YML106W / URA5

Major orotate phosphoribosyltransferase (OPRTase) isozyme; catalyzes the fifth enzymatic step in de novo biosynthesis of pyrimidines, converting orotate into orotidine-5'-phosphate; URA5 has a paralog, URA10, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YML106W / URA5

Click on Significant Values for Screen Details ID:SGTC_58|Compound:0957-0393|FD-Score:-4.46|P-value:2.33E-6 ID:SGTC_59|Compound:0330-0160|FD-Score:3.30|P-value:3.72E-4 ID:SGTC_103|Compound:0844-0104|FD-Score:-3.04|P-value:9.06E-4 ID:SGTC_159|Compound:3970-0959|FD-Score:-3.17|P-value:5.79E-4 ID:SGTC_221|Compound:trifluoperazine|FD-Score:8.24|P-value:1.65E-17 ID:SGTC_298|Compound:1391-0729|FD-Score:4.43|P-value:2.92E-6 ID:SGTC_309|Compound:k408-0051|FD-Score:3.28|P-value:4.03E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:-4.62|P-value:1.07E-6 ID:SGTC_514|Compound:4466-0038|FD-Score:3.57|P-value:1.31E-4 ID:SGTC_691|Compound:1548-0686|FD-Score:-4.11|P-value:1.25E-5 ID:SGTC_702|Compound:1488-0190|FD-Score:4.51|P-value:1.95E-6 ID:SGTC_703|Compound:3875-0075|FD-Score:-3.19|P-value:5.25E-4 ID:SGTC_763|Compound:3453-1109|FD-Score:3.66|P-value:9.19E-5 ID:SGTC_1055|Compound:3448-0672|FD-Score:3.64|P-value:9.88E-5 ID:SGTC_1061|Compound:3448-9325|FD-Score:3.13|P-value:6.92E-4 ID:SGTC_1257|Compound:0669-0121|FD-Score:3.85|P-value:4.10E-5 ID:SGTC_1324|Compound:1418-0026|FD-Score:3.09|P-value:7.93E-4 ID:SGTC_1334|Compound:1486-0610|FD-Score:3.67|P-value:8.77E-5 ID:SGTC_1383|Compound:3049-0032|FD-Score:3.68|P-value:8.24E-5 ID:SGTC_1431|Compound:4049-0260|FD-Score:3.17|P-value:5.92E-4 ID:SGTC_1589|Compound:2',5'-dimethoxyflavone|FD-Score:-3.16|P-value:5.99E-4 ID:SGTC_1707|Compound:st032323|FD-Score:3.21|P-value:5.21E-4 ID:SGTC_1712|Compound:st032288|FD-Score:3.63|P-value:1.01E-4 ID:SGTC_1714|Compound:st033236|FD-Score:4.33|P-value:4.65E-6 ID:SGTC_1715|Compound:st032160|FD-Score:3.89|P-value:3.39E-5 ID:SGTC_1734|Compound:st037168|FD-Score:3.23|P-value:4.76E-4 ID:SGTC_1745|Compound:st081495|FD-Score:7.87|P-value:3.99E-16 ID:SGTC_1754|Compound:st042065|FD-Score:8.98|P-value:1.90E-20 ID:SGTC_1760|Compound:st045453|FD-Score:6.41|P-value:2.58E-11 ID:SGTC_1797|Compound:st048695|FD-Score:3.64|P-value:9.99E-5 ID:SGTC_1800|Compound:st050079|FD-Score:3.15|P-value:6.35E-4 ID:SGTC_1803|Compound:st050068|FD-Score:3.78|P-value:5.41E-5 ID:SGTC_1837|Compound:st055472|FD-Score:3.10|P-value:7.51E-4 ID:SGTC_2142|Compound:5622915|FD-Score:-3.17|P-value:5.70E-4 ID:SGTC_2276|Compound:7947513|FD-Score:3.47|P-value:1.95E-4 ID:SGTC_2277|Compound:7947813|FD-Score:3.51|P-value:1.66E-4 ID:SGTC_2319|Compound:6033012|FD-Score:3.34|P-value:3.12E-4 ID:SGTC_2406|Compound:ethylparaben|FD-Score:-4.12|P-value:1.19E-5 ID:SGTC_2493|Compound:resveratrol|FD-Score:3.06|P-value:8.89E-4 ID:SGTC_2509|Compound:methyl 7-deshydroxypyrogallin-4-carboxylate|FD-Score:-3.92|P-value:2.84E-5 ID:SGTC_2522|Compound:biochanin a|FD-Score:-3.37|P-value:2.71E-4 ID:SGTC_2523|Compound:spermine|FD-Score:3.16|P-value:6.09E-4 ID:SGTC_2651|Compound:1327-0036|FD-Score:6.66|P-value:4.48E-12 ID:SGTC_2749|Compound:bexarotene|FD-Score:3.10|P-value:7.61E-4 ID:SGTC_2876|Compound:9059903|FD-Score:3.05|P-value:9.09E-4 ID:SGTC_2934|Compound:9036370|FD-Score:-3.40|P-value:2.40E-4 ID:SGTC_3028|Compound:9087778|FD-Score:3.37|P-value:2.84E-4 ID:SGTC_3037|Compound:9091026|FD-Score:3.95|P-value:2.71E-5 ID:SGTC_3083|Compound:9117465|FD-Score:-3.27|P-value:4.00E-4 ID:SGTC_3311|Compound:9131146|FD-Score:4.73|P-value:6.49E-7 ID:SGTC_58|Compound:0957-0393|FD-Score:-4.46|P-value:2.33E-6 ID:SGTC_59|Compound:0330-0160|FD-Score:3.30|P-value:3.72E-4 ID:SGTC_103|Compound:0844-0104|FD-Score:-3.04|P-value:9.06E-4 ID:SGTC_159|Compound:3970-0959|FD-Score:-3.17|P-value:5.79E-4 ID:SGTC_221|Compound:trifluoperazine|FD-Score:8.24|P-value:1.65E-17 ID:SGTC_298|Compound:1391-0729|FD-Score:4.43|P-value:2.92E-6 ID:SGTC_309|Compound:k408-0051|FD-Score:3.28|P-value:4.03E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:-4.62|P-value:1.07E-6 ID:SGTC_514|Compound:4466-0038|FD-Score:3.57|P-value:1.31E-4 ID:SGTC_691|Compound:1548-0686|FD-Score:-4.11|P-value:1.25E-5 ID:SGTC_702|Compound:1488-0190|FD-Score:4.51|P-value:1.95E-6 ID:SGTC_703|Compound:3875-0075|FD-Score:-3.19|P-value:5.25E-4 ID:SGTC_763|Compound:3453-1109|FD-Score:3.66|P-value:9.19E-5 ID:SGTC_1055|Compound:3448-0672|FD-Score:3.64|P-value:9.88E-5 ID:SGTC_1061|Compound:3448-9325|FD-Score:3.13|P-value:6.92E-4 ID:SGTC_1257|Compound:0669-0121|FD-Score:3.85|P-value:4.10E-5 ID:SGTC_1324|Compound:1418-0026|FD-Score:3.09|P-value:7.93E-4 ID:SGTC_1334|Compound:1486-0610|FD-Score:3.67|P-value:8.77E-5 ID:SGTC_1383|Compound:3049-0032|FD-Score:3.68|P-value:8.24E-5 ID:SGTC_1431|Compound:4049-0260|FD-Score:3.17|P-value:5.92E-4 ID:SGTC_1589|Compound:2',5'-dimethoxyflavone|FD-Score:-3.16|P-value:5.99E-4 ID:SGTC_1707|Compound:st032323|FD-Score:3.21|P-value:5.21E-4 ID:SGTC_1712|Compound:st032288|FD-Score:3.63|P-value:1.01E-4 ID:SGTC_1714|Compound:st033236|FD-Score:4.33|P-value:4.65E-6 ID:SGTC_1715|Compound:st032160|FD-Score:3.89|P-value:3.39E-5 ID:SGTC_1734|Compound:st037168|FD-Score:3.23|P-value:4.76E-4 ID:SGTC_1745|Compound:st081495|FD-Score:7.87|P-value:3.99E-16 ID:SGTC_1754|Compound:st042065|FD-Score:8.98|P-value:1.90E-20 ID:SGTC_1760|Compound:st045453|FD-Score:6.41|P-value:2.58E-11 ID:SGTC_1797|Compound:st048695|FD-Score:3.64|P-value:9.99E-5 ID:SGTC_1800|Compound:st050079|FD-Score:3.15|P-value:6.35E-4 ID:SGTC_1803|Compound:st050068|FD-Score:3.78|P-value:5.41E-5 ID:SGTC_1837|Compound:st055472|FD-Score:3.10|P-value:7.51E-4 ID:SGTC_2142|Compound:5622915|FD-Score:-3.17|P-value:5.70E-4 ID:SGTC_2276|Compound:7947513|FD-Score:3.47|P-value:1.95E-4 ID:SGTC_2277|Compound:7947813|FD-Score:3.51|P-value:1.66E-4 ID:SGTC_2319|Compound:6033012|FD-Score:3.34|P-value:3.12E-4 ID:SGTC_2406|Compound:ethylparaben|FD-Score:-4.12|P-value:1.19E-5 ID:SGTC_2493|Compound:resveratrol|FD-Score:3.06|P-value:8.89E-4 ID:SGTC_2509|Compound:methyl 7-deshydroxypyrogallin-4-carboxylate|FD-Score:-3.92|P-value:2.84E-5 ID:SGTC_2522|Compound:biochanin a|FD-Score:-3.37|P-value:2.71E-4 ID:SGTC_2523|Compound:spermine|FD-Score:3.16|P-value:6.09E-4 ID:SGTC_2651|Compound:1327-0036|FD-Score:6.66|P-value:4.48E-12 ID:SGTC_2749|Compound:bexarotene|FD-Score:3.10|P-value:7.61E-4 ID:SGTC_2876|Compound:9059903|FD-Score:3.05|P-value:9.09E-4 ID:SGTC_2934|Compound:9036370|FD-Score:-3.40|P-value:2.40E-4 ID:SGTC_3028|Compound:9087778|FD-Score:3.37|P-value:2.84E-4 ID:SGTC_3037|Compound:9091026|FD-Score:3.95|P-value:2.71E-5 ID:SGTC_3083|Compound:9117465|FD-Score:-3.27|P-value:4.00E-4 ID:SGTC_3311|Compound:9131146|FD-Score:4.73|P-value:6.49E-7

Top fitness defect scores for YML106W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1754 st042065 8.98 16.1 µM 1.90E-20 NEO1-PIK1 39 1.37E-19
2 SGTC_221 trifluoperazine 8.24 10.3 µM 1.65E-17 NEO1-PIK1 81 8.75E-17
3 SGTC_1745 st081495 7.87 74.0 µM 3.99E-16 RNA processing & uracil transport 14 1.82E-15
4 SGTC_2651 1327-0036 6.66 5.0 µM 4.48E-12 54 1.33E-11
5 SGTC_1760 st045453 6.41 10.4 µM 2.58E-11 NEO1-PIK1 75 7.09E-11
6 SGTC_3311 9131146 4.73 2.9 µM 6.49E-7 cell wall 89 1.13E-6
7 SGTC_702 1488-0190 4.51 94.0 µM 1.95E-6 amide catabolism 8 3.23E-6
8 SGTC_298 1391-0729 4.43 10.0 µM 2.92E-6 calcium & mitochondrial duress 15 4.74E-6
9 SGTC_1714 st033236 4.33 13.6 µM 4.65E-6 16 7.40E-6
10 SGTC_3037 9091026 3.95 49.5 µM 2.71E-5 NEO1-PIK1 124 3.98E-5
11 SGTC_1715 st032160 3.89 10.2 µM 3.39E-5 43 4.94E-5
12 SGTC_1257 0669-0121 3.85 16.0 µM 4.10E-5 31 5.92E-5
13 SGTC_1803 st050068 3.78 52.4 µM 5.41E-5 NEO1-PIK1 195 7.73E-5
14 SGTC_1383 3049-0032 3.68 20.7 µM 8.24E-5 60S ribosome export 26 1.15E-4
15 SGTC_1334 1486-0610 3.67 236.0 µM 8.77E-5 60S ribosome export 26 1.23E-4
16 SGTC_763 3453-1109 3.66 128.0 µM 9.19E-5 28 1.28E-4
17 SGTC_1055 3448-0672 3.64 86.0 µM 9.88E-5 39 1.37E-4
18 SGTC_1797 st048695 3.64 56.8 µM 9.99E-5 NEO1 78 1.39E-4
19 SGTC_1712 st032288 3.63 53.4 µM 1.01E-4 calcium & mitochondrial duress 215 1.40E-4
20 SGTC_514 4466-0038 3.57 2.6 nM 1.31E-4 cell wall 180 1.81E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.352 1.26E-98 YLR020C YEH2 hom Steryl ester hydrolase, catalyzes steryl ester hydrolysis at the plasma membrane; involved in sterol metabolism
0.343 3.54E-93 YHR108W GGA2 hom Protein that interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner to facilitate traffic through the late Golgi; binds phosphatidylinositol 4-phosphate, which plays a role in TGN localization; has homology to gamma-adaptin
0.343 3.57E-93 YKL191W DPH2 hom Protein required, along with Dph1p, Kti11p, Jjj3p, and Dph5p, for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); may act in a complex with Dph1p and Kti11p
0.320 8.21E-81 YAR042W SWH1 hom Protein similar to mammalian oxysterol-binding protein; contains ankyrin repeats; localizes to the Golgi and the nucleus-vacuole junction
0.320 8.83E-81 YPR091C NVJ2 hom Lipid-binding ER protein, enriched at nucleus-vacuolar junctions (NVJ); may be involved in sterol metabolism or signaling at the NVJ; contains a synaptotagmin-like-mitochondrial- lipid binding protein (SMP) domain; binds phosphatidylinositols and other lipids in a large-scale study; may interact with ribosomes, based on co-purification experiments
0.304 1.36E-72 YML097C VPS9 hom A guanine nucleotide exchange factor involved in vesicle-mediated vacuolar protein transport; specifically stimulates the intrinsic guanine nucleotide exchange activity of Vps21p/Rab5: similar to mammalian ras inhibitors; binds ubiquitin
0.290 3.36E-66 YBR246W RRT2 hom Protein required for last step of diphthamide biosynthesis; deletion leads to resistance to sordarin and accumulation of diphthine; WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rtt10p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription
0.289 2.02E-65 YJL154C VPS35 hom Endosomal subunit of membrane-associated retromer complex required for retrograde transport; receptor that recognizes retrieval signals on cargo proteins, forms subcomplex with Vps26p and Vps29p that selects cargo proteins for retrieval; interacts with Ypt7p
0.288 3.14E-65 YJL052W TDH1 hom Glyceraldehyde-3-phosphate dehydrogenase, isozyme 1; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; protein abundance increases in response to DNA replication stress
0.281 5.71E-62 YBR245C ISW1 hom ATPase subunit of imitation-switch (ISWI) class chromatin remodelers; ATPase; forms a complex with Ioc3p (Isw1a), and a complex with Ioc2p and Ioc4p (Isw1b); Isw1a and Isw1b have partially overlapping and distinct roles, Isw1a involved in repression of transcription initiation and Isw1b involved in regulation of transcription elongation; Isw1b recruited to open reading frames by H3K36 methylation and acts with Chd1p to prevent trans-histone exchange over coding regions
0.274 1.23E-58 YGL019W CKB1 hom Beta regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases
0.273 1.31E-58 YML037C_p YML037C_p hom Putative protein of unknown function with some characteristics of a transcriptional activator; may be a target of Dbf2p-Mob1p kinase; GFP-fusion protein co-localizes with clathrin-coated vesicles; YML037C is not an essential gene
0.272 4.80E-58 YNL183C NPR1 hom Protein kinase that stabilizes several plasma membrane amino acid transporters by antagonizing their ubiquitin-mediated degradation; phosphorylates Aly2p; negatively regulates Ldb19p-mediated endocytosis through phosphorylation of Ldb19p, which prevents its association with the plasma membrane; Npr1p activity is negatively regulated via phosphorylation by the TOR complex
0.263 2.44E-54 YGR202C PCT1 hom Cholinephosphate cytidylyltransferase; a rate-determining enzyme of the CDP-choline pathway for phosphatidylcholine synthesis, inhibited by Sec14p, activated upon lipid-binding; contains an element within the regulatory domain involved in both silencing and activation of enzymatic activity
0.259 1.32E-52 YDR084C TVP23 hom Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern