HIPHOP chemogenomics database

YMR286W / MRPL33

Mitochondrial ribosomal protein of the large subunit

Zygosity: Homozygous strain

fixedexpanded
Profile for YMR286W / MRPL33

Click on Significant Values for Screen Details

Top fitness defect scores for YMR286W deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_236	1509-0011	31.50	5.3 µM	7.87E-202		11	2.17E-218
2	SGTC_3211	9128781	11.60	49.5 µM	2.69E-29		4	1.91E-31
3	SGTC_386	1509-0011	10.10	3.1 µM	9.44E-23		44	2.28E-24
4	SGTC_2460	5552655	7.82	174.2 µM	2.39E-14	amide catabolism	5	2.72E-15
5	SGTC_2250	alverine citrate	7.79	130.0 µM	2.93E-14		22	3.39E-15
6	SGTC_310	1273-0058	6.82	1.1 µM	2.28E-11	mitochondrial stress	122	4.45E-12
7	SGTC_908	3448-7491	6.71	47.7 µM	4.65E-11	mitochondrial stress	33	9.60E-12
8	SGTC_1188	1403-0014	5.86	4.6 µM	7.41E-9	mitochondrial stress	17	2.27E-9
9	SGTC_59	0330-0160	5.84	1.3 µM	8.45E-9		40	2.61E-9
10	SGTC_145	3737-0113	5.70	38.4 µM	1.81E-8	DNA intercalators	30	5.92E-9
11	SGTC_1926	methyl fluorone black	5.59	53.6 µM	3.38E-8	DNA intercalators	24	1.16E-8
12	SGTC_852	0418-0143	5.51	439.0 µM	4.99E-8	calcium & mitochondrial duress	33	1.76E-8
13	SGTC_275	trichlorophene	5.48	5.7 µM	6.05E-8	mitochondrial stress	139	2.17E-8
14	SGTC_144	4161-2749	5.48	112.4 µM	6.06E-8	DNA intercalators	7	2.17E-8
15	SGTC_1371	2421-0008	5.02	32.7 µM	5.98E-7	mitochondrial processes	14	2.55E-7
16	SGTC_1635	st007035	5.00	42.4 µM	6.79E-7		169	2.92E-7
17	SGTC_143	4032-1194	4.98	15.7 µM	7.37E-7		31	3.19E-7
18	SGTC_63	0099-0242	4.81	26.7 µM	1.62E-6	mitochondrial stress	63	7.44E-7
19	SGTC_134	0269-0018	4.36	6.9 µM	1.24E-5		116	6.60E-6
20	SGTC_184	1494-0393	4.19	24.7 µM	2.50E-5		51	1.40E-5

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.675	0	YOR330C	MIP1	hom	Mitochondrial DNA polymerase; conserved C-terminal segment is required for the maintenance of mitochondrial genome; mutations in the human ortholog POLG are associated with Alpers-Huttenlocher syndrome (AHS) and other mitochondrial diseases; Mip1p is the single subunit of mitochondrial DNA polymerase in yeast, in contrast to metazoans in which there is a complex of a catalytic subunit and an accessory subunit
0.618	0	YDR518W	EUG1	hom	Protein disulfide isomerase of the endoplasmic reticulum lumen; EUG1 has a paralog, PDI1, that arose from the whole genome duplication; function overlaps with that of Pdi1p; may interact with nascent polypeptides in the ER
0.618	0	YJL023C	PET130	hom	Protein required for respiratory growth; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.603	0	YDR230W_d	YDR230W_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene COX20
0.595	2.55E-320	YER141W	COX15	hom	Protein required for the hydroxylation of heme O to form heme A, which is an essential prosthetic group for cytochrome c oxidase
0.593	6.23E-318	YJL166W	QCR8	hom	Subunit 8 of ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the intermembrane space; expression is regulated by Abf1p and Cpf1p
0.591	5.61E-315	YMR035W	IMP2	hom	Catalytic subunit of the mitochondrial inner membrane peptidase complex, required for maturation of mitochondrial proteins of the intermembrane space; complex contains Imp1p and Imp2p (both catalytic subunits), and Som1p
0.588	3.36E-310	YPL215W	CBP3	hom	Mitochondrial protein required for assembly of cytochrome bc1 complex; forms a complex with Cbp6p that binds to mt ribosomes near the polypeptide tunnel exit and promotes efficient translation of the COB mRNA; Cbp3p-Cbp6p complex also interacts with newly synthesized cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp into the cytochrome bc1 complex
0.578	2.26E-298	YGR220C	MRPL9	hom	Mitochondrial ribosomal protein of the large subunit
0.573	1.09E-291	YPL013C	MRPS16	hom	Mitochondrial ribosomal protein of the small subunit
0.569	2.63E-286	YNL252C	MRPL17	hom	Mitochondrial ribosomal protein of the large subunit
0.564	1.00E-279	YJR120W	YJR120W	hom	Protein of unknown function; essential for growth under anaerobic conditions; mutation causes decreased expression of ATP2, impaired respiration, defective sterol uptake, and altered levels/localization of ABC transporters Aus1p and Pdr11p
0.561	3.81E-277	YML129C	COX14	hom	Mitochondrial membrane protein, involved in translational regulation of Cox1p and assembly of cytochrome c oxidase (complex IV); associates with complex IV assembly intermediates and complex III/complex IV supercomplexes
0.557	7.18E-273	YJL022W_d	YJL022W_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene PET130
0.550	2.47E-264	YDR079W	PET100	hom	Chaperone that specifically facilitates the assembly of cytochrome c oxidase, integral to the mitochondrial inner membrane; interacts with a subcomplex of subunits VII, VIIa, and VIII (Cox7p, Cox9p, and Cox8p) but not with the holoenzyme