YML130C / ERO1

Thiol oxidase required for oxidative protein folding in the ER; essential for maintaining proper redox balance in ER; feedback regulation of Ero1p occurs via reduction and oxidation of Ero1p regulatory bonds; reduced Pdi1p activates Ero1p by direct reduction of Ero1p regulatory bonds; depletion of thiol substrates and accumulation of oxidized Pdi1p results in inactivation of Ero1p by both Pdi1p-mediated oxidation and autonomous oxidation of Ero1p regulatory bonds

Zygosity: Heterozygous strain
fixedexpanded
Profile for YML130C / ERO1

Click on Significant Values for Screen Details ID:SGTC_16|Compound:erodoxin|FD-Score:30.90|P-value:1.38E-270 ID:SGTC_28|Compound:pentachloropyridine N-oxide|FD-Score:2.80|P-value:7.68E-4 ID:SGTC_116|Compound:0155-0182|FD-Score:-3.28|P-value:8.67E-5 ID:SGTC_182|Compound:3448-4371|FD-Score:2.82|P-value:6.92E-4 ID:SGTC_272|Compound:doxorubicin|FD-Score:2.97|P-value:3.78E-4 ID:SGTC_291|Compound:4130-1278|FD-Score:-3.63|P-value:1.63E-5 ID:SGTC_356|Compound:1634-0012|FD-Score:2.91|P-value:4.94E-4 ID:SGTC_405|Compound:ebelactone b|FD-Score:2.94|P-value:4.43E-4 ID:SGTC_513|Compound:4245-1575|FD-Score:5.42|P-value:3.89E-10 ID:SGTC_644|Compound:1498-1197|FD-Score:2.78|P-value:8.20E-4 ID:SGTC_1008|Compound:4106-0037|FD-Score:2.91|P-value:4.86E-4 ID:SGTC_1130|Compound:3454-2858|FD-Score:3.07|P-value:2.50E-4 ID:SGTC_1184|Compound:1269-1718|FD-Score:4.37|P-value:3.48E-7 ID:SGTC_1239|Compound:0337-0584|FD-Score:36.90|P-value:0 ID:SGTC_1240|Compound:0356-0016|FD-Score:-3.80|P-value:6.95E-6 ID:SGTC_1384|Compound:3089-0840|FD-Score:-2.95|P-value:3.67E-4 ID:SGTC_1399|Compound:3562-5772|FD-Score:-4.11|P-value:1.31E-6 ID:SGTC_1462|Compound:k081-0014|FD-Score:-3.56|P-value:2.30E-5 ID:SGTC_1558|Compound:st069300|FD-Score:3.17|P-value:1.67E-4 ID:SGTC_1587|Compound:estradiol valerate|FD-Score:2.81|P-value:7.35E-4 ID:SGTC_1838|Compound:st055369|FD-Score:-3.24|P-value:1.03E-4 ID:SGTC_1908|Compound:st060353|FD-Score:3.46|P-value:4.48E-5 ID:SGTC_1909|Compound:st060837|FD-Score:2.83|P-value:6.71E-4 ID:SGTC_1917|Compound:st066507|FD-Score:3.13|P-value:1.96E-4 ID:SGTC_1918|Compound:oxolan-2-ylmethyl 3-bromobenzoate|FD-Score:7.40|P-value:2.03E-17 ID:SGTC_1947|Compound:st076332|FD-Score:-3.13|P-value:1.67E-4 ID:SGTC_2070|Compound:5230947|FD-Score:-3.45|P-value:3.95E-5 ID:SGTC_2082|Compound:5219693|FD-Score:-2.81|P-value:6.54E-4 ID:SGTC_2216|Compound:7189485|FD-Score:2.91|P-value:4.99E-4 ID:SGTC_2261|Compound:9010538|FD-Score:2.97|P-value:3.80E-4 ID:SGTC_2345|Compound:9052315|FD-Score:-2.75|P-value:8.18E-4 ID:SGTC_2360|Compound:9040895|FD-Score:-2.90|P-value:4.45E-4 ID:SGTC_2565|Compound:isoliquiritigenin|FD-Score:-3.51|P-value:2.97E-5 ID:SGTC_2566|Compound:crystal violet carbinol|FD-Score:2.86|P-value:6.02E-4 ID:SGTC_2715|Compound:st079500|FD-Score:-3.32|P-value:7.44E-5 ID:SGTC_2754|Compound:granisetron|FD-Score:2.77|P-value:8.44E-4 ID:SGTC_2794|Compound:7785382|FD-Score:3.17|P-value:1.63E-4 ID:SGTC_2943|Compound:9068529|FD-Score:-3.21|P-value:1.20E-4 ID:SGTC_3210|Compound:9128157|FD-Score:-3.09|P-value:2.00E-4 ID:SGTC_3214|Compound:9129694|FD-Score:4.67|P-value:5.94E-8 ID:SGTC_3215|Compound:9129942|FD-Score:3.28|P-value:1.01E-4 ID:SGTC_3340|Compound:9146634|FD-Score:2.83|P-value:6.88E-4 ID:SGTC_16|Compound:erodoxin|FD-Score:30.90|P-value:1.38E-270 ID:SGTC_28|Compound:pentachloropyridine N-oxide|FD-Score:2.80|P-value:7.68E-4 ID:SGTC_116|Compound:0155-0182|FD-Score:-3.28|P-value:8.67E-5 ID:SGTC_182|Compound:3448-4371|FD-Score:2.82|P-value:6.92E-4 ID:SGTC_272|Compound:doxorubicin|FD-Score:2.97|P-value:3.78E-4 ID:SGTC_291|Compound:4130-1278|FD-Score:-3.63|P-value:1.63E-5 ID:SGTC_356|Compound:1634-0012|FD-Score:2.91|P-value:4.94E-4 ID:SGTC_405|Compound:ebelactone b|FD-Score:2.94|P-value:4.43E-4 ID:SGTC_513|Compound:4245-1575|FD-Score:5.42|P-value:3.89E-10 ID:SGTC_644|Compound:1498-1197|FD-Score:2.78|P-value:8.20E-4 ID:SGTC_1008|Compound:4106-0037|FD-Score:2.91|P-value:4.86E-4 ID:SGTC_1130|Compound:3454-2858|FD-Score:3.07|P-value:2.50E-4 ID:SGTC_1184|Compound:1269-1718|FD-Score:4.37|P-value:3.48E-7 ID:SGTC_1239|Compound:0337-0584|FD-Score:36.90|P-value:0 ID:SGTC_1240|Compound:0356-0016|FD-Score:-3.80|P-value:6.95E-6 ID:SGTC_1384|Compound:3089-0840|FD-Score:-2.95|P-value:3.67E-4 ID:SGTC_1399|Compound:3562-5772|FD-Score:-4.11|P-value:1.31E-6 ID:SGTC_1462|Compound:k081-0014|FD-Score:-3.56|P-value:2.30E-5 ID:SGTC_1558|Compound:st069300|FD-Score:3.17|P-value:1.67E-4 ID:SGTC_1587|Compound:estradiol valerate|FD-Score:2.81|P-value:7.35E-4 ID:SGTC_1838|Compound:st055369|FD-Score:-3.24|P-value:1.03E-4 ID:SGTC_1908|Compound:st060353|FD-Score:3.46|P-value:4.48E-5 ID:SGTC_1909|Compound:st060837|FD-Score:2.83|P-value:6.71E-4 ID:SGTC_1917|Compound:st066507|FD-Score:3.13|P-value:1.96E-4 ID:SGTC_1918|Compound:oxolan-2-ylmethyl 3-bromobenzoate|FD-Score:7.40|P-value:2.03E-17 ID:SGTC_1947|Compound:st076332|FD-Score:-3.13|P-value:1.67E-4 ID:SGTC_2070|Compound:5230947|FD-Score:-3.45|P-value:3.95E-5 ID:SGTC_2082|Compound:5219693|FD-Score:-2.81|P-value:6.54E-4 ID:SGTC_2216|Compound:7189485|FD-Score:2.91|P-value:4.99E-4 ID:SGTC_2261|Compound:9010538|FD-Score:2.97|P-value:3.80E-4 ID:SGTC_2345|Compound:9052315|FD-Score:-2.75|P-value:8.18E-4 ID:SGTC_2360|Compound:9040895|FD-Score:-2.90|P-value:4.45E-4 ID:SGTC_2565|Compound:isoliquiritigenin|FD-Score:-3.51|P-value:2.97E-5 ID:SGTC_2566|Compound:crystal violet carbinol|FD-Score:2.86|P-value:6.02E-4 ID:SGTC_2715|Compound:st079500|FD-Score:-3.32|P-value:7.44E-5 ID:SGTC_2754|Compound:granisetron|FD-Score:2.77|P-value:8.44E-4 ID:SGTC_2794|Compound:7785382|FD-Score:3.17|P-value:1.63E-4 ID:SGTC_2943|Compound:9068529|FD-Score:-3.21|P-value:1.20E-4 ID:SGTC_3210|Compound:9128157|FD-Score:-3.09|P-value:2.00E-4 ID:SGTC_3214|Compound:9129694|FD-Score:4.67|P-value:5.94E-8 ID:SGTC_3215|Compound:9129942|FD-Score:3.28|P-value:1.01E-4 ID:SGTC_3340|Compound:9146634|FD-Score:2.83|P-value:6.88E-4

Top fitness defect scores for YML130C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature		Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_1239 0337-0584 36.90 35.4 µM 0 unfolded protein response 1 3.82E-298 21.40
2 SGTC_16 erodoxin 30.90 22.8 µM 1.38E-270 unfolded protein response 1 1.50E-209 15.70
3 SGTC_1918 oxolan-2-ylmethyl 3-bromobenzoate 7.40 70.1 µM 2.03E-17 1 6.60E-14 2.92
4 SGTC_513 4245-1575 5.42 41.7 µM 3.89E-10 unfolded protein response 13 3.02E-8 0.02
5 SGTC_3214 9129694 4.67 49.5 µM 5.94E-8 4 1.53E-6 0.43
6 SGTC_1184 1269-1718 4.37 139.0 µM 3.48E-7 2 6.08E-6 0.43
7 SGTC_1908 st060353 3.46 56.5 µM 4.48E-5 RPP1 & pyrimidine depletion 17 2.73E-4 0.06
8 SGTC_3215 9129942 3.28 49.5 µM 1.01E-4 12 5.20E-4 0.01
9 SGTC_2794 7785382 3.17 27.3 µM 1.63E-4 26 7.57E-4 0.04
10 SGTC_1558 st069300 3.17 45.6 µM 1.67E-4 6 7.70E-4 0.02
11 SGTC_1917 st066507 3.13 28.5 µM 1.96E-4 Golgi 19 8.75E-4 0.02
12 SGTC_1130 3454-2858 3.07 15.9 µM 2.50E-4 NEO1 23 0.00106 0.06
13 SGTC_272 doxorubicin 2.97 8.1 µM 3.78E-4 anthracycline transcription coupled DNA repair 20 0.00147 0.01
14 SGTC_2261 9010538 2.97 184.7 µM 3.80E-4 14 0.00147 0.04
15 SGTC_405 ebelactone b 2.94 50.0 µM 4.43E-4 4 0.00166 0.03
16 SGTC_1008 4106-0037 2.91 6.0 µM 4.86E-4 PDR1 14 0.00179 0.06
17 SGTC_356 1634-0012 2.91 32.5 µM 4.94E-4 15 0.00181 0.03
18 SGTC_2216 7189485 2.91 200.0 µM 4.99E-4 8 0.00183 0.01
19 SGTC_2566 crystal violet carbinol 2.86 226.0 nM 6.02E-4 45 0.00212 0.02
20 SGTC_1909 st060837 2.83 51.5 µM 6.71E-4 20 0.00231 0.00

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.614 0 YFL032W_d YFL032W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene HAC1/YFL031W; YFL032W is not an essential gene
0.530 4.28E-242 YDL045C FAD1 het Flavin adenine dinucleotide (FAD) synthetase, performs the second step in synthesis of FAD from riboflavin
0.485 2.19E-197 YPL244C HUT1 hom Protein with a role in UDP-galactose transport to the Golgi lumen, has similarity to human UDP-galactose transporter UGTrel1, exhibits a genetic interaction with S. cerevisiae ERO1
0.474 5.58E-188 YHR079C IRE1 hom Serine-threonine kinase and endoribonuclease; transmembrane protein that mediates the unfolded protein response (UPR) by regulating Hac1p synthesis through HAC1 mRNA splicing; Kar2p binds inactive Ire1p and releases from it upon ER stress
0.467 3.13E-181 YFL031W HAC1 hom Basic leucine zipper (bZIP) transcription factor (ATF/CREB1 homolog); regulates the unfolded protein response, via UPRE binding, and membrane biogenesis; ER stress-induced splicing pathway facilitates efficient Hac1p synthesis; protein abundance increases in response to DNA replication stress
0.417 4.00E-141 YMR161W HLJ1 hom Co-chaperone for Hsp40p, anchored in the ER membrane; with its homolog Ydj1p promotes ER-associated protein degradation (ERAD) of integral membrane substrates; similar to E. coli DnaJ
0.333 7.73E-88 YLR292C SEC72 hom Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER
0.234 3.74E-43 YPR074C TKL1 hom Transketolase; catalyzes conversion of xylulose-5-phosphate and ribose-5-phosphate to sedoheptulose-7-phosphate and glyceraldehyde-3-phosphate in the pentose phosphate pathway; needed for synthesis of aromatic amino acids; TKL1 has a paralog, TKL2, that arose from the whole genome duplication
0.232 2.11E-42 YLL062C MHT1 hom S-methylmethionine-homocysteine methyltransferase, functions along with Sam4p in the conversion of S-adenosylmethionine (AdoMet) to methionine to control the methionine/AdoMet ratio
0.224 1.67E-39 YPL243W SRP68 het Core component of the signal recognition particle (SRP) complex; SRP complex functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress
0.202 2.70E-32 YLR132C USB1 het Putative phosphodiesterase specific for U6 snRNA 3' end modification; trims the 3' poly(u) tract to leave a terminal 3' phosphate; human homolog, hUSB1 (aka C16orf57) produces a 2',3' cyclic phosphate; mutations in hUSB1 are associated with a rare skin condition (OMIM 604173); essential protein that localizes to the nucleus and mitochondria; overexpression suppresses the respiratory defects of oxa1 and mtf2 mutants
0.192 3.74E-29 YCL051W LRE1 hom Protein involved in control of cell wall structure and stress response; overproduction confers resistance to cell-wall degrading enzymes; exhibits genetic interactions with genes involved in the cell wall integrity pathway;
LRE1 has a paralog, HLR1, that arose from the whole genome duplication
0.188 4.61E-28 YCL052C PBN1 het Essential component of glycosylphosphatidylinositol-mannosyltransferase I, required for the autocatalytic post-translational processing of the protease B precursor Prb1p, localizes to ER in lumenal orientation; homolog of mammalian PIG-X
0.186 1.63E-27 YHL020C OPI1 hom Transcriptional regulator of a variety of genes; phosphorylation by protein kinase A stimulates Opi1p function in negative regulation of phospholipid biosynthetic genes; involved in telomere maintenance
0.186 2.20E-27 YIL167W SDL1 hom Open reading frame, unlikely to produce a functional protein in S288C; in closely related species and other S. cerevisiae strain backgrounds YIL167W and adjacent ORF, YIL168W, constitute a single ORF encoding L-serine dehydratase