YMR080C / NAM7

ATP-dependent RNA helicase of the SFI superfamily; involved in nonsense mediated mRNA decay; required for efficient translation termination at nonsense codons and targeting of NMD substrates to P-bodies; involved in telomere maintenance; forms cytoplasmic foci upon DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YMR080C / NAM7

Click on Significant Values for Screen Details ID:SGTC_6|Compound:0844-0013|FD-Score:3.62|P-value:3.88E-4 ID:SGTC_71|Compound:0417-1750|FD-Score:4.25|P-value:3.95E-5 ID:SGTC_76|Compound:0433-0009|FD-Score:4.77|P-value:4.75E-6 ID:SGTC_249|Compound:wiskostatin|FD-Score:-3.87|P-value:1.56E-4 ID:SGTC_374|Compound:0880-0231|FD-Score:3.59|P-value:4.32E-4 ID:SGTC_909|Compound:3448-9333|FD-Score:4.24|P-value:4.04E-5 ID:SGTC_1046|Compound:2001-0062|FD-Score:4.01|P-value:9.81E-5 ID:SGTC_1065|Compound:prochlorperazine maleate|FD-Score:3.92|P-value:1.37E-4 ID:SGTC_1201|Compound:1889-3372|FD-Score:-3.94|P-value:1.17E-4 ID:SGTC_1628|Compound:st004252|FD-Score:5.01|P-value:1.59E-6 ID:SGTC_1730|Compound:st033808|FD-Score:-3.44|P-value:6.54E-4 ID:SGTC_2072|Compound:5231554|FD-Score:3.65|P-value:3.49E-4 ID:SGTC_2079|Compound:5212404|FD-Score:3.41|P-value:7.68E-4 ID:SGTC_2081|Compound:5214970|FD-Score:4.21|P-value:4.53E-5 ID:SGTC_2176|Compound:5809774|FD-Score:-3.37|P-value:8.20E-4 ID:SGTC_2237|Compound:6667747|FD-Score:-3.42|P-value:7.00E-4 ID:SGTC_2309|Compound:7664088|FD-Score:-4.25|P-value:3.72E-5 ID:SGTC_2410|Compound:aloe-emodin-8-o-glycoside|FD-Score:-3.40|P-value:7.65E-4 ID:SGTC_2430|Compound:5571206|FD-Score:-5.44|P-value:1.95E-7 ID:SGTC_2433|Compound:5607874|FD-Score:-4.14|P-value:5.57E-5 ID:SGTC_2435|Compound:5706476|FD-Score:-3.82|P-value:1.81E-4 ID:SGTC_2450|Compound:5739737|FD-Score:3.35|P-value:9.23E-4 ID:SGTC_2494|Compound:18-aminoabieta-8,11,13-triene sulfate|FD-Score:5.05|P-value:1.35E-6 ID:SGTC_2576|Compound:guaiol|FD-Score:-3.82|P-value:1.81E-4 ID:SGTC_2625|Compound:euphol|FD-Score:-3.78|P-value:2.09E-4 ID:SGTC_2692|Compound:st077426|FD-Score:-3.34|P-value:9.14E-4 ID:SGTC_2695|Compound:st077686|FD-Score:3.92|P-value:1.35E-4 ID:SGTC_2762|Compound:leflunomide|FD-Score:3.56|P-value:4.74E-4 ID:SGTC_6|Compound:0844-0013|FD-Score:3.62|P-value:3.88E-4 ID:SGTC_71|Compound:0417-1750|FD-Score:4.25|P-value:3.95E-5 ID:SGTC_76|Compound:0433-0009|FD-Score:4.77|P-value:4.75E-6 ID:SGTC_249|Compound:wiskostatin|FD-Score:-3.87|P-value:1.56E-4 ID:SGTC_374|Compound:0880-0231|FD-Score:3.59|P-value:4.32E-4 ID:SGTC_909|Compound:3448-9333|FD-Score:4.24|P-value:4.04E-5 ID:SGTC_1046|Compound:2001-0062|FD-Score:4.01|P-value:9.81E-5 ID:SGTC_1065|Compound:prochlorperazine maleate|FD-Score:3.92|P-value:1.37E-4 ID:SGTC_1201|Compound:1889-3372|FD-Score:-3.94|P-value:1.17E-4 ID:SGTC_1628|Compound:st004252|FD-Score:5.01|P-value:1.59E-6 ID:SGTC_1730|Compound:st033808|FD-Score:-3.44|P-value:6.54E-4 ID:SGTC_2072|Compound:5231554|FD-Score:3.65|P-value:3.49E-4 ID:SGTC_2079|Compound:5212404|FD-Score:3.41|P-value:7.68E-4 ID:SGTC_2081|Compound:5214970|FD-Score:4.21|P-value:4.53E-5 ID:SGTC_2176|Compound:5809774|FD-Score:-3.37|P-value:8.20E-4 ID:SGTC_2237|Compound:6667747|FD-Score:-3.42|P-value:7.00E-4 ID:SGTC_2309|Compound:7664088|FD-Score:-4.25|P-value:3.72E-5 ID:SGTC_2410|Compound:aloe-emodin-8-o-glycoside|FD-Score:-3.40|P-value:7.65E-4 ID:SGTC_2430|Compound:5571206|FD-Score:-5.44|P-value:1.95E-7 ID:SGTC_2433|Compound:5607874|FD-Score:-4.14|P-value:5.57E-5 ID:SGTC_2435|Compound:5706476|FD-Score:-3.82|P-value:1.81E-4 ID:SGTC_2450|Compound:5739737|FD-Score:3.35|P-value:9.23E-4 ID:SGTC_2494|Compound:18-aminoabieta-8,11,13-triene sulfate|FD-Score:5.05|P-value:1.35E-6 ID:SGTC_2576|Compound:guaiol|FD-Score:-3.82|P-value:1.81E-4 ID:SGTC_2625|Compound:euphol|FD-Score:-3.78|P-value:2.09E-4 ID:SGTC_2692|Compound:st077426|FD-Score:-3.34|P-value:9.14E-4 ID:SGTC_2695|Compound:st077686|FD-Score:3.92|P-value:1.35E-4 ID:SGTC_2762|Compound:leflunomide|FD-Score:3.56|P-value:4.74E-4

Top fitness defect scores for YMR080C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2494 18-aminoabieta-8,11,13-triene sulfate 5.05 3.7 µM 1.35E-6 32 2.21E-7
2 SGTC_1628 st004252 5.01 77.7 µM 1.59E-6 7 2.67E-7
3 SGTC_76 0433-0009 4.77 400.3 µM 4.75E-6 3 9.35E-7
4 SGTC_71 0417-1750 4.25 47.8 µM 3.95E-5 Golgi 35 1.07E-5
5 SGTC_909 3448-9333 4.24 149.0 µM 4.04E-5 15 1.10E-5
6 SGTC_2081 5214970 4.21 32.3 µM 4.53E-5 21 1.25E-5
7 SGTC_1046 2001-0062 4.01 216.0 µM 9.81E-5 16 3.04E-5
8 SGTC_2695 st077686 3.92 52.0 µM 1.35E-4 37 4.39E-5
9 SGTC_1065 prochlorperazine maleate 3.92 2.5 µM 1.37E-4 13 4.44E-5
10 SGTC_2072 5231554 3.65 129.0 µM 3.49E-4 13 1.30E-4
11 SGTC_6 0844-0013 3.62 19.8 µM 3.88E-4 124 1.47E-4
12 SGTC_374 0880-0231 3.59 82.3 µM 4.32E-4 iron homeostasis 31 1.67E-4
13 SGTC_2762 leflunomide 3.56 42.1 µM 4.74E-4 45 1.85E-4
14 SGTC_2079 5212404 3.41 121.0 µM 7.68E-4 22 3.22E-4
15 SGTC_2450 5739737 3.35 12.9 µM 9.23E-4 45 3.98E-4
16 SGTC_1904 st060225 3.32 78.0 µM 0.00103 38 4.50E-4
17 SGTC_2485 5633444 3.29 17.8 µM 0.00113 redox potentiating 72 5.02E-4
18 SGTC_1438 4092-1045 3.22 4.0 µM 0.00138 24 6.31E-4
19 SGTC_495 decylubiquinone 3.19 155.0 µM 0.00154 copper-dependent oxidative stress 63 7.13E-4
20 SGTC_3005 9076496 3.19 71.4 µM 0.00155 21 7.18E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.234 8.57E-43 YDR452W PPN1 hom Vacuolar endopolyphosphatase with a role in phosphate metabolism; functions as a homodimer; relocalizes from vacuole to cytoplasm upon DNA replication stress
0.193 1.84E-29 YPL004C LSP1 hom Primary component of eisosomes, which are large immobile patch structures at the cell cortex associated with endocytosis, along with Pil1p and Sur7p; null mutants show activation of Pkc1p/Ypk1p stress resistance pathways; member of the BAR domain family
0.153 4.64E-19 YBR176W ECM31 hom Ketopantoate hydroxymethyltransferase, required for pantothenic acid biosynthesis, converts 2-oxoisovalerate into 2-dehydropantoate
0.153 6.41E-19 YDR206W EBS1 hom Protein involved in inhibition of translation and nonsense-mediated decay; interacts with cap binding protein Cdc33p and with Nam7p; localizes to P-bodies upon glucose starvation; mRNA abundance regulated by mRNA decay factors
0.144 5.96E-17 YDR165W TRM82 hom Catalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p
0.144 6.61E-17 YMR191W SPG5 hom Protein required for proteasome assembly during quiescence; binds to base of the proteasome regulartory particle; required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources
0.140 3.18E-16 YOR214C_p YOR214C_p hom Putative protein of unknown function; YOR214C is not an essential gene
0.140 4.91E-16 YGL004C RPN14 hom Proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasome regulatory particle (RP); null mutants accumulate ubiquitinated Gcn4p and display decreased 26S proteasome stability; interacts with Rpt5p
0.139 5.63E-16 YKL215C OXP1 hom 5-oxoprolinase; enzyme is ATP-dependent and functions as a dimer; similar to mouse Oplah gene; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress
0.136 2.37E-15 YPR022C_p YPR022C_p hom Putative transcription factor, as suggested by computational analysis; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS
0.136 2.91E-15 YLR354C TAL1 hom Transaldolase, enzyme in the non-oxidative pentose phosphate pathway; converts sedoheptulose 7-phosphate and glyceraldehyde 3-phosphate to erythrose 4-phosphate and fructose 6-phosphate
0.136 3.07E-15 YHR021W-A_p ECM12_p hom Putative protein of unknown function; may contribute to cell wall biosynthesis, mutants display zymolyase hypersensitivity
0.132 1.68E-14 YNL334C SNO2 hom Protein of unknown function, nearly identical to Sno3p; expression is induced before the diauxic shift and also in the absence of thiamin
0.127 1.39E-13 YDR266C HEL2 hom RING finger ubiquitin ligase (E3); involved in ubiquitylation and degradation of excess histones; interacts with Ubc4p and Rad53p; null mutant sensitive to hydroxyurea (HU); green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; computational analysis suggests a role as a transcription factor
0.123 1.01E-12 YOL061W PRS5 hom 5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase; synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; forms cytoplasmic foci upon DNA replication stress