1.96E-9 ID:SGTC_1114|Compound:scopoletin|FD-Score:3.64|P-value:2.38E-5 ID:SGTC_1335|Compound:1486-0900|FD-Score:-3.07|P-value:4.48E-4 ID:SGTC_1385|Compound:3091-4834|FD-Score:3.00|P-value:3.81E-4 ID:SGTC_1391|Compound:3442-0225|FD-Score:2.94|P-value:4.97E-4 ID:SGTC_1459|Compound:k035-0031|FD-Score:-4.00|P-value:7.24E-6 ID:SGTC_1481|Compound:3346-2004|FD-Score:-3.77|P-value:2.16E-5 ID:SGTC_1960|Compound:st011366|FD-Score:-3.05|P-value:4.87E-4 ID:SGTC_2050|Compound:5263056|FD-Score:4.57|P-value:1.75E-7 ID:SGTC_2052|Compound:5265182|FD-Score:3.92|P-value:6.09E-6 ID:SGTC_2087|Compound:5276107|FD-Score:-3.96|P-value:8.75E-6 ID:SGTC_2345|Compound:9052315|FD-Score:-3.68|P-value:3.26E-5 ID:SGTC_2416|Compound:tofa|FD-Score:4.01|P-value:3.85E-6 ID:SGTC_2419|Compound:paclitaxel|FD-Score:2.93|P-value:5.10E-4 ID:SGTC_2468|Compound:5987765|FD-Score:4.09|P-value:2.53E-6 ID:SGTC_2475|Compound:5812728|FD-Score:-3.95|P-value:8.92E-6 ID:SGTC_2569|Compound:tetrandrine|FD-Score:-3.61|P-value:4.49E-5 ID:SGTC_2572|Compound:astragaloside iv|FD-Score:3.08|P-value:2.83E-4 ID:SGTC_2579|Compound:2',5'-dihydroxy-4-methoxychalcone|FD-Score:-3.26|P-value:2.08E-4 ID:SGTC_2821|Compound:7996764|FD-Score:3.82|P-value:9.86E-6 ID:SGTC_2835|Compound:9006588|FD-Score:-3.77|P-value:2.21E-5 ID:SGTC_2922|Compound:7999257|FD-Score:-2.92|P-value:7.93E-4 ID:SGTC_2973|Compound:9094665|FD-Score:-3.56|P-value:5.71E-5 ID:SGTC_2992|Compound:9074591|FD-Score:-3.49|P-value:7.71E-5 ID:SGTC_2998|Compound:9066762|FD-Score:2.93|P-value:5.12E-4 ID:SGTC_3002|Compound:9075603|FD-Score:-3.20|P-value:2.66E-4 ID:SGTC_3005|Compound:9076496|FD-Score:-3.39|P-value:1.20E-4 ID:SGTC_3045|Compound:9092950|FD-Score:2.83|P-value:7.41E-4 ID:SGTC_3048|Compound:9093155|FD-Score:3.53|P-value:4.03E-5 ID:SGTC_3097|Compound:9117246|FD-Score:-4.04|P-value:5.83E-6 ID:SGTC_3159|Compound:9100096|FD-Score:-2.93|P-value:7.66E-4 ID:SGTC_3175|Compound:9106517|FD-Score:3.55|P-value:3.73E-5 ID:SGTC_3317|Compound:9138495|FD-Score:-3.02|P-value:5.44E-4 ID:SGTC_6|Compound:0844-0013|FD-Score:3.59|P-value:3.10E-5 ID:SGTC_32|Compound:0928-0142|FD-Score:4.03|P-value:3.40E-6 ID:SGTC_37|Compound:0566-0070|FD-Score:3.51|P-value:4.31E-5 ID:SGTC_42|Compound:0133-0086|FD-Score:-4.42|P-value:7.73E-7 ID:SGTC_66|Compound:0109-0187|FD-Score:-15.40|P-value:1.36E-64 ID:SGTC_68|Compound:0986-0284|FD-Score:3.90|P-value:6.69E-6 ID:SGTC_385|Compound:1494-0393|FD-Score:-3.36|P-value:1.37E-4 ID:SGTC_447|Compound:ml7|FD-Score:3.08|P-value:2.82E-4 ID:SGTC_448|Compound:monastrol|FD-Score:4.01|P-value:3.83E-6 ID:SGTC_460|Compound:trequinsin|FD-Score:3.04|P-value:3.30E-4 ID:SGTC_510|Compound:ag-490|FD-Score:2.85|P-value:6.87E-4 ID:SGTC_600|Compound:1495-0418|FD-Score:-2.95|P-value:7.31E-4 ID:SGTC_614|Compound:0462-0418|FD-Score:-3.46|P-value:8.96E-5 ID:SGTC_843|Compound:0310-0051|FD-Score:3.18|P-value:1.84E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-5.40|P-value:1.96E-9 ID:SGTC_1114|Compound:scopoletin|FD-Score:3.64|P-value:2.38E-5 ID:SGTC_1335|Compound:1486-0900|FD-Score:-3.07|P-value:4.48E-4 ID:SGTC_1385|Compound:3091-4834|FD-Score:3.00|P-value:3.81E-4 ID:SGTC_1391|Compound:3442-0225|FD-Score:2.94|P-value:4.97E-4 ID:SGTC_1459|Compound:k035-0031|FD-Score:-4.00|P-value:7.24E-6 ID:SGTC_1481|Compound:3346-2004|FD-Score:-3.77|P-value:2.16E-5 ID:SGTC_1960|Compound:st011366|FD-Score:-3.05|P-value:4.87E-4 ID:SGTC_2050|Compound:5263056|FD-Score:4.57|P-value:1.75E-7 ID:SGTC_2052|Compound:5265182|FD-Score:3.92|P-value:6.09E-6 ID:SGTC_2087|Compound:5276107|FD-Score:-3.96|P-value:8.75E-6 ID:SGTC_2345|Compound:9052315|FD-Score:-3.68|P-value:3.26E-5 ID:SGTC_2416|Compound:tofa|FD-Score:4.01|P-value:3.85E-6 ID:SGTC_2419|Compound:paclitaxel|FD-Score:2.93|P-value:5.10E-4 ID:SGTC_2468|Compound:5987765|FD-Score:4.09|P-value:2.53E-6 ID:SGTC_2475|Compound:5812728|FD-Score:-3.95|P-value:8.92E-6 ID:SGTC_2569|Compound:tetrandrine|FD-Score:-3.61|P-value:4.49E-5 ID:SGTC_2572|Compound:astragaloside iv|FD-Score:3.08|P-value:2.83E-4 ID:SGTC_2579|Compound:2',5'-dihydroxy-4-methoxychalcone|FD-Score:-3.26|P-value:2.08E-4 ID:SGTC_2821|Compound:7996764|FD-Score:3.82|P-value:9.86E-6 ID:SGTC_2835|Compound:9006588|FD-Score:-3.77|P-value:2.21E-5 ID:SGTC_2922|Compound:7999257|FD-Score:-2.92|P-value:7.93E-4 ID:SGTC_2973|Compound:9094665|FD-Score:-3.56|P-value:5.71E-5 ID:SGTC_2992|Compound:9074591|FD-Score:-3.49|P-value:7.71E-5 ID:SGTC_2998|Compound:9066762|FD-Score:2.93|P-value:5.12E-4 ID:SGTC_3002|Compound:9075603|FD-Score:-3.20|P-value:2.66E-4 ID:SGTC_3005|Compound:9076496|FD-Score:-3.39|P-value:1.20E-4 ID:SGTC_3045|Compound:9092950|FD-Score:2.83|P-value:7.41E-4 ID:SGTC_3048|Compound:9093155|FD-Score:3.53|P-value:4.03E-5 ID:SGTC_3097|Compound:9117246|FD-Score:-4.04|P-value:5.83E-6 ID:SGTC_3159|Compound:9100096|FD-Score:-2.93|P-value:7.66E-4 ID:SGTC_3175|Compound:9106517|FD-Score:3.55|P-value:3.73E-5 ID:SGTC_3317|Compound:9138495|FD-Score:-3.02|P-value:5.44E-4

Top fitness defect scores for YLR414C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2050 5263056 4.57 171.0 µM 1.75E-7 fatty acid desaturase (OLE1) 32 2.38E-6
2 SGTC_2468 5987765 4.09 72.9 µM 2.53E-6 11 2.14E-5
3 SGTC_32 0928-0142 4.03 34.1 µM 3.40E-6 TSC3-RPN4 7 2.73E-5
4 SGTC_448 monastrol 4.01 171.0 µM 3.83E-6 9 3.01E-5
5 SGTC_2416 tofa 4.01 880.0 nM 3.85E-6 sphingolipid biosynthesis & PDR1 49 3.02E-5
6 SGTC_2052 5265182 3.92 7.1 µM 6.09E-6 31 4.41E-5
7 SGTC_68 0986-0284 3.90 82.8 µM 6.69E-6 72 4.76E-5
8 SGTC_2821 7996764 3.82 71.4 µM 9.86E-6 46 6.56E-5
9 SGTC_1114 scopoletin 3.64 1.7 µM 2.38E-5 25 1.36E-4
10 SGTC_6 0844-0013 3.59 19.8 µM 3.10E-5 129 1.68E-4
11 SGTC_3175 9106517 3.55 49.5 µM 3.73E-5 iron homeostasis 24 1.96E-4
12 SGTC_3048 9093155 3.53 49.5 µM 4.03E-5 11 2.09E-4
13 SGTC_37 0566-0070 3.51 1.6 µM 4.31E-5 41 2.21E-4
14 SGTC_843 0310-0051 3.18 143.0 µM 1.84E-4 41 7.28E-4
15 SGTC_447 ml7 3.08 120.0 µM 2.82E-4 NEO1-PIK1 67 0.00104
16 SGTC_2572 astragaloside iv 3.08 100.0 µM 2.83E-4 69 0.00104
17 SGTC_460 trequinsin 3.04 123.0 µM 3.30E-4 13 0.00118
18 SGTC_1385 3091-4834 3.00 14.1 µM 3.81E-4 RPP1 & pyrimidine depletion 49 0.00133
19 SGTC_1391 3442-0225 2.94 151.0 µM 4.97E-4 52 0.00165
20 SGTC_2419 paclitaxel 2.93 166.5 µM 5.10E-4 117 0.00169

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.401 6.90E-130 YGL004C RPN14 hom Proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasome regulatory particle (RP); null mutants accumulate ubiquitinated Gcn4p and display decreased 26S proteasome stability; interacts with Rpt5p
0.257 8.87E-52 YLR263W RED1 hom Protein component of the synaptonemal complex axial elements, involved in chromosome segregation during the first meiotic division; critical for coupling checkpoint signaling to SC formation; interacts with Hop1p, Mec3p and Ddc1p
0.254 2.17E-50 YJL058C BIT61 hom Subunit of TORC2 membrane-associated complex; involved in regulation of cell cycle-dependent actin cytoskeletal dynamics during polarized growth and cell wall integrity; BIT61 has a paralog, BIT2, that arose from the whole genome duplication
0.247 1.15E-47 YGR139W_d YGR139W_d hom Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.241 1.23E-45 YKL131W_d YKL131W_d hom Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.233 1.92E-42 YLR205C HMX1 hom ER localized heme oxygenase, involved in heme degradation during iron starvation and in the oxidative stress response; expression is regulated by AFT1 and oxidative stress; relocates to the perinuclear region in the presence of oxidants
0.229 4.18E-41 YLR405W DUS4 hom Dihydrouridine synthase, member of a widespread family of conserved proteins including Smm1p, Dus1p, and Dus3p
0.222 1.20E-38 YJR106W ECM27 hom Putative protein of unknown function; may play a role in cell wall biosynthesis, mutants are hypersensitive to Papulacandin B; null mutants have increased plasmid loss; displays a two-hybrid interaction with Pdr5p
0.213 8.48E-36 YPR151C SUE1 hom Mitochondrial protein required for degradation of unstable forms of cytochrome c
0.210 1.26E-34 YJR108W ABM1 hom Protein of unknown function, required for normal microtubule organization
0.208 2.89E-34 YCL023C_d YCL023C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF KCC4
0.198 4.94E-31 YKL102C_d YKL102C_d hom Dubious open reading frame unlikely to encode a functional protein; deletion confers sensitivity to citric acid; predicted protein would include a thiol-disulfide oxidoreductase active site
0.193 1.30E-29 YKL024C URA6 het Uridylate kinase, catalyzes the seventh enzymatic step in the de novo biosynthesis of pyrimidines, converting uridine monophosphate (UMP) into uridine-5'-diphosphate (UDP)
0.187 7.27E-28 YMR041C ARA2 hom NAD-dependent arabinose dehydrogenase, involved in biosynthesis of dehydro-D-arabinono-1,4-lactone; similar to plant L-galactose dehydrogenase
0.182 2.13E-26 YGR008C STF2 hom Protein involved in resistance to dessication stress; Stf2p exhibits antioxidant properties, and its overexpression prevents ROS accumulation and apoptosis; binds to the F0 sector of mitochondrial F1F0 ATPase in vitro and is proposed to modulate the inhibitory action of Inh1p and Stf1p; protein abundance increases in response to DNA replication stress