YML029W / USA1

Scaffold subunit of the Hrd1p ubiquitin ligase that also promotes ligase oligomerization; involved in ER-associated protein degradation (ERAD); interacts with the U1 snRNP-specific protein, Snp1p

Zygosity: Homozygous strain
fixedexpanded
Profile for YML029W / USA1

Click on Significant Values for Screen Details ID:SGTC_12|Compound:0849-0099|FD-Score:4.84|P-value:1.86E-7 ID:SGTC_44|Compound:0929-0055|FD-Score:-5.59|P-value:2.74E-9 ID:SGTC_86|Compound:0417-1630|FD-Score:3.10|P-value:5.56E-4 ID:SGTC_158|Compound:k038-0087|FD-Score:3.30|P-value:2.65E-4 ID:SGTC_169|Compound:k015-0017|FD-Score:2.99|P-value:8.41E-4 ID:SGTC_216|Compound:k038-0035|FD-Score:3.88|P-value:2.30E-5 ID:SGTC_266|Compound:formosanin C|FD-Score:3.51|P-value:1.14E-4 ID:SGTC_276|Compound:colchicine|FD-Score:2.95|P-value:9.78E-4 ID:SGTC_392|Compound:N-ethylmaleimide|FD-Score:3.49|P-value:1.23E-4 ID:SGTC_425|Compound:3825-7017|FD-Score:3.87|P-value:2.43E-5 ID:SGTC_513|Compound:4245-1575|FD-Score:5.92|P-value:2.68E-10 ID:SGTC_516|Compound:3992-2719|FD-Score:-3.46|P-value:1.57E-4 ID:SGTC_702|Compound:1488-0190|FD-Score:3.46|P-value:1.39E-4 ID:SGTC_758|Compound:0868-0029|FD-Score:-12.60|P-value:1.15E-39 ID:SGTC_931|Compound:2812-0076|FD-Score:-3.92|P-value:2.25E-5 ID:SGTC_1016|Compound:4226-1401|FD-Score:-3.36|P-value:2.29E-4 ID:SGTC_1069|Compound:diphenylcyclopropenone|FD-Score:3.48|P-value:1.29E-4 ID:SGTC_1238|Compound:0333-0287|FD-Score:-3.20|P-value:4.31E-4 ID:SGTC_1268|Compound:0828-0275|FD-Score:-3.64|P-value:7.55E-5 ID:SGTC_1445|Compound:4239-0156|FD-Score:3.23|P-value:3.41E-4 ID:SGTC_1600|Compound:coumarin 106|FD-Score:3.03|P-value:7.27E-4 ID:SGTC_1712|Compound:st032288|FD-Score:-3.05|P-value:7.56E-4 ID:SGTC_1714|Compound:st033236|FD-Score:3.01|P-value:7.70E-4 ID:SGTC_1729|Compound:st035615|FD-Score:-3.92|P-value:2.19E-5 ID:SGTC_1892|Compound:st058411|FD-Score:3.29|P-value:2.79E-4 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:-3.34|P-value:2.48E-4 ID:SGTC_1945|Compound:st075991|FD-Score:-3.17|P-value:4.81E-4 ID:SGTC_2288|Compound:7946289|FD-Score:3.41|P-value:1.72E-4 ID:SGTC_2336|Compound:9001819|FD-Score:3.12|P-value:5.19E-4 ID:SGTC_2614|Compound:arthonioic acid|FD-Score:-3.62|P-value:8.15E-5 ID:SGTC_2631|Compound:bromo-3-hydroxy-4-(succin-2-yl)-caryolane gamma-lactone|FD-Score:5.03|P-value:6.57E-8 ID:SGTC_2640|Compound:pentamidine|FD-Score:4.11|P-value:7.92E-6 ID:SGTC_2651|Compound:1327-0036|FD-Score:3.59|P-value:8.10E-5 ID:SGTC_2701|Compound:st077767|FD-Score:5.49|P-value:4.27E-9 ID:SGTC_2787|Compound:5284540|FD-Score:3.75|P-value:4.06E-5 ID:SGTC_2823|Compound:7987624|FD-Score:-4.37|P-value:2.66E-6 ID:SGTC_2887|Compound:9044784|FD-Score:3.29|P-value:2.73E-4 ID:SGTC_2941|Compound:9056145|FD-Score:3.26|P-value:3.07E-4 ID:SGTC_3162|Compound:9099653|FD-Score:3.01|P-value:7.86E-4 ID:SGTC_12|Compound:0849-0099|FD-Score:4.84|P-value:1.86E-7 ID:SGTC_44|Compound:0929-0055|FD-Score:-5.59|P-value:2.74E-9 ID:SGTC_86|Compound:0417-1630|FD-Score:3.10|P-value:5.56E-4 ID:SGTC_158|Compound:k038-0087|FD-Score:3.30|P-value:2.65E-4 ID:SGTC_169|Compound:k015-0017|FD-Score:2.99|P-value:8.41E-4 ID:SGTC_216|Compound:k038-0035|FD-Score:3.88|P-value:2.30E-5 ID:SGTC_266|Compound:formosanin C|FD-Score:3.51|P-value:1.14E-4 ID:SGTC_276|Compound:colchicine|FD-Score:2.95|P-value:9.78E-4 ID:SGTC_392|Compound:N-ethylmaleimide|FD-Score:3.49|P-value:1.23E-4 ID:SGTC_425|Compound:3825-7017|FD-Score:3.87|P-value:2.43E-5 ID:SGTC_513|Compound:4245-1575|FD-Score:5.92|P-value:2.68E-10 ID:SGTC_516|Compound:3992-2719|FD-Score:-3.46|P-value:1.57E-4 ID:SGTC_702|Compound:1488-0190|FD-Score:3.46|P-value:1.39E-4 ID:SGTC_758|Compound:0868-0029|FD-Score:-12.60|P-value:1.15E-39 ID:SGTC_931|Compound:2812-0076|FD-Score:-3.92|P-value:2.25E-5 ID:SGTC_1016|Compound:4226-1401|FD-Score:-3.36|P-value:2.29E-4 ID:SGTC_1069|Compound:diphenylcyclopropenone|FD-Score:3.48|P-value:1.29E-4 ID:SGTC_1238|Compound:0333-0287|FD-Score:-3.20|P-value:4.31E-4 ID:SGTC_1268|Compound:0828-0275|FD-Score:-3.64|P-value:7.55E-5 ID:SGTC_1445|Compound:4239-0156|FD-Score:3.23|P-value:3.41E-4 ID:SGTC_1600|Compound:coumarin 106|FD-Score:3.03|P-value:7.27E-4 ID:SGTC_1712|Compound:st032288|FD-Score:-3.05|P-value:7.56E-4 ID:SGTC_1714|Compound:st033236|FD-Score:3.01|P-value:7.70E-4 ID:SGTC_1729|Compound:st035615|FD-Score:-3.92|P-value:2.19E-5 ID:SGTC_1892|Compound:st058411|FD-Score:3.29|P-value:2.79E-4 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:-3.34|P-value:2.48E-4 ID:SGTC_1945|Compound:st075991|FD-Score:-3.17|P-value:4.81E-4 ID:SGTC_2288|Compound:7946289|FD-Score:3.41|P-value:1.72E-4 ID:SGTC_2336|Compound:9001819|FD-Score:3.12|P-value:5.19E-4 ID:SGTC_2614|Compound:arthonioic acid|FD-Score:-3.62|P-value:8.15E-5 ID:SGTC_2631|Compound:bromo-3-hydroxy-4-(succin-2-yl)-caryolane gamma-lactone|FD-Score:5.03|P-value:6.57E-8 ID:SGTC_2640|Compound:pentamidine|FD-Score:4.11|P-value:7.92E-6 ID:SGTC_2651|Compound:1327-0036|FD-Score:3.59|P-value:8.10E-5 ID:SGTC_2701|Compound:st077767|FD-Score:5.49|P-value:4.27E-9 ID:SGTC_2787|Compound:5284540|FD-Score:3.75|P-value:4.06E-5 ID:SGTC_2823|Compound:7987624|FD-Score:-4.37|P-value:2.66E-6 ID:SGTC_2887|Compound:9044784|FD-Score:3.29|P-value:2.73E-4 ID:SGTC_2941|Compound:9056145|FD-Score:3.26|P-value:3.07E-4 ID:SGTC_3162|Compound:9099653|FD-Score:3.01|P-value:7.86E-4

Top fitness defect scores for YML029W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_513 4245-1575 5.92 41.7 µM 2.68E-10 unfolded protein response 49 1.60E-9
2 SGTC_2701 st077767 5.49 14.7 µM 4.27E-9 19 2.01E-8
3 SGTC_2631 bromo-3-hydroxy-4-(succin-2-yl)-caryolane gamma-lactone 5.03 100.0 µM 6.57E-8 17 2.45E-7
4 SGTC_12 0849-0099 4.84 9.3 µM 1.86E-7 12 6.34E-7
5 SGTC_2640 pentamidine 4.11 10.4 µM 7.92E-6 mitochondrial processes 29 1.96E-5
6 SGTC_216 k038-0035 3.88 28.4 µM 2.30E-5 ubiquinone biosynthesis & proteasome 49 5.19E-5
7 SGTC_425 3825-7017 3.87 13.1 µM 2.43E-5 23 5.46E-5
8 SGTC_2787 5284540 3.75 71.4 µM 4.06E-5 30 8.74E-5
9 SGTC_2651 1327-0036 3.59 5.0 µM 8.10E-5 187 1.64E-4
10 SGTC_266 formosanin C 3.51 406.0 nM 1.14E-4 sphingolipid biosynthesis & PDR1 81 2.26E-4
11 SGTC_392 N-ethylmaleimide 3.49 8.3 µM 1.23E-4 RNA pol III & RNase P/MRP 25 2.41E-4
12 SGTC_1069 diphenylcyclopropenone 3.48 8.5 µM 1.29E-4 46 2.52E-4
13 SGTC_702 1488-0190 3.46 94.0 µM 1.39E-4 amide catabolism 26 2.70E-4
14 SGTC_2288 7946289 3.41 167.3 µM 1.72E-4 49 3.27E-4
15 SGTC_158 k038-0087 3.30 26.4 µM 2.65E-4 ubiquinone biosynthesis & proteasome 185 4.87E-4
16 SGTC_2887 9044784 3.29 58.4 µM 2.73E-4 fatty acid desaturase (OLE1) 66 5.00E-4
17 SGTC_1892 st058411 3.29 7.4 µM 2.79E-4 52 5.09E-4
18 SGTC_2941 9056145 3.26 11.2 µM 3.07E-4 33 5.56E-4
19 SGTC_1445 4239-0156 3.23 97.7 µM 3.41E-4 62 6.13E-4
20 SGTC_2336 9001819 3.12 198.7 µM 5.19E-4 heme biosynthesis & mitochondrial translocase 90 9.01E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.344 1.00E-93 YHL045W_d YHL045W_d hom Putative protein of unknown function; not an essential gene
0.341 8.11E-92 YPL246C RBD2 hom Possible rhomboid protease, has similarity to eukaryotic rhomboid proteases including Pcp1p
0.313 4.60E-77 YDL155W CLB3 hom B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the G2/M transition; may be involved in DNA replication and spindle assembly; accumulates during S phase and G2, then targeted for ubiquitin-mediated degradation; relative distribution to the nucleus increases upon DNA replication stress
0.285 8.58E-64 YIL009W FAA3 hom Long chain fatty acyl-CoA synthetase, activates imported fatty acids; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery
0.268 2.69E-56 YOR271C_p FSF1_p hom Putative protein, predicted to be an alpha-isopropylmalate carrier; belongs to the sideroblastic-associated protein family; non-tagged protein is detected in purified mitochondria; likely to play a role in iron homeostasis
0.256 1.94E-51 YML025C YML6 het Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L4 ribosomal protein and human mitoribosomal MRP-L4 protein; essential for viability, unlike most other mitoribosomal proteins
0.250 1.22E-48 YDR242W AMD2 hom Putative amidase
0.249 9.17E-49 YLR040C_p YLR040C_p hom Protein of unknown function; localizes to the cell wall; predicted to be a GPI-attached protein; upregulated by Mcm1p-Alpha1p transcription factor; partially overlaps the dubious ORF YLR041W; mRNA identified as translated by ribosome profiling data; YLR040C is not essential
0.245 3.10E-47 YNL123W NMA111 hom Serine protease and general molecular chaperone; involved in response to heat stress and promotion of apoptosis; may contribute to lipid homeostasis; sequence similarity to the mammalian Omi/HtrA2 family of serine proteases
0.244 1.21E-46 YGL090W LIF1 hom Component of the DNA ligase IV complex that mediates nonhomologous end joining in DNA double-strand break repair; physically interacts with Dnl4p and Nej1p; homologous to mammalian XRCC4 protein
0.233 1.88E-42 YLR317W_d YLR317W_d het Dubious open reading frame; may be part of a bicistronic transcript with NKP2/YLR315W; overlaps the verified ORF TAD3/YLR316C
0.233 1.96E-42 YGL114W_p YGL114W_p hom Putative protein of unknown function; predicted member of the oligopeptide transporter (OPT) family of membrane transporters
0.227 1.61E-40 YDL022W GPD1 hom NAD-dependent glycerol-3-phosphate dehydrogenase; key enzyme of glycerol synthesis, essential for growth under osmotic stress; expression regulated by high-osmolarity glycerol response pathway; homolog of Gpd2p; protein abundance increases in response to DNA replication stress; constitutively inactivated via phosphorylation by the protein kinases YPK1 and YPK2, dephosphorylation increases catalytic activity
0.217 6.90E-37 YCR015C_p YCR015C_p hom Putative protein of unknown function; YCR015C is not an essential gene
0.210 8.12E-35 YPL113C_p YPL113C_p hom Glyoxylate reductase; acts on glyoxylate and hydroxypyruvate substrates; YPL113C is not an essential gene