YMR271C / URA10

Minor orotate phosphoribosyltransferase (OPRTase) isozyme; catalyzes the fifth enzymatic step in the de novo biosynthesis of pyrimidines, converting orotate into orotidine-5'-phosphate; URA10 has a paralog, URA5, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YMR271C / URA10

Click on Significant Values for Screen Details ID:SGTC_205|Compound:1486-1330|FD-Score:3.33|P-value:1.82E-4 ID:SGTC_247|Compound:mechlorethamine|FD-Score:2.93|P-value:8.45E-4 ID:SGTC_354|Compound:2783-3500|FD-Score:2.97|P-value:7.45E-4 ID:SGTC_430|Compound:0958-0115|FD-Score:3.01|P-value:6.36E-4 ID:SGTC_567|Compound:1080-0542|FD-Score:2.90|P-value:9.51E-4 ID:SGTC_579|Compound:4491-1009|FD-Score:5.36|P-value:5.56E-9 ID:SGTC_581|Compound:0958-0271|FD-Score:-3.89|P-value:2.08E-5 ID:SGTC_872|Compound:0588-0139|FD-Score:3.47|P-value:1.04E-4 ID:SGTC_918|Compound:glabranin|FD-Score:3.80|P-value:2.42E-5 ID:SGTC_922|Compound:1866-0035|FD-Score:-3.15|P-value:4.63E-4 ID:SGTC_1294|Compound:1000-1494|FD-Score:-3.25|P-value:3.22E-4 ID:SGTC_1314|Compound:1222-0010|FD-Score:3.23|P-value:2.75E-4 ID:SGTC_1596|Compound:piperlongumine|FD-Score:3.51|P-value:8.63E-5 ID:SGTC_1696|Compound:st025591|FD-Score:-3.12|P-value:5.18E-4 ID:SGTC_1715|Compound:st032160|FD-Score:3.62|P-value:5.50E-5 ID:SGTC_1716|Compound:st033144|FD-Score:3.42|P-value:1.29E-4 ID:SGTC_1742|Compound:st038235|FD-Score:3.10|P-value:4.59E-4 ID:SGTC_1847|Compound:st056231|FD-Score:-3.32|P-value:2.41E-4 ID:SGTC_1849|Compound:st056239|FD-Score:3.38|P-value:1.50E-4 ID:SGTC_1860|Compound:st056228|FD-Score:5.27|P-value:9.63E-9 ID:SGTC_1941|Compound:st076221|FD-Score:3.63|P-value:5.28E-5 ID:SGTC_1958|Compound:st076513|FD-Score:3.48|P-value:9.93E-5 ID:SGTC_2010|Compound:4012422|FD-Score:3.51|P-value:8.69E-5 ID:SGTC_2071|Compound:5230951|FD-Score:2.98|P-value:7.26E-4 ID:SGTC_2074|Compound:5212007|FD-Score:5.62|P-value:1.05E-9 ID:SGTC_2075|Compound:5212009|FD-Score:3.99|P-value:1.02E-5 ID:SGTC_2084|Compound:5306078|FD-Score:3.14|P-value:3.86E-4 ID:SGTC_2219|Compound:9098279|FD-Score:3.02|P-value:6.27E-4 ID:SGTC_2230|Compound:bromochlorosalicylanilide|FD-Score:3.07|P-value:5.11E-4 ID:SGTC_2238|Compound:6671620|FD-Score:3.54|P-value:7.65E-5 ID:SGTC_2240|Compound:6690706|FD-Score:-3.71|P-value:4.76E-5 ID:SGTC_2294|Compound:7971431|FD-Score:-3.31|P-value:2.45E-4 ID:SGTC_2452|Compound:5863136|FD-Score:3.25|P-value:2.57E-4 ID:SGTC_2580|Compound:diallyl disulphide|FD-Score:3.29|P-value:2.21E-4 ID:SGTC_2703|Compound:st077852|FD-Score:8.94|P-value:9.92E-22 ID:SGTC_2736|Compound:butenafine|FD-Score:-3.28|P-value:2.86E-4 ID:SGTC_3204|Compound:9114183|FD-Score:3.25|P-value:2.56E-4 ID:SGTC_3225|Compound:9130819|FD-Score:-3.01|P-value:7.70E-4 ID:SGTC_3310|Compound:9130229|FD-Score:-2.96|P-value:9.51E-4 ID:SGTC_205|Compound:1486-1330|FD-Score:3.33|P-value:1.82E-4 ID:SGTC_247|Compound:mechlorethamine|FD-Score:2.93|P-value:8.45E-4 ID:SGTC_354|Compound:2783-3500|FD-Score:2.97|P-value:7.45E-4 ID:SGTC_430|Compound:0958-0115|FD-Score:3.01|P-value:6.36E-4 ID:SGTC_567|Compound:1080-0542|FD-Score:2.90|P-value:9.51E-4 ID:SGTC_579|Compound:4491-1009|FD-Score:5.36|P-value:5.56E-9 ID:SGTC_581|Compound:0958-0271|FD-Score:-3.89|P-value:2.08E-5 ID:SGTC_872|Compound:0588-0139|FD-Score:3.47|P-value:1.04E-4 ID:SGTC_918|Compound:glabranin|FD-Score:3.80|P-value:2.42E-5 ID:SGTC_922|Compound:1866-0035|FD-Score:-3.15|P-value:4.63E-4 ID:SGTC_1294|Compound:1000-1494|FD-Score:-3.25|P-value:3.22E-4 ID:SGTC_1314|Compound:1222-0010|FD-Score:3.23|P-value:2.75E-4 ID:SGTC_1596|Compound:piperlongumine|FD-Score:3.51|P-value:8.63E-5 ID:SGTC_1696|Compound:st025591|FD-Score:-3.12|P-value:5.18E-4 ID:SGTC_1715|Compound:st032160|FD-Score:3.62|P-value:5.50E-5 ID:SGTC_1716|Compound:st033144|FD-Score:3.42|P-value:1.29E-4 ID:SGTC_1742|Compound:st038235|FD-Score:3.10|P-value:4.59E-4 ID:SGTC_1847|Compound:st056231|FD-Score:-3.32|P-value:2.41E-4 ID:SGTC_1849|Compound:st056239|FD-Score:3.38|P-value:1.50E-4 ID:SGTC_1860|Compound:st056228|FD-Score:5.27|P-value:9.63E-9 ID:SGTC_1941|Compound:st076221|FD-Score:3.63|P-value:5.28E-5 ID:SGTC_1958|Compound:st076513|FD-Score:3.48|P-value:9.93E-5 ID:SGTC_2010|Compound:4012422|FD-Score:3.51|P-value:8.69E-5 ID:SGTC_2071|Compound:5230951|FD-Score:2.98|P-value:7.26E-4 ID:SGTC_2074|Compound:5212007|FD-Score:5.62|P-value:1.05E-9 ID:SGTC_2075|Compound:5212009|FD-Score:3.99|P-value:1.02E-5 ID:SGTC_2084|Compound:5306078|FD-Score:3.14|P-value:3.86E-4 ID:SGTC_2219|Compound:9098279|FD-Score:3.02|P-value:6.27E-4 ID:SGTC_2230|Compound:bromochlorosalicylanilide|FD-Score:3.07|P-value:5.11E-4 ID:SGTC_2238|Compound:6671620|FD-Score:3.54|P-value:7.65E-5 ID:SGTC_2240|Compound:6690706|FD-Score:-3.71|P-value:4.76E-5 ID:SGTC_2294|Compound:7971431|FD-Score:-3.31|P-value:2.45E-4 ID:SGTC_2452|Compound:5863136|FD-Score:3.25|P-value:2.57E-4 ID:SGTC_2580|Compound:diallyl disulphide|FD-Score:3.29|P-value:2.21E-4 ID:SGTC_2703|Compound:st077852|FD-Score:8.94|P-value:9.92E-22 ID:SGTC_2736|Compound:butenafine|FD-Score:-3.28|P-value:2.86E-4 ID:SGTC_3204|Compound:9114183|FD-Score:3.25|P-value:2.56E-4 ID:SGTC_3225|Compound:9130819|FD-Score:-3.01|P-value:7.70E-4 ID:SGTC_3310|Compound:9130229|FD-Score:-2.96|P-value:9.51E-4

Top fitness defect scores for YMR271C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2703 st077852 8.94 53.4 µM 9.92E-22 1 2.01E-19
2 SGTC_2074 5212007 5.62 75.4 µM 1.05E-9 4 9.46E-9
3 SGTC_579 4491-1009 5.36 110.0 µM 5.56E-9 60S ribosome export 4 4.17E-8
4 SGTC_1860 st056228 5.27 74.6 µM 9.63E-9 2 6.79E-8
5 SGTC_2075 5212009 3.99 200.0 µM 1.02E-5 8 3.30E-5
6 SGTC_918 glabranin 3.80 770.7 nM 2.42E-5 37 7.11E-5
7 SGTC_1941 st076221 3.63 61.8 µM 5.28E-5 sphingolipid biosynthesis & PDR1 93 1.42E-4
8 SGTC_1715 st032160 3.62 10.2 µM 5.50E-5 54 1.48E-4
9 SGTC_2238 6671620 3.54 73.2 µM 7.65E-5 42 1.98E-4
10 SGTC_1596 piperlongumine 3.51 63.0 µM 8.63E-5 27 2.20E-4
11 SGTC_2010 4012422 3.51 159.0 µM 8.69E-5 35 2.22E-4
12 SGTC_1958 st076513 3.48 41.9 µM 9.93E-5 60S ribosome export 47 2.49E-4
13 SGTC_872 0588-0139 3.47 234.0 µM 1.04E-4 47 2.60E-4
14 SGTC_1716 st033144 3.42 30.6 µM 1.29E-4 37 3.14E-4
15 SGTC_1849 st056239 3.38 19.2 µM 1.50E-4 RPP1 & pyrimidine depletion 51 3.59E-4
16 SGTC_205 1486-1330 3.33 71.9 µM 1.82E-4 31 4.28E-4
17 SGTC_2580 diallyl disulphide 3.29 81.4 µM 2.21E-4 42 5.09E-4
18 SGTC_3204 9114183 3.25 49.5 µM 2.56E-4 62 5.80E-4
19 SGTC_2452 5863136 3.25 48.4 µM 2.57E-4 49 5.82E-4
20 SGTC_1314 1222-0010 3.23 41.1 µM 2.75E-4 34 6.16E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.113 4.89E-11 YLR423C ATG17 hom Scaffold protein responsible for phagophore assembly site organization; regulatory subunit of an autophagy-specific complex that includes Atg1p and Atg13p; stimulates Atg1p kinase activity; human ortholog RB1CC1/FIP200 interacts with p53, which inhibits autophagy in human cells
0.111 1.18E-10 YBR054W YRO2 hom Protein of unknown function with similarity to archaeal rhodopsins; the authentic, non-tagged protein is detected in a phosphorylated state in highly purified mitochondria in high-throughput studies; transcriptionally regulated by Haa1p; YRO2 has a paralog, MRH1, that arose from the whole genome duplication
0.104 1.66E-9 YMR285C NGL2 hom Protein involved in 5.8S rRNA processing; Ccr4p-like RNase required for correct 3'-end formation of 5.8S rRNA at site E; similar to Ngl1p; NGL2 has a paralog, NGL3, that arose from the whole genome duplication
0.098 1.29E-8 YDL012C YDL012C hom Tail-anchored plasma membrane protein with a conserved CYSTM module; possibly involved in response to stress; may contribute to non-homologous end-joining (NHEJ) based on ydl012c htz1 double null phenotype; YDL012C has a paralog, YBR016W, that arose from the whole genome duplication
0.095 3.76E-8 YMR306C-A_d YMR306C-A_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.088 3.77E-7 YLR281C_p YLR281C_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YLR281C is not an essential gene
0.087 4.16E-7 YPR145W ASN1 hom Asparagine synthetase; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway; ASN1 has a paralog, ASN2, that arose from the whole genome duplication
0.086 6.23E-7 YPL026C SKS1 hom Putative serine/threonine protein kinase; involved in the adaptation to low concentrations of glucose independent of the SNF3 regulated pathway
0.086 6.30E-7 YHR088W RPF1 het Nucleolar protein involved in the assembly and export of the large ribosomal subunit; constituent of 66S pre-ribosomal particles; contains a sigma(70)-like motif, which is thought to bind RNA
0.085 7.21E-7 YMR129W POM152 hom Glycoprotein subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication; homologous to human NUP210
0.085 8.26E-7 YJL016W_p YJL016W_p hom Putative protein of unknown function; GFP-fusion protein localizes to the cytoplasm; conserved in closely related Saccharomyces species
0.082 1.97E-6 YLR013W GAT3 hom Protein containing GATA family zinc finger motifs
0.081 2.41E-6 YGL099W LSG1 het Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm
0.081 2.59E-6 YBR150C TBS1 hom Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; TBS1 has a paralog, HAL9, that arose from the whole genome duplication
0.080 3.31E-6 YLR040C_p YLR040C_p hom Protein of unknown function; localizes to the cell wall; predicted to be a GPI-attached protein; upregulated by Mcm1p-Alpha1p transcription factor; partially overlaps the dubious ORF YLR041W; mRNA identified as translated by ribosome profiling data; YLR040C is not essential