YMR100W / MUB1

MYND domain-containing protein required for ubiquitination and turnover of Rpn4p; interacts with Ubr2p (E3) and indirectly with Rad6p (E2); short-lived protein degraded in a Ubr2p/Rad6p dependent manner; similar to the A. nidulans samB gene

Zygosity: Homozygous strain
fixedexpanded
Profile for YMR100W / MUB1

Click on Significant Values for Screen Details ID:SGTC_36|Compound:0335-0881|FD-Score:-4.38|P-value:1.86E-5 ID:SGTC_41|Compound:0478-0632|FD-Score:3.87|P-value:1.48E-4 ID:SGTC_91|Compound:0417-1751|FD-Score:3.49|P-value:5.48E-4 ID:SGTC_102|Compound:gallobenzophenone|FD-Score:3.31|P-value:9.82E-4 ID:SGTC_257|Compound:daunorubicin|FD-Score:3.55|P-value:4.59E-4 ID:SGTC_304|Compound:1310-0298|FD-Score:-3.54|P-value:4.24E-4 ID:SGTC_306|Compound:1672-3436|FD-Score:3.50|P-value:5.41E-4 ID:SGTC_387|Compound:norethindrone acetate|FD-Score:4.22|P-value:3.96E-5 ID:SGTC_418|Compound:lividomycin a|FD-Score:-3.62|P-value:3.27E-4 ID:SGTC_513|Compound:4245-1575|FD-Score:-3.54|P-value:4.31E-4 ID:SGTC_568|Compound:1193-0097|FD-Score:4.70|P-value:5.51E-6 ID:SGTC_579|Compound:4491-1009|FD-Score:-4.31|P-value:2.47E-5 ID:SGTC_779|Compound:1360-0071|FD-Score:3.54|P-value:4.63E-4 ID:SGTC_785|Compound:1270-0111|FD-Score:3.69|P-value:2.85E-4 ID:SGTC_878|Compound:0828-0289|FD-Score:3.92|P-value:1.22E-4 ID:SGTC_950|Compound:1165-0508|FD-Score:3.77|P-value:2.14E-4 ID:SGTC_1149|Compound:3909-7961|FD-Score:4.38|P-value:2.06E-5 ID:SGTC_1255|Compound:0566-0045|FD-Score:-3.45|P-value:5.72E-4 ID:SGTC_1268|Compound:0828-0275|FD-Score:3.66|P-value:3.08E-4 ID:SGTC_1341|Compound:1486-1360|FD-Score:-3.37|P-value:7.64E-4 ID:SGTC_1403|Compound:3909-7815|FD-Score:-5.40|P-value:1.91E-7 ID:SGTC_1560|Compound:lithocholic acid|FD-Score:3.81|P-value:1.87E-4 ID:SGTC_1633|Compound:st005664|FD-Score:3.88|P-value:1.44E-4 ID:SGTC_1773|Compound:st048542|FD-Score:-3.52|P-value:4.65E-4 ID:SGTC_1779|Compound:st039427|FD-Score:3.52|P-value:5.05E-4 ID:SGTC_1789|Compound:tunicamycin|FD-Score:-4.13|P-value:5.06E-5 ID:SGTC_1932|Compound:st074806|FD-Score:3.44|P-value:6.57E-4 ID:SGTC_1941|Compound:st076221|FD-Score:-5.59|P-value:7.36E-8 ID:SGTC_1943|Compound:st076331|FD-Score:-6.28|P-value:1.75E-9 ID:SGTC_1947|Compound:st076332|FD-Score:-5.75|P-value:3.22E-8 ID:SGTC_2034|Compound:5197015|FD-Score:6.38|P-value:1.15E-9 ID:SGTC_2168|Compound:5727979|FD-Score:3.39|P-value:7.57E-4 ID:SGTC_2185|Compound:6113692|FD-Score:-3.91|P-value:1.16E-4 ID:SGTC_2273|Compound:7815444|FD-Score:4.93|P-value:1.95E-6 ID:SGTC_2311|Compound:7694451|FD-Score:-4.10|P-value:5.63E-5 ID:SGTC_2397|Compound:5161689|FD-Score:3.75|P-value:2.26E-4 ID:SGTC_2405|Compound:ticlatone|FD-Score:-4.11|P-value:5.41E-5 ID:SGTC_2543|Compound:pimpinellin|FD-Score:-3.77|P-value:1.92E-4 ID:SGTC_2564|Compound:betulinic acid|FD-Score:3.81|P-value:1.82E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:7.08|P-value:1.67E-11 ID:SGTC_2696|Compound:st077691|FD-Score:4.76|P-value:4.17E-6 ID:SGTC_2858|Compound:9025781|FD-Score:-3.83|P-value:1.57E-4 ID:SGTC_3059|Compound:1327-0034|FD-Score:-3.75|P-value:2.08E-4 ID:SGTC_36|Compound:0335-0881|FD-Score:-4.38|P-value:1.86E-5 ID:SGTC_41|Compound:0478-0632|FD-Score:3.87|P-value:1.48E-4 ID:SGTC_91|Compound:0417-1751|FD-Score:3.49|P-value:5.48E-4 ID:SGTC_102|Compound:gallobenzophenone|FD-Score:3.31|P-value:9.82E-4 ID:SGTC_257|Compound:daunorubicin|FD-Score:3.55|P-value:4.59E-4 ID:SGTC_304|Compound:1310-0298|FD-Score:-3.54|P-value:4.24E-4 ID:SGTC_306|Compound:1672-3436|FD-Score:3.50|P-value:5.41E-4 ID:SGTC_387|Compound:norethindrone acetate|FD-Score:4.22|P-value:3.96E-5 ID:SGTC_418|Compound:lividomycin a|FD-Score:-3.62|P-value:3.27E-4 ID:SGTC_513|Compound:4245-1575|FD-Score:-3.54|P-value:4.31E-4 ID:SGTC_568|Compound:1193-0097|FD-Score:4.70|P-value:5.51E-6 ID:SGTC_579|Compound:4491-1009|FD-Score:-4.31|P-value:2.47E-5 ID:SGTC_779|Compound:1360-0071|FD-Score:3.54|P-value:4.63E-4 ID:SGTC_785|Compound:1270-0111|FD-Score:3.69|P-value:2.85E-4 ID:SGTC_878|Compound:0828-0289|FD-Score:3.92|P-value:1.22E-4 ID:SGTC_950|Compound:1165-0508|FD-Score:3.77|P-value:2.14E-4 ID:SGTC_1149|Compound:3909-7961|FD-Score:4.38|P-value:2.06E-5 ID:SGTC_1255|Compound:0566-0045|FD-Score:-3.45|P-value:5.72E-4 ID:SGTC_1268|Compound:0828-0275|FD-Score:3.66|P-value:3.08E-4 ID:SGTC_1341|Compound:1486-1360|FD-Score:-3.37|P-value:7.64E-4 ID:SGTC_1403|Compound:3909-7815|FD-Score:-5.40|P-value:1.91E-7 ID:SGTC_1560|Compound:lithocholic acid|FD-Score:3.81|P-value:1.87E-4 ID:SGTC_1633|Compound:st005664|FD-Score:3.88|P-value:1.44E-4 ID:SGTC_1773|Compound:st048542|FD-Score:-3.52|P-value:4.65E-4 ID:SGTC_1779|Compound:st039427|FD-Score:3.52|P-value:5.05E-4 ID:SGTC_1789|Compound:tunicamycin|FD-Score:-4.13|P-value:5.06E-5 ID:SGTC_1932|Compound:st074806|FD-Score:3.44|P-value:6.57E-4 ID:SGTC_1941|Compound:st076221|FD-Score:-5.59|P-value:7.36E-8 ID:SGTC_1943|Compound:st076331|FD-Score:-6.28|P-value:1.75E-9 ID:SGTC_1947|Compound:st076332|FD-Score:-5.75|P-value:3.22E-8 ID:SGTC_2034|Compound:5197015|FD-Score:6.38|P-value:1.15E-9 ID:SGTC_2168|Compound:5727979|FD-Score:3.39|P-value:7.57E-4 ID:SGTC_2185|Compound:6113692|FD-Score:-3.91|P-value:1.16E-4 ID:SGTC_2273|Compound:7815444|FD-Score:4.93|P-value:1.95E-6 ID:SGTC_2311|Compound:7694451|FD-Score:-4.10|P-value:5.63E-5 ID:SGTC_2397|Compound:5161689|FD-Score:3.75|P-value:2.26E-4 ID:SGTC_2405|Compound:ticlatone|FD-Score:-4.11|P-value:5.41E-5 ID:SGTC_2543|Compound:pimpinellin|FD-Score:-3.77|P-value:1.92E-4 ID:SGTC_2564|Compound:betulinic acid|FD-Score:3.81|P-value:1.82E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:7.08|P-value:1.67E-11 ID:SGTC_2696|Compound:st077691|FD-Score:4.76|P-value:4.17E-6 ID:SGTC_2858|Compound:9025781|FD-Score:-3.83|P-value:1.57E-4 ID:SGTC_3059|Compound:1327-0034|FD-Score:-3.75|P-value:2.08E-4

Top fitness defect scores for YMR100W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2688 kanamycin b 7.08 96.7 µM 1.67E-11 calcium & mitochondrial duress 105 7.16E-13
2 SGTC_2034 5197015 6.38 53.1 µM 1.15E-9 SWF1 & branched chain AA biosynthesis 6 8.76E-11
3 SGTC_2273 7815444 4.93 169.1 µM 1.95E-6 9 4.04E-7
4 SGTC_2696 st077691 4.76 74.3 µM 4.17E-6 18 9.54E-7
5 SGTC_568 1193-0097 4.70 7.5 µM 5.51E-6 ERAD & cell cycle 103 1.31E-6
6 SGTC_1149 3909-7961 4.38 23.3 µM 2.06E-5 11 5.84E-6
7 SGTC_387 norethindrone acetate 4.22 66.7 µM 3.96E-5 9 1.22E-5
8 SGTC_878 0828-0289 3.92 13.7 µM 1.22E-4 49 4.36E-5
9 SGTC_1633 st005664 3.88 67.9 µM 1.44E-4 31 5.27E-5
10 SGTC_41 0478-0632 3.87 82.6 µM 1.48E-4 31 5.45E-5
11 SGTC_2564 betulinic acid 3.81 100.0 µM 1.82E-4 34 6.85E-5
12 SGTC_1560 lithocholic acid 3.81 53.1 µM 1.87E-4 6 7.07E-5
13 SGTC_950 1165-0508 3.77 94.1 µM 2.14E-4 34 8.25E-5
14 SGTC_2397 5161689 3.75 200.0 µM 2.26E-4 44 8.79E-5
15 SGTC_785 1270-0111 3.69 65.3 µM 2.85E-4 35 1.14E-4
16 SGTC_1268 0828-0275 3.66 2.5 µM 3.08E-4 30 1.25E-4
17 SGTC_257 daunorubicin 3.55 18.5 µM 4.59E-4 anthracycline transcription coupled DNA repair 31 1.96E-4
18 SGTC_779 1360-0071 3.54 233.0 µM 4.63E-4 fatty acid desaturase (OLE1) 49 1.98E-4
19 SGTC_1779 st039427 3.52 55.0 µM 5.05E-4 42 2.18E-4
20 SGTC_306 1672-3436 3.50 11.6 µM 5.41E-4 Golgi 23 2.35E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.133 9.95E-15 YBR287W_p YBR287W_p hom Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the ER; YBR287W is not an essential gene
0.123 7.80E-13 YCR075W-A_p YCR075W-A_p hom Putative protein of unknown function; identified by homology to Ashbya gossypii; YCR075W-A has a paralog, YNR034W-A, that arose from the whole genome duplication
0.122 1.46E-12 YDR101C ARX1 hom Shuttling pre-60S factor; involved in the biogenesis of ribosomal large subunit biogenesis; interacts directly with Alb1; responsible for Tif6 recycling defects in absence of Rei1; associated with the ribosomal export complex
0.121 1.99E-12 YLR024C UBR2 hom Cytoplasmic ubiquitin-protein ligase (E3); required for ubiquitylation of Rpn4p; mediates formation of a Mub1p-Ubr2p-Rad6p complex
0.120 2.57E-12 YDL052C SLC1 hom 1-acyl-sn-glycerol-3-phosphate acyltransferase, catalyzes the acylation of lysophosphatidic acid to form phosphatidic acid, a key intermediate in lipid metabolism; enzymatic activity detected in lipid particles and microsomes
0.104 1.60E-9 YGR135W PRE9 hom Alpha 3 subunit of the 20S proteasome, the only nonessential 20S subunit; may be replaced by the alpha 4 subunit (Pre6p) under stress conditions to create a more active proteasomal isoform
0.102 3.04E-9 YKL061W_p BLI1_p hom Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; green fluorescent protein (GFP)-fusion protein localizes to the endosome
0.099 8.37E-9 YER048C CAJ1 hom Nuclear type II J heat shock protein of the E. coli dnaJ family, contains a leucine zipper-like motif, binds to non-native substrates for presentation to Ssa3p, may function during protein translocation, assembly and disassembly
0.098 1.15E-8 YCR088W ABP1 hom Actin-binding protein of the cortical actin cytoskeleton, important for activation of the Arp2/3 complex that plays a key role actin in cytoskeleton organization; phosphorylation within its PRR (Proline-Rich Region), mediated by Cdc28p and Pho85p, protects Abp1p from proteolysis mediated by its own PEST sequences
0.098 1.37E-8 YMR099C YMR099C hom Glucose-6-phosphate 1-epimerase (hexose-6-phosphate mutarotase), likely involved in carbohydrate metabolism; GFP-fusion protein localizes to both the nucleus and cytoplasm and is induced in response to the DNA-damaging agent MMS
0.098 1.45E-8 YMR210W YMR210W hom Putative acyltransferase with similarity to Eeb1p and Eht1p, has a minor role in medium-chain fatty acid ethyl ester biosynthesis; may be involved in lipid metabolism and detoxification
0.098 1.54E-8 YLR443W ECM7 hom Non-essential putative integral membrane protein with a role in calcium uptake; mutant has cell wall defects and Ca+ uptake deficiencies; transcription is induced under conditions of zinc deficiency
0.094 4.80E-8 YIL135C VHS2 hom Cytoplasmic protein of unknown function; identified as a high-copy suppressor of the synthetic lethality of a sis2 sit4 double mutant, suggesting a role in G1/S phase progression; similar to Mlf3p
0.094 5.49E-8 YDR467C_d YDR467C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.092 9.87E-8 YBR048W RPS11B hom Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S11 and bacterial S17; RPS11B has a paralog, RPS11A, that arose from the whole genome duplication