YML004C / GLO1

Monomeric glyoxalase I, catalyzes the detoxification of methylglyoxal (a by-product of glycolysis) via condensation with glutathione to produce S-D-lactoylglutathione; expression regulated by methylglyoxal levels and osmotic stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YML004C / GLO1

Click on Significant Values for Screen Details ID:SGTC_81|Compound:0411-0046|FD-Score:7.98|P-value:1.48E-15 ID:SGTC_182|Compound:3448-4371|FD-Score:-3.29|P-value:5.58E-4 ID:SGTC_265|Compound:mycophenolic acid|FD-Score:4.02|P-value:3.51E-5 ID:SGTC_362|Compound:0310-0100|FD-Score:-5.38|P-value:4.77E-8 ID:SGTC_500|Compound:h-89|FD-Score:3.29|P-value:5.73E-4 ID:SGTC_550|Compound:4123-0385|FD-Score:-3.54|P-value:2.23E-4 ID:SGTC_574|Compound:1315-0397|FD-Score:3.40|P-value:3.80E-4 ID:SGTC_690|Compound:glyoxal|FD-Score:3.34|P-value:4.79E-4 ID:SGTC_734|Compound:2023-0417|FD-Score:3.19|P-value:8.11E-4 ID:SGTC_917|Compound:harmaline|FD-Score:3.18|P-value:8.29E-4 ID:SGTC_918|Compound:glabranin|FD-Score:3.93|P-value:5.13E-5 ID:SGTC_996|Compound:3970-0799|FD-Score:5.91|P-value:2.43E-9 ID:SGTC_999|Compound:4022-0066|FD-Score:3.75|P-value:1.05E-4 ID:SGTC_1008|Compound:4106-0037|FD-Score:-3.54|P-value:2.28E-4 ID:SGTC_1019|Compound:4262-0212|FD-Score:3.53|P-value:2.42E-4 ID:SGTC_1036|Compound:k216-0215|FD-Score:4.50|P-value:4.17E-6 ID:SGTC_1045|Compound:1348-1485|FD-Score:3.20|P-value:7.81E-4 ID:SGTC_1252|Compound:0493-0875|FD-Score:5.57|P-value:1.74E-8 ID:SGTC_1331|Compound:1472-0049|FD-Score:3.42|P-value:3.59E-4 ID:SGTC_1445|Compound:4239-0156|FD-Score:3.59|P-value:1.94E-4 ID:SGTC_1459|Compound:k035-0031|FD-Score:5.46|P-value:3.32E-8 ID:SGTC_1464|Compound:k208-0027|FD-Score:-3.82|P-value:7.62E-5 ID:SGTC_1570|Compound:verbenone|FD-Score:-3.30|P-value:5.38E-4 ID:SGTC_1574|Compound:spiramycin|FD-Score:3.48|P-value:2.87E-4 ID:SGTC_1662|Compound:st014054|FD-Score:3.51|P-value:2.61E-4 ID:SGTC_1682|Compound:st012968|FD-Score:3.17|P-value:8.73E-4 ID:SGTC_1780|Compound:st038448|FD-Score:4.16|P-value:1.92E-5 ID:SGTC_1785|Compound:bathophenanthroline|FD-Score:3.69|P-value:1.31E-4 ID:SGTC_1814|Compound:st052110|FD-Score:6.58|P-value:3.80E-11 ID:SGTC_1847|Compound:st056231|FD-Score:6.14|P-value:6.22E-10 ID:SGTC_1873|Compound:rapamycin|FD-Score:3.31|P-value:5.27E-4 ID:SGTC_1955|Compound:wogonin|FD-Score:-3.21|P-value:7.20E-4 ID:SGTC_1972|Compound:st070201|FD-Score:3.88|P-value:6.25E-5 ID:SGTC_2260|Compound:7964312|FD-Score:3.36|P-value:4.39E-4 ID:SGTC_2362|Compound:9071156|FD-Score:4.08|P-value:2.78E-5 ID:SGTC_2413|Compound:inermin|FD-Score:4.97|P-value:4.32E-7 ID:SGTC_2441|Compound:5730946|FD-Score:-4.13|P-value:2.12E-5 ID:SGTC_2682|Compound:pyrogallin|FD-Score:3.29|P-value:5.73E-4 ID:SGTC_2690|Compound:usnic acid|FD-Score:3.46|P-value:3.11E-4 ID:SGTC_2797|Compound:7859098|FD-Score:4.50|P-value:4.22E-6 ID:SGTC_2831|Compound:7999036|FD-Score:4.56|P-value:3.23E-6 ID:SGTC_2832|Compound:7992762|FD-Score:3.99|P-value:3.94E-5 ID:SGTC_2844|Compound:9010103|FD-Score:4.12|P-value:2.32E-5 ID:SGTC_2951|Compound:9072950|FD-Score:-3.27|P-value:5.83E-4 ID:SGTC_3112|Compound:9123419|FD-Score:-3.23|P-value:6.90E-4 ID:SGTC_3116|Compound:9124943|FD-Score:4.62|P-value:2.39E-6 ID:SGTC_3216|Compound:9129716|FD-Score:6.64|P-value:2.58E-11 ID:SGTC_81|Compound:0411-0046|FD-Score:7.98|P-value:1.48E-15 ID:SGTC_182|Compound:3448-4371|FD-Score:-3.29|P-value:5.58E-4 ID:SGTC_265|Compound:mycophenolic acid|FD-Score:4.02|P-value:3.51E-5 ID:SGTC_362|Compound:0310-0100|FD-Score:-5.38|P-value:4.77E-8 ID:SGTC_500|Compound:h-89|FD-Score:3.29|P-value:5.73E-4 ID:SGTC_550|Compound:4123-0385|FD-Score:-3.54|P-value:2.23E-4 ID:SGTC_574|Compound:1315-0397|FD-Score:3.40|P-value:3.80E-4 ID:SGTC_690|Compound:glyoxal|FD-Score:3.34|P-value:4.79E-4 ID:SGTC_734|Compound:2023-0417|FD-Score:3.19|P-value:8.11E-4 ID:SGTC_917|Compound:harmaline|FD-Score:3.18|P-value:8.29E-4 ID:SGTC_918|Compound:glabranin|FD-Score:3.93|P-value:5.13E-5 ID:SGTC_996|Compound:3970-0799|FD-Score:5.91|P-value:2.43E-9 ID:SGTC_999|Compound:4022-0066|FD-Score:3.75|P-value:1.05E-4 ID:SGTC_1008|Compound:4106-0037|FD-Score:-3.54|P-value:2.28E-4 ID:SGTC_1019|Compound:4262-0212|FD-Score:3.53|P-value:2.42E-4 ID:SGTC_1036|Compound:k216-0215|FD-Score:4.50|P-value:4.17E-6 ID:SGTC_1045|Compound:1348-1485|FD-Score:3.20|P-value:7.81E-4 ID:SGTC_1252|Compound:0493-0875|FD-Score:5.57|P-value:1.74E-8 ID:SGTC_1331|Compound:1472-0049|FD-Score:3.42|P-value:3.59E-4 ID:SGTC_1445|Compound:4239-0156|FD-Score:3.59|P-value:1.94E-4 ID:SGTC_1459|Compound:k035-0031|FD-Score:5.46|P-value:3.32E-8 ID:SGTC_1464|Compound:k208-0027|FD-Score:-3.82|P-value:7.62E-5 ID:SGTC_1570|Compound:verbenone|FD-Score:-3.30|P-value:5.38E-4 ID:SGTC_1574|Compound:spiramycin|FD-Score:3.48|P-value:2.87E-4 ID:SGTC_1662|Compound:st014054|FD-Score:3.51|P-value:2.61E-4 ID:SGTC_1682|Compound:st012968|FD-Score:3.17|P-value:8.73E-4 ID:SGTC_1780|Compound:st038448|FD-Score:4.16|P-value:1.92E-5 ID:SGTC_1785|Compound:bathophenanthroline|FD-Score:3.69|P-value:1.31E-4 ID:SGTC_1814|Compound:st052110|FD-Score:6.58|P-value:3.80E-11 ID:SGTC_1847|Compound:st056231|FD-Score:6.14|P-value:6.22E-10 ID:SGTC_1873|Compound:rapamycin|FD-Score:3.31|P-value:5.27E-4 ID:SGTC_1955|Compound:wogonin|FD-Score:-3.21|P-value:7.20E-4 ID:SGTC_1972|Compound:st070201|FD-Score:3.88|P-value:6.25E-5 ID:SGTC_2260|Compound:7964312|FD-Score:3.36|P-value:4.39E-4 ID:SGTC_2362|Compound:9071156|FD-Score:4.08|P-value:2.78E-5 ID:SGTC_2413|Compound:inermin|FD-Score:4.97|P-value:4.32E-7 ID:SGTC_2441|Compound:5730946|FD-Score:-4.13|P-value:2.12E-5 ID:SGTC_2682|Compound:pyrogallin|FD-Score:3.29|P-value:5.73E-4 ID:SGTC_2690|Compound:usnic acid|FD-Score:3.46|P-value:3.11E-4 ID:SGTC_2797|Compound:7859098|FD-Score:4.50|P-value:4.22E-6 ID:SGTC_2831|Compound:7999036|FD-Score:4.56|P-value:3.23E-6 ID:SGTC_2832|Compound:7992762|FD-Score:3.99|P-value:3.94E-5 ID:SGTC_2844|Compound:9010103|FD-Score:4.12|P-value:2.32E-5 ID:SGTC_2951|Compound:9072950|FD-Score:-3.27|P-value:5.83E-4 ID:SGTC_3112|Compound:9123419|FD-Score:-3.23|P-value:6.90E-4 ID:SGTC_3116|Compound:9124943|FD-Score:4.62|P-value:2.39E-6 ID:SGTC_3216|Compound:9129716|FD-Score:6.64|P-value:2.58E-11

Top fitness defect scores for YML004C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_81 0411-0046 7.98 10.0 µM 1.48E-15 4 7.61E-16
2 SGTC_3216 9129716 6.64 49.5 µM 2.58E-11 RPP1 & pyrimidine depletion 3 1.62E-11
3 SGTC_1814 st052110 6.58 31.5 µM 3.80E-11 RNA processing & uracil transport 2 2.40E-11
4 SGTC_1847 st056231 6.14 15.0 µM 6.22E-10 5 4.16E-10
5 SGTC_996 3970-0799 5.91 1.8 µM 2.43E-9 37 1.67E-9
6 SGTC_1252 0493-0875 5.57 34.8 µM 1.74E-8 7 1.24E-8
7 SGTC_1459 k035-0031 5.46 35.7 µM 3.32E-8 DNA intercalators 6 2.40E-8
8 SGTC_2413 inermin 4.97 26.1 µM 4.32E-7 30 3.29E-7
9 SGTC_3116 9124943 4.62 49.5 µM 2.39E-6 8 1.88E-6
10 SGTC_2831 7999036 4.56 19.5 µM 3.23E-6 27 2.56E-6
11 SGTC_1036 k216-0215 4.50 33.5 µM 4.17E-6 mitochondrial response to ROS 35 3.33E-6
12 SGTC_2797 7859098 4.50 81.8 µM 4.22E-6 19 3.36E-6
13 SGTC_1780 st038448 4.16 24.5 µM 1.92E-5 iron homeostasis 12 1.58E-5
14 SGTC_2844 9010103 4.12 26.0 µM 2.32E-5 31 1.91E-5
15 SGTC_2362 9071156 4.08 126.0 µM 2.78E-5 7 2.30E-5
16 SGTC_265 mycophenolic acid 4.02 32.9 µM 3.51E-5 RPP1 & pyrimidine depletion 15 2.92E-5
17 SGTC_2832 7992762 3.99 52.0 µM 3.94E-5 30 3.28E-5
18 SGTC_918 glabranin 3.93 770.7 nM 5.13E-5 33 4.30E-5
19 SGTC_1972 st070201 3.88 17.4 µM 6.25E-5 25 5.25E-5
20 SGTC_999 4022-0066 3.75 22.7 µM 1.05E-4 Golgi 55 8.89E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.177 4.29E-25 YFL038C YPT1 het Rab family GTPase, involved in the ER-to-Golgi step of the secretory pathway; complex formation with the Rab escort protein Mrs6p is required for prenylation of Ypt1p by protein geranylgeranyltransferase type II (Bet2p-Bet4p)
0.175 1.40E-24 YML118W NGL3 hom 3'-5' exonuclease specific for poly-A RNAs; has a domain similar to a magnesium-dependent endonuclease motif in mRNA deadenylase Ccr4p; similar to Ngl1p; NGL3 has a paralog, NGL2, that arose from the whole genome duplication
0.142 1.34E-16 YOL146W PSF3 het Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery
0.139 5.00E-16 YDL099W BUG1 hom Cis-golgi localized protein involved in ER to Golgi transport; forms a complex with the mammalian GRASP65 homolog, Grh1p; mutants are compromised for the fusion of ER-derived vesicles with Golgi membranes
0.134 6.15E-15 YOR037W CYC2 hom Mitochondrial peripheral inner membrane protein, contains a FAD cofactor in a domain exposed in the intermembrane space; exhibits redox activity in vitro; likely participates in ligation of heme to acytochromes c and c1 (Cyc1p and Cyt1p)
0.131 2.20E-14 YGL040C HEM2 het Aminolevulinate dehydratase, a homo-octameric enzyme, catalyzes the conversion of 5-aminolevulinate to porphobilinogen, the second step in heme biosynthesis; enzymatic activity is zinc-dependent; localizes to the cytoplasm and nucleus
0.130 4.82E-14 YDR041W RSM10 het Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S10 ribosomal protein; essential for viability, unlike most other mitoribosomal proteins
0.125 3.40E-13 YDR272W GLO2 hom Cytoplasmic glyoxalase II, catalyzes the hydrolysis of S-D-lactoylglutathione into glutathione and D-lactate
0.125 3.94E-13 YDR505C PSP1 hom Asn and gln rich protein of unknown function; high-copy suppressor of POL1 (DNA polymerase alpha) and partial suppressor of CDC2 (polymerase delta) and CDC6 (pre-RC loading factor) mutations; overexpression results in growth inhibition; PSP1 has a paralog, YLR177W, that arose from the whole genome duplication
0.116 1.78E-11 YBR067C TIP1 hom Major cell wall mannoprotein with possible lipase activity; transcription is induced by heat- and cold-shock; member of the Srp1p/Tip1p family of serine-alanine-rich proteins
0.115 2.34E-11 YDR189W SLY1 het Hydrophilic protein involved in vesicle trafficking between the ER and Golgi; SM (Sec1/Munc-18) family protein that binds the tSNARE Sed5p and stimulates its assembly into a trans-SNARE membrane-protein complex
0.113 5.16E-11 YGL222C EDC1 hom RNA-binding protein that activates mRNA decapping directly; binds to the mRNA substrate and enhances the activity of the decapping proteins Dcp1p and Dcp2p; has a role in translation during heat stress; protein becomes more abundant and forms cytoplasmic foci in response to DNA replication stress
0.109 2.27E-10 YDL092W SRP14 het Signal recognition particle (SRP) subunit, interacts with the RNA component of SRP to form the Alu domain, which is the region of SRP responsible for arrest of nascent chain elongation during membrane targeting; homolog of mammalian SRP14
0.107 5.44E-10 YGL205W POX1 hom Fatty-acyl coenzyme A oxidase, involved in the fatty acid beta-oxidation pathway; localized to the peroxisomal matrix
0.105 9.69E-10 YMR260C TIF11 het Translation initiation factor eIF1A, essential protein that forms a complex with Sui1p (eIF1) and the 40S ribosomal subunit and scans for the start codon; C-terminus associates with Fun12p (eIF5B); N terminus interacts with eIF2 and eIF3