HIPHOP chemogenomics database

YMR145C / NDE1

Mitochondrial external NADH dehydrogenase, a type II NAD(P)H:quinone oxidoreductase that catalyzes the oxidation of cytosolic NADH; Nde1p and Nde2p provide cytosolic NADH to the mitochondrial respiratory chain

Zygosity: Homozygous strain

fixedexpanded
Profile for YMR145C / NDE1

Click on Significant Values for Screen Details

Top fitness defect scores for YMR145C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_2494	18-aminoabieta-8,11,13-triene sulfate	5.22	3.7 µM	4.24E-8		25	8.74E-8
2	SGTC_6	0844-0013	4.79	19.8 µM	4.56E-7		47	8.35E-7
3	SGTC_1630	st005209	4.78	31.3 µM	4.82E-7		11	8.80E-7
4	SGTC_2684	capsaicin	4.45	100.0 µM	2.53E-6		14	4.25E-6
5	SGTC_2723	salbutamol	4.32	83.6 µM	4.69E-6		6	7.65E-6
6	SGTC_2113	5491891	4.22	107.0 µM	7.74E-6	TSC3-RPN4	14	1.23E-5
7	SGTC_1918	oxolan-2-ylmethyl 3-bromobenzoate	4.02	70.1 µM	1.90E-5		23	2.89E-5
8	SGTC_857	0438-0306	3.78	1.4 µM	5.40E-5	copper-dependent oxidative stress	15	7.82E-5
9	SGTC_1546	5-methoxyflavanone	3.69	78.7 µM	7.81E-5		14	1.11E-4
10	SGTC_3108	9121785	3.66	49.5 µM	8.77E-5		21	1.24E-4
11	SGTC_2419	paclitaxel	3.56	166.5 µM	1.32E-4		61	1.83E-4
12	SGTC_2813	7971799	3.54	71.4 µM	1.43E-4		29	1.97E-4
13	SGTC_3298	9116858	3.48	71.4 µM	1.86E-4		23	2.54E-4
14	SGTC_1352	1496-0991	3.47	99.0 µM	1.87E-4	tubulin folding & SWR complex	42	2.56E-4
15	SGTC_2598	perillyl alcohol	3.43	100.0 µM	2.24E-4		56	3.03E-4
16	SGTC_3286	9117135	3.40	37.2 µM	2.53E-4		36	3.40E-4
17	SGTC_3109	9122853	3.39	49.5 µM	2.63E-4		21	3.54E-4
18	SGTC_160	3453-0671	3.36	289.7 µM	2.97E-4		60	3.97E-4
19	SGTC_3158	9100007	3.35	49.5 µM	3.02E-4		37	4.03E-4
20	SGTC_1449	4112-4002	3.30	201.0 µM	3.60E-4	RPP1 & pyrimidine depletion	29	4.76E-4

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.207	9.53E-34	YKL202W_d	YKL202W_d	hom	Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.176	9.78E-25	YKR069W	MET1	hom	S-adenosyl-L-methionine uroporphyrinogen III transmethylase, involved in the biosynthesis of siroheme, a prosthetic group used by sulfite reductase; required for sulfate assimilation and methionine biosynthesis
0.165	5.53E-22	YOR300W_d	YOR300W_d	hom	Dubious open reading frame, unlikely to encode a protein; overlaps with verified gene BUD7/YOR299W; mutation affects bipolar budding and bud site selection, though phenotype could be due to the mutation's effects on BUD7
0.144	5.26E-17	YLR288C	MEC3	hom	DNA damage and meiotic pachytene checkpoint protein; subunit of a heterotrimeric complex (Rad17p-Mec3p-Ddc1p) that forms a sliding clamp, loaded onto partial duplex DNA by a clamp loader complex; homolog of human and S. pombe Hus1
0.143	1.03E-16	YER067C-A_d	YER067C-A_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YER067W
0.139	5.92E-16	YDL049C	KNH1	hom	Protein with similarity to Kre9p, which is involved in cell wall beta 1,6-glucan synthesis; overproduction suppresses growth defects of a kre9 null mutant; required for propionic acid resistance
0.134	7.17E-15	YLR329W	REC102	hom	Protein involved in early stages of meiotic recombination; required for chromosome synapsis; forms a complex with Rec104p and Spo11p necessary during the initiation of recombination
0.132	1.63E-14	YLR398C	SKI2	hom	Ski complex component and putative RNA helicase, mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay
0.128	9.25E-14	YDL018C	ERP3	hom	Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport
0.122	1.52E-12	YGL037C	PNC1	hom	Nicotinamidase that converts nicotinamide to nicotinic acid; part of the NAD(+) salvage pathway; required for life span extension by calorie restriction; PNC1 expression responds to all known stimuli that extend replicative life span; protein increases in abundance and relative distribution to cytoplasmic foci decreases upon DNA replication stress
0.121	1.83E-12	YHR021W-A_p	ECM12_p	hom	Putative protein of unknown function; may contribute to cell wall biosynthesis, mutants display zymolyase hypersensitivity
0.121	2.19E-12	YOR155C	ISN1	hom	Inosine 5'-monophosphate (IMP)-specific 5'-nucleotidase, catalyzes the breakdown of IMP to inosine, does not show similarity to known 5'-nucleotidases from other organisms
0.120	2.83E-12	YBL086C_p	YBL086C_p	hom	Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery
0.120	2.88E-12	YKR009C	FOX2	hom	Multifunctional enzyme of the peroxisomal fatty acid beta-oxidation pathway; has 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities
0.119	5.02E-12	YKL122C	SRP21	het	Subunit of the signal recognition particle (SRP), which functions in protein targeting to the endoplasmic reticulum membrane; not found in mammalian SRP; forms a pre-SRP structure in the nucleolus that is translocated to the cytoplasm