YML036W / CGI121

Component of the EKC/KEOPS complex with Bud32p, Kae1p, Pcc1p, and Gon7p; EKC/KEOPS complex is required for t6A tRNA modification and may have roles in telomere maintenance and transcription; Cgi121p is dispensable for tRNA modification

Zygosity: Homozygous strain
fixedexpanded
Profile for YML036W / CGI121

Click on Significant Values for Screen Details ID:SGTC_46|Compound:0329-0187a|FD-Score:3.94|P-value:1.37E-4 ID:SGTC_65|Compound:0798-0905|FD-Score:-3.52|P-value:5.13E-4 ID:SGTC_87|Compound:0100-0052|FD-Score:3.86|P-value:1.85E-4 ID:SGTC_195|Compound:4234-0266|FD-Score:5.97|P-value:1.71E-8 ID:SGTC_221|Compound:trifluoperazine|FD-Score:5.39|P-value:3.14E-7 ID:SGTC_232|Compound:clozapine|FD-Score:4.79|P-value:4.82E-6 ID:SGTC_250|Compound:brefeldin a|FD-Score:3.46|P-value:7.11E-4 ID:SGTC_253|Compound:dyclonine|FD-Score:3.43|P-value:7.88E-4 ID:SGTC_284|Compound:0988-0104|FD-Score:-3.89|P-value:1.44E-4 ID:SGTC_299|Compound:1273-0059|FD-Score:-5.16|P-value:7.77E-7 ID:SGTC_301|Compound:4119-0100|FD-Score:5.17|P-value:8.66E-7 ID:SGTC_310|Compound:1273-0058|FD-Score:-5.01|P-value:1.49E-6 ID:SGTC_364|Compound:0083-0097|FD-Score:3.36|P-value:9.64E-4 ID:SGTC_375|Compound:3970-0961|FD-Score:3.84|P-value:1.98E-4 ID:SGTC_422|Compound:cantharidin|FD-Score:6.53|P-value:7.70E-10 ID:SGTC_431|Compound:k089-0097|FD-Score:-4.16|P-value:5.23E-5 ID:SGTC_445|Compound:e6 berbamine|FD-Score:5.02|P-value:1.72E-6 ID:SGTC_465|Compound:cgp-37157|FD-Score:-3.72|P-value:2.56E-4 ID:SGTC_535|Compound:2293-4129|FD-Score:6.42|P-value:1.43E-9 ID:SGTC_568|Compound:1193-0097|FD-Score:-3.87|P-value:1.52E-4 ID:SGTC_656|Compound:4090-1979|FD-Score:3.64|P-value:3.85E-4 ID:SGTC_686|Compound:1319-0111|FD-Score:4.29|P-value:3.76E-5 ID:SGTC_830|Compound:4076-0286|FD-Score:3.88|P-value:1.71E-4 ID:SGTC_831|Compound:k061-1017|FD-Score:4.42|P-value:2.25E-5 ID:SGTC_832|Compound:4083-0019|FD-Score:3.66|P-value:3.65E-4 ID:SGTC_1127|Compound:asiatic acid|FD-Score:7.73|P-value:4.06E-13 ID:SGTC_1130|Compound:3454-2858|FD-Score:4.03|P-value:9.88E-5 ID:SGTC_1132|Compound:3456-3614|FD-Score:6.26|P-value:3.53E-9 ID:SGTC_1137|Compound:ixabepilone|FD-Score:-3.79|P-value:2.06E-4 ID:SGTC_1186|Compound:1319-0216|FD-Score:3.47|P-value:6.80E-4 ID:SGTC_1446|Compound:4239-0190|FD-Score:5.05|P-value:1.50E-6 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:-3.58|P-value:4.14E-4 ID:SGTC_1618|Compound:st003135|FD-Score:-3.71|P-value:2.65E-4 ID:SGTC_1658|Compound:st012944|FD-Score:-3.42|P-value:7.05E-4 ID:SGTC_1718|Compound:roemerine|FD-Score:-5.43|P-value:2.07E-7 ID:SGTC_1754|Compound:st042065|FD-Score:4.03|P-value:9.90E-5 ID:SGTC_1781|Compound:st038725|FD-Score:6.90|P-value:8.57E-11 ID:SGTC_1797|Compound:st048695|FD-Score:4.14|P-value:6.48E-5 ID:SGTC_1800|Compound:st050079|FD-Score:4.16|P-value:6.17E-5 ID:SGTC_1803|Compound:st050068|FD-Score:4.18|P-value:5.72E-5 ID:SGTC_1930|Compound:st074634|FD-Score:-3.59|P-value:4.05E-4 ID:SGTC_1934|Compound:st074720|FD-Score:5.15|P-value:9.48E-7 ID:SGTC_2031|Compound:5180455|FD-Score:3.41|P-value:8.23E-4 ID:SGTC_2337|Compound:9009472|FD-Score:-3.87|P-value:1.54E-4 ID:SGTC_2606|Compound:farnesol|FD-Score:3.50|P-value:6.19E-4 ID:SGTC_2658|Compound:polymyxin b sulfate|FD-Score:3.79|P-value:2.32E-4 ID:SGTC_2759|Compound:disulfiram|FD-Score:-3.84|P-value:1.70E-4 ID:SGTC_2831|Compound:7999036|FD-Score:-3.65|P-value:3.25E-4 ID:SGTC_2832|Compound:7992762|FD-Score:3.36|P-value:9.56E-4 ID:SGTC_2943|Compound:9068529|FD-Score:3.36|P-value:9.70E-4 ID:SGTC_2952|Compound:9073731|FD-Score:5.38|P-value:3.27E-7 ID:SGTC_2974|Compound:9094440|FD-Score:-5.08|P-value:1.12E-6 ID:SGTC_3011|Compound:9081274|FD-Score:3.60|P-value:4.44E-4 ID:SGTC_3015|Compound:9080845|FD-Score:3.73|P-value:2.91E-4 ID:SGTC_3078|Compound:9116413|FD-Score:4.35|P-value:2.96E-5 ID:SGTC_3219|Compound:9131112|FD-Score:-3.82|P-value:1.82E-4 ID:SGTC_3230|Compound:9131665|FD-Score:-3.35|P-value:8.80E-4 ID:SGTC_3232|Compound:9132874|FD-Score:-4.01|P-value:9.19E-5 ID:SGTC_3238|Compound:9133470|FD-Score:-3.40|P-value:7.43E-4 ID:SGTC_46|Compound:0329-0187a|FD-Score:3.94|P-value:1.37E-4 ID:SGTC_65|Compound:0798-0905|FD-Score:-3.52|P-value:5.13E-4 ID:SGTC_87|Compound:0100-0052|FD-Score:3.86|P-value:1.85E-4 ID:SGTC_195|Compound:4234-0266|FD-Score:5.97|P-value:1.71E-8 ID:SGTC_221|Compound:trifluoperazine|FD-Score:5.39|P-value:3.14E-7 ID:SGTC_232|Compound:clozapine|FD-Score:4.79|P-value:4.82E-6 ID:SGTC_250|Compound:brefeldin a|FD-Score:3.46|P-value:7.11E-4 ID:SGTC_253|Compound:dyclonine|FD-Score:3.43|P-value:7.88E-4 ID:SGTC_284|Compound:0988-0104|FD-Score:-3.89|P-value:1.44E-4 ID:SGTC_299|Compound:1273-0059|FD-Score:-5.16|P-value:7.77E-7 ID:SGTC_301|Compound:4119-0100|FD-Score:5.17|P-value:8.66E-7 ID:SGTC_310|Compound:1273-0058|FD-Score:-5.01|P-value:1.49E-6 ID:SGTC_364|Compound:0083-0097|FD-Score:3.36|P-value:9.64E-4 ID:SGTC_375|Compound:3970-0961|FD-Score:3.84|P-value:1.98E-4 ID:SGTC_422|Compound:cantharidin|FD-Score:6.53|P-value:7.70E-10 ID:SGTC_431|Compound:k089-0097|FD-Score:-4.16|P-value:5.23E-5 ID:SGTC_445|Compound:e6 berbamine|FD-Score:5.02|P-value:1.72E-6 ID:SGTC_465|Compound:cgp-37157|FD-Score:-3.72|P-value:2.56E-4 ID:SGTC_535|Compound:2293-4129|FD-Score:6.42|P-value:1.43E-9 ID:SGTC_568|Compound:1193-0097|FD-Score:-3.87|P-value:1.52E-4 ID:SGTC_656|Compound:4090-1979|FD-Score:3.64|P-value:3.85E-4 ID:SGTC_686|Compound:1319-0111|FD-Score:4.29|P-value:3.76E-5 ID:SGTC_830|Compound:4076-0286|FD-Score:3.88|P-value:1.71E-4 ID:SGTC_831|Compound:k061-1017|FD-Score:4.42|P-value:2.25E-5 ID:SGTC_832|Compound:4083-0019|FD-Score:3.66|P-value:3.65E-4 ID:SGTC_1127|Compound:asiatic acid|FD-Score:7.73|P-value:4.06E-13 ID:SGTC_1130|Compound:3454-2858|FD-Score:4.03|P-value:9.88E-5 ID:SGTC_1132|Compound:3456-3614|FD-Score:6.26|P-value:3.53E-9 ID:SGTC_1137|Compound:ixabepilone|FD-Score:-3.79|P-value:2.06E-4 ID:SGTC_1186|Compound:1319-0216|FD-Score:3.47|P-value:6.80E-4 ID:SGTC_1446|Compound:4239-0190|FD-Score:5.05|P-value:1.50E-6 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:-3.58|P-value:4.14E-4 ID:SGTC_1618|Compound:st003135|FD-Score:-3.71|P-value:2.65E-4 ID:SGTC_1658|Compound:st012944|FD-Score:-3.42|P-value:7.05E-4 ID:SGTC_1718|Compound:roemerine|FD-Score:-5.43|P-value:2.07E-7 ID:SGTC_1754|Compound:st042065|FD-Score:4.03|P-value:9.90E-5 ID:SGTC_1781|Compound:st038725|FD-Score:6.90|P-value:8.57E-11 ID:SGTC_1797|Compound:st048695|FD-Score:4.14|P-value:6.48E-5 ID:SGTC_1800|Compound:st050079|FD-Score:4.16|P-value:6.17E-5 ID:SGTC_1803|Compound:st050068|FD-Score:4.18|P-value:5.72E-5 ID:SGTC_1930|Compound:st074634|FD-Score:-3.59|P-value:4.05E-4 ID:SGTC_1934|Compound:st074720|FD-Score:5.15|P-value:9.48E-7 ID:SGTC_2031|Compound:5180455|FD-Score:3.41|P-value:8.23E-4 ID:SGTC_2337|Compound:9009472|FD-Score:-3.87|P-value:1.54E-4 ID:SGTC_2606|Compound:farnesol|FD-Score:3.50|P-value:6.19E-4 ID:SGTC_2658|Compound:polymyxin b sulfate|FD-Score:3.79|P-value:2.32E-4 ID:SGTC_2759|Compound:disulfiram|FD-Score:-3.84|P-value:1.70E-4 ID:SGTC_2831|Compound:7999036|FD-Score:-3.65|P-value:3.25E-4 ID:SGTC_2832|Compound:7992762|FD-Score:3.36|P-value:9.56E-4 ID:SGTC_2943|Compound:9068529|FD-Score:3.36|P-value:9.70E-4 ID:SGTC_2952|Compound:9073731|FD-Score:5.38|P-value:3.27E-7 ID:SGTC_2974|Compound:9094440|FD-Score:-5.08|P-value:1.12E-6 ID:SGTC_3011|Compound:9081274|FD-Score:3.60|P-value:4.44E-4 ID:SGTC_3015|Compound:9080845|FD-Score:3.73|P-value:2.91E-4 ID:SGTC_3078|Compound:9116413|FD-Score:4.35|P-value:2.96E-5 ID:SGTC_3219|Compound:9131112|FD-Score:-3.82|P-value:1.82E-4 ID:SGTC_3230|Compound:9131665|FD-Score:-3.35|P-value:8.80E-4 ID:SGTC_3232|Compound:9132874|FD-Score:-4.01|P-value:9.19E-5 ID:SGTC_3238|Compound:9133470|FD-Score:-3.40|P-value:7.43E-4

Top fitness defect scores for YML036W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1127 asiatic acid 7.73 681.4 nM 4.06E-13 1 5.21E-15
2 SGTC_1781 st038725 6.90 66.3 µM 8.57E-11 NEO1 41 2.60E-12
3 SGTC_422 cantharidin 6.53 155.0 µM 7.70E-10 cell wall signaling 16 3.32E-11
4 SGTC_535 2293-4129 6.42 48.2 µM 1.43E-9 NEO1 24 6.83E-11
5 SGTC_1132 3456-3614 6.26 2.5 µM 3.53E-9 tubulin folding & SWR complex 10 1.94E-10
6 SGTC_195 4234-0266 5.97 16.6 µM 1.71E-8 7 1.21E-9
7 SGTC_221 trifluoperazine 5.39 10.3 µM 3.14E-7 NEO1-PIK1 148 3.54E-8
8 SGTC_2952 9073731 5.38 13.8 µM 3.27E-7 tubulin folding & SWR complex 10 3.71E-8
9 SGTC_301 4119-0100 5.17 24.5 µM 8.66E-7 NEO1 49 1.15E-7
10 SGTC_1934 st074720 5.15 32.6 µM 9.48E-7 24 1.28E-7
11 SGTC_1446 4239-0190 5.05 62.0 µM 1.50E-6 17 2.18E-7
12 SGTC_445 e6 berbamine 5.02 66.0 µM 1.72E-6 NEO1-PIK1 62 2.55E-7
13 SGTC_232 clozapine 4.79 119.0 µM 4.82E-6 NEO1 50 8.40E-7
14 SGTC_831 k061-1017 4.42 217.0 µM 2.25E-5 translation 40 5.02E-6
15 SGTC_3078 9116413 4.35 49.5 µM 2.96E-5 unfolded protein response 28 6.89E-6
16 SGTC_686 1319-0111 4.29 510.0 µM 3.76E-5 60S ribosome export 23 9.07E-6
17 SGTC_1803 st050068 4.18 52.4 µM 5.72E-5 NEO1-PIK1 165 1.48E-5
18 SGTC_1800 st050079 4.16 29.1 µM 6.17E-5 NEO1-PIK1 110 1.61E-5
19 SGTC_1797 st048695 4.14 56.8 µM 6.48E-5 NEO1 67 1.70E-5
20 SGTC_1130 3454-2858 4.03 15.9 µM 9.88E-5 NEO1 87 2.78E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.315 3.70E-78 YIL048W NEO1 het Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus
0.293 3.36E-67 YJL053W PEP8 hom Vacuolar protein component of the retromer; forms part of the multimeric membrane-associated retromer complex involved in vacuolar protein sorting along with Vps35p, Vps29p, Vps17p, and Vps5p; essential for endosome-to-Golgi retrograde protein transport; interacts with Ypt7p; protein abundance increases in response to DNA replication stress
0.286 2.21E-64 YJL154C VPS35 hom Endosomal subunit of membrane-associated retromer complex required for retrograde transport; receptor that recognizes retrieval signals on cargo proteins, forms subcomplex with Vps26p and Vps29p that selects cargo proteins for retrieval; interacts with Ypt7p
0.284 5.34E-63 YOR293W RPS10A hom Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S10, no bacterial homolog; RPS10A has a paralog, RPS10B, that arose from the whole genome duplication
0.282 3.20E-62 YDR378C LSM6 hom Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA
0.273 4.22E-58 YOR039W CKB2 hom Beta' regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerase
0.272 4.12E-58 YHR012W VPS29 hom Endosomal protein that is a subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde transport; forms a subcomplex with Vps35p and Vps26p that selects cargo proteins for endosome-to-Golgi retrieval
0.250 8.30E-49 YGL019W CKB1 hom Beta regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases
0.244 8.06E-47 YDR455C_d YDR455C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YDR456W
0.236 8.40E-44 YJL052W TDH1 hom Glyceraldehyde-3-phosphate dehydrogenase, isozyme 1; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; protein abundance increases in response to DNA replication stress
0.230 2.53E-41 YDR372C VPS74 hom Golgi phosphatidylinositol-4-kinase effector and PtdIns4P sensor; interacts with the cytosolic domains of cis and medial glycosyltransferases, and in the PtdIns4P-bound state mediates the targeting of these enzymes to the Golgi; interacts with the catalytic domain of Sac1p, the major cellular PtdIns4P phosphatase, to direct dephosphosphorylation of the Golgi pool of PtdIns4P; tetramerization required for function; ortholog of human GOLPH3/GPP34/GMx33
0.226 6.99E-40 YFL023W BUD27 hom Unconventional prefoldin protein involved in translation initiation; mutants have inappropriate expression of nutrient sensitive genes due to translational derepression of Gcn4p transcription factor; diploid mutants show random budding; ortholog of human URI/RMP
0.225 1.02E-39 YDL033C SLM3 hom tRNA-specific 2-thiouridylase, responsible for 2-thiolation of the wobble base of mitochondrial tRNAs; human ortholog is implicated in myoclonus epilepsy associated with ragged red fibers (MERRF)
0.224 3.17E-39 YJL124C LSM1 hom Lsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) involved in degradation of cytoplasmic mRNAs; unlike most Sm-like proteins, Lsm1p requires both its SM-domain and C-terminal domain for RNA-binding; forms cytoplasmic foci upon DNA replication stress
0.222 8.33E-39 YCR064C_d YCR064C_d hom Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene BUD31