YML065W / ORC1

Largest subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; exhibits ATPase activity

Zygosity: Heterozygous strain
fixedexpanded
Profile for YML065W / ORC1

Click on Significant Values for Screen Details ID:SGTC_47|Compound:0149-0135|FD-Score:-4.15|P-value:2.33E-7 ID:SGTC_57|Compound:miconazole|FD-Score:2.89|P-value:2.23E-4 ID:SGTC_151|Compound:3013-0144|FD-Score:2.66|P-value:6.17E-4 ID:SGTC_162|Compound:k030-0015|FD-Score:-2.65|P-value:6.49E-4 ID:SGTC_282|Compound:k297-0036|FD-Score:2.76|P-value:4.02E-4 ID:SGTC_372|Compound:0155-0182|FD-Score:-3.42|P-value:1.65E-5 ID:SGTC_595|Compound:3909-8003|FD-Score:3.34|P-value:2.52E-5 ID:SGTC_613|Compound:3914-0030|FD-Score:-3.63|P-value:5.20E-6 ID:SGTC_615|Compound:k087-0205|FD-Score:-3.15|P-value:6.29E-5 ID:SGTC_616|Compound:4092-0450|FD-Score:-4.56|P-value:1.45E-8 ID:SGTC_787|Compound:4152-0075|FD-Score:-2.58|P-value:8.37E-4 ID:SGTC_907|Compound:3448-6986|FD-Score:-2.98|P-value:1.45E-4 ID:SGTC_910|Compound:3454-3003|FD-Score:2.67|P-value:5.86E-4 ID:SGTC_916|Compound:4466-0038|FD-Score:3.02|P-value:1.22E-4 ID:SGTC_1129|Compound:3453-1384|FD-Score:-2.90|P-value:2.09E-4 ID:SGTC_1132|Compound:3456-3614|FD-Score:2.76|P-value:3.97E-4 ID:SGTC_1140|Compound:3229-1848|FD-Score:2.56|P-value:9.40E-4 ID:SGTC_1680|Compound:diphenhydramine|FD-Score:-3.15|P-value:6.51E-5 ID:SGTC_1705|Compound:st027868|FD-Score:2.75|P-value:4.23E-4 ID:SGTC_1740|Compound:st038286|FD-Score:-2.59|P-value:8.34E-4 ID:SGTC_1924|Compound:st058459|FD-Score:2.71|P-value:5.13E-4 ID:SGTC_2203|Compound:7227154|FD-Score:-2.68|P-value:5.55E-4 ID:SGTC_2312|Compound:7706453|FD-Score:3.61|P-value:5.93E-6 ID:SGTC_2595|Compound:hexamethylquercetagetin|FD-Score:3.09|P-value:8.74E-5 ID:SGTC_2602|Compound:kawain|FD-Score:2.55|P-value:9.69E-4 ID:SGTC_2696|Compound:st077691|FD-Score:2.79|P-value:3.52E-4 ID:SGTC_2794|Compound:7785382|FD-Score:2.82|P-value:3.14E-4 ID:SGTC_3196|Compound:9111891|FD-Score:-3.18|P-value:5.55E-5 ID:SGTC_47|Compound:0149-0135|FD-Score:-4.15|P-value:2.33E-7 ID:SGTC_57|Compound:miconazole|FD-Score:2.89|P-value:2.23E-4 ID:SGTC_151|Compound:3013-0144|FD-Score:2.66|P-value:6.17E-4 ID:SGTC_162|Compound:k030-0015|FD-Score:-2.65|P-value:6.49E-4 ID:SGTC_282|Compound:k297-0036|FD-Score:2.76|P-value:4.02E-4 ID:SGTC_372|Compound:0155-0182|FD-Score:-3.42|P-value:1.65E-5 ID:SGTC_595|Compound:3909-8003|FD-Score:3.34|P-value:2.52E-5 ID:SGTC_613|Compound:3914-0030|FD-Score:-3.63|P-value:5.20E-6 ID:SGTC_615|Compound:k087-0205|FD-Score:-3.15|P-value:6.29E-5 ID:SGTC_616|Compound:4092-0450|FD-Score:-4.56|P-value:1.45E-8 ID:SGTC_787|Compound:4152-0075|FD-Score:-2.58|P-value:8.37E-4 ID:SGTC_907|Compound:3448-6986|FD-Score:-2.98|P-value:1.45E-4 ID:SGTC_910|Compound:3454-3003|FD-Score:2.67|P-value:5.86E-4 ID:SGTC_916|Compound:4466-0038|FD-Score:3.02|P-value:1.22E-4 ID:SGTC_1129|Compound:3453-1384|FD-Score:-2.90|P-value:2.09E-4 ID:SGTC_1132|Compound:3456-3614|FD-Score:2.76|P-value:3.97E-4 ID:SGTC_1140|Compound:3229-1848|FD-Score:2.56|P-value:9.40E-4 ID:SGTC_1680|Compound:diphenhydramine|FD-Score:-3.15|P-value:6.51E-5 ID:SGTC_1705|Compound:st027868|FD-Score:2.75|P-value:4.23E-4 ID:SGTC_1740|Compound:st038286|FD-Score:-2.59|P-value:8.34E-4 ID:SGTC_1924|Compound:st058459|FD-Score:2.71|P-value:5.13E-4 ID:SGTC_2203|Compound:7227154|FD-Score:-2.68|P-value:5.55E-4 ID:SGTC_2312|Compound:7706453|FD-Score:3.61|P-value:5.93E-6 ID:SGTC_2595|Compound:hexamethylquercetagetin|FD-Score:3.09|P-value:8.74E-5 ID:SGTC_2602|Compound:kawain|FD-Score:2.55|P-value:9.69E-4 ID:SGTC_2696|Compound:st077691|FD-Score:2.79|P-value:3.52E-4 ID:SGTC_2794|Compound:7785382|FD-Score:2.82|P-value:3.14E-4 ID:SGTC_3196|Compound:9111891|FD-Score:-3.18|P-value:5.55E-5

Top fitness defect scores for YML065W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2312 7706453 3.61 155.7 µM 5.93E-6 17 1.54E-4 0.08
2 SGTC_595 3909-8003 3.34 73.7 µM 2.52E-5 7 4.20E-4 0.08
3 SGTC_2595 hexamethylquercetagetin 3.09 100.0 µM 8.74E-5 18 9.96E-4 0.02
4 SGTC_916 4466-0038 3.02 42.0 nM 1.22E-4 cell wall 43 0.00126 0.00
5 SGTC_57 miconazole 2.89 162.5 nM 2.23E-4 ergosterol depletion effects on membrane 44 0.00191 0.00
6 SGTC_2794 7785382 2.82 27.3 µM 3.14E-4 36 0.00243 0.08
7 SGTC_2696 st077691 2.79 74.3 µM 3.52E-4 31 0.00263 0.05
8 SGTC_1132 3456-3614 2.76 2.5 µM 3.97E-4 tubulin folding & SWR complex 20 0.00285 0.03
9 SGTC_282 k297-0036 2.76 65.7 µM 4.02E-4 17 0.00288 0.11
10 SGTC_1705 st027868 2.75 56.3 µM 4.23E-4 13 0.00299 0.08
11 SGTC_1924 st058459 2.71 75.1 µM 5.13E-4 13 0.00341 0.04
12 SGTC_910 3454-3003 2.67 490.0 µM 5.86E-4 21 0.00375 0.03
13 SGTC_151 3013-0144 2.66 2.3 µM 6.17E-4 iron homeostasis 33 0.00389 0.02
14 SGTC_1140 3229-1848 2.56 3.0 µM 9.40E-4 17 0.00521 0.02
15 SGTC_2602 kawain 2.55 100.0 µM 9.69E-4 19 0.00533 0.00
16 SGTC_1742 st038235 2.48 28.9 µM 0.00132 mitochondrial stress 36 0.00662 0.00
17 SGTC_608 k048-0125 2.45 159.0 µM 0.00145 28 0.00708 0.04
18 SGTC_1483 3453-0561 2.43 55.9 µM 0.00161 30 0.00761 0.00
19 SGTC_3202 9113761 2.40 49.5 µM 0.00181 42 0.00824 0.06
20 SGTC_1824 st053723 2.36 68.6 µM 0.00206 25 0.00903 0.04

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.151 1.17E-18 YDL224C WHI4 hom Putative RNA binding protein; regulates the cell size requirement for passage through Start and commitment to cell division; WHI4 has a paralog, WHI3, that arose from the whole genome duplication
0.146 2.23E-17 YOR093C_p YOR093C_p hom Putative protein of unknown function; deletion causes sensitivity to unfolded protein response-inducing agents
0.141 2.01E-16 YLL011W SOF1 het Essential protein required for biogenesis of 40S (small) ribosomal subunit; has similarity to the beta subunit of trimeric G-proteins and the splicing factor Prp4p
0.141 2.59E-16 YML046W PRP39 het U1 snRNP protein involved in splicing, contains multiple tetriatricopeptide repeats
0.138 8.20E-16 YDR504C SPG3 hom Protein required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources
0.136 2.20E-15 YOR195W SLK19 hom Kinetochore-associated protein required for normal segregation of chromosomes in meiosis and mitosis; component of the FEAR regulatory network, which promotes Cdc14p release from the nucleolus during anaphase; potential Cdc28p substrate
0.135 4.05E-15 YGR245C SDA1 het Highly conserved nuclear protein required for actin cytoskeleton organization and passage through Start, plays a critical role in G1 events, binds Nap1p, also involved in 60S ribosome biogenesis
0.128 1.22E-13 YGR003W CUL3 hom Ubiquitin-protein ligase, forms a complex with Elc1p that polyubiquitylates monoubiquitylated RNA polymerase II to trigger its proteolysis; cullin family member with similarity to Cdc53p and human CUL3
0.119 4.52E-12 YER104W RTT105 het Protein with a role in regulation of Ty1 transposition
0.111 1.09E-10 YMR140W SIP5 hom Protein of unknown function; interacts with both the Reg1p/Glc7p phosphatase and the Snf1p kinase; forms cytoplasmic foci upon DNA replication stress
0.110 1.46E-10 YGL074C_d YGL074C_d het Dubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential HSF1 gene encoding heat shock transcription factor
0.106 7.02E-10 YLR167W RPS31 het Fusion protein cleaved to yield ribosomal protein S31 and ubiquitin; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; interacts genetically with translation factor eIF2B; homologous to mammalian ribosomal protein S27A, no bacterial homolog
0.105 1.10E-9 YHR164C DNA2 het Tripartite DNA replication factor; has single-stranded DNA-dependent ATPase, ATP-dependent nuclease, and helicase activities; required for Okazaki fragment processing; involved in DNA repair; cell-cycle dependent localization; forms nuclear foci upon DNA replication stress
0.104 1.58E-9 YJL090C DPB11 het DNA replication initiation protein; loads DNA pol epsilon onto pre-replication complexes at origins; checkpoint sensor recruited to stalled replication forks by the checkpoint clamp complex where it activates Mec1p; ortholog of human TopBP1; forms nuclear foci upon DNA replication stress
0.103 2.04E-9 YOL034W SMC5 het Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; binds single-stranded DNA and has ATPase activity; S. pombe homolog forms a heterodimer with S. pombe Rad18p that is involved in DNA repair