YLR451W / LEU3

Zinc-knuckle transcription factor, repressor and activator; regulates genes involved in branched chain amino acid biosynthesis and ammonia assimilation; acts as a repressor in leucine-replete conditions and as an activator in the presence of alpha-isopropylmalate, an intermediate in leucine biosynthesis that accumulates during leucine starvation

Zygosity: Homozygous strain
fixedexpanded
Profile for YLR451W / LEU3

Click on Significant Values for Screen Details ID:SGTC_8|Compound:0354-0001|FD-Score:9.37|P-value:6.69E-22 ID:SGTC_59|Compound:0330-0160|FD-Score:8.47|P-value:2.93E-18 ID:SGTC_64|Compound:000l-0018|FD-Score:3.78|P-value:5.91E-5 ID:SGTC_73|Compound:0327-0325|FD-Score:-3.94|P-value:2.81E-5 ID:SGTC_87|Compound:0100-0052|FD-Score:6.49|P-value:1.89E-11 ID:SGTC_142|Compound:0335-0849|FD-Score:3.93|P-value:3.19E-5 ID:SGTC_158|Compound:k038-0087|FD-Score:4.87|P-value:3.42E-7 ID:SGTC_161|Compound:4065-0411|FD-Score:10.50|P-value:3.40E-27 ID:SGTC_164|Compound:4100-3994|FD-Score:-3.83|P-value:4.58E-5 ID:SGTC_167|Compound:3672-0051|FD-Score:-3.31|P-value:3.62E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:-4.57|P-value:1.51E-6 ID:SGTC_315|Compound:k915-0449|FD-Score:5.12|P-value:9.11E-8 ID:SGTC_322|Compound:k048-0136|FD-Score:3.47|P-value:2.09E-4 ID:SGTC_329|Compound:k048-0121|FD-Score:3.84|P-value:4.64E-5 ID:SGTC_356|Compound:1634-0012|FD-Score:3.10|P-value:8.08E-4 ID:SGTC_408|Compound:cantharidin|FD-Score:3.90|P-value:3.64E-5 ID:SGTC_476|Compound:mead acid|FD-Score:3.10|P-value:7.95E-4 ID:SGTC_558|Compound:k064-0027|FD-Score:-3.42|P-value:2.34E-4 ID:SGTC_568|Compound:1193-0097|FD-Score:3.49|P-value:1.93E-4 ID:SGTC_810|Compound:3825-7150|FD-Score:3.16|P-value:6.50E-4 ID:SGTC_812|Compound:4251-0442|FD-Score:3.77|P-value:6.15E-5 ID:SGTC_890|Compound:0929-0006|FD-Score:3.18|P-value:6.01E-4 ID:SGTC_904|Compound:1141-0088|FD-Score:-3.03|P-value:9.66E-4 ID:SGTC_939|Compound:1165-0479|FD-Score:-3.54|P-value:1.50E-4 ID:SGTC_942|Compound:3253-1115|FD-Score:-3.07|P-value:8.47E-4 ID:SGTC_1142|Compound:3253-1408|FD-Score:3.67|P-value:9.17E-5 ID:SGTC_1147|Compound:3809-1045|FD-Score:3.16|P-value:6.37E-4 ID:SGTC_1267|Compound:0828-0272|FD-Score:3.63|P-value:1.11E-4 ID:SGTC_1344|Compound:1488-0097|FD-Score:3.30|P-value:3.90E-4 ID:SGTC_1501|Compound:4491-0275|FD-Score:3.10|P-value:8.00E-4 ID:SGTC_1538|Compound:2',3',6-trimethoxyflavanone|FD-Score:3.24|P-value:4.91E-4 ID:SGTC_1634|Compound:st006576|FD-Score:3.26|P-value:4.54E-4 ID:SGTC_1647|Compound:st011722|FD-Score:3.52|P-value:1.68E-4 ID:SGTC_1690|Compound:st023502|FD-Score:3.48|P-value:1.99E-4 ID:SGTC_1820|Compound:st053765|FD-Score:3.11|P-value:7.84E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:-3.30|P-value:3.69E-4 ID:SGTC_1846|Compound:st056244|FD-Score:-3.47|P-value:2.00E-4 ID:SGTC_1850|Compound:st056238|FD-Score:4.03|P-value:2.06E-5 ID:SGTC_1860|Compound:st056228|FD-Score:-3.07|P-value:8.63E-4 ID:SGTC_1861|Compound:st057647|FD-Score:4.26|P-value:7.12E-6 ID:SGTC_1997|Compound:st073024|FD-Score:3.39|P-value:2.77E-4 ID:SGTC_2075|Compound:5212009|FD-Score:3.08|P-value:8.62E-4 ID:SGTC_2247|Compound:cerulenin|FD-Score:3.13|P-value:7.16E-4 ID:SGTC_2419|Compound:paclitaxel|FD-Score:4.08|P-value:1.62E-5 ID:SGTC_2429|Compound:5902844|FD-Score:3.53|P-value:1.64E-4 ID:SGTC_2528|Compound:xanthyletin|FD-Score:3.58|P-value:1.34E-4 ID:SGTC_2617|Compound:dihydrotanshinone i|FD-Score:-3.20|P-value:5.42E-4 ID:SGTC_2645|Compound:abamectin|FD-Score:3.36|P-value:3.12E-4 ID:SGTC_2651|Compound:1327-0036|FD-Score:3.98|P-value:2.50E-5 ID:SGTC_2688|Compound:kanamycin b|FD-Score:4.10|P-value:1.46E-5 ID:SGTC_2703|Compound:st077852|FD-Score:3.36|P-value:3.12E-4 ID:SGTC_2976|Compound:9009005|FD-Score:3.25|P-value:4.74E-4 ID:SGTC_3006|Compound:9075424|FD-Score:3.40|P-value:2.73E-4 ID:SGTC_3033|Compound:9090887|FD-Score:3.10|P-value:8.09E-4 ID:SGTC_3049|Compound:9092223|FD-Score:-3.45|P-value:2.11E-4 ID:SGTC_3131|Compound:9127911|FD-Score:3.42|P-value:2.47E-4 ID:SGTC_3166|Compound:9102297|FD-Score:-3.52|P-value:1.64E-4 ID:SGTC_3254|Compound:9136651|FD-Score:3.23|P-value:5.01E-4 ID:SGTC_3285|Compound:9112818|FD-Score:3.45|P-value:2.23E-4 ID:SGTC_3299|Compound:9121982|FD-Score:7.66|P-value:2.95E-15 ID:SGTC_8|Compound:0354-0001|FD-Score:9.37|P-value:6.69E-22 ID:SGTC_59|Compound:0330-0160|FD-Score:8.47|P-value:2.93E-18 ID:SGTC_64|Compound:000l-0018|FD-Score:3.78|P-value:5.91E-5 ID:SGTC_73|Compound:0327-0325|FD-Score:-3.94|P-value:2.81E-5 ID:SGTC_87|Compound:0100-0052|FD-Score:6.49|P-value:1.89E-11 ID:SGTC_142|Compound:0335-0849|FD-Score:3.93|P-value:3.19E-5 ID:SGTC_158|Compound:k038-0087|FD-Score:4.87|P-value:3.42E-7 ID:SGTC_161|Compound:4065-0411|FD-Score:10.50|P-value:3.40E-27 ID:SGTC_164|Compound:4100-3994|FD-Score:-3.83|P-value:4.58E-5 ID:SGTC_167|Compound:3672-0051|FD-Score:-3.31|P-value:3.62E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:-4.57|P-value:1.51E-6 ID:SGTC_315|Compound:k915-0449|FD-Score:5.12|P-value:9.11E-8 ID:SGTC_322|Compound:k048-0136|FD-Score:3.47|P-value:2.09E-4 ID:SGTC_329|Compound:k048-0121|FD-Score:3.84|P-value:4.64E-5 ID:SGTC_356|Compound:1634-0012|FD-Score:3.10|P-value:8.08E-4 ID:SGTC_408|Compound:cantharidin|FD-Score:3.90|P-value:3.64E-5 ID:SGTC_476|Compound:mead acid|FD-Score:3.10|P-value:7.95E-4 ID:SGTC_558|Compound:k064-0027|FD-Score:-3.42|P-value:2.34E-4 ID:SGTC_568|Compound:1193-0097|FD-Score:3.49|P-value:1.93E-4 ID:SGTC_810|Compound:3825-7150|FD-Score:3.16|P-value:6.50E-4 ID:SGTC_812|Compound:4251-0442|FD-Score:3.77|P-value:6.15E-5 ID:SGTC_890|Compound:0929-0006|FD-Score:3.18|P-value:6.01E-4 ID:SGTC_904|Compound:1141-0088|FD-Score:-3.03|P-value:9.66E-4 ID:SGTC_939|Compound:1165-0479|FD-Score:-3.54|P-value:1.50E-4 ID:SGTC_942|Compound:3253-1115|FD-Score:-3.07|P-value:8.47E-4 ID:SGTC_1142|Compound:3253-1408|FD-Score:3.67|P-value:9.17E-5 ID:SGTC_1147|Compound:3809-1045|FD-Score:3.16|P-value:6.37E-4 ID:SGTC_1267|Compound:0828-0272|FD-Score:3.63|P-value:1.11E-4 ID:SGTC_1344|Compound:1488-0097|FD-Score:3.30|P-value:3.90E-4 ID:SGTC_1501|Compound:4491-0275|FD-Score:3.10|P-value:8.00E-4 ID:SGTC_1538|Compound:2',3',6-trimethoxyflavanone|FD-Score:3.24|P-value:4.91E-4 ID:SGTC_1634|Compound:st006576|FD-Score:3.26|P-value:4.54E-4 ID:SGTC_1647|Compound:st011722|FD-Score:3.52|P-value:1.68E-4 ID:SGTC_1690|Compound:st023502|FD-Score:3.48|P-value:1.99E-4 ID:SGTC_1820|Compound:st053765|FD-Score:3.11|P-value:7.84E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:-3.30|P-value:3.69E-4 ID:SGTC_1846|Compound:st056244|FD-Score:-3.47|P-value:2.00E-4 ID:SGTC_1850|Compound:st056238|FD-Score:4.03|P-value:2.06E-5 ID:SGTC_1860|Compound:st056228|FD-Score:-3.07|P-value:8.63E-4 ID:SGTC_1861|Compound:st057647|FD-Score:4.26|P-value:7.12E-6 ID:SGTC_1997|Compound:st073024|FD-Score:3.39|P-value:2.77E-4 ID:SGTC_2075|Compound:5212009|FD-Score:3.08|P-value:8.62E-4 ID:SGTC_2247|Compound:cerulenin|FD-Score:3.13|P-value:7.16E-4 ID:SGTC_2419|Compound:paclitaxel|FD-Score:4.08|P-value:1.62E-5 ID:SGTC_2429|Compound:5902844|FD-Score:3.53|P-value:1.64E-4 ID:SGTC_2528|Compound:xanthyletin|FD-Score:3.58|P-value:1.34E-4 ID:SGTC_2617|Compound:dihydrotanshinone i|FD-Score:-3.20|P-value:5.42E-4 ID:SGTC_2645|Compound:abamectin|FD-Score:3.36|P-value:3.12E-4 ID:SGTC_2651|Compound:1327-0036|FD-Score:3.98|P-value:2.50E-5 ID:SGTC_2688|Compound:kanamycin b|FD-Score:4.10|P-value:1.46E-5 ID:SGTC_2703|Compound:st077852|FD-Score:3.36|P-value:3.12E-4 ID:SGTC_2976|Compound:9009005|FD-Score:3.25|P-value:4.74E-4 ID:SGTC_3006|Compound:9075424|FD-Score:3.40|P-value:2.73E-4 ID:SGTC_3033|Compound:9090887|FD-Score:3.10|P-value:8.09E-4 ID:SGTC_3049|Compound:9092223|FD-Score:-3.45|P-value:2.11E-4 ID:SGTC_3131|Compound:9127911|FD-Score:3.42|P-value:2.47E-4 ID:SGTC_3166|Compound:9102297|FD-Score:-3.52|P-value:1.64E-4 ID:SGTC_3254|Compound:9136651|FD-Score:3.23|P-value:5.01E-4 ID:SGTC_3285|Compound:9112818|FD-Score:3.45|P-value:2.23E-4 ID:SGTC_3299|Compound:9121982|FD-Score:7.66|P-value:2.95E-15

Top fitness defect scores for YLR451W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_161 4065-0411 10.50 162.2 µM 3.40E-27 5 3.05E-26
2 SGTC_8 0354-0001 9.37 70.0 µM 6.69E-22 5 3.79E-21
3 SGTC_59 0330-0160 8.47 1.3 µM 2.93E-18 13 1.21E-17
4 SGTC_3299 9121982 7.66 43.1 µM 2.95E-15 5 9.39E-15
5 SGTC_87 0100-0052 6.49 35.5 µM 1.89E-11 25 4.33E-11
6 SGTC_315 k915-0449 5.12 187.0 µM 9.11E-8 27 1.53E-7
7 SGTC_158 k038-0087 4.87 26.4 µM 3.42E-7 ubiquinone biosynthesis & proteasome 92 5.47E-7
8 SGTC_1861 st057647 4.26 17.4 µM 7.12E-6 14 1.02E-5
9 SGTC_2688 kanamycin b 4.10 96.7 µM 1.46E-5 calcium & mitochondrial duress 347 2.03E-5
10 SGTC_2419 paclitaxel 4.08 166.5 µM 1.62E-5 34 2.25E-5
11 SGTC_1850 st056238 4.03 17.0 µM 2.06E-5 RPP1 & pyrimidine depletion 18 2.84E-5
12 SGTC_2651 1327-0036 3.98 5.0 µM 2.50E-5 156 3.42E-5
13 SGTC_142 0335-0849 3.93 62.7 µM 3.19E-5 ubiquinone biosynthesis & proteasome 128 4.33E-5
14 SGTC_408 cantharidin 3.90 100.0 µM 3.64E-5 cell wall signaling 22 4.91E-5
15 SGTC_329 k048-0121 3.84 21.3 µM 4.64E-5 RSC & ERG11 14 6.21E-5
16 SGTC_64 000l-0018 3.78 272.1 µM 5.91E-5 ubiquinone biosynthesis & proteasome 161 7.84E-5
17 SGTC_812 4251-0442 3.77 148.0 µM 6.15E-5 11 8.14E-5
18 SGTC_1142 3253-1408 3.67 9.8 µM 9.17E-5 19 1.20E-4
19 SGTC_1267 0828-0272 3.63 9.5 µM 1.11E-4 30 1.44E-4
20 SGTC_2528 xanthyletin 3.58 44.8 µM 1.34E-4 31 1.73E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.233 1.69E-42 YNL056W OCA2 hom Putative protein with similarity to predicted tyrosine phosphatases Oca1p and Siw14p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YNL056W is not an essential gene
0.204 9.77E-33 YGR055W MUP1 hom High affinity methionine permease, integral membrane protein with 13 putative membrane-spanning regions; also involved in cysteine uptake
0.193 1.28E-29 YHL029C_p OCA5_p hom Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts
0.191 5.43E-29 YBR068C BAP2 hom High-affinity leucine permease; functions as a branched-chain amino acid permease involved in uptake of leucine, isoleucine and valine; contains 12 predicted transmembrane domains; BAP2 has a paralog, BAP3, that arose from the whole genome duplication
0.190 1.02E-28 YPL077C_p YPL077C_p hom Putative protein of unknown function; regulates PIS1 expression; mutant displays spore wall assembly defect in ether sensitivity screen; YPL077C is not an essential gene; YPL077C has a paralog, YBR197C, that arose from the whole genome duplication
0.186 1.29E-27 YEL007W MIT1 hom Transcriptional regulator of pseudohyphal growth; protein with sequence similarity to S. pombe gti1+ (gluconate transport inducer 1) and C. albicans Wor1
0.179 1.79E-25 YER086W ILV1 hom Threonine deaminase, catalyzes the first step in isoleucine biosynthesis; expression is under general amino acid control; ILV1 locus exhibits highly positioned nucleosomes whose organization is independent of known ILV1 regulation
0.176 8.16E-25 YPL160W CDC60 het Cytosolic leucyl tRNA synthetase, ligates leucine to the appropriate tRNA
0.173 5.27E-24 YCR095C_p OCA4_p hom Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts
0.172 1.38E-23 YMR070W MOT3 hom Transcriptional repressor and activator with two C2-H2 zinc fingers; involved in repression of a subset of hypoxic genes by Rox1p, repression of several DAN/TIR genes during aerobic growth, and repression of ergosterol biosynthetic genes in response to hyperosmotic stress; contributes to recruitment of the Tup1p-Cyc8p general repressor to promoters; involved in positive transcriptional regulation of CWP2 and other genes; can form the [MOT3+] prion
0.166 3.71E-22 YBR168W PEX32 hom Peroxisomal integral membrane protein, involved in negative regulation of peroxisome size; partially functionally redundant with Pex31p; genetic interactions suggest action at a step downstream of steps mediated by Pex28p and Pex29p
0.162 3.68E-21 YJL074C SMC3 het Subunit of the multiprotein cohesin complex required for sister chromatid cohesion in mitotic cells; also required, with Rec8p, for cohesion and recombination during meiosis; phylogenetically conserved SMC chromosomal ATPase family member
0.159 1.55E-20 YMR099C YMR099C hom Glucose-6-phosphate 1-epimerase (hexose-6-phosphate mutarotase), likely involved in carbohydrate metabolism; GFP-fusion protein localizes to both the nucleus and cytoplasm and is induced in response to the DNA-damaging agent MMS
0.158 2.75E-20 YPR064W_d YPR064W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.149 3.64E-18 YPR153W YPR153W hom Putative protein of unknown function