YMR065W / KAR5

Protein required for nuclear membrane fusion during karyogamy, localizes to the membrane with a soluble portion in the endoplasmic reticulum lumen, may form a complex with Jem1p and Kar2p; expression of the gene is regulated by pheromone

Zygosity: Homozygous strain
fixedexpanded
Profile for YMR065W / KAR5

Click on Significant Values for Screen Details ID:SGTC_1|Compound:0258-0098|FD-Score:3.53|P-value:1.91E-4 ID:SGTC_108|Compound:0509-0257|FD-Score:-3.31|P-value:4.44E-4 ID:SGTC_109|Compound:0132-0036|FD-Score:-3.18|P-value:6.83E-4 ID:SGTC_117|Compound:lucigenin|FD-Score:3.33|P-value:3.98E-4 ID:SGTC_125|Compound:0133-0007|FD-Score:3.18|P-value:6.92E-4 ID:SGTC_256|Compound:clotrimazole|FD-Score:3.77|P-value:7.33E-5 ID:SGTC_314|Compound:1671-0008|FD-Score:5.65|P-value:6.65E-9 ID:SGTC_476|Compound:mead acid|FD-Score:3.42|P-value:2.92E-4 ID:SGTC_599|Compound:1935-0052|FD-Score:4.62|P-value:1.71E-6 ID:SGTC_804|Compound:1595-0054|FD-Score:3.67|P-value:1.11E-4 ID:SGTC_967|Compound:1306-0166|FD-Score:3.35|P-value:3.80E-4 ID:SGTC_1045|Compound:1348-1485|FD-Score:3.10|P-value:9.15E-4 ID:SGTC_1097|Compound:itavastatin|FD-Score:4.19|P-value:1.27E-5 ID:SGTC_1131|Compound:3454-3128|FD-Score:4.29|P-value:8.03E-6 ID:SGTC_1152|Compound:3909-8331|FD-Score:3.81|P-value:6.36E-5 ID:SGTC_1163|Compound:k060-0048|FD-Score:3.17|P-value:7.21E-4 ID:SGTC_1253|Compound:0550-0034|FD-Score:3.13|P-value:8.04E-4 ID:SGTC_1405|Compound:3909-7894|FD-Score:3.74|P-value:8.42E-5 ID:SGTC_1424|Compound:4048-1941|FD-Score:3.21|P-value:6.15E-4 ID:SGTC_1593|Compound:justicidin b . 9-(1,3-benzodioxol-5-yl)- 6,7-dimethoxynaphtho[2,3-c]furan-1(3h)-one|FD-Score:3.65|P-value:1.20E-4 ID:SGTC_1598|Compound:3(2'-chlorophenyl)-7-hydroxy-4-phenylcoumarin|FD-Score:3.08|P-value:9.79E-4 ID:SGTC_1665|Compound:st014639|FD-Score:3.73|P-value:8.58E-5 ID:SGTC_1675|Compound:st018485|FD-Score:4.30|P-value:7.64E-6 ID:SGTC_1861|Compound:st057647|FD-Score:3.67|P-value:1.10E-4 ID:SGTC_1893|Compound:st059353|FD-Score:3.17|P-value:7.09E-4 ID:SGTC_1906|Compound:st060206|FD-Score:4.00|P-value:2.83E-5 ID:SGTC_1967|Compound:st070177|FD-Score:5.55|P-value:1.16E-8 ID:SGTC_2005|Compound:4024620|FD-Score:3.32|P-value:4.11E-4 ID:SGTC_2086|Compound:5274770|FD-Score:3.27|P-value:5.04E-4 ID:SGTC_2088|Compound:5278280|FD-Score:-3.17|P-value:7.17E-4 ID:SGTC_2155|Compound:5570423|FD-Score:3.19|P-value:6.66E-4 ID:SGTC_2322|Compound:7413319|FD-Score:4.12|P-value:1.73E-5 ID:SGTC_2359|Compound:9039622|FD-Score:3.17|P-value:7.04E-4 ID:SGTC_2563|Compound:tomatine|FD-Score:3.56|P-value:1.70E-4 ID:SGTC_2581|Compound:avocatin a|FD-Score:-3.97|P-value:3.20E-5 ID:SGTC_2589|Compound:lobaric acid|FD-Score:-4.43|P-value:4.09E-6 ID:SGTC_2705|Compound:st078069|FD-Score:3.19|P-value:6.63E-4 ID:SGTC_2710|Compound:st078562|FD-Score:4.35|P-value:6.02E-6 ID:SGTC_2864|Compound:9034755|FD-Score:-3.98|P-value:3.13E-5 ID:SGTC_2877|Compound:9040724|FD-Score:4.45|P-value:3.69E-6 ID:SGTC_2917|Compound:7979103|FD-Score:3.14|P-value:7.84E-4 ID:SGTC_2963|Compound:9082507|FD-Score:5.84|P-value:2.14E-9 ID:SGTC_2964|Compound:9083345|FD-Score:3.76|P-value:7.66E-5 ID:SGTC_2994|Compound:9053520|FD-Score:3.70|P-value:9.67E-5 ID:SGTC_3045|Compound:9092950|FD-Score:6.57|P-value:1.96E-11 ID:SGTC_3047|Compound:9092122|FD-Score:4.19|P-value:1.22E-5 ID:SGTC_3103|Compound:9120822|FD-Score:-4.17|P-value:1.39E-5 ID:SGTC_3118|Compound:9124250|FD-Score:3.48|P-value:2.27E-4 ID:SGTC_3354|Compound:9151612|FD-Score:3.23|P-value:5.71E-4 ID:SGTC_1|Compound:0258-0098|FD-Score:3.53|P-value:1.91E-4 ID:SGTC_108|Compound:0509-0257|FD-Score:-3.31|P-value:4.44E-4 ID:SGTC_109|Compound:0132-0036|FD-Score:-3.18|P-value:6.83E-4 ID:SGTC_117|Compound:lucigenin|FD-Score:3.33|P-value:3.98E-4 ID:SGTC_125|Compound:0133-0007|FD-Score:3.18|P-value:6.92E-4 ID:SGTC_256|Compound:clotrimazole|FD-Score:3.77|P-value:7.33E-5 ID:SGTC_314|Compound:1671-0008|FD-Score:5.65|P-value:6.65E-9 ID:SGTC_476|Compound:mead acid|FD-Score:3.42|P-value:2.92E-4 ID:SGTC_599|Compound:1935-0052|FD-Score:4.62|P-value:1.71E-6 ID:SGTC_804|Compound:1595-0054|FD-Score:3.67|P-value:1.11E-4 ID:SGTC_967|Compound:1306-0166|FD-Score:3.35|P-value:3.80E-4 ID:SGTC_1045|Compound:1348-1485|FD-Score:3.10|P-value:9.15E-4 ID:SGTC_1097|Compound:itavastatin|FD-Score:4.19|P-value:1.27E-5 ID:SGTC_1131|Compound:3454-3128|FD-Score:4.29|P-value:8.03E-6 ID:SGTC_1152|Compound:3909-8331|FD-Score:3.81|P-value:6.36E-5 ID:SGTC_1163|Compound:k060-0048|FD-Score:3.17|P-value:7.21E-4 ID:SGTC_1253|Compound:0550-0034|FD-Score:3.13|P-value:8.04E-4 ID:SGTC_1405|Compound:3909-7894|FD-Score:3.74|P-value:8.42E-5 ID:SGTC_1424|Compound:4048-1941|FD-Score:3.21|P-value:6.15E-4 ID:SGTC_1593|Compound:justicidin b . 9-(1,3-benzodioxol-5-yl)- 6,7-dimethoxynaphtho[2,3-c]furan-1(3h)-one|FD-Score:3.65|P-value:1.20E-4 ID:SGTC_1598|Compound:3(2'-chlorophenyl)-7-hydroxy-4-phenylcoumarin|FD-Score:3.08|P-value:9.79E-4 ID:SGTC_1665|Compound:st014639|FD-Score:3.73|P-value:8.58E-5 ID:SGTC_1675|Compound:st018485|FD-Score:4.30|P-value:7.64E-6 ID:SGTC_1861|Compound:st057647|FD-Score:3.67|P-value:1.10E-4 ID:SGTC_1893|Compound:st059353|FD-Score:3.17|P-value:7.09E-4 ID:SGTC_1906|Compound:st060206|FD-Score:4.00|P-value:2.83E-5 ID:SGTC_1967|Compound:st070177|FD-Score:5.55|P-value:1.16E-8 ID:SGTC_2005|Compound:4024620|FD-Score:3.32|P-value:4.11E-4 ID:SGTC_2086|Compound:5274770|FD-Score:3.27|P-value:5.04E-4 ID:SGTC_2088|Compound:5278280|FD-Score:-3.17|P-value:7.17E-4 ID:SGTC_2155|Compound:5570423|FD-Score:3.19|P-value:6.66E-4 ID:SGTC_2322|Compound:7413319|FD-Score:4.12|P-value:1.73E-5 ID:SGTC_2359|Compound:9039622|FD-Score:3.17|P-value:7.04E-4 ID:SGTC_2563|Compound:tomatine|FD-Score:3.56|P-value:1.70E-4 ID:SGTC_2581|Compound:avocatin a|FD-Score:-3.97|P-value:3.20E-5 ID:SGTC_2589|Compound:lobaric acid|FD-Score:-4.43|P-value:4.09E-6 ID:SGTC_2705|Compound:st078069|FD-Score:3.19|P-value:6.63E-4 ID:SGTC_2710|Compound:st078562|FD-Score:4.35|P-value:6.02E-6 ID:SGTC_2864|Compound:9034755|FD-Score:-3.98|P-value:3.13E-5 ID:SGTC_2877|Compound:9040724|FD-Score:4.45|P-value:3.69E-6 ID:SGTC_2917|Compound:7979103|FD-Score:3.14|P-value:7.84E-4 ID:SGTC_2963|Compound:9082507|FD-Score:5.84|P-value:2.14E-9 ID:SGTC_2964|Compound:9083345|FD-Score:3.76|P-value:7.66E-5 ID:SGTC_2994|Compound:9053520|FD-Score:3.70|P-value:9.67E-5 ID:SGTC_3045|Compound:9092950|FD-Score:6.57|P-value:1.96E-11 ID:SGTC_3047|Compound:9092122|FD-Score:4.19|P-value:1.22E-5 ID:SGTC_3103|Compound:9120822|FD-Score:-4.17|P-value:1.39E-5 ID:SGTC_3118|Compound:9124250|FD-Score:3.48|P-value:2.27E-4 ID:SGTC_3354|Compound:9151612|FD-Score:3.23|P-value:5.71E-4

Top fitness defect scores for YMR065W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_3045 9092950 6.57 49.5 µM 1.96E-11 1 2.57E-11
2 SGTC_2963 9082507 5.84 9.0 µM 2.14E-9 6 2.65E-9
3 SGTC_314 1671-0008 5.65 2.7 µM 6.65E-9 10 8.14E-9
4 SGTC_1967 st070177 5.55 51.4 µM 1.16E-8 2 1.41E-8
5 SGTC_599 1935-0052 4.62 86.5 µM 1.71E-6 7 1.96E-6
6 SGTC_2877 9040724 4.45 71.4 µM 3.69E-6 4 4.20E-6
7 SGTC_2710 st078562 4.35 53.7 µM 6.02E-6 9 6.81E-6
8 SGTC_1675 st018485 4.30 24.5 µM 7.64E-6 60S ribosome export 18 8.61E-6
9 SGTC_1131 3454-3128 4.29 32.3 µM 8.03E-6 30 9.06E-6
10 SGTC_3047 9092122 4.19 49.5 µM 1.22E-5 14 1.37E-5
11 SGTC_1097 itavastatin 4.19 7.2 µM 1.27E-5 azole & statin 16 1.42E-5
12 SGTC_2322 7413319 4.12 38.2 µM 1.73E-5 ERAD & cell cycle 144 1.93E-5
13 SGTC_1906 st060206 4.00 94.7 µM 2.83E-5 19 3.14E-5
14 SGTC_1152 3909-8331 3.81 15.9 µM 6.36E-5 9 7.00E-5
15 SGTC_256 clotrimazole 3.77 625.0 nM 7.33E-5 ergosterol depletion effects on membrane 33 8.05E-5
16 SGTC_2964 9083345 3.76 53.4 µM 7.66E-5 23 8.42E-5
17 SGTC_1405 3909-7894 3.74 12.0 µM 8.42E-5 60S ribosome export 15 9.24E-5
18 SGTC_1665 st014639 3.73 11.4 µM 8.58E-5 NEO1 27 9.42E-5
19 SGTC_2994 9053520 3.70 71.4 µM 9.67E-5 RPP1 & pyrimidine depletion 29 1.06E-4
20 SGTC_1861 st057647 3.67 17.4 µM 1.10E-4 28 1.20E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.106 6.21E-10 YMR107W SPG4 hom Protein required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources
0.087 4.83E-7 YGR043C NQM1 hom Transaldolase of unknown function; transcription is repressed by Mot1p and induced by alpha-factor and during diauxic shift
0.084 1.07E-6 YOR356W CIR2 hom Putative ortholog of human electron transfer flavoprotein dehydrogenase (ETF-dH); found in a large supramolecular complex with other mitochondrial dehydrogenases; may have a role in oxidative stress response
0.082 1.96E-6 YMR226C YMR226C hom NADP(+)-dependent dehydrogenase; acts on serine, L-allo-threonine, and other 3-hydroxy acids; green fluorescent protein fusion protein localizes to the cytoplasm and nucleus; may interact with ribosomes, based on co-purification experiments
0.081 2.52E-6 YOL136C PFK27 hom 6-phosphofructo-2-kinase, catalyzes synthesis of fructose-2,6-bisphosphate; inhibited by phosphoenolpyruvate and sn-glycerol 3-phosphate, expression induced by glucose and sucrose, transcriptional regulation involves protein kinase A
0.080 3.66E-6 YOR360C PDE2 hom High-affinity cyclic AMP phosphodiesterase, component of the cAMP-dependent protein kinase signaling system, protects the cell from extracellular cAMP, contains readthrough motif surrounding termination codon
0.078 6.37E-6 YMR175W SIP18 hom Phospholipid-binding hydrophilin; essential to overcome desiccation-rehydration process; expression is induced by osmotic stress
0.076 9.20E-6 YGL171W ROK1 het RNA-dependent ATPase; involved in pre-rRNA processing at sites A0, A1, and A2, and in control of cell cycle progression; contains two upstream open reading frames (uORFs) in 5' untranslated region which regulate translation
0.076 9.74E-6 YOL126C MDH2 hom Cytoplasmic malate dehydrogenase, one of three isozymes that catalyze interconversion of malate and oxaloacetate; involved in the glyoxylate cycle and gluconeogenesis during growth on two-carbon compounds; interacts with Pck1p and Fbp1
0.076 1.11E-5 YLR064W PER33 hom Protein that localizes to the endoplasmic reticulum; also associates with the nuclear pore complex; deletion extends chronological lifespan; highly conserved across species, orthologous to human TMEM33 and paralogous to Pom33p; protein abundance increases in response to DNA replication stress
0.074 1.78E-5 YCL025C AGP1 hom Low-affinity amino acid permease with broad substrate range; involved in uptake of asparagine, glutamine, and other amino acids; expression regulated by SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p); AGP1 has a paralog, GNP1, that arose from the whole genome duplication
0.072 2.81E-5 YOL052C SPE2 hom S-adenosylmethionine decarboxylase, required for the biosynthesis of spermidine and spermine; cells lacking Spe2p require spermine or spermidine for growth in the presence of oxygen but not when grown anaerobically
0.072 2.92E-5 YDR005C MAF1 hom Highly conserved negative regulator of RNA polymerase III; binds to the N-terminal domain of the Rpc160p subunit of Pol III to prevent closed-complex formation; localization and activity are regulated by phosphorylation, mediated by TORC1, protein kinase A, and Sch9p; localizes to cytoplasm during vegetative growth and translocates to the nucleus and nucleolus under stress conditions
0.072 3.20E-5 YBR085W AAC3 hom Mitochondrial inner membrane ADP/ATP translocator; exchanges cytosolic ADP for mitochondrially synthesized ATP; expressed under anaerobic conditions; similar to Aac1p; has roles in maintenance of viability and in respiration; AAC3 has a paralog, PET9, that arose from the whole genome duplication
0.072 3.28E-5 YLL059C_d YLL059C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data