YML001W / YPT7

Rab family GTPase; GTP-binding protein of the rab family; required for homotypic fusion event in vacuole inheritance, for endosome-endosome fusion; interacts with the cargo selection/retromer complex for retrograde sorting; similar to mammalian Rab7

Zygosity: Homozygous strain
fixedexpanded
Profile for YML001W / YPT7

Click on Significant Values for Screen Details ID:SGTC_68|Compound:0986-0284|FD-Score:4.75|P-value:2.44E-7 ID:SGTC_69|Compound:0096-0274|FD-Score:5.18|P-value:2.01E-8 ID:SGTC_123|Compound:0083-0120|FD-Score:3.01|P-value:7.09E-4 ID:SGTC_127|Compound:0180-0333|FD-Score:3.43|P-value:1.39E-4 ID:SGTC_149|Compound:3970-0822|FD-Score:4.18|P-value:4.71E-6 ID:SGTC_178|Compound:4092-0806|FD-Score:-3.36|P-value:1.96E-4 ID:SGTC_243|Compound:5hr heat shock (37°C) + splitomicin|FD-Score:3.09|P-value:5.27E-4 ID:SGTC_245|Compound:methotrexate|FD-Score:5.13|P-value:2.78E-8 ID:SGTC_266|Compound:formosanin C|FD-Score:-3.23|P-value:3.35E-4 ID:SGTC_285|Compound:1486-1325|FD-Score:3.71|P-value:4.22E-5 ID:SGTC_308|Compound:4092-1169|FD-Score:3.71|P-value:4.20E-5 ID:SGTC_309|Compound:k408-0051|FD-Score:-4.17|P-value:5.41E-6 ID:SGTC_367|Compound:0335-0881|FD-Score:-3.48|P-value:1.19E-4 ID:SGTC_391|Compound:gliotoxin|FD-Score:10.90|P-value:7.10E-31 ID:SGTC_422|Compound:cantharidin|FD-Score:3.31|P-value:2.21E-4 ID:SGTC_431|Compound:k089-0097|FD-Score:4.67|P-value:3.68E-7 ID:SGTC_434|Compound:1-hexadecyl-2-methylglycero-3 PC|FD-Score:-3.00|P-value:7.72E-4 ID:SGTC_440|Compound:fluspirilene|FD-Score:3.47|P-value:1.17E-4 ID:SGTC_445|Compound:e6 berbamine|FD-Score:3.07|P-value:5.72E-4 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:5.14|P-value:2.65E-8 ID:SGTC_459|Compound:nigericin|FD-Score:6.16|P-value:3.59E-11 ID:SGTC_481|Compound:bay k-8644|FD-Score:3.04|P-value:6.33E-4 ID:SGTC_528|Compound:3492-0019|FD-Score:5.12|P-value:2.95E-8 ID:SGTC_576|Compound:1073-0113|FD-Score:3.81|P-value:2.66E-5 ID:SGTC_618|Compound:k072-0232|FD-Score:3.04|P-value:6.38E-4 ID:SGTC_699|Compound:0375-0592|FD-Score:3.47|P-value:1.16E-4 ID:SGTC_722|Compound:3534-0461|FD-Score:6.05|P-value:7.62E-11 ID:SGTC_841|Compound:0251-0215|FD-Score:5.41|P-value:4.97E-9 ID:SGTC_873|Compound:0591-4978|FD-Score:2.98|P-value:7.85E-4 ID:SGTC_895|Compound:0964-0006|FD-Score:3.91|P-value:1.70E-5 ID:SGTC_914|Compound:3820-6779|FD-Score:3.86|P-value:2.12E-5 ID:SGTC_1107|Compound:amlodipine|FD-Score:3.62|P-value:6.20E-5 ID:SGTC_1116|Compound:jasmonic acid|FD-Score:3.20|P-value:3.48E-4 ID:SGTC_1181|Compound:1222-0449|FD-Score:3.24|P-value:3.01E-4 ID:SGTC_1247|Compound:0448-0043|FD-Score:4.44|P-value:1.28E-6 ID:SGTC_1298|Compound:1079-1278|FD-Score:3.31|P-value:2.24E-4 ID:SGTC_1301|Compound:naphtanilide LB|FD-Score:3.03|P-value:6.61E-4 ID:SGTC_1327|Compound:1436-0082|FD-Score:-3.09|P-value:5.55E-4 ID:SGTC_1339|Compound:1486-1321|FD-Score:3.64|P-value:5.79E-5 ID:SGTC_1360|Compound:1598-0022|FD-Score:3.00|P-value:7.39E-4 ID:SGTC_1495|Compound:4469-0029|FD-Score:5.70|P-value:8.01E-10 ID:SGTC_1522|Compound:st012842|FD-Score:-3.21|P-value:3.52E-4 ID:SGTC_1593|Compound:justicidin b . 9-(1,3-benzodioxol-5-yl)- 6,7-dimethoxynaphtho[2,3-c]furan-1(3h)-one|FD-Score:3.00|P-value:7.34E-4 ID:SGTC_1595|Compound:6-ethoxy-3(4'-hydroxyphenyl)-4-methylcoumarin|FD-Score:3.78|P-value:3.03E-5 ID:SGTC_1658|Compound:st012944|FD-Score:4.68|P-value:3.58E-7 ID:SGTC_1687|Compound:st020778|FD-Score:14.60|P-value:4.97E-54 ID:SGTC_1728|Compound:st035614|FD-Score:-5.21|P-value:1.88E-8 ID:SGTC_1729|Compound:st035615|FD-Score:-4.16|P-value:5.75E-6 ID:SGTC_1750|Compound:st081859|FD-Score:6.50|P-value:2.97E-12 ID:SGTC_1800|Compound:st050079|FD-Score:3.78|P-value:3.12E-5 ID:SGTC_1844|Compound:rapamycin|FD-Score:5.52|P-value:2.60E-9 ID:SGTC_1874|Compound:staurosporine|FD-Score:3.20|P-value:3.41E-4 ID:SGTC_1877|Compound:wortmannin|FD-Score:12.30|P-value:2.94E-39 ID:SGTC_1917|Compound:st066507|FD-Score:3.12|P-value:4.65E-4 ID:SGTC_2029|Compound:5175081|FD-Score:3.62|P-value:6.22E-5 ID:SGTC_2046|Compound:4110841|FD-Score:2.99|P-value:7.63E-4 ID:SGTC_2200|Compound:6600604|FD-Score:3.00|P-value:7.45E-4 ID:SGTC_2514|Compound:salinomycin|FD-Score:3.63|P-value:5.90E-5 ID:SGTC_2619|Compound:obliquin|FD-Score:3.98|P-value:1.22E-5 ID:SGTC_2679|Compound:rapamycin|FD-Score:3.32|P-value:2.20E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:5.41|P-value:4.96E-9 ID:SGTC_2737|Compound:bleomycin|FD-Score:4.04|P-value:9.51E-6 ID:SGTC_2743|Compound:auranofin|FD-Score:4.26|P-value:3.22E-6 ID:SGTC_2820|Compound:7989630|FD-Score:3.95|P-value:1.41E-5 ID:SGTC_2837|Compound:9008476|FD-Score:-3.30|P-value:2.53E-4 ID:SGTC_3026|Compound:9090633|FD-Score:4.88|P-value:1.20E-7 ID:SGTC_3042|Compound:9091578|FD-Score:3.20|P-value:3.44E-4 ID:SGTC_3049|Compound:9092223|FD-Score:3.00|P-value:7.21E-4 ID:SGTC_3284|Compound:9108171|FD-Score:2.93|P-value:9.60E-4 ID:SGTC_3289|Compound:9119912|FD-Score:-3.24|P-value:3.21E-4 ID:SGTC_68|Compound:0986-0284|FD-Score:4.75|P-value:2.44E-7 ID:SGTC_69|Compound:0096-0274|FD-Score:5.18|P-value:2.01E-8 ID:SGTC_123|Compound:0083-0120|FD-Score:3.01|P-value:7.09E-4 ID:SGTC_127|Compound:0180-0333|FD-Score:3.43|P-value:1.39E-4 ID:SGTC_149|Compound:3970-0822|FD-Score:4.18|P-value:4.71E-6 ID:SGTC_178|Compound:4092-0806|FD-Score:-3.36|P-value:1.96E-4 ID:SGTC_243|Compound:5hr heat shock (37°C) + splitomicin|FD-Score:3.09|P-value:5.27E-4 ID:SGTC_245|Compound:methotrexate|FD-Score:5.13|P-value:2.78E-8 ID:SGTC_266|Compound:formosanin C|FD-Score:-3.23|P-value:3.35E-4 ID:SGTC_285|Compound:1486-1325|FD-Score:3.71|P-value:4.22E-5 ID:SGTC_308|Compound:4092-1169|FD-Score:3.71|P-value:4.20E-5 ID:SGTC_309|Compound:k408-0051|FD-Score:-4.17|P-value:5.41E-6 ID:SGTC_367|Compound:0335-0881|FD-Score:-3.48|P-value:1.19E-4 ID:SGTC_391|Compound:gliotoxin|FD-Score:10.90|P-value:7.10E-31 ID:SGTC_422|Compound:cantharidin|FD-Score:3.31|P-value:2.21E-4 ID:SGTC_431|Compound:k089-0097|FD-Score:4.67|P-value:3.68E-7 ID:SGTC_434|Compound:1-hexadecyl-2-methylglycero-3 PC|FD-Score:-3.00|P-value:7.72E-4 ID:SGTC_440|Compound:fluspirilene|FD-Score:3.47|P-value:1.17E-4 ID:SGTC_445|Compound:e6 berbamine|FD-Score:3.07|P-value:5.72E-4 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:5.14|P-value:2.65E-8 ID:SGTC_459|Compound:nigericin|FD-Score:6.16|P-value:3.59E-11 ID:SGTC_481|Compound:bay k-8644|FD-Score:3.04|P-value:6.33E-4 ID:SGTC_528|Compound:3492-0019|FD-Score:5.12|P-value:2.95E-8 ID:SGTC_576|Compound:1073-0113|FD-Score:3.81|P-value:2.66E-5 ID:SGTC_618|Compound:k072-0232|FD-Score:3.04|P-value:6.38E-4 ID:SGTC_699|Compound:0375-0592|FD-Score:3.47|P-value:1.16E-4 ID:SGTC_722|Compound:3534-0461|FD-Score:6.05|P-value:7.62E-11 ID:SGTC_841|Compound:0251-0215|FD-Score:5.41|P-value:4.97E-9 ID:SGTC_873|Compound:0591-4978|FD-Score:2.98|P-value:7.85E-4 ID:SGTC_895|Compound:0964-0006|FD-Score:3.91|P-value:1.70E-5 ID:SGTC_914|Compound:3820-6779|FD-Score:3.86|P-value:2.12E-5 ID:SGTC_1107|Compound:amlodipine|FD-Score:3.62|P-value:6.20E-5 ID:SGTC_1116|Compound:jasmonic acid|FD-Score:3.20|P-value:3.48E-4 ID:SGTC_1181|Compound:1222-0449|FD-Score:3.24|P-value:3.01E-4 ID:SGTC_1247|Compound:0448-0043|FD-Score:4.44|P-value:1.28E-6 ID:SGTC_1298|Compound:1079-1278|FD-Score:3.31|P-value:2.24E-4 ID:SGTC_1301|Compound:naphtanilide LB|FD-Score:3.03|P-value:6.61E-4 ID:SGTC_1327|Compound:1436-0082|FD-Score:-3.09|P-value:5.55E-4 ID:SGTC_1339|Compound:1486-1321|FD-Score:3.64|P-value:5.79E-5 ID:SGTC_1360|Compound:1598-0022|FD-Score:3.00|P-value:7.39E-4 ID:SGTC_1495|Compound:4469-0029|FD-Score:5.70|P-value:8.01E-10 ID:SGTC_1522|Compound:st012842|FD-Score:-3.21|P-value:3.52E-4 ID:SGTC_1593|Compound:justicidin b . 9-(1,3-benzodioxol-5-yl)- 6,7-dimethoxynaphtho[2,3-c]furan-1(3h)-one|FD-Score:3.00|P-value:7.34E-4 ID:SGTC_1595|Compound:6-ethoxy-3(4'-hydroxyphenyl)-4-methylcoumarin|FD-Score:3.78|P-value:3.03E-5 ID:SGTC_1658|Compound:st012944|FD-Score:4.68|P-value:3.58E-7 ID:SGTC_1687|Compound:st020778|FD-Score:14.60|P-value:4.97E-54 ID:SGTC_1728|Compound:st035614|FD-Score:-5.21|P-value:1.88E-8 ID:SGTC_1729|Compound:st035615|FD-Score:-4.16|P-value:5.75E-6 ID:SGTC_1750|Compound:st081859|FD-Score:6.50|P-value:2.97E-12 ID:SGTC_1800|Compound:st050079|FD-Score:3.78|P-value:3.12E-5 ID:SGTC_1844|Compound:rapamycin|FD-Score:5.52|P-value:2.60E-9 ID:SGTC_1874|Compound:staurosporine|FD-Score:3.20|P-value:3.41E-4 ID:SGTC_1877|Compound:wortmannin|FD-Score:12.30|P-value:2.94E-39 ID:SGTC_1917|Compound:st066507|FD-Score:3.12|P-value:4.65E-4 ID:SGTC_2029|Compound:5175081|FD-Score:3.62|P-value:6.22E-5 ID:SGTC_2046|Compound:4110841|FD-Score:2.99|P-value:7.63E-4 ID:SGTC_2200|Compound:6600604|FD-Score:3.00|P-value:7.45E-4 ID:SGTC_2514|Compound:salinomycin|FD-Score:3.63|P-value:5.90E-5 ID:SGTC_2619|Compound:obliquin|FD-Score:3.98|P-value:1.22E-5 ID:SGTC_2679|Compound:rapamycin|FD-Score:3.32|P-value:2.20E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:5.41|P-value:4.96E-9 ID:SGTC_2737|Compound:bleomycin|FD-Score:4.04|P-value:9.51E-6 ID:SGTC_2743|Compound:auranofin|FD-Score:4.26|P-value:3.22E-6 ID:SGTC_2820|Compound:7989630|FD-Score:3.95|P-value:1.41E-5 ID:SGTC_2837|Compound:9008476|FD-Score:-3.30|P-value:2.53E-4 ID:SGTC_3026|Compound:9090633|FD-Score:4.88|P-value:1.20E-7 ID:SGTC_3042|Compound:9091578|FD-Score:3.20|P-value:3.44E-4 ID:SGTC_3049|Compound:9092223|FD-Score:3.00|P-value:7.21E-4 ID:SGTC_3284|Compound:9108171|FD-Score:2.93|P-value:9.60E-4 ID:SGTC_3289|Compound:9119912|FD-Score:-3.24|P-value:3.21E-4

Top fitness defect scores for YML001W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1687 st020778 14.60 55.4 µM 4.97E-54 23 1.66E-48
2 SGTC_1877 wortmannin 12.30 25.0 µM 2.94E-39 6 2.73E-35
3 SGTC_391 gliotoxin 10.90 8.3 µM 7.10E-31 9 8.64E-28
4 SGTC_1750 st081859 6.50 2.4 µM 2.97E-12 endomembrane recycling 97 4.02E-11
5 SGTC_459 nigericin 6.16 15.3 µM 3.59E-11 30 3.74E-10
6 SGTC_722 3534-0461 6.05 190.0 µM 7.62E-11 PDR1 7 7.35E-10
7 SGTC_1495 4469-0029 5.70 237.0 µM 8.01E-10 60 6.05E-9
8 SGTC_1844 rapamycin 5.52 4.0 nM 2.60E-9 101 1.74E-8
9 SGTC_2688 kanamycin b 5.41 96.7 µM 4.96E-9 calcium & mitochondrial duress 202 3.10E-8
10 SGTC_841 0251-0215 5.41 91.2 µM 4.97E-9 47 3.10E-8
11 SGTC_69 0096-0274 5.18 206.7 µM 2.01E-8 mitochondrial processes 15 1.09E-7
12 SGTC_457 grayanotoxin iii 5.14 135.0 µM 2.65E-8 26 1.39E-7
13 SGTC_245 methotrexate 5.13 289.6 µM 2.78E-8 19 1.45E-7
14 SGTC_528 3492-0019 5.12 172.0 µM 2.95E-8 calcium & mitochondrial duress 152 1.53E-7
15 SGTC_3026 9090633 4.88 49.5 µM 1.20E-7 7 5.39E-7
16 SGTC_68 0986-0284 4.75 82.8 µM 2.44E-7 41 1.02E-6
17 SGTC_1658 st012944 4.68 20.0 µM 3.58E-7 iron homeostasis 54 1.43E-6
18 SGTC_431 k089-0097 4.67 107.0 µM 3.68E-7 iron homeostasis 19 1.47E-6
19 SGTC_1247 0448-0043 4.44 5.8 µM 1.28E-6 ERG2 26 4.49E-6
20 SGTC_2743 auranofin 4.26 4.3 µM 3.22E-6 RPP1 & pyrimidine depletion 28 1.03E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.557 5.82E-272 YGL212W VAM7 hom Vacuolar SNARE protein; functions with Vam3p in vacuolar protein trafficking; has an N-terminal PX domain (phosphoinositide-binding module) that binds PtdIns-3-P and mediates membrane binding; SNAP-25 homolog; protein abundance increases in response to DNA replication stress
0.543 5.70E-256 YDR080W VPS41 hom Vacuolar membrane protein that is a subunit of the homotypic vacuole fusion and vacuole protein sorting (HOPS) complex; essential for membrane docking and fusion at the Golgi-to-endosome and endosome-to-vacuole stages of protein transport
0.426 3.17E-148 YDL077C VAM6 hom Vacuolar protein that plays a critical role in the tethering steps of vacuolar membrane fusion by facilitating guanine nucleotide exchange on small guanosine triphosphatase Ypt7p
0.352 1.31E-98 YJL024C APS3 hom Small subunit of the clathrin-associated adaptor complex AP-3; involved in vacuolar protein sorting; related to the sigma subunit of the mammalian clathrin AP-3 complex; suppressor of loss of casein kinase 1 function; protein abundance increases in response to DNA replication stress
0.334 5.65E-88 YDL192W ARF1 hom ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated vesicle formation in intracellular trafficking within the Golgi; ARF1 has a paralog, ARF2, that arose from the whole genome duplication
0.319 4.80E-80 YGR261C APL6 hom Beta3-like subunit of the yeast AP-3 complex; functions in transport of alkaline phosphatase to the vacuole via the alternate pathway; exists in both cytosolic and peripherally associated membrane-bound pools
0.282 4.14E-62 YOR036W PEP12 hom Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin
0.279 4.77E-61 YBR288C APM3 hom Mu3-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway
0.278 2.08E-60 YBR131W CCZ1 hom Protein involved in vacuolar assembly, essential for autophagy and the cytoplasm-to-vacuole pathway
0.271 1.51E-57 YDR323C PEP7 hom Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance
0.271 1.70E-57 YPL195W APL5 hom Delta adaptin-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway, suppressor of loss of casein kinase 1 function
0.267 8.36E-56 YDR495C VPS3 hom Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase
0.263 2.80E-54 YJL164C TPK1 hom cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; inhibited by regulatory subunit Bcy1p in the absence of cAMP; regulates Whi3p; partially redundant with Tpk2p and Tpk3p; TPK1 has a paralog, TPK3, that arose from the whole genome duplication
0.256 2.27E-51 YGL124C MON1 hom Protein required for fusion of cvt-vesicles and autophagosomes with the vacuole; associates, as a complex with Ccz1p, with a perivacuolar compartment; potential Cdc28p substrate
0.256 3.51E-51 YJR033C RAV1 hom Subunit of the RAVE complex (Rav1p, Rav2p, Skp1p), which promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate