YML118W / NGL3

3'-5' exonuclease specific for poly-A RNAs; has a domain similar to a magnesium-dependent endonuclease motif in mRNA deadenylase Ccr4p; similar to Ngl1p; NGL3 has a paralog, NGL2, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YML118W / NGL3

Click on Significant Values for Screen Details ID:SGTC_317|Compound:4495-0002|FD-Score:3.64|P-value:3.31E-5 ID:SGTC_437|Compound:s-farnesyl-l-cysteine methyl ester|FD-Score:3.36|P-value:1.15E-4 ID:SGTC_451|Compound:chloral hydrate|FD-Score:3.54|P-value:5.10E-5 ID:SGTC_505|Compound:gw-5074|FD-Score:3.42|P-value:8.92E-5 ID:SGTC_953|Compound:1188-0045|FD-Score:4.41|P-value:6.78E-7 ID:SGTC_996|Compound:3970-0799|FD-Score:37.10|P-value:0 ID:SGTC_998|Compound:3996-0085|FD-Score:5.49|P-value:9.77E-10 ID:SGTC_1125|Compound:tnp00248|FD-Score:3.44|P-value:7.99E-5 ID:SGTC_1333|Compound:1482-0047|FD-Score:3.29|P-value:1.54E-4 ID:SGTC_1440|Compound:4100-1550|FD-Score:-3.58|P-value:5.96E-5 ID:SGTC_1546|Compound:5-methoxyflavanone|FD-Score:2.98|P-value:5.36E-4 ID:SGTC_1563|Compound:conessine|FD-Score:3.97|P-value:6.82E-6 ID:SGTC_1566|Compound:6,7-dimethoxy-4-ethylcoumarin|FD-Score:2.93|P-value:6.35E-4 ID:SGTC_1569|Compound:carminic acid|FD-Score:3.56|P-value:4.76E-5 ID:SGTC_1572|Compound:epiandrosterone|FD-Score:3.96|P-value:7.23E-6 ID:SGTC_1637|Compound:st007924|FD-Score:-2.95|P-value:7.97E-4 ID:SGTC_1679|Compound:4130-1276|FD-Score:3.83|P-value:1.34E-5 ID:SGTC_1753|Compound:st043925|FD-Score:2.85|P-value:8.65E-4 ID:SGTC_1777|Compound:st045864|FD-Score:3.34|P-value:1.22E-4 ID:SGTC_1781|Compound:st038725|FD-Score:-3.21|P-value:2.94E-4 ID:SGTC_2045|Compound:5106652|FD-Score:3.09|P-value:3.51E-4 ID:SGTC_2281|Compound:7943571|FD-Score:2.81|P-value:9.88E-4 ID:SGTC_2287|Compound:7945887|FD-Score:2.84|P-value:8.96E-4 ID:SGTC_2345|Compound:9052315|FD-Score:-2.98|P-value:7.07E-4 ID:SGTC_2383|Compound:9036125|FD-Score:3.59|P-value:4.12E-5 ID:SGTC_2386|Compound:9075165|FD-Score:3.04|P-value:4.16E-4 ID:SGTC_2387|Compound:9077560|FD-Score:-3.38|P-value:1.46E-4 ID:SGTC_2427|Compound:5545055|FD-Score:3.59|P-value:4.12E-5 ID:SGTC_2693|Compound:st077581|FD-Score:3.63|P-value:3.40E-5 ID:SGTC_2736|Compound:butenafine|FD-Score:4.09|P-value:3.84E-6 ID:SGTC_2876|Compound:9059903|FD-Score:3.55|P-value:4.99E-5 ID:SGTC_2909|Compound:7941780|FD-Score:3.39|P-value:9.87E-5 ID:SGTC_2978|Compound:9011784|FD-Score:3.64|P-value:3.32E-5 ID:SGTC_3021|Compound:9087658|FD-Score:3.66|P-value:3.00E-5 ID:SGTC_3025|Compound:9087895|FD-Score:3.82|P-value:1.41E-5 ID:SGTC_3035|Compound:9091303|FD-Score:-3.19|P-value:3.09E-4 ID:SGTC_3317|Compound:9138495|FD-Score:-3.15|P-value:3.67E-4 ID:SGTC_317|Compound:4495-0002|FD-Score:3.64|P-value:3.31E-5 ID:SGTC_437|Compound:s-farnesyl-l-cysteine methyl ester|FD-Score:3.36|P-value:1.15E-4 ID:SGTC_451|Compound:chloral hydrate|FD-Score:3.54|P-value:5.10E-5 ID:SGTC_505|Compound:gw-5074|FD-Score:3.42|P-value:8.92E-5 ID:SGTC_953|Compound:1188-0045|FD-Score:4.41|P-value:6.78E-7 ID:SGTC_996|Compound:3970-0799|FD-Score:37.10|P-value:0 ID:SGTC_998|Compound:3996-0085|FD-Score:5.49|P-value:9.77E-10 ID:SGTC_1125|Compound:tnp00248|FD-Score:3.44|P-value:7.99E-5 ID:SGTC_1333|Compound:1482-0047|FD-Score:3.29|P-value:1.54E-4 ID:SGTC_1440|Compound:4100-1550|FD-Score:-3.58|P-value:5.96E-5 ID:SGTC_1546|Compound:5-methoxyflavanone|FD-Score:2.98|P-value:5.36E-4 ID:SGTC_1563|Compound:conessine|FD-Score:3.97|P-value:6.82E-6 ID:SGTC_1566|Compound:6,7-dimethoxy-4-ethylcoumarin|FD-Score:2.93|P-value:6.35E-4 ID:SGTC_1569|Compound:carminic acid|FD-Score:3.56|P-value:4.76E-5 ID:SGTC_1572|Compound:epiandrosterone|FD-Score:3.96|P-value:7.23E-6 ID:SGTC_1637|Compound:st007924|FD-Score:-2.95|P-value:7.97E-4 ID:SGTC_1679|Compound:4130-1276|FD-Score:3.83|P-value:1.34E-5 ID:SGTC_1753|Compound:st043925|FD-Score:2.85|P-value:8.65E-4 ID:SGTC_1777|Compound:st045864|FD-Score:3.34|P-value:1.22E-4 ID:SGTC_1781|Compound:st038725|FD-Score:-3.21|P-value:2.94E-4 ID:SGTC_2045|Compound:5106652|FD-Score:3.09|P-value:3.51E-4 ID:SGTC_2281|Compound:7943571|FD-Score:2.81|P-value:9.88E-4 ID:SGTC_2287|Compound:7945887|FD-Score:2.84|P-value:8.96E-4 ID:SGTC_2345|Compound:9052315|FD-Score:-2.98|P-value:7.07E-4 ID:SGTC_2383|Compound:9036125|FD-Score:3.59|P-value:4.12E-5 ID:SGTC_2386|Compound:9075165|FD-Score:3.04|P-value:4.16E-4 ID:SGTC_2387|Compound:9077560|FD-Score:-3.38|P-value:1.46E-4 ID:SGTC_2427|Compound:5545055|FD-Score:3.59|P-value:4.12E-5 ID:SGTC_2693|Compound:st077581|FD-Score:3.63|P-value:3.40E-5 ID:SGTC_2736|Compound:butenafine|FD-Score:4.09|P-value:3.84E-6 ID:SGTC_2876|Compound:9059903|FD-Score:3.55|P-value:4.99E-5 ID:SGTC_2909|Compound:7941780|FD-Score:3.39|P-value:9.87E-5 ID:SGTC_2978|Compound:9011784|FD-Score:3.64|P-value:3.32E-5 ID:SGTC_3021|Compound:9087658|FD-Score:3.66|P-value:3.00E-5 ID:SGTC_3025|Compound:9087895|FD-Score:3.82|P-value:1.41E-5 ID:SGTC_3035|Compound:9091303|FD-Score:-3.19|P-value:3.09E-4 ID:SGTC_3317|Compound:9138495|FD-Score:-3.15|P-value:3.67E-4

Top fitness defect scores for YML118W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_996 3970-0799 37.10 1.8 µM 0 1 4.62E-301
2 SGTC_998 3996-0085 5.49 177.0 µM 9.77E-10 5 1.97E-8
3 SGTC_953 1188-0045 4.41 9.7 µM 6.78E-7 60S ribosome export 19 5.06E-6
4 SGTC_2736 butenafine 4.09 14.7 µM 3.84E-6 27 2.20E-5
5 SGTC_1563 conessine 3.97 56.1 µM 6.82E-6 16 3.58E-5
6 SGTC_1572 epiandrosterone 3.96 68.9 µM 7.23E-6 25 3.77E-5
7 SGTC_1679 4130-1276 3.83 6.7 µM 1.34E-5 Golgi 67 6.35E-5
8 SGTC_3025 9087895 3.82 49.5 µM 1.41E-5 PDR1 42 6.62E-5
9 SGTC_3021 9087658 3.66 49.5 µM 3.00E-5 9 1.26E-4
10 SGTC_317 4495-0002 3.64 170.0 µM 3.31E-5 14 1.37E-4
11 SGTC_2978 9011784 3.64 43.0 µM 3.32E-5 42 1.37E-4
12 SGTC_2693 st077581 3.63 40.7 µM 3.40E-5 50 1.40E-4
13 SGTC_2383 9036125 3.59 200.0 µM 4.12E-5 10 1.65E-4
14 SGTC_2427 5545055 3.59 42.8 µM 4.12E-5 25 1.65E-4
15 SGTC_1569 carminic acid 3.56 40.6 µM 4.76E-5 30 1.86E-4
16 SGTC_2876 9059903 3.55 26.0 µM 4.99E-5 20 1.94E-4
17 SGTC_451 chloral hydrate 3.54 100.0 µM 5.10E-5 73 1.97E-4
18 SGTC_1125 tnp00248 3.44 1.3 µM 7.99E-5 57 2.89E-4
19 SGTC_505 gw-5074 3.42 63.9 µM 8.92E-5 77 3.18E-4
20 SGTC_2909 7941780 3.39 10.5 µM 9.87E-5 40 3.46E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.652 0 YDL099W BUG1 hom Cis-golgi localized protein involved in ER to Golgi transport; forms a complex with the mammalian GRASP65 homolog, Grh1p; mutants are compromised for the fusion of ER-derived vesicles with Golgi membranes
0.598 9.88E-324 YDR505C PSP1 hom Asn and gln rich protein of unknown function; high-copy suppressor of POL1 (DNA polymerase alpha) and partial suppressor of CDC2 (polymerase delta) and CDC6 (pre-RC loading factor) mutations; overexpression results in growth inhibition; PSP1 has a paralog, YLR177W, that arose from the whole genome duplication
0.546 2.74E-260 YGL040C HEM2 het Aminolevulinate dehydratase, a homo-octameric enzyme, catalyzes the conversion of 5-aminolevulinate to porphobilinogen, the second step in heme biosynthesis; enzymatic activity is zinc-dependent; localizes to the cytoplasm and nucleus
0.541 9.54E-255 YGL222C EDC1 hom RNA-binding protein that activates mRNA decapping directly; binds to the mRNA substrate and enhances the activity of the decapping proteins Dcp1p and Dcp2p; has a role in translation during heat stress; protein becomes more abundant and forms cytoplasmic foci in response to DNA replication stress
0.541 2.46E-254 YOR037W CYC2 hom Mitochondrial peripheral inner membrane protein, contains a FAD cofactor in a domain exposed in the intermembrane space; exhibits redox activity in vitro; likely participates in ligation of heme to acytochromes c and c1 (Cyc1p and Cyt1p)
0.499 2.37E-210 YGL205W POX1 hom Fatty-acyl coenzyme A oxidase, involved in the fatty acid beta-oxidation pathway; localized to the peroxisomal matrix
0.461 1.17E-175 YGL083W SCY1 hom Putative kinase, suppressor of GTPase mutant, similar to bovine rhodopsin kinase
0.454 6.74E-170 YBR067C TIP1 hom Major cell wall mannoprotein with possible lipase activity; transcription is induced by heat- and cold-shock; member of the Srp1p/Tip1p family of serine-alanine-rich proteins
0.438 8.51E-157 YNR033W ABZ1 hom Para-aminobenzoate (PABA) synthase; has similarity to Escherichia coli PABA synthase components PabA and PabB; required for the synthesis of para-aminobenzoic acid, an important intermediate for folate and ubiquinone Q biosynthesis; protein abundance increases in response to DNA replication stress
0.428 4.45E-149 YGR035C_p YGR035C_p hom Putative protein of unknown function, potential Cdc28p substrate; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance
0.404 1.20E-131 YDL186W_p YDL186W_p hom Putative protein of unknown function; YDL186W is not an essential gene
0.403 2.66E-131 YGL096W TOS8 hom Homeodomain-containing protein and putative transcription factor; found associated with chromatin; target of SBF transcription factor; induced during meiosis and under cell-damaging conditions; TOS8 has a paralog, CUP9, that arose from the whole genome duplication
0.402 2.13E-130 YDL177C_p YDL177C_p hom Putative protein of unknown function; similar to the mouse IMPACT gene; YDL177C is not an essential gene
0.401 1.35E-129 YJR014W TMA22 hom Protein of unknown function; associates with ribosomes and has a putative RNA binding domain; interacts with Tma20p; similar to human GRAP and human DRP1, which interacts with human Tma20p homolog MCT-1; protein abundance increases in response to DNA replication stress
0.398 7.47E-128 YJL149W DAS1 hom Putative SCF ubiquitin ligase F-box protein; interacts physically with both Cdc53p and Skp1 and genetically with CDC34; similar to putative F-box protein YDR131C