YMR104C / YPK2

Protein kinase with similarity to serine/threonine protein kinase Ypk1p; functionally redundant with YPK1 at the genetic level; participates in a signaling pathway required for optimal cell wall integrity; homolog of mammalian kinase SGK; YPK2 has a paralog, YPK1, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YMR104C / YPK2

Click on Significant Values for Screen Details ID:SGTC_298|Compound:1391-0729|FD-Score:2.90|P-value:4.37E-4 ID:SGTC_299|Compound:1273-0059|FD-Score:2.76|P-value:7.51E-4 ID:SGTC_301|Compound:4119-0100|FD-Score:-2.92|P-value:5.84E-4 ID:SGTC_406|Compound:estrone|FD-Score:-3.00|P-value:4.24E-4 ID:SGTC_501|Compound:nsc-95397|FD-Score:-3.11|P-value:2.73E-4 ID:SGTC_504|Compound:lovastatin|FD-Score:-3.23|P-value:1.59E-4 ID:SGTC_510|Compound:ag-490|FD-Score:-3.07|P-value:3.13E-4 ID:SGTC_543|Compound:3844-1095|FD-Score:-3.36|P-value:9.24E-5 ID:SGTC_613|Compound:3914-0030|FD-Score:-4.07|P-value:2.73E-6 ID:SGTC_669|Compound:0108-0021|FD-Score:2.70|P-value:9.74E-4 ID:SGTC_716|Compound:1683-5115|FD-Score:2.91|P-value:4.13E-4 ID:SGTC_790|Compound:1575-0012|FD-Score:3.19|P-value:1.29E-4 ID:SGTC_798|Compound:1287-0182|FD-Score:4.93|P-value:9.66E-9 ID:SGTC_802|Compound:4112-3352|FD-Score:3.97|P-value:2.93E-6 ID:SGTC_808|Compound:0711-0032|FD-Score:2.77|P-value:7.25E-4 ID:SGTC_822|Compound:1487-0950|FD-Score:2.76|P-value:7.63E-4 ID:SGTC_996|Compound:3970-0799|FD-Score:4.77|P-value:2.66E-8 ID:SGTC_1107|Compound:amlodipine|FD-Score:-4.92|P-value:1.81E-8 ID:SGTC_1125|Compound:tnp00248|FD-Score:3.16|P-value:1.45E-4 ID:SGTC_1132|Compound:3456-3614|FD-Score:2.72|P-value:8.98E-4 ID:SGTC_1186|Compound:1319-0216|FD-Score:3.55|P-value:2.49E-5 ID:SGTC_1270|Compound:0831-1170|FD-Score:2.74|P-value:8.14E-4 ID:SGTC_1630|Compound:st005209|FD-Score:2.83|P-value:5.76E-4 ID:SGTC_1918|Compound:oxolan-2-ylmethyl 3-bromobenzoate|FD-Score:3.18|P-value:1.35E-4 ID:SGTC_1946|Compound:st076057|FD-Score:2.82|P-value:5.93E-4 ID:SGTC_2014|Compound:4013930|FD-Score:3.55|P-value:2.46E-5 ID:SGTC_2018|Compound:5133068|FD-Score:-3.32|P-value:1.08E-4 ID:SGTC_2330|Compound:7979373|FD-Score:-2.98|P-value:4.56E-4 ID:SGTC_2332|Compound:7982454|FD-Score:-3.89|P-value:7.23E-6 ID:SGTC_2582|Compound:agaric acid|FD-Score:-2.78|P-value:9.96E-4 ID:SGTC_2693|Compound:st077581|FD-Score:2.99|P-value:3.05E-4 ID:SGTC_2839|Compound:9003951|FD-Score:3.23|P-value:1.07E-4 ID:SGTC_2956|Compound:9076196|FD-Score:-3.20|P-value:1.82E-4 ID:SGTC_2961|Compound:9083290|FD-Score:-3.32|P-value:1.09E-4 ID:SGTC_3005|Compound:9076496|FD-Score:-3.24|P-value:1.52E-4 ID:SGTC_3018|Compound:9082602|FD-Score:3.49|P-value:3.24E-5 ID:SGTC_298|Compound:1391-0729|FD-Score:2.90|P-value:4.37E-4 ID:SGTC_299|Compound:1273-0059|FD-Score:2.76|P-value:7.51E-4 ID:SGTC_301|Compound:4119-0100|FD-Score:-2.92|P-value:5.84E-4 ID:SGTC_406|Compound:estrone|FD-Score:-3.00|P-value:4.24E-4 ID:SGTC_501|Compound:nsc-95397|FD-Score:-3.11|P-value:2.73E-4 ID:SGTC_504|Compound:lovastatin|FD-Score:-3.23|P-value:1.59E-4 ID:SGTC_510|Compound:ag-490|FD-Score:-3.07|P-value:3.13E-4 ID:SGTC_543|Compound:3844-1095|FD-Score:-3.36|P-value:9.24E-5 ID:SGTC_613|Compound:3914-0030|FD-Score:-4.07|P-value:2.73E-6 ID:SGTC_669|Compound:0108-0021|FD-Score:2.70|P-value:9.74E-4 ID:SGTC_716|Compound:1683-5115|FD-Score:2.91|P-value:4.13E-4 ID:SGTC_790|Compound:1575-0012|FD-Score:3.19|P-value:1.29E-4 ID:SGTC_798|Compound:1287-0182|FD-Score:4.93|P-value:9.66E-9 ID:SGTC_802|Compound:4112-3352|FD-Score:3.97|P-value:2.93E-6 ID:SGTC_808|Compound:0711-0032|FD-Score:2.77|P-value:7.25E-4 ID:SGTC_822|Compound:1487-0950|FD-Score:2.76|P-value:7.63E-4 ID:SGTC_996|Compound:3970-0799|FD-Score:4.77|P-value:2.66E-8 ID:SGTC_1107|Compound:amlodipine|FD-Score:-4.92|P-value:1.81E-8 ID:SGTC_1125|Compound:tnp00248|FD-Score:3.16|P-value:1.45E-4 ID:SGTC_1132|Compound:3456-3614|FD-Score:2.72|P-value:8.98E-4 ID:SGTC_1186|Compound:1319-0216|FD-Score:3.55|P-value:2.49E-5 ID:SGTC_1270|Compound:0831-1170|FD-Score:2.74|P-value:8.14E-4 ID:SGTC_1630|Compound:st005209|FD-Score:2.83|P-value:5.76E-4 ID:SGTC_1918|Compound:oxolan-2-ylmethyl 3-bromobenzoate|FD-Score:3.18|P-value:1.35E-4 ID:SGTC_1946|Compound:st076057|FD-Score:2.82|P-value:5.93E-4 ID:SGTC_2014|Compound:4013930|FD-Score:3.55|P-value:2.46E-5 ID:SGTC_2018|Compound:5133068|FD-Score:-3.32|P-value:1.08E-4 ID:SGTC_2330|Compound:7979373|FD-Score:-2.98|P-value:4.56E-4 ID:SGTC_2332|Compound:7982454|FD-Score:-3.89|P-value:7.23E-6 ID:SGTC_2582|Compound:agaric acid|FD-Score:-2.78|P-value:9.96E-4 ID:SGTC_2693|Compound:st077581|FD-Score:2.99|P-value:3.05E-4 ID:SGTC_2839|Compound:9003951|FD-Score:3.23|P-value:1.07E-4 ID:SGTC_2956|Compound:9076196|FD-Score:-3.20|P-value:1.82E-4 ID:SGTC_2961|Compound:9083290|FD-Score:-3.32|P-value:1.09E-4 ID:SGTC_3005|Compound:9076496|FD-Score:-3.24|P-value:1.52E-4 ID:SGTC_3018|Compound:9082602|FD-Score:3.49|P-value:3.24E-5

Top fitness defect scores for YMR104C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_798 1287-0182 4.93 476.0 µM 9.66E-9 9 4.15E-7
2 SGTC_996 3970-0799 4.77 1.8 µM 2.66E-8 60 9.18E-7
3 SGTC_802 4112-3352 3.97 742.0 µM 2.93E-6 25 3.66E-5
4 SGTC_2014 4013930 3.55 200.0 µM 2.46E-5 59 1.94E-4
5 SGTC_1186 1319-0216 3.55 26.0 µM 2.49E-5 31 1.96E-4
6 SGTC_3018 9082602 3.49 49.5 µM 3.24E-5 60 2.41E-4
7 SGTC_2839 9003951 3.23 26.0 µM 1.07E-4 35 6.17E-4
8 SGTC_790 1575-0012 3.19 79.1 µM 1.29E-4 TSC3-RPN4 61 7.12E-4
9 SGTC_1918 oxolan-2-ylmethyl 3-bromobenzoate 3.18 70.1 µM 1.35E-4 69 7.39E-4
10 SGTC_1125 tnp00248 3.16 1.3 µM 1.45E-4 83 7.81E-4
11 SGTC_2693 st077581 2.99 40.7 µM 3.05E-4 93 0.00140
12 SGTC_716 1683-5115 2.91 658.0 µM 4.13E-4 73 0.00178
13 SGTC_298 1391-0729 2.90 10.0 µM 4.37E-4 calcium & mitochondrial duress 47 0.00186
14 SGTC_1630 st005209 2.83 31.3 µM 5.76E-4 97 0.00231
15 SGTC_1946 st076057 2.82 65.1 µM 5.93E-4 34 0.00237
16 SGTC_808 0711-0032 2.77 135.0 µM 7.25E-4 69 0.00277
17 SGTC_299 1273-0059 2.76 1.4 µM 7.51E-4 77 0.00285
18 SGTC_822 1487-0950 2.76 154.0 µM 7.63E-4 63 0.00289
19 SGTC_1270 0831-1170 2.74 17.3 µM 8.14E-4 73 0.00304
20 SGTC_1132 3456-3614 2.72 2.5 µM 8.98E-4 tubulin folding & SWR complex 154 0.00328

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.274 1.69E-58 YML118W NGL3 hom 3'-5' exonuclease specific for poly-A RNAs; has a domain similar to a magnesium-dependent endonuclease motif in mRNA deadenylase Ccr4p; similar to Ngl1p; NGL3 has a paralog, NGL2, that arose from the whole genome duplication
0.272 4.73E-58 YOR037W CYC2 hom Mitochondrial peripheral inner membrane protein, contains a FAD cofactor in a domain exposed in the intermembrane space; exhibits redox activity in vitro; likely participates in ligation of heme to acytochromes c and c1 (Cyc1p and Cyt1p)
0.268 2.71E-56 YDL171C GLT1 hom NAD(+)-dependent glutamate synthase (GOGAT), synthesizes glutamate from glutamine and alpha-ketoglutarate; with Gln1p, forms the secondary pathway for glutamate biosynthesis from ammonia; expression regulated by nitrogen source
0.249 1.33E-48 YNL042W-B_p YNL042W-B_p hom Putative protein of unknown function
0.242 9.74E-46 YDR505C PSP1 hom Asn and gln rich protein of unknown function; high-copy suppressor of POL1 (DNA polymerase alpha) and partial suppressor of CDC2 (polymerase delta) and CDC6 (pre-RC loading factor) mutations; overexpression results in growth inhibition; PSP1 has a paralog, YLR177W, that arose from the whole genome duplication
0.238 2.25E-44 YJR127C RSF2 hom Zinc-finger protein; involved in transcriptional control of both nuclear and mitochondrial genes, many of which specify products required for glycerol-based growth, respiration, and other functions; RSF2 has a paralog, TDA9, that arose from the whole genome duplication; relocalizes from nucleus to cytoplasm upon DNA replication stress
0.205 4.18E-33 YDL099W BUG1 hom Cis-golgi localized protein involved in ER to Golgi transport; forms a complex with the mammalian GRASP65 homolog, Grh1p; mutants are compromised for the fusion of ER-derived vesicles with Golgi membranes
0.199 2.81E-31 YGL096W TOS8 hom Homeodomain-containing protein and putative transcription factor; found associated with chromatin; target of SBF transcription factor; induced during meiosis and under cell-damaging conditions; TOS8 has a paralog, CUP9, that arose from the whole genome duplication
0.199 3.30E-31 YKR023W_p YKR023W_p hom Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.193 1.78E-29 YGR149W_p YGR149W_p hom Putative protein of unknown function; predicted to be an integal membrane protein
0.191 9.28E-29 YBL036C YBL036C hom Putative non-specific single-domain racemase based on structural similarity; binds pyridoxal 5'-phosphate; expression of GFP-fusion protein induced in response to the DNA-damaging agent MMS
0.189 2.60E-28 YML084W_d YML084W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.186 1.34E-27 YBR207W FTH1 hom Putative high affinity iron transporter; involved in transport of intravacuolar stores of iron; forms complex with Fet5p; expression is regulated by iron; proposed to play indirect role in endocytosis; protein abundance increases in response to DNA replication stress
0.186 1.83E-27 YGL081W_p YGL081W_p hom Putative protein of unknown function; non-essential gene; interacts genetically with CHS5, a gene involved in chitin biosynthesis
0.183 9.38E-27 YLR016C PML1 hom Subunit of the RES complex, which is required for nuclear retention of unspliced pre-mRNAs; acts in the same pathway as Pml39p and Mlp1p