YMR025W / CSI1

Subunit of the Cop9 signalosome, which is required for deneddylation, or removal of the ubiquitin-like protein Rub1p from Cdc53p (cullin); involved in adaptation to pheromone signaling; functional equivalent of canonical Csn6 subunit of the COP9 signalosome

Zygosity: Homozygous strain
fixedexpanded
Profile for YMR025W / CSI1

Click on Significant Values for Screen Details ID:SGTC_127|Compound:0180-0333|FD-Score:3.02|P-value:8.53E-4 ID:SGTC_395|Compound:anisomycin|FD-Score:3.33|P-value:2.68E-4 ID:SGTC_409|Compound:arachidonic acid|FD-Score:-3.34|P-value:3.14E-4 ID:SGTC_588|Compound:3970-1144|FD-Score:3.23|P-value:3.99E-4 ID:SGTC_899|Compound:1083-0020|FD-Score:3.09|P-value:6.67E-4 ID:SGTC_990|Compound:3985-0011|FD-Score:4.09|P-value:1.13E-5 ID:SGTC_1046|Compound:2001-0062|FD-Score:-3.08|P-value:8.09E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.46|P-value:2.02E-4 ID:SGTC_1192|Compound:1488-0095|FD-Score:3.07|P-value:7.02E-4 ID:SGTC_1216|Compound:1165-0504|FD-Score:3.41|P-value:1.99E-4 ID:SGTC_1482|Compound:3346-2049|FD-Score:3.14|P-value:5.55E-4 ID:SGTC_1544|Compound:7-methoxy-4-methylcoumarin|FD-Score:3.24|P-value:3.79E-4 ID:SGTC_1569|Compound:carminic acid|FD-Score:-3.51|P-value:1.60E-4 ID:SGTC_1576|Compound:erythromycin ethylsuccinate|FD-Score:2.98|P-value:9.82E-4 ID:SGTC_1885|Compound:st057546|FD-Score:4.56|P-value:1.13E-6 ID:SGTC_1886|Compound:st057356|FD-Score:3.76|P-value:4.71E-5 ID:SGTC_1903|Compound:st060211|FD-Score:4.06|P-value:1.29E-5 ID:SGTC_1904|Compound:st060225|FD-Score:-3.18|P-value:5.61E-4 ID:SGTC_1971|Compound:st069429|FD-Score:3.35|P-value:2.53E-4 ID:SGTC_1986|Compound:st071812|FD-Score:-3.19|P-value:5.54E-4 ID:SGTC_1997|Compound:st073024|FD-Score:3.05|P-value:7.74E-4 ID:SGTC_2262|Compound:9014885|FD-Score:4.45|P-value:1.97E-6 ID:SGTC_2573|Compound:2',4'-dihydroxychalcone|FD-Score:3.19|P-value:4.60E-4 ID:SGTC_2602|Compound:kawain|FD-Score:3.01|P-value:8.78E-4 ID:SGTC_2700|Compound:st077718|FD-Score:3.30|P-value:3.08E-4 ID:SGTC_2708|Compound:st078559|FD-Score:3.09|P-value:6.62E-4 ID:SGTC_2711|Compound:st079103|FD-Score:3.52|P-value:1.31E-4 ID:SGTC_2716|Compound:st080858|FD-Score:4.95|P-value:1.43E-7 ID:SGTC_2839|Compound:9003951|FD-Score:3.68|P-value:6.75E-5 ID:SGTC_2847|Compound:9005661|FD-Score:3.98|P-value:1.80E-5 ID:SGTC_2935|Compound:9038016|FD-Score:3.31|P-value:2.96E-4 ID:SGTC_3130|Compound:9127414|FD-Score:-3.45|P-value:2.03E-4 ID:SGTC_127|Compound:0180-0333|FD-Score:3.02|P-value:8.53E-4 ID:SGTC_395|Compound:anisomycin|FD-Score:3.33|P-value:2.68E-4 ID:SGTC_409|Compound:arachidonic acid|FD-Score:-3.34|P-value:3.14E-4 ID:SGTC_588|Compound:3970-1144|FD-Score:3.23|P-value:3.99E-4 ID:SGTC_899|Compound:1083-0020|FD-Score:3.09|P-value:6.67E-4 ID:SGTC_990|Compound:3985-0011|FD-Score:4.09|P-value:1.13E-5 ID:SGTC_1046|Compound:2001-0062|FD-Score:-3.08|P-value:8.09E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.46|P-value:2.02E-4 ID:SGTC_1192|Compound:1488-0095|FD-Score:3.07|P-value:7.02E-4 ID:SGTC_1216|Compound:1165-0504|FD-Score:3.41|P-value:1.99E-4 ID:SGTC_1482|Compound:3346-2049|FD-Score:3.14|P-value:5.55E-4 ID:SGTC_1544|Compound:7-methoxy-4-methylcoumarin|FD-Score:3.24|P-value:3.79E-4 ID:SGTC_1569|Compound:carminic acid|FD-Score:-3.51|P-value:1.60E-4 ID:SGTC_1576|Compound:erythromycin ethylsuccinate|FD-Score:2.98|P-value:9.82E-4 ID:SGTC_1885|Compound:st057546|FD-Score:4.56|P-value:1.13E-6 ID:SGTC_1886|Compound:st057356|FD-Score:3.76|P-value:4.71E-5 ID:SGTC_1903|Compound:st060211|FD-Score:4.06|P-value:1.29E-5 ID:SGTC_1904|Compound:st060225|FD-Score:-3.18|P-value:5.61E-4 ID:SGTC_1971|Compound:st069429|FD-Score:3.35|P-value:2.53E-4 ID:SGTC_1986|Compound:st071812|FD-Score:-3.19|P-value:5.54E-4 ID:SGTC_1997|Compound:st073024|FD-Score:3.05|P-value:7.74E-4 ID:SGTC_2262|Compound:9014885|FD-Score:4.45|P-value:1.97E-6 ID:SGTC_2573|Compound:2',4'-dihydroxychalcone|FD-Score:3.19|P-value:4.60E-4 ID:SGTC_2602|Compound:kawain|FD-Score:3.01|P-value:8.78E-4 ID:SGTC_2700|Compound:st077718|FD-Score:3.30|P-value:3.08E-4 ID:SGTC_2708|Compound:st078559|FD-Score:3.09|P-value:6.62E-4 ID:SGTC_2711|Compound:st079103|FD-Score:3.52|P-value:1.31E-4 ID:SGTC_2716|Compound:st080858|FD-Score:4.95|P-value:1.43E-7 ID:SGTC_2839|Compound:9003951|FD-Score:3.68|P-value:6.75E-5 ID:SGTC_2847|Compound:9005661|FD-Score:3.98|P-value:1.80E-5 ID:SGTC_2935|Compound:9038016|FD-Score:3.31|P-value:2.96E-4 ID:SGTC_3130|Compound:9127414|FD-Score:-3.45|P-value:2.03E-4

Top fitness defect scores for YMR025W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2716 st080858 4.95 38.6 µM 1.43E-7 60S ribosome export 6 3.64E-7
2 SGTC_1885 st057546 4.56 99.9 µM 1.13E-6 10 2.52E-6
3 SGTC_2262 9014885 4.45 67.5 µM 1.97E-6 3 4.25E-6
4 SGTC_990 3985-0011 4.09 41.3 µM 1.13E-5 amide catabolism 17 2.19E-5
5 SGTC_1903 st060211 4.06 78.3 µM 1.29E-5 25 2.49E-5
6 SGTC_2847 9005661 3.98 71.4 µM 1.80E-5 8 3.40E-5
7 SGTC_1886 st057356 3.76 49.9 µM 4.71E-5 60S ribosome export 23 8.36E-5
8 SGTC_2839 9003951 3.68 26.0 µM 6.75E-5 14 1.17E-4
9 SGTC_2711 st079103 3.52 59.1 µM 1.31E-4 13 2.18E-4
10 SGTC_1216 1165-0504 3.41 9.7 µM 1.99E-4 27 3.24E-4
11 SGTC_1971 st069429 3.35 63.5 µM 2.53E-4 60S ribosome export 29 4.06E-4
12 SGTC_395 anisomycin 3.33 6.2 µM 2.68E-4 20 4.28E-4
13 SGTC_2935 9038016 3.31 30.0 µM 2.96E-4 41 4.69E-4
14 SGTC_2700 st077718 3.30 96.8 µM 3.08E-4 28 4.88E-4
15 SGTC_1544 7-methoxy-4-methylcoumarin 3.24 105.0 µM 3.79E-4 43 5.93E-4
16 SGTC_588 3970-1144 3.23 52.9 µM 3.99E-4 51 6.22E-4
17 SGTC_2573 2',4'-dihydroxychalcone 3.19 15.0 µM 4.60E-4 100 7.10E-4
18 SGTC_1482 3346-2049 3.14 8.5 µM 5.55E-4 55 8.47E-4
19 SGTC_2708 st078559 3.09 56.0 µM 6.62E-4 26 0.00100
20 SGTC_899 1083-0020 3.09 478.0 µM 6.67E-4 53 0.00101

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.172 1.26E-23 YMR129W POM152 hom Glycoprotein subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication; homologous to human NUP210
0.162 4.30E-21 YLR023C IZH3 hom Membrane protein involved in zinc ion homeostasis, member of the four-protein IZH family, expression induced by zinc deficiency; deletion reduces sensitivity to elevated zinc and shortens lag phase, overexpression reduces Zap1p activity
0.144 5.91E-17 YGL017W ATE1 hom Arginyl-tRNA-protein transferase, catalyzes post-translational conjugation of arginine to the amino termini of acceptor proteins which are then subject to degradation via the N-end rule pathway
0.126 2.49E-13 YBL036C YBL036C hom Putative non-specific single-domain racemase based on structural similarity; binds pyridoxal 5'-phosphate; expression of GFP-fusion protein induced in response to the DNA-damaging agent MMS
0.112 7.64E-11 YEL025C_p YEL025C_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus
0.109 2.59E-10 YBR258C SHG1 hom Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres
0.097 1.89E-8 YMR104C YPK2 hom Protein kinase with similarity to serine/threonine protein kinase Ypk1p; functionally redundant with YPK1 at the genetic level; participates in a signaling pathway required for optimal cell wall integrity; homolog of mammalian kinase SGK; YPK2 has a paralog, YPK1, that arose from the whole genome duplication
0.095 3.69E-8 YOR298C-A MBF1 hom Transcriptional coactivator; bridges the DNA-binding region of Gcn4p and TATA-binding protein Spt15p; suppressor of frameshift mutations; protein abundance increases in response to DNA replication stress
0.093 6.56E-8 YDR286C_p YDR286C_p hom Putative protein of unknown function; predicted to have thiol-disulfide oxidoreductase active site
0.093 7.56E-8 YPR010C RPA135 het RNA polymerase I second largest subunit A135
0.092 8.41E-8 YML107C PML39 hom Protein required for nuclear retention of unspliced pre-mRNAs along with Mlp1p and Pml1p; anchored to nuclear pore complex via Mlp1p and Mlp2p; found with the subset of nuclear pores farthest from the nucleolus; may interact with ribosomes
0.092 1.03E-7 YJR106W ECM27 hom Putative protein of unknown function; may play a role in cell wall biosynthesis, mutants are hypersensitive to Papulacandin B; null mutants have increased plasmid loss; displays a two-hybrid interaction with Pdr5p
0.090 1.84E-7 YGR149W_p YGR149W_p hom Putative protein of unknown function; predicted to be an integal membrane protein
0.089 2.19E-7 YKL163W PIR3 hom O-glycosylated covalently-bound cell wall protein; required for cell wall stability; expression is cell cycle regulated, peaking in M/G1 and also subject to regulation by the cell integrity pathway; PIR3 has a paralog, HSP150, that arose from the whole genome duplication
0.089 2.32E-7 YLL012W YEH1 hom Steryl ester hydrolase, one of three gene products (Yeh1p, Yeh2p, Tgl1p) responsible for steryl ester hydrolase activity and involved in sterol homeostasis; localized to lipid particle membranes