YMR161W / HLJ1

Co-chaperone for Hsp40p, anchored in the ER membrane; with its homolog Ydj1p promotes ER-associated protein degradation (ERAD) of integral membrane substrates; similar to E. coli DnaJ

Zygosity: Homozygous strain
fixedexpanded
Profile for YMR161W / HLJ1

Click on Significant Values for Screen Details ID:SGTC_16|Compound:erodoxin|FD-Score:16.80|P-value:6.75E-59 ID:SGTC_28|Compound:pentachloropyridine N-oxide|FD-Score:16.40|P-value:7.14E-56 ID:SGTC_103|Compound:0844-0104|FD-Score:6.04|P-value:3.53E-9 ID:SGTC_133|Compound:0724-0033|FD-Score:-4.99|P-value:8.19E-7 ID:SGTC_238|Compound:5hr heat shock (37°C) + nsc-638432|FD-Score:3.89|P-value:9.63E-5 ID:SGTC_315|Compound:k915-0449|FD-Score:4.15|P-value:3.56E-5 ID:SGTC_405|Compound:ebelactone b|FD-Score:4.33|P-value:1.70E-5 ID:SGTC_470|Compound:aa-861|FD-Score:3.37|P-value:6.14E-4 ID:SGTC_513|Compound:4245-1575|FD-Score:6.84|P-value:2.71E-11 ID:SGTC_580|Compound:1123-0028|FD-Score:4.52|P-value:7.50E-6 ID:SGTC_585|Compound:0kpi-0023|FD-Score:3.62|P-value:2.62E-4 ID:SGTC_1090|Compound:efavirenz|FD-Score:4.22|P-value:2.59E-5 ID:SGTC_1137|Compound:ixabepilone|FD-Score:3.67|P-value:2.18E-4 ID:SGTC_1239|Compound:0337-0584|FD-Score:6.86|P-value:2.43E-11 ID:SGTC_1264|Compound:0783-0040|FD-Score:3.59|P-value:2.92E-4 ID:SGTC_1536|Compound:cytidine-2',3'-monophosphoric acid;  3'-cytidylic acid|FD-Score:3.50|P-value:4.03E-4 ID:SGTC_1640|Compound:st009078|FD-Score:3.68|P-value:2.12E-4 ID:SGTC_2015|Compound:5119600|FD-Score:-4.02|P-value:5.55E-5 ID:SGTC_2022|Compound:5136654|FD-Score:-5.28|P-value:1.95E-7 ID:SGTC_2050|Compound:5263056|FD-Score:3.55|P-value:3.39E-4 ID:SGTC_2078|Compound:5212016|FD-Score:3.78|P-value:1.45E-4 ID:SGTC_2245|Compound:6637752|FD-Score:3.91|P-value:9.13E-5 ID:SGTC_2402|Compound:5553605|FD-Score:-4.09|P-value:4.23E-5 ID:SGTC_2599|Compound:2',3-dihydroxy-4,4',6'-trimethoxychalcone|FD-Score:-3.65|P-value:2.28E-4 ID:SGTC_2607|Compound:solasodine|FD-Score:6.45|P-value:3.13E-10 ID:SGTC_2791|Compound:5663188|FD-Score:3.37|P-value:6.14E-4 ID:SGTC_2795|Compound:7780010|FD-Score:4.91|P-value:1.28E-6 ID:SGTC_2803|Compound:7707263|FD-Score:-5.02|P-value:7.22E-7 ID:SGTC_2997|Compound:9067075|FD-Score:6.44|P-value:3.30E-10 ID:SGTC_3044|Compound:9093193|FD-Score:-3.70|P-value:1.90E-4 ID:SGTC_3047|Compound:9092122|FD-Score:3.84|P-value:1.17E-4 ID:SGTC_3077|Compound:9117319|FD-Score:4.42|P-value:1.14E-5 ID:SGTC_3106|Compound:9121286|FD-Score:-3.48|P-value:4.19E-4 ID:SGTC_3199|Compound:9111318|FD-Score:4.29|P-value:1.96E-5 ID:SGTC_3208|Compound:9114115|FD-Score:4.10|P-value:4.27E-5 ID:SGTC_3218|Compound:9131285|FD-Score:-4.72|P-value:2.87E-6 ID:SGTC_3306|Compound:9127676|FD-Score:4.28|P-value:2.06E-5 ID:SGTC_3309|Compound:9129340|FD-Score:5.01|P-value:7.97E-7 ID:SGTC_3332|Compound:9141069|FD-Score:-4.65|P-value:4.06E-6 ID:SGTC_3335|Compound:9143315|FD-Score:3.73|P-value:1.79E-4 ID:SGTC_16|Compound:erodoxin|FD-Score:16.80|P-value:6.75E-59 ID:SGTC_28|Compound:pentachloropyridine N-oxide|FD-Score:16.40|P-value:7.14E-56 ID:SGTC_103|Compound:0844-0104|FD-Score:6.04|P-value:3.53E-9 ID:SGTC_133|Compound:0724-0033|FD-Score:-4.99|P-value:8.19E-7 ID:SGTC_238|Compound:5hr heat shock (37°C) + nsc-638432|FD-Score:3.89|P-value:9.63E-5 ID:SGTC_315|Compound:k915-0449|FD-Score:4.15|P-value:3.56E-5 ID:SGTC_405|Compound:ebelactone b|FD-Score:4.33|P-value:1.70E-5 ID:SGTC_470|Compound:aa-861|FD-Score:3.37|P-value:6.14E-4 ID:SGTC_513|Compound:4245-1575|FD-Score:6.84|P-value:2.71E-11 ID:SGTC_580|Compound:1123-0028|FD-Score:4.52|P-value:7.50E-6 ID:SGTC_585|Compound:0kpi-0023|FD-Score:3.62|P-value:2.62E-4 ID:SGTC_1090|Compound:efavirenz|FD-Score:4.22|P-value:2.59E-5 ID:SGTC_1137|Compound:ixabepilone|FD-Score:3.67|P-value:2.18E-4 ID:SGTC_1239|Compound:0337-0584|FD-Score:6.86|P-value:2.43E-11 ID:SGTC_1264|Compound:0783-0040|FD-Score:3.59|P-value:2.92E-4 ID:SGTC_1536|Compound:cytidine-2',3'-monophosphoric acid;  3'-cytidylic acid|FD-Score:3.50|P-value:4.03E-4 ID:SGTC_1640|Compound:st009078|FD-Score:3.68|P-value:2.12E-4 ID:SGTC_2015|Compound:5119600|FD-Score:-4.02|P-value:5.55E-5 ID:SGTC_2022|Compound:5136654|FD-Score:-5.28|P-value:1.95E-7 ID:SGTC_2050|Compound:5263056|FD-Score:3.55|P-value:3.39E-4 ID:SGTC_2078|Compound:5212016|FD-Score:3.78|P-value:1.45E-4 ID:SGTC_2245|Compound:6637752|FD-Score:3.91|P-value:9.13E-5 ID:SGTC_2402|Compound:5553605|FD-Score:-4.09|P-value:4.23E-5 ID:SGTC_2599|Compound:2',3-dihydroxy-4,4',6'-trimethoxychalcone|FD-Score:-3.65|P-value:2.28E-4 ID:SGTC_2607|Compound:solasodine|FD-Score:6.45|P-value:3.13E-10 ID:SGTC_2791|Compound:5663188|FD-Score:3.37|P-value:6.14E-4 ID:SGTC_2795|Compound:7780010|FD-Score:4.91|P-value:1.28E-6 ID:SGTC_2803|Compound:7707263|FD-Score:-5.02|P-value:7.22E-7 ID:SGTC_2997|Compound:9067075|FD-Score:6.44|P-value:3.30E-10 ID:SGTC_3044|Compound:9093193|FD-Score:-3.70|P-value:1.90E-4 ID:SGTC_3047|Compound:9092122|FD-Score:3.84|P-value:1.17E-4 ID:SGTC_3077|Compound:9117319|FD-Score:4.42|P-value:1.14E-5 ID:SGTC_3106|Compound:9121286|FD-Score:-3.48|P-value:4.19E-4 ID:SGTC_3199|Compound:9111318|FD-Score:4.29|P-value:1.96E-5 ID:SGTC_3208|Compound:9114115|FD-Score:4.10|P-value:4.27E-5 ID:SGTC_3218|Compound:9131285|FD-Score:-4.72|P-value:2.87E-6 ID:SGTC_3306|Compound:9127676|FD-Score:4.28|P-value:2.06E-5 ID:SGTC_3309|Compound:9129340|FD-Score:5.01|P-value:7.97E-7 ID:SGTC_3332|Compound:9141069|FD-Score:-4.65|P-value:4.06E-6 ID:SGTC_3335|Compound:9143315|FD-Score:3.73|P-value:1.79E-4

Top fitness defect scores for YMR161W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_16 erodoxin 16.80 22.8 µM 6.75E-59 unfolded protein response 7 7.38E-64
2 SGTC_28 pentachloropyridine N-oxide 16.40 1.6 µM 7.14E-56 TSC3-RPN4 2 1.43E-60
3 SGTC_1239 0337-0584 6.86 35.4 µM 2.43E-11 unfolded protein response 12 3.45E-12
4 SGTC_513 4245-1575 6.84 41.7 µM 2.71E-11 unfolded protein response 35 3.88E-12
5 SGTC_2607 solasodine 6.45 47.4 µM 3.13E-10 24 5.53E-11
6 SGTC_2997 9067075 6.44 71.4 µM 3.30E-10 unfolded protein response 14 5.87E-11
7 SGTC_103 0844-0104 6.04 51.8 µM 3.53E-9 ERAD & cell cycle 77 7.68E-10
8 SGTC_3309 9129340 5.01 25.8 µM 7.97E-7 5 2.75E-7
9 SGTC_2795 7780010 4.91 54.5 µM 1.28E-6 10 4.59E-7
10 SGTC_580 1123-0028 4.52 41.0 µM 7.50E-6 DNA damage response 20 3.13E-6
11 SGTC_3077 9117319 4.42 49.5 µM 1.14E-5 10 4.91E-6
12 SGTC_405 ebelactone b 4.33 50.0 µM 1.70E-5 10 7.57E-6
13 SGTC_3199 9111318 4.29 49.5 µM 1.96E-5 14 8.86E-6
14 SGTC_3306 9127676 4.28 59.8 µM 2.06E-5 14 9.36E-6
15 SGTC_1090 efavirenz 4.22 20.7 µM 2.59E-5 15 1.20E-5
16 SGTC_315 k915-0449 4.15 187.0 µM 3.56E-5 52 1.69E-5
17 SGTC_3208 9114115 4.10 49.5 µM 4.27E-5 29 2.06E-5
18 SGTC_2245 6637752 3.91 200.0 µM 9.13E-5 18 4.69E-5
19 SGTC_238 5hr heat shock (37°C) + nsc-638432 3.89 8.9 µM 9.63E-5 heat shock/prefoldin 45 4.97E-5
20 SGTC_3047 9092122 3.84 49.5 µM 1.17E-4 23 6.13E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.417 4.00E-141 YML130C ERO1 het Thiol oxidase required for oxidative protein folding in the ER; essential for maintaining proper redox balance in ER; feedback regulation of Ero1p occurs via reduction and oxidation of Ero1p regulatory bonds; reduced Pdi1p activates Ero1p by direct reduction of Ero1p regulatory bonds; depletion of thiol substrates and accumulation of oxidized Pdi1p results in inactivation of Ero1p by both Pdi1p-mediated oxidation and autonomous oxidation of Ero1p regulatory bonds
0.321 4.59E-81 YDL045C FAD1 het Flavin adenine dinucleotide (FAD) synthetase, performs the second step in synthesis of FAD from riboflavin
0.313 2.54E-77 YPL244C HUT1 hom Protein with a role in UDP-galactose transport to the Golgi lumen, has similarity to human UDP-galactose transporter UGTrel1, exhibits a genetic interaction with S. cerevisiae ERO1
0.283 1.04E-62 YFL031W HAC1 hom Basic leucine zipper (bZIP) transcription factor (ATF/CREB1 homolog); regulates the unfolded protein response, via UPRE binding, and membrane biogenesis; ER stress-induced splicing pathway facilitates efficient Hac1p synthesis; protein abundance increases in response to DNA replication stress
0.276 8.14E-60 YFL032W_d YFL032W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene HAC1/YFL031W; YFL032W is not an essential gene
0.266 2.40E-55 YHR079C IRE1 hom Serine-threonine kinase and endoribonuclease; transmembrane protein that mediates the unfolded protein response (UPR) by regulating Hac1p synthesis through HAC1 mRNA splicing; Kar2p binds inactive Ire1p and releases from it upon ER stress
0.213 9.19E-36 YJR117W STE24 hom Highly conserved zinc metalloprotease that functions in two steps of a-factor maturation, C-terminal CAAX proteolysis and the first step of N-terminal proteolytic processing; contains multiple transmembrane spans
0.213 9.99E-36 YGR089W NNF2 hom Protein that exhibits physical and genetic interactions with Rpb8p, which is a subunit of RNA polymerases I, II, and III; computational analysis of large-scale protein-protein interaction data suggests a role in chromosome segregation
0.200 1.12E-31 YLR292C SEC72 hom Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER
0.196 2.11E-30 YPL243W SRP68 het Core component of the signal recognition particle (SRP) complex; SRP complex functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress
0.189 2.26E-28 YEL043W YEL043W hom Predicted cytoskeleton protein involved in intracellular signalling based on quantitative analysis of protein-protein interaction maps; may interact with ribosomes, based on co-purification studies; contains fibronectin type III domain fold
0.186 2.03E-27 YML105C SEC65 het Subunit of the signal recognition particle (SRP), involved in protein targeting to the ER; interacts with Srp54p; homolog of mammalian SRP19
0.182 1.59E-26 YMR022W UBC7 hom Ubiquitin conjugating enzyme, involved in the ER-associated protein degradation pathway; requires Cue1p for recruitment to the ER membrane; proposed to be involved in chromatin assembly
0.161 7.95E-21 YPR074C TKL1 hom Transketolase; catalyzes conversion of xylulose-5-phosphate and ribose-5-phosphate to sedoheptulose-7-phosphate and glyceraldehyde-3-phosphate in the pentose phosphate pathway; needed for synthesis of aromatic amino acids; TKL1 has a paralog, TKL2, that arose from the whole genome duplication
0.160 1.42E-20 YCL052C PBN1 het Essential component of glycosylphosphatidylinositol-mannosyltransferase I, required for the autocatalytic post-translational processing of the protease B precursor Prb1p, localizes to ER in lumenal orientation; homolog of mammalian PIG-X