YML019W / OST6

Subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; similar to and partially functionally redundant with Ost3p

Zygosity: Homozygous strain
fixedexpanded
Profile for YML019W / OST6

Click on Significant Values for Screen Details ID:SGTC_53|Compound:0973-0023|FD-Score:5.82|P-value:7.54E-9 ID:SGTC_58|Compound:0957-0393|FD-Score:-7.38|P-value:2.33E-13 ID:SGTC_223|Compound:busulfan|FD-Score:3.23|P-value:8.89E-4 ID:SGTC_304|Compound:1310-0298|FD-Score:-3.55|P-value:2.40E-4 ID:SGTC_351|Compound:caspofungin|FD-Score:3.79|P-value:1.19E-4 ID:SGTC_353|Compound:1988-1175|FD-Score:4.84|P-value:1.29E-6 ID:SGTC_455|Compound:paf C-16|FD-Score:4.41|P-value:9.42E-6 ID:SGTC_582|Compound:0987-0078|FD-Score:3.37|P-value:5.52E-4 ID:SGTC_602|Compound:3130-1533|FD-Score:3.26|P-value:8.08E-4 ID:SGTC_692|Compound:k241-0027|FD-Score:-3.25|P-value:6.82E-4 ID:SGTC_705|Compound:1124-0212|FD-Score:3.25|P-value:8.36E-4 ID:SGTC_757|Compound:1066-0008|FD-Score:3.48|P-value:3.71E-4 ID:SGTC_821|Compound:1114-0266|FD-Score:3.68|P-value:1.84E-4 ID:SGTC_822|Compound:1487-0950|FD-Score:-3.30|P-value:5.74E-4 ID:SGTC_831|Compound:k061-1017|FD-Score:-3.20|P-value:8.01E-4 ID:SGTC_842|Compound:0271-0008|FD-Score:-3.23|P-value:7.20E-4 ID:SGTC_960|Compound:1222-0186|FD-Score:3.26|P-value:7.90E-4 ID:SGTC_964|Compound:1287-0107|FD-Score:5.24|P-value:1.78E-7 ID:SGTC_1067|Compound:clotrimazole|FD-Score:3.81|P-value:1.09E-4 ID:SGTC_1068|Compound:loratadine|FD-Score:3.55|P-value:2.88E-4 ID:SGTC_1078|Compound:idebenone|FD-Score:4.31|P-value:1.47E-5 ID:SGTC_1087|Compound:pterostilbene|FD-Score:4.70|P-value:2.46E-6 ID:SGTC_1092|Compound:toremifene|FD-Score:3.49|P-value:3.61E-4 ID:SGTC_1203|Compound:3992-3522|FD-Score:4.01|P-value:4.94E-5 ID:SGTC_1235|Compound:benzylparaben|FD-Score:3.55|P-value:2.88E-4 ID:SGTC_1258|Compound:0671-0043|FD-Score:3.88|P-value:8.41E-5 ID:SGTC_1296|Compound:1068-0102|FD-Score:5.10|P-value:3.62E-7 ID:SGTC_1479|Compound:0549-0158|FD-Score:3.73|P-value:1.48E-4 ID:SGTC_1509|Compound:1326-1131|FD-Score:3.69|P-value:1.74E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:3.56|P-value:2.85E-4 ID:SGTC_1623|Compound:st003709|FD-Score:3.51|P-value:3.34E-4 ID:SGTC_1869|Compound:3',7'-dimethoxyflavone|FD-Score:3.92|P-value:7.24E-5 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:-3.33|P-value:5.22E-4 ID:SGTC_2006|Compound:4011487|FD-Score:3.73|P-value:1.47E-4 ID:SGTC_2007|Compound:4011539|FD-Score:3.46|P-value:4.00E-4 ID:SGTC_2009|Compound:4012297|FD-Score:5.62|P-value:2.40E-8 ID:SGTC_2084|Compound:5306078|FD-Score:-3.41|P-value:3.90E-4 ID:SGTC_2091|Compound:4-phenylthiadiazole|FD-Score:4.35|P-value:1.22E-5 ID:SGTC_2528|Compound:xanthyletin|FD-Score:3.35|P-value:5.79E-4 ID:SGTC_2531|Compound:methyl deoxycholate|FD-Score:-4.00|P-value:4.26E-5 ID:SGTC_2534|Compound:helenine|FD-Score:4.30|P-value:1.49E-5 ID:SGTC_2601|Compound:securinine|FD-Score:3.50|P-value:3.45E-4 ID:SGTC_2669|Compound:eugenol|FD-Score:-3.55|P-value:2.40E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:9.15|P-value:2.47E-19 ID:SGTC_2786|Compound:7743617|FD-Score:-4.03|P-value:3.72E-5 ID:SGTC_2956|Compound:9076196|FD-Score:3.34|P-value:5.98E-4 ID:SGTC_2979|Compound:9012552|FD-Score:4.64|P-value:3.27E-6 ID:SGTC_2984|Compound:9022109|FD-Score:3.74|P-value:1.42E-4 ID:SGTC_3275|Compound:9138069|FD-Score:3.36|P-value:5.65E-4 ID:SGTC_3295|Compound:9122362|FD-Score:3.23|P-value:8.77E-4 ID:SGTC_3297|Compound:9117502|FD-Score:-3.16|P-value:9.30E-4 ID:SGTC_3346|Compound:9158254|FD-Score:-3.38|P-value:4.39E-4 ID:SGTC_53|Compound:0973-0023|FD-Score:5.82|P-value:7.54E-9 ID:SGTC_58|Compound:0957-0393|FD-Score:-7.38|P-value:2.33E-13 ID:SGTC_223|Compound:busulfan|FD-Score:3.23|P-value:8.89E-4 ID:SGTC_304|Compound:1310-0298|FD-Score:-3.55|P-value:2.40E-4 ID:SGTC_351|Compound:caspofungin|FD-Score:3.79|P-value:1.19E-4 ID:SGTC_353|Compound:1988-1175|FD-Score:4.84|P-value:1.29E-6 ID:SGTC_455|Compound:paf C-16|FD-Score:4.41|P-value:9.42E-6 ID:SGTC_582|Compound:0987-0078|FD-Score:3.37|P-value:5.52E-4 ID:SGTC_602|Compound:3130-1533|FD-Score:3.26|P-value:8.08E-4 ID:SGTC_692|Compound:k241-0027|FD-Score:-3.25|P-value:6.82E-4 ID:SGTC_705|Compound:1124-0212|FD-Score:3.25|P-value:8.36E-4 ID:SGTC_757|Compound:1066-0008|FD-Score:3.48|P-value:3.71E-4 ID:SGTC_821|Compound:1114-0266|FD-Score:3.68|P-value:1.84E-4 ID:SGTC_822|Compound:1487-0950|FD-Score:-3.30|P-value:5.74E-4 ID:SGTC_831|Compound:k061-1017|FD-Score:-3.20|P-value:8.01E-4 ID:SGTC_842|Compound:0271-0008|FD-Score:-3.23|P-value:7.20E-4 ID:SGTC_960|Compound:1222-0186|FD-Score:3.26|P-value:7.90E-4 ID:SGTC_964|Compound:1287-0107|FD-Score:5.24|P-value:1.78E-7 ID:SGTC_1067|Compound:clotrimazole|FD-Score:3.81|P-value:1.09E-4 ID:SGTC_1068|Compound:loratadine|FD-Score:3.55|P-value:2.88E-4 ID:SGTC_1078|Compound:idebenone|FD-Score:4.31|P-value:1.47E-5 ID:SGTC_1087|Compound:pterostilbene|FD-Score:4.70|P-value:2.46E-6 ID:SGTC_1092|Compound:toremifene|FD-Score:3.49|P-value:3.61E-4 ID:SGTC_1203|Compound:3992-3522|FD-Score:4.01|P-value:4.94E-5 ID:SGTC_1235|Compound:benzylparaben|FD-Score:3.55|P-value:2.88E-4 ID:SGTC_1258|Compound:0671-0043|FD-Score:3.88|P-value:8.41E-5 ID:SGTC_1296|Compound:1068-0102|FD-Score:5.10|P-value:3.62E-7 ID:SGTC_1479|Compound:0549-0158|FD-Score:3.73|P-value:1.48E-4 ID:SGTC_1509|Compound:1326-1131|FD-Score:3.69|P-value:1.74E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:3.56|P-value:2.85E-4 ID:SGTC_1623|Compound:st003709|FD-Score:3.51|P-value:3.34E-4 ID:SGTC_1869|Compound:3',7'-dimethoxyflavone|FD-Score:3.92|P-value:7.24E-5 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:-3.33|P-value:5.22E-4 ID:SGTC_2006|Compound:4011487|FD-Score:3.73|P-value:1.47E-4 ID:SGTC_2007|Compound:4011539|FD-Score:3.46|P-value:4.00E-4 ID:SGTC_2009|Compound:4012297|FD-Score:5.62|P-value:2.40E-8 ID:SGTC_2084|Compound:5306078|FD-Score:-3.41|P-value:3.90E-4 ID:SGTC_2091|Compound:4-phenylthiadiazole|FD-Score:4.35|P-value:1.22E-5 ID:SGTC_2528|Compound:xanthyletin|FD-Score:3.35|P-value:5.79E-4 ID:SGTC_2531|Compound:methyl deoxycholate|FD-Score:-4.00|P-value:4.26E-5 ID:SGTC_2534|Compound:helenine|FD-Score:4.30|P-value:1.49E-5 ID:SGTC_2601|Compound:securinine|FD-Score:3.50|P-value:3.45E-4 ID:SGTC_2669|Compound:eugenol|FD-Score:-3.55|P-value:2.40E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:9.15|P-value:2.47E-19 ID:SGTC_2786|Compound:7743617|FD-Score:-4.03|P-value:3.72E-5 ID:SGTC_2956|Compound:9076196|FD-Score:3.34|P-value:5.98E-4 ID:SGTC_2979|Compound:9012552|FD-Score:4.64|P-value:3.27E-6 ID:SGTC_2984|Compound:9022109|FD-Score:3.74|P-value:1.42E-4 ID:SGTC_3275|Compound:9138069|FD-Score:3.36|P-value:5.65E-4 ID:SGTC_3295|Compound:9122362|FD-Score:3.23|P-value:8.77E-4 ID:SGTC_3297|Compound:9117502|FD-Score:-3.16|P-value:9.30E-4 ID:SGTC_3346|Compound:9158254|FD-Score:-3.38|P-value:4.39E-4

Top fitness defect scores for YML019W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2688 kanamycin b 9.15 96.7 µM 2.47E-19 calcium & mitochondrial duress 41 2.76E-20
2 SGTC_53 0973-0023 5.82 40.0 µM 7.54E-9 RNA processing & uracil transport 3 2.89E-9
3 SGTC_2009 4012297 5.62 12.1 µM 2.40E-8 cell wall signaling 27 9.74E-9
4 SGTC_964 1287-0107 5.24 19.4 µM 1.78E-7 cell wall 19 8.02E-8
5 SGTC_1296 1068-0102 5.10 11.9 µM 3.62E-7 3 1.70E-7
6 SGTC_353 1988-1175 4.84 58.3 µM 1.29E-6 unfolded protein response 13 6.45E-7
7 SGTC_1087 pterostilbene 4.70 9.1 µM 2.46E-6 11 1.27E-6
8 SGTC_2979 9012552 4.64 45.5 µM 3.27E-6 4 1.72E-6
9 SGTC_455 paf C-16 4.41 10.0 µM 9.42E-6 24 5.22E-6
10 SGTC_2091 4-phenylthiadiazole 4.35 200.0 µM 1.22E-5 8 6.85E-6
11 SGTC_1078 idebenone 4.31 36.1 µM 1.47E-5 Golgi 32 8.35E-6
12 SGTC_2534 helenine 4.30 20.1 µM 1.49E-5 RSC complex & mRNA processing 10 8.44E-6
13 SGTC_1203 3992-3522 4.01 103.0 µM 4.94E-5 27 2.99E-5
14 SGTC_1869 3',7'-dimethoxyflavone 3.92 40.3 µM 7.24E-5 27 4.47E-5
15 SGTC_1258 0671-0043 3.88 57.1 µM 8.41E-5 26 5.23E-5
16 SGTC_1067 clotrimazole 3.81 1.4 µM 1.09E-4 azole & statin 46 6.87E-5
17 SGTC_351 caspofungin 3.79 25.0 nM 1.19E-4 cell wall 106 7.55E-5
18 SGTC_2984 9022109 3.74 39.0 µM 1.42E-4 9 9.05E-5
19 SGTC_2006 4011487 3.73 200.0 µM 1.47E-4 endomembrane recycling 89 9.41E-5
20 SGTC_1479 0549-0158 3.73 134.0 µM 1.48E-4 34 9.49E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.192 2.44E-29 YDR101C ARX1 hom Shuttling pre-60S factor; involved in the biogenesis of ribosomal large subunit biogenesis; interacts directly with Alb1; responsible for Tif6 recycling defects in absence of Rei1; associated with the ribosomal export complex
0.181 2.94E-26 YNL219C ALG9 hom Mannosyltransferase, involved in N-linked glycosylation; catalyzes both the transfer of seventh mannose residue on B-arm and ninth mannose residue on the C-arm from Dol-P-Man to lipid-linked oligosaccharides; mutation of the human ortholog causes type 1 congenital disorders of glycosylation
0.164 1.21E-21 YOR002W ALG6 hom Alpha 1,3 glucosyltransferase, involved in transfer of oligosaccharides from dolichyl pyrophosphate to asparagine residues of proteins during N-linked protein glycosylation; mutations in human ortholog are associated with disease
0.163 1.82E-21 YML115C VAN1 hom Component of the mannan polymerase I, which contains Van1p and Mnn9p and is involved in the first steps of mannan synthesis; mutants are vanadate-resistant
0.156 1.31E-19 YDR162C NBP2 hom Protein involved in the HOG (high osmolarity glycerol) pathway; negatively regulates Hog1p by recruitment of phosphatase Ptc1p the Pbs2p-Hog1p complex; interacts with Bck1p and down regulates the cell wall integrity pathway; found in the nucleus and cytoplasm, contains an SH3 domain and a Ptc1p binding domain (PBM)
0.155 1.48E-19 YGR166W TRS65 hom Subunit of TRAPPII, a multimeric guanine nucleotide-exchange factor for Ypt1p; involved in intra-Golgi traffic and the retrograde pathway from the endosome to Golgi; role in cell wall beta-glucan biosynthesis and the stress response
0.153 5.38E-19 YMR099C YMR099C hom Glucose-6-phosphate 1-epimerase (hexose-6-phosphate mutarotase), likely involved in carbohydrate metabolism; GFP-fusion protein localizes to both the nucleus and cytoplasm and is induced in response to the DNA-damaging agent MMS
0.145 3.00E-17 YDR156W RPA14 hom RNA polymerase I subunit A14
0.144 5.37E-17 YJR075W HOC1 hom Alpha-1,6-mannosyltransferase involved in cell wall mannan biosynthesis; subunit of a Golgi-localized complex that also contains Anp1p, Mnn9p, Mnn11p, and Mnn10p; identified as a suppressor of a cell lysis sensitive pkc1-371 allele
0.144 5.81E-17 YBL082C ALG3 hom Dolichol-P-Man dependent alpha(1-3) mannosyltransferase, involved in the synthesis of dolichol-linked oligosaccharide donor for N-linked glycosylation of proteins
0.142 1.48E-16 YGR206W MVB12 hom ESCRT-I subunit required to stabilize oligomers of the ESCRT-I core complex (Stp22p, Vps28p, Srn2p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; deletion mutant is sensitive to rapamycin and nystatin
0.138 9.99E-16 YPL227C ALG5 hom UDP-glucose:dolichyl-phosphate glucosyltransferase, involved in asparagine-linked glycosylation in the endoplasmic reticulum
0.136 2.53E-15 YDR467C_d YDR467C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.136 2.72E-15 YDR525W_d API2_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 26% of ORF overlaps the dubious ORF YDR524C-A; insertion mutation in a cdc34-2 mutant background causes altered bud morphology
0.135 4.00E-15 YNL067W RPL9B hom Ribosomal 60S subunit protein L9B; nearly identical to paralog Rpl9Ap; homologous to mammalian ribosomal protein L9 and bacterial L6