YNL009W / IDP3

Peroxisomal NADP-dependent isocitrate dehydrogenase; catalyzes oxidation of isocitrate to alpha-ketoglutarate with the formation of NADP(H+), required for growth on unsaturated fatty acids; IDP3 has a paralog, IDP2, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YNL009W / IDP3

Click on Significant Values for Screen Details ID:SGTC_20|Compound:0481-0013|FD-Score:3.44|P-value:2.85E-4 ID:SGTC_95|Compound:0471-0001|FD-Score:3.54|P-value:1.96E-4 ID:SGTC_133|Compound:0724-0033|FD-Score:-3.40|P-value:4.07E-4 ID:SGTC_151|Compound:3013-0144|FD-Score:3.38|P-value:3.54E-4 ID:SGTC_263|Compound:alizarin|FD-Score:3.37|P-value:3.68E-4 ID:SGTC_341|Compound:1486-1430|FD-Score:3.24|P-value:5.80E-4 ID:SGTC_346|Compound:4513-0042|FD-Score:5.91|P-value:1.76E-9 ID:SGTC_350|Compound:k064-0020|FD-Score:3.88|P-value:5.21E-5 ID:SGTC_422|Compound:cantharidin|FD-Score:3.22|P-value:6.29E-4 ID:SGTC_473|Compound:2-arachidonoylglycerol|FD-Score:3.15|P-value:7.79E-4 ID:SGTC_660|Compound:4031-1742|FD-Score:3.36|P-value:3.78E-4 ID:SGTC_1038|Compound:k292-0204|FD-Score:3.74|P-value:8.91E-5 ID:SGTC_1129|Compound:3453-1384|FD-Score:4.13|P-value:1.80E-5 ID:SGTC_1290|Compound:0986-0249|FD-Score:3.70|P-value:1.03E-4 ID:SGTC_1512|Compound:0259-0544|FD-Score:-4.17|P-value:1.92E-5 ID:SGTC_1528|Compound:arabinosylhypoxanthine|FD-Score:3.91|P-value:4.53E-5 ID:SGTC_1565|Compound:sinapyl alcohol|FD-Score:3.43|P-value:2.98E-4 ID:SGTC_1770|Compound:st043884|FD-Score:3.14|P-value:8.31E-4 ID:SGTC_1910|Compound:st062352|FD-Score:3.79|P-value:7.26E-5 ID:SGTC_1936|Compound:st074407|FD-Score:3.32|P-value:4.30E-4 ID:SGTC_1947|Compound:st076332|FD-Score:-3.39|P-value:4.17E-4 ID:SGTC_1950|Compound:st077106|FD-Score:3.25|P-value:5.67E-4 ID:SGTC_1953|Compound:st076800|FD-Score:-4.30|P-value:1.08E-5 ID:SGTC_2006|Compound:4011487|FD-Score:-4.10|P-value:2.64E-5 ID:SGTC_2009|Compound:4012297|FD-Score:3.25|P-value:5.54E-4 ID:SGTC_2340|Compound:7987427|FD-Score:3.41|P-value:3.15E-4 ID:SGTC_2412|Compound:st077135|FD-Score:3.73|P-value:9.42E-5 ID:SGTC_2607|Compound:solasodine|FD-Score:-3.61|P-value:1.85E-4 ID:SGTC_2631|Compound:bromo-3-hydroxy-4-(succin-2-yl)-caryolane gamma-lactone|FD-Score:4.15|P-value:1.61E-5 ID:SGTC_2778|Compound:tioconazole|FD-Score:3.74|P-value:9.15E-5 ID:SGTC_2910|Compound:7948405|FD-Score:3.21|P-value:6.53E-4 ID:SGTC_2978|Compound:9011784|FD-Score:-3.47|P-value:3.09E-4 ID:SGTC_3153|Compound:9100258|FD-Score:3.37|P-value:3.70E-4 ID:SGTC_3211|Compound:9128781|FD-Score:-3.31|P-value:5.62E-4 ID:SGTC_3235|Compound:9132602|FD-Score:3.17|P-value:7.44E-4 ID:SGTC_3269|Compound:9137823|FD-Score:3.34|P-value:4.00E-4 ID:SGTC_3337|Compound:9144342|FD-Score:-3.48|P-value:3.06E-4 ID:SGTC_20|Compound:0481-0013|FD-Score:3.44|P-value:2.85E-4 ID:SGTC_95|Compound:0471-0001|FD-Score:3.54|P-value:1.96E-4 ID:SGTC_133|Compound:0724-0033|FD-Score:-3.40|P-value:4.07E-4 ID:SGTC_151|Compound:3013-0144|FD-Score:3.38|P-value:3.54E-4 ID:SGTC_263|Compound:alizarin|FD-Score:3.37|P-value:3.68E-4 ID:SGTC_341|Compound:1486-1430|FD-Score:3.24|P-value:5.80E-4 ID:SGTC_346|Compound:4513-0042|FD-Score:5.91|P-value:1.76E-9 ID:SGTC_350|Compound:k064-0020|FD-Score:3.88|P-value:5.21E-5 ID:SGTC_422|Compound:cantharidin|FD-Score:3.22|P-value:6.29E-4 ID:SGTC_473|Compound:2-arachidonoylglycerol|FD-Score:3.15|P-value:7.79E-4 ID:SGTC_660|Compound:4031-1742|FD-Score:3.36|P-value:3.78E-4 ID:SGTC_1038|Compound:k292-0204|FD-Score:3.74|P-value:8.91E-5 ID:SGTC_1129|Compound:3453-1384|FD-Score:4.13|P-value:1.80E-5 ID:SGTC_1290|Compound:0986-0249|FD-Score:3.70|P-value:1.03E-4 ID:SGTC_1512|Compound:0259-0544|FD-Score:-4.17|P-value:1.92E-5 ID:SGTC_1528|Compound:arabinosylhypoxanthine|FD-Score:3.91|P-value:4.53E-5 ID:SGTC_1565|Compound:sinapyl alcohol|FD-Score:3.43|P-value:2.98E-4 ID:SGTC_1770|Compound:st043884|FD-Score:3.14|P-value:8.31E-4 ID:SGTC_1910|Compound:st062352|FD-Score:3.79|P-value:7.26E-5 ID:SGTC_1936|Compound:st074407|FD-Score:3.32|P-value:4.30E-4 ID:SGTC_1947|Compound:st076332|FD-Score:-3.39|P-value:4.17E-4 ID:SGTC_1950|Compound:st077106|FD-Score:3.25|P-value:5.67E-4 ID:SGTC_1953|Compound:st076800|FD-Score:-4.30|P-value:1.08E-5 ID:SGTC_2006|Compound:4011487|FD-Score:-4.10|P-value:2.64E-5 ID:SGTC_2009|Compound:4012297|FD-Score:3.25|P-value:5.54E-4 ID:SGTC_2340|Compound:7987427|FD-Score:3.41|P-value:3.15E-4 ID:SGTC_2412|Compound:st077135|FD-Score:3.73|P-value:9.42E-5 ID:SGTC_2607|Compound:solasodine|FD-Score:-3.61|P-value:1.85E-4 ID:SGTC_2631|Compound:bromo-3-hydroxy-4-(succin-2-yl)-caryolane gamma-lactone|FD-Score:4.15|P-value:1.61E-5 ID:SGTC_2778|Compound:tioconazole|FD-Score:3.74|P-value:9.15E-5 ID:SGTC_2910|Compound:7948405|FD-Score:3.21|P-value:6.53E-4 ID:SGTC_2978|Compound:9011784|FD-Score:-3.47|P-value:3.09E-4 ID:SGTC_3153|Compound:9100258|FD-Score:3.37|P-value:3.70E-4 ID:SGTC_3211|Compound:9128781|FD-Score:-3.31|P-value:5.62E-4 ID:SGTC_3235|Compound:9132602|FD-Score:3.17|P-value:7.44E-4 ID:SGTC_3269|Compound:9137823|FD-Score:3.34|P-value:4.00E-4 ID:SGTC_3337|Compound:9144342|FD-Score:-3.48|P-value:3.06E-4

Top fitness defect scores for YNL009W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_346 4513-0042 5.91 7.2 µM 1.76E-9 azole & statin 11 1.68E-9
2 SGTC_2631 bromo-3-hydroxy-4-(succin-2-yl)-caryolane gamma-lactone 4.15 100.0 µM 1.61E-5 32 1.64E-5
3 SGTC_1129 3453-1384 4.13 223.0 µM 1.80E-5 34 1.83E-5
4 SGTC_1528 arabinosylhypoxanthine 3.91 74.6 µM 4.53E-5 21 4.62E-5
5 SGTC_350 k064-0020 3.88 19.8 µM 5.21E-5 heme requiring 30 5.32E-5
6 SGTC_1910 st062352 3.79 60.4 µM 7.26E-5 43 7.42E-5
7 SGTC_1038 k292-0204 3.74 154.0 µM 8.91E-5 23 9.12E-5
8 SGTC_2778 tioconazole 3.74 65.1 nM 9.15E-5 ergosterol depletion effects on membrane 72 9.37E-5
9 SGTC_2412 st077135 3.73 33.4 µM 9.42E-5 30 9.65E-5
10 SGTC_1290 0986-0249 3.70 10.2 µM 1.03E-4 DNA intercalators 29 1.06E-4
11 SGTC_95 0471-0001 3.54 561.1 µM 1.96E-4 39 2.01E-4
12 SGTC_20 0481-0013 3.44 4.9 µM 2.85E-4 45 2.94E-4
13 SGTC_1565 sinapyl alcohol 3.43 95.1 µM 2.98E-4 50 3.06E-4
14 SGTC_2340 7987427 3.41 183.4 µM 3.15E-4 59 3.24E-4
15 SGTC_151 3013-0144 3.38 2.3 µM 3.54E-4 iron homeostasis 50 3.65E-4
16 SGTC_263 alizarin 3.37 107.9 µM 3.68E-4 iron homeostasis 91 3.79E-4
17 SGTC_3153 9100258 3.37 49.5 µM 3.70E-4 48 3.81E-4
18 SGTC_660 4031-1742 3.36 102.0 µM 3.78E-4 redox potentiating 76 3.89E-4
19 SGTC_3269 9137823 3.34 49.5 µM 4.00E-4 60S ribosome export 34 4.12E-4
20 SGTC_1936 st074407 3.32 74.3 µM 4.30E-4 DNA damage response 63 4.43E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.154 3.22E-19 YNR003C RPC34 het RNA polymerase III subunit C34; interacts with TFIIIB70 and is a key determinant in pol III recruitment by the preinitiation complex
0.141 2.94E-16 YDR032C PST2 hom Protein with similarity to a family of flavodoxin-like proteins; induced by oxidative stress in a Yap1p dependent manner; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress; PST2 has a paralog, RFS1, that arose from the whole genome duplication
0.116 1.64E-11 YAL014C SYN8 hom Endosomal SNARE related to mammalian syntaxin 8
0.112 8.71E-11 YKL067W YNK1 hom Nucleoside diphosphate kinase; catalyzes the transfer of gamma phosphates from nucleoside triphosphates, usually ATP, to nucleoside diphosphates by a mechanism that involves formation of an autophosphorylated enzyme intermediate; protein abundance increases in response to DNA replication stress
0.106 8.27E-10 YGR184C UBR1 hom E3 ubiquitin ligase (N-recognin); forms heterodimer with Rad6p to ubiquitinate substrates in the N-end rule pathway; regulates peptide transport via Cup9p ubiquitination; plays a major role in targeting cytosolic misfolded proteins for degradation; mutation in human UBR1 causes Johansson-Blizzard Syndrome (JBS)
0.104 1.56E-9 YLR004C THI73 hom Putative plasma membrane permease proposed to be involved in carboxylic acid uptake and repressed by thiamine; substrate of Dbf2p/Mob1p kinase; transcription is altered if mitochondrial dysfunction occurs
0.102 4.05E-9 YBR060C ORC2 het Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; interacts with Spp1p and with trimethylated histone H3; phosphorylated by Cdc28p
0.101 4.38E-9 YKL116C PRR1 hom Serine/threonine protein kinase that inhibits pheromone induced signalling downstream of MAPK, possibly at the level of the Ste12p transcription factor
0.095 3.90E-8 YBR101C FES1 hom Hsp70 (Ssa1p) nucleotide exchange factor; cytosolic homolog of Sil1p, which is the nucleotide exchange factor for BiP (Kar2p) in the endoplasmic reticulum; protein abundance increases in response to DNA replication stress
0.081 2.52E-6 YMR173W-A_d YMR173W-A_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene DDR48/YML173W
0.081 2.85E-6 YBR281C DUG2 hom Component of glutamine amidotransferase (GATase II); forms a complex with Dug3p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p)
0.081 2.92E-6 YJL093C TOK1 hom Outward-rectifier potassium channel of the plasma membrane with two pore domains in tandem, each of which forms a functional channel permeable to potassium; carboxy tail functions to prevent inner gate closures; target of K1 toxin
0.079 5.15E-6 YAL018C_p YAL018C_p hom Putative protein of unknown function
0.078 6.82E-6 YNL256W FOL1 het Multifunctional enzyme of the folic acid biosynthesis pathway, has dihydropteroate synthetase, dihydro-6-hydroxymethylpterin pyrophosphokinase, and dihydroneopterin aldolase activities
0.077 8.12E-6 YER168C CCA1 het ATP (CTP):tRNA-specific tRNA nucleotidyltransferase; different forms targeted to the nucleus, cytosol, and mitochondrion are generated via the use of multiple transcriptional and translational start sites