YMR185W / RTP1

Protein required for the nuclear import and biogenesis of RNA pol II; conflicting evidence on whether null mutant is viable with elongated buds, or inviable; interacts with Rpb2, Rpb3, Nup116p, Nup100p and components of the R2TP complex (Rvb1p, Rvb2p, Pih1p); similar to human TMCO7 gene

Zygosity: Heterozygous strain
fixedexpanded
Profile for YMR185W / RTP1

Click on Significant Values for Screen Details

Top fitness defect scores for YMR185W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature		Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2573 2',4'-dihydroxychalcone 10.40 15.0 µM 3.20E-28 3 1.21E-25 3.44
2 SGTC_2526 patulin 10.40 37.6 µM 4.01E-28 1 1.49E-25 2.13
3 SGTC_1069 diphenylcyclopropenone 9.85 8.5 µM 1.68E-25 2 3.46E-23 0.80
4 SGTC_611 3453-1652 8.75 23.7 µM 1.63E-20 4 1.10E-18 1.78
5 SGTC_879 0831-1030 8.06 162.0 µM 1.11E-17 3 3.97E-16 1.72
6 SGTC_210 3551-0445 7.88 56.8 µM 5.53E-17 4 1.69E-15 0.67
7 SGTC_3282 9108498 7.56 15.5 µM 8.72E-16 3 2.04E-14 0.62
8 SGTC_533 1222-0222 7.44 6.7 µM 2.32E-15 PDR1 1 4.94E-14 1.96
9 SGTC_2534 helenine 7.33 20.1 µM 5.88E-15 RSC complex & mRNA processing 8 1.15E-13 0.18
10 SGTC_2408 sulbentine 7.21 18.4 µM 1.54E-14 13 2.73E-13 0.32
11 SGTC_820 1582-0060 7.04 59.9 µM 6.10E-14 5 9.47E-13 0.62
12 SGTC_639 0833-0042 6.60 114.0 µM 1.78E-12 3 2.00E-11 2.35
13 SGTC_897 1000-0139 6.31 31.4 µM 1.54E-11 4 1.40E-10 0.11
14 SGTC_3290 9121031 6.28 71.4 µM 1.95E-11 1 1.74E-10 1.76
15 SGTC_2004 4023728 6.01 41.9 µM 1.23E-10 15 9.16E-10 0.23
16 SGTC_845 0330-0138 5.94 4.5 µM 2.00E-10 1 1.42E-9 0.75
17 SGTC_975 1335-0046 5.88 28.5 µM 3.06E-10 RPP1 & pyrimidine depletion 3 2.09E-9 1.42
18 SGTC_1978 st071761 5.55 42.0 µM 2.59E-9 TSC3-RPN4 3 1.45E-8 1.07
19 SGTC_589 1227-0070 5.52 34.1 µM 3.17E-9 4 1.73E-8 0.86
20 SGTC_2151 5563267 5.37 85.4 µM 7.90E-9 10 3.96E-8 0.46

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.452 2.25E-168 YLR243W GPN3 het Putative GTPase with a role in biogenesis of RNA pol II and polIII; may be involved in assembly of RNA polymerases II and III and in their transport into the nucleus; contains a Gly-Pro-Asn motif in the G domain; similar to Npa3p and Gpn2p
0.427 7.55E-149 YDR527W RBA50 het Protein involved in transcription; interacts with RNA polymerase II subunits Rpb2p, Rpb3, and Rpb11p; has similarity to human RPAP1
0.413 2.67E-138 YPR085C ASA1 het Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1
0.339 3.42E-91 YOR262W GPN2 het Putative GTPase with a role in biogenesis of RNA pol II and polIII; may be involved in assembly of RNA polymerases II and III and in their transport into the nucleus; contains a Gly-Pro-Asn motif in the G domain; similar to Npa3p and Gpn3p; highly conserved across species and homologous to human gene GPN2/ATPBD1B; required for establishment of sister chromatid cohesion
0.330 1.22E-85 YAL024C LTE1 hom Protein similar to GDP/GTP exchange factors but without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures
0.306 1.77E-73 YOR207C RET1 het Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs
0.236 7.96E-44 YML124C TUB3 hom Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication
0.220 6.89E-38 YOR349W CIN1 hom Tubulin folding factor D involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl
0.215 2.41E-36 YBR058C-A TSC3 hom Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
0.193 1.59E-29 YNL148C ALF1 hom Alpha-tubulin folding protein, similar to mammalian cofactor B; Alf1p-GFP localizes to cytoplasmic microtubules; required for the folding of alpha-tubulin and may play an additional role in microtubule maintenance
0.192 3.37E-29 YPL241C CIN2 hom GTPase-activating protein (GAP) for Cin4p; tubulin folding factor C involved in beta-tubulin (Tub2p) folding; mutants display increased chromosome loss and benomyl sensitivity; deletion complemented by human GAP, retinitis pigmentosa 2
0.192 4.59E-29 YNL298W CLA4 hom Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p
0.176 8.46E-25 YER107C GLE2 hom RNA export factor associated with the nuclear pore complex (NPC); associates with NUP116p; required for polyadenylated RNA export but not for protein import; homologous to S. pombe Rae1p and human RAE1
0.158 4.31E-20 YER007W PAC2 hom Microtubule effector required for tubulin heterodimer formation, binds alpha-tubulin, required for normal microtubule function, null mutant exhibits cold-sensitive microtubules and sensitivity to benomyl
0.134 6.67E-15 YFL023W BUD27 hom Unconventional prefoldin protein involved in translation initiation; mutants have inappropriate expression of nutrient sensitive genes due to translational derepression of Gcn4p transcription factor; diploid mutants show random budding; ortholog of human URI/RMP