YML058W-A / HUG1

Protein involved in the Mec1p-mediated checkpoint pathway; transcription is induced by DNA damage; protein abundance increases in response to DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YML058W-A / HUG1

Click on Significant Values for Screen Details ID:SGTC_20|Compound:0481-0013|FD-Score:3.32|P-value:2.65E-4 ID:SGTC_49|Compound:0717-0965|FD-Score:-3.87|P-value:2.67E-5 ID:SGTC_50|Compound:0929-0078|FD-Score:-3.21|P-value:3.95E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:3.19|P-value:4.32E-4 ID:SGTC_97|Compound:0535-0701|FD-Score:3.39|P-value:1.98E-4 ID:SGTC_175|Compound:3793-3120|FD-Score:4.14|P-value:7.54E-6 ID:SGTC_179|Compound:1273-0063|FD-Score:3.04|P-value:7.40E-4 ID:SGTC_236|Compound:1509-0011|FD-Score:3.13|P-value:5.43E-4 ID:SGTC_289|Compound:k017-0060|FD-Score:3.26|P-value:3.35E-4 ID:SGTC_424|Compound:splitomicin|FD-Score:-3.21|P-value:3.98E-4 ID:SGTC_574|Compound:1315-0397|FD-Score:3.20|P-value:4.14E-4 ID:SGTC_596|Compound:1155-0152|FD-Score:4.08|P-value:1.04E-5 ID:SGTC_756|Compound:0508-1999|FD-Score:3.25|P-value:3.51E-4 ID:SGTC_834|Compound:0312-0008|FD-Score:4.28|P-value:4.01E-6 ID:SGTC_941|Compound:3237-0776|FD-Score:3.87|P-value:2.69E-5 ID:SGTC_1205|Compound:4161-2785|FD-Score:4.02|P-value:1.34E-5 ID:SGTC_1223|Compound:0764-0573|FD-Score:4.09|P-value:9.69E-6 ID:SGTC_1224|Compound:0823-0416|FD-Score:3.13|P-value:5.47E-4 ID:SGTC_1238|Compound:0333-0287|FD-Score:3.12|P-value:5.63E-4 ID:SGTC_1298|Compound:1079-1278|FD-Score:-3.20|P-value:4.17E-4 ID:SGTC_1310|Compound:1188-0297|FD-Score:-2.98|P-value:9.34E-4 ID:SGTC_1357|Compound:1503-1303|FD-Score:3.24|P-value:3.57E-4 ID:SGTC_1580|Compound:hydrocortisone 21-hemisuccinate|FD-Score:3.27|P-value:3.18E-4 ID:SGTC_1646|Compound:st011709|FD-Score:-3.05|P-value:7.11E-4 ID:SGTC_1703|Compound:st026983|FD-Score:3.30|P-value:2.83E-4 ID:SGTC_1768|Compound:st044301|FD-Score:3.61|P-value:8.20E-5 ID:SGTC_1773|Compound:st048542|FD-Score:3.59|P-value:9.03E-5 ID:SGTC_1939|Compound:st074455|FD-Score:-4.02|P-value:1.33E-5 ID:SGTC_1977|Compound:st070720|FD-Score:3.02|P-value:8.14E-4 ID:SGTC_1985|Compound:st071382|FD-Score:4.66|P-value:5.72E-7 ID:SGTC_2074|Compound:5212007|FD-Score:3.21|P-value:4.03E-4 ID:SGTC_2175|Compound:5807670|FD-Score:3.46|P-value:1.51E-4 ID:SGTC_2185|Compound:6113692|FD-Score:3.87|P-value:2.62E-5 ID:SGTC_2358|Compound:9037157|FD-Score:3.81|P-value:3.47E-5 ID:SGTC_2364|Compound:9055074|FD-Score:-3.86|P-value:2.79E-5 ID:SGTC_2804|Compound:7954240|FD-Score:3.52|P-value:1.20E-4 ID:SGTC_2894|Compound:9048625|FD-Score:3.01|P-value:8.41E-4 ID:SGTC_2925|Compound:9010536|FD-Score:-3.06|P-value:6.87E-4 ID:SGTC_3023|Compound:9089636|FD-Score:-3.10|P-value:5.90E-4 ID:SGTC_3027|Compound:9089541|FD-Score:3.06|P-value:7.09E-4 ID:SGTC_3031|Compound:9088762|FD-Score:3.63|P-value:7.37E-5 ID:SGTC_3234|Compound:9132280|FD-Score:4.21|P-value:5.59E-6 ID:SGTC_3310|Compound:9130229|FD-Score:2.97|P-value:9.74E-4 ID:SGTC_20|Compound:0481-0013|FD-Score:3.32|P-value:2.65E-4 ID:SGTC_49|Compound:0717-0965|FD-Score:-3.87|P-value:2.67E-5 ID:SGTC_50|Compound:0929-0078|FD-Score:-3.21|P-value:3.95E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:3.19|P-value:4.32E-4 ID:SGTC_97|Compound:0535-0701|FD-Score:3.39|P-value:1.98E-4 ID:SGTC_175|Compound:3793-3120|FD-Score:4.14|P-value:7.54E-6 ID:SGTC_179|Compound:1273-0063|FD-Score:3.04|P-value:7.40E-4 ID:SGTC_236|Compound:1509-0011|FD-Score:3.13|P-value:5.43E-4 ID:SGTC_289|Compound:k017-0060|FD-Score:3.26|P-value:3.35E-4 ID:SGTC_424|Compound:splitomicin|FD-Score:-3.21|P-value:3.98E-4 ID:SGTC_574|Compound:1315-0397|FD-Score:3.20|P-value:4.14E-4 ID:SGTC_596|Compound:1155-0152|FD-Score:4.08|P-value:1.04E-5 ID:SGTC_756|Compound:0508-1999|FD-Score:3.25|P-value:3.51E-4 ID:SGTC_834|Compound:0312-0008|FD-Score:4.28|P-value:4.01E-6 ID:SGTC_941|Compound:3237-0776|FD-Score:3.87|P-value:2.69E-5 ID:SGTC_1205|Compound:4161-2785|FD-Score:4.02|P-value:1.34E-5 ID:SGTC_1223|Compound:0764-0573|FD-Score:4.09|P-value:9.69E-6 ID:SGTC_1224|Compound:0823-0416|FD-Score:3.13|P-value:5.47E-4 ID:SGTC_1238|Compound:0333-0287|FD-Score:3.12|P-value:5.63E-4 ID:SGTC_1298|Compound:1079-1278|FD-Score:-3.20|P-value:4.17E-4 ID:SGTC_1310|Compound:1188-0297|FD-Score:-2.98|P-value:9.34E-4 ID:SGTC_1357|Compound:1503-1303|FD-Score:3.24|P-value:3.57E-4 ID:SGTC_1580|Compound:hydrocortisone 21-hemisuccinate|FD-Score:3.27|P-value:3.18E-4 ID:SGTC_1646|Compound:st011709|FD-Score:-3.05|P-value:7.11E-4 ID:SGTC_1703|Compound:st026983|FD-Score:3.30|P-value:2.83E-4 ID:SGTC_1768|Compound:st044301|FD-Score:3.61|P-value:8.20E-5 ID:SGTC_1773|Compound:st048542|FD-Score:3.59|P-value:9.03E-5 ID:SGTC_1939|Compound:st074455|FD-Score:-4.02|P-value:1.33E-5 ID:SGTC_1977|Compound:st070720|FD-Score:3.02|P-value:8.14E-4 ID:SGTC_1985|Compound:st071382|FD-Score:4.66|P-value:5.72E-7 ID:SGTC_2074|Compound:5212007|FD-Score:3.21|P-value:4.03E-4 ID:SGTC_2175|Compound:5807670|FD-Score:3.46|P-value:1.51E-4 ID:SGTC_2185|Compound:6113692|FD-Score:3.87|P-value:2.62E-5 ID:SGTC_2358|Compound:9037157|FD-Score:3.81|P-value:3.47E-5 ID:SGTC_2364|Compound:9055074|FD-Score:-3.86|P-value:2.79E-5 ID:SGTC_2804|Compound:7954240|FD-Score:3.52|P-value:1.20E-4 ID:SGTC_2894|Compound:9048625|FD-Score:3.01|P-value:8.41E-4 ID:SGTC_2925|Compound:9010536|FD-Score:-3.06|P-value:6.87E-4 ID:SGTC_3023|Compound:9089636|FD-Score:-3.10|P-value:5.90E-4 ID:SGTC_3027|Compound:9089541|FD-Score:3.06|P-value:7.09E-4 ID:SGTC_3031|Compound:9088762|FD-Score:3.63|P-value:7.37E-5 ID:SGTC_3234|Compound:9132280|FD-Score:4.21|P-value:5.59E-6 ID:SGTC_3310|Compound:9130229|FD-Score:2.97|P-value:9.74E-4

Top fitness defect scores for YML058W-A deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1985 st071382 4.66 65.5 µM 5.72E-7 10 1.59E-6
2 SGTC_834 0312-0008 4.28 75.7 µM 4.01E-6 NEO1 26 9.53E-6
3 SGTC_3234 9132280 4.21 49.5 µM 5.59E-6 13 1.29E-5
4 SGTC_175 3793-3120 4.14 23.7 µM 7.54E-6 10 1.70E-5
5 SGTC_1223 0764-0573 4.09 147.0 µM 9.69E-6 18 2.15E-5
6 SGTC_596 1155-0152 4.08 116.0 µM 1.04E-5 TRP & mitochondrial translation 33 2.30E-5
7 SGTC_1205 4161-2785 4.02 76.0 µM 1.34E-5 28 2.89E-5
8 SGTC_2185 6113692 3.87 200.0 µM 2.62E-5 ergosterol biosynthesis 19 5.36E-5
9 SGTC_941 3237-0776 3.87 460.0 µM 2.69E-5 16 5.49E-5
10 SGTC_2358 9037157 3.81 4.0 µM 3.47E-5 37 6.94E-5
11 SGTC_3031 9088762 3.63 49.5 µM 7.37E-5 30 1.39E-4
12 SGTC_1768 st044301 3.61 37.2 µM 8.20E-5 24 1.53E-4
13 SGTC_1773 st048542 3.59 73.6 µM 9.03E-5 58 1.68E-4
14 SGTC_2804 7954240 3.52 19.5 µM 1.20E-4 49 2.17E-4
15 SGTC_2175 5807670 3.46 165.3 µM 1.51E-4 31 2.69E-4
16 SGTC_97 0535-0701 3.39 19.1 µM 1.98E-4 60S ribosome export 33 3.46E-4
17 SGTC_20 0481-0013 3.32 4.9 µM 2.65E-4 48 4.53E-4
18 SGTC_1703 st026983 3.30 48.3 µM 2.83E-4 28 4.81E-4
19 SGTC_1580 hydrocortisone 21-hemisuccinate 3.27 43.2 µM 3.18E-4 43 5.35E-4
20 SGTC_289 k017-0060 3.26 526.0 µM 3.35E-4 10 5.61E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.123 8.64E-13 YDR032C PST2 hom Protein with similarity to a family of flavodoxin-like proteins; induced by oxidative stress in a Yap1p dependent manner; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress; PST2 has a paralog, RFS1, that arose from the whole genome duplication
0.111 1.20E-10 YGL107C RMD9 hom Mitochondrial protein required for respiratory growth; mutant phenotype and genetic interactions suggest a role in delivering mt mRNAs to ribosomes; located on matrix face of the inner membrane and loosely associated with mitoribosomes
0.110 1.66E-10 YGL237C HAP2 hom Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; contains sequences sufficient for both complex assembly and DNA binding
0.110 1.87E-10 YHR050W-A_p YHR050W-A_p hom Protein of unknown function; identified by expression profiling and mass spectrometry
0.107 4.73E-10 YMR286W MRPL33 hom Mitochondrial ribosomal protein of the large subunit
0.105 1.21E-9 YJR120W YJR120W hom Protein of unknown function; essential for growth under anaerobic conditions; mutation causes decreased expression of ATP2, impaired respiration, defective sterol uptake, and altered levels/localization of ABC transporters Aus1p and Pdr11p
0.103 2.18E-9 YOR330C MIP1 hom Mitochondrial DNA polymerase; conserved C-terminal segment is required for the maintenance of mitochondrial genome; mutations in the human ortholog POLG are associated with Alpers-Huttenlocher syndrome (AHS) and other mitochondrial diseases; Mip1p is the single subunit of mitochondrial DNA polymerase in yeast, in contrast to metazoans in which there is a complex of a catalytic subunit and an accessory subunit
0.102 2.93E-9 YJL166W QCR8 hom Subunit 8 of ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the intermembrane space; expression is regulated by Abf1p and Cpf1p
0.100 6.43E-9 YDR205W MSC2 hom Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Zrg17p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; localizes to ER and nucleus; mutations affect the cellular distribution of zinc and also confer defects in meiotic recombination between homologous chromatids
0.100 6.76E-9 YCR060W TAH1 hom Component of the conserved R2TP complex (Rvb1-Rvb2-Tah1-Pih1); contains a single TPR domain with at least two TPR motifs; R2TP complex interacts with Hsp90 (Hsp82p and Hsc82p) to mediate assembly large protein complexes such as box C/D snoRNPs and RNA polymerase II
0.098 1.18E-8 YJL023C PET130 hom Protein required for respiratory growth; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.098 1.21E-8 YHL038C CBP2 hom Required for splicing of the group I intron bI5 of the COB pre-mRNA; nuclear-encoded mitochondrial protein that binds to the RNA to promote splicing; also involved in but not essential for splicing of the COB bI2 intron and the intron in the 21S rRNA gene
0.098 1.35E-8 YMR035W IMP2 hom Catalytic subunit of the mitochondrial inner membrane peptidase complex, required for maturation of mitochondrial proteins of the intermembrane space; complex contains Imp1p and Imp2p (both catalytic subunits), and Som1p
0.098 1.49E-8 YNL184C_p YNL184C_p hom Protein of unknown function; expressed at both mRNA and protein levels
0.097 1.91E-8 YBR156C SLI15 hom Subunit of the conserved chromosomal passenger complex (CPC; Ipl1p-Sli15p-Bir1p-Nbl1p), which regulates kinetochore-microtubule attachments, activation of the spindle tension checkpoint, and mitotic spindle disassembly