YNL016W / PUB1

Poly (A)+ RNA-binding protein; abundant mRNP-component protein that binds mRNA and is required for stability of many mRNAs; component of glucose deprivation induced stress granules, involved in P-body-dependent granule assembly; protein abundance increases in response to DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YNL016W / PUB1

Click on Significant Values for Screen Details ID:SGTC_58|Compound:0957-0393|FD-Score:11.30|P-value:1.39E-28 ID:SGTC_64|Compound:000l-0018|FD-Score:4.38|P-value:8.22E-6 ID:SGTC_102|Compound:gallobenzophenone|FD-Score:4.57|P-value:3.61E-6 ID:SGTC_103|Compound:0844-0104|FD-Score:4.63|P-value:2.79E-6 ID:SGTC_121|Compound:0557-0495|FD-Score:-3.81|P-value:1.56E-4 ID:SGTC_138|Compound:0850-0259|FD-Score:6.33|P-value:2.99E-10 ID:SGTC_337|Compound:1315-0038|FD-Score:3.39|P-value:4.06E-4 ID:SGTC_359|Compound:0081-0058|FD-Score:4.91|P-value:7.17E-7 ID:SGTC_385|Compound:1494-0393|FD-Score:3.52|P-value:2.53E-4 ID:SGTC_414|Compound:piperonyl butoxide|FD-Score:4.77|P-value:1.45E-6 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:3.34|P-value:4.80E-4 ID:SGTC_652|Compound:0437-0092|FD-Score:3.23|P-value:6.95E-4 ID:SGTC_671|Compound:0560-0003|FD-Score:3.84|P-value:7.71E-5 ID:SGTC_673|Compound:1082-0474|FD-Score:4.45|P-value:6.18E-6 ID:SGTC_678|Compound:1218-2179|FD-Score:-3.43|P-value:6.00E-4 ID:SGTC_682|Compound:0kpi-0099|FD-Score:4.09|P-value:2.85E-5 ID:SGTC_733|Compound:4069-0007|FD-Score:5.94|P-value:3.10E-9 ID:SGTC_948|Compound:3448-0295|FD-Score:3.24|P-value:6.86E-4 ID:SGTC_1053|Compound:3366-3204|FD-Score:3.17|P-value:8.53E-4 ID:SGTC_1161|Compound:k007-0610|FD-Score:4.05|P-value:3.38E-5 ID:SGTC_1424|Compound:4048-1941|FD-Score:3.60|P-value:1.95E-4 ID:SGTC_1546|Compound:5-methoxyflavanone|FD-Score:-3.43|P-value:5.97E-4 ID:SGTC_1671|Compound:st016596|FD-Score:3.39|P-value:4.12E-4 ID:SGTC_1729|Compound:st035615|FD-Score:3.18|P-value:8.31E-4 ID:SGTC_1796|Compound:st049173|FD-Score:3.52|P-value:2.55E-4 ID:SGTC_2008|Compound:4012253|FD-Score:3.32|P-value:5.23E-4 ID:SGTC_2021|Compound:4019055|FD-Score:-3.29|P-value:9.53E-4 ID:SGTC_2023|Compound:5144124|FD-Score:3.28|P-value:5.99E-4 ID:SGTC_2030|Compound:5175110|FD-Score:-3.49|P-value:4.87E-4 ID:SGTC_2038|Compound:4100038|FD-Score:-5.97|P-value:6.20E-9 ID:SGTC_2227|Compound:7312221|FD-Score:-3.45|P-value:5.69E-4 ID:SGTC_2246|Compound:7293507|FD-Score:3.45|P-value:3.35E-4 ID:SGTC_2408|Compound:sulbentine|FD-Score:-4.25|P-value:2.73E-5 ID:SGTC_2420|Compound:aureobasidin a|FD-Score:4.50|P-value:4.88E-6 ID:SGTC_2707|Compound:st078556|FD-Score:3.20|P-value:7.65E-4 ID:SGTC_2819|Compound:7996655|FD-Score:3.60|P-value:1.92E-4 ID:SGTC_2834|Compound:9001755|FD-Score:-5.45|P-value:1.07E-7 ID:SGTC_2846|Compound:9008642|FD-Score:4.99|P-value:4.88E-7 ID:SGTC_2853|Compound:9015042|FD-Score:3.26|P-value:6.39E-4 ID:SGTC_2856|Compound:9022925|FD-Score:3.35|P-value:4.64E-4 ID:SGTC_2880|Compound:9042983|FD-Score:3.29|P-value:5.77E-4 ID:SGTC_2963|Compound:9082507|FD-Score:-3.28|P-value:9.74E-4 ID:SGTC_3045|Compound:9092950|FD-Score:3.44|P-value:3.40E-4 ID:SGTC_3054|Compound:9094397|FD-Score:-5.40|P-value:1.35E-7 ID:SGTC_3061|Compound:glucosamine|FD-Score:4.40|P-value:7.55E-6 ID:SGTC_3143|Compound:9086658|FD-Score:-3.67|P-value:2.58E-4 ID:SGTC_3218|Compound:9131285|FD-Score:5.15|P-value:2.21E-7 ID:SGTC_3275|Compound:9138069|FD-Score:-3.72|P-value:2.16E-4 ID:SGTC_3337|Compound:9144342|FD-Score:-3.60|P-value:3.36E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:11.30|P-value:1.39E-28 ID:SGTC_64|Compound:000l-0018|FD-Score:4.38|P-value:8.22E-6 ID:SGTC_102|Compound:gallobenzophenone|FD-Score:4.57|P-value:3.61E-6 ID:SGTC_103|Compound:0844-0104|FD-Score:4.63|P-value:2.79E-6 ID:SGTC_121|Compound:0557-0495|FD-Score:-3.81|P-value:1.56E-4 ID:SGTC_138|Compound:0850-0259|FD-Score:6.33|P-value:2.99E-10 ID:SGTC_337|Compound:1315-0038|FD-Score:3.39|P-value:4.06E-4 ID:SGTC_359|Compound:0081-0058|FD-Score:4.91|P-value:7.17E-7 ID:SGTC_385|Compound:1494-0393|FD-Score:3.52|P-value:2.53E-4 ID:SGTC_414|Compound:piperonyl butoxide|FD-Score:4.77|P-value:1.45E-6 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:3.34|P-value:4.80E-4 ID:SGTC_652|Compound:0437-0092|FD-Score:3.23|P-value:6.95E-4 ID:SGTC_671|Compound:0560-0003|FD-Score:3.84|P-value:7.71E-5 ID:SGTC_673|Compound:1082-0474|FD-Score:4.45|P-value:6.18E-6 ID:SGTC_678|Compound:1218-2179|FD-Score:-3.43|P-value:6.00E-4 ID:SGTC_682|Compound:0kpi-0099|FD-Score:4.09|P-value:2.85E-5 ID:SGTC_733|Compound:4069-0007|FD-Score:5.94|P-value:3.10E-9 ID:SGTC_948|Compound:3448-0295|FD-Score:3.24|P-value:6.86E-4 ID:SGTC_1053|Compound:3366-3204|FD-Score:3.17|P-value:8.53E-4 ID:SGTC_1161|Compound:k007-0610|FD-Score:4.05|P-value:3.38E-5 ID:SGTC_1424|Compound:4048-1941|FD-Score:3.60|P-value:1.95E-4 ID:SGTC_1546|Compound:5-methoxyflavanone|FD-Score:-3.43|P-value:5.97E-4 ID:SGTC_1671|Compound:st016596|FD-Score:3.39|P-value:4.12E-4 ID:SGTC_1729|Compound:st035615|FD-Score:3.18|P-value:8.31E-4 ID:SGTC_1796|Compound:st049173|FD-Score:3.52|P-value:2.55E-4 ID:SGTC_2008|Compound:4012253|FD-Score:3.32|P-value:5.23E-4 ID:SGTC_2021|Compound:4019055|FD-Score:-3.29|P-value:9.53E-4 ID:SGTC_2023|Compound:5144124|FD-Score:3.28|P-value:5.99E-4 ID:SGTC_2030|Compound:5175110|FD-Score:-3.49|P-value:4.87E-4 ID:SGTC_2038|Compound:4100038|FD-Score:-5.97|P-value:6.20E-9 ID:SGTC_2227|Compound:7312221|FD-Score:-3.45|P-value:5.69E-4 ID:SGTC_2246|Compound:7293507|FD-Score:3.45|P-value:3.35E-4 ID:SGTC_2408|Compound:sulbentine|FD-Score:-4.25|P-value:2.73E-5 ID:SGTC_2420|Compound:aureobasidin a|FD-Score:4.50|P-value:4.88E-6 ID:SGTC_2707|Compound:st078556|FD-Score:3.20|P-value:7.65E-4 ID:SGTC_2819|Compound:7996655|FD-Score:3.60|P-value:1.92E-4 ID:SGTC_2834|Compound:9001755|FD-Score:-5.45|P-value:1.07E-7 ID:SGTC_2846|Compound:9008642|FD-Score:4.99|P-value:4.88E-7 ID:SGTC_2853|Compound:9015042|FD-Score:3.26|P-value:6.39E-4 ID:SGTC_2856|Compound:9022925|FD-Score:3.35|P-value:4.64E-4 ID:SGTC_2880|Compound:9042983|FD-Score:3.29|P-value:5.77E-4 ID:SGTC_2963|Compound:9082507|FD-Score:-3.28|P-value:9.74E-4 ID:SGTC_3045|Compound:9092950|FD-Score:3.44|P-value:3.40E-4 ID:SGTC_3054|Compound:9094397|FD-Score:-5.40|P-value:1.35E-7 ID:SGTC_3061|Compound:glucosamine|FD-Score:4.40|P-value:7.55E-6 ID:SGTC_3143|Compound:9086658|FD-Score:-3.67|P-value:2.58E-4 ID:SGTC_3218|Compound:9131285|FD-Score:5.15|P-value:2.21E-7 ID:SGTC_3275|Compound:9138069|FD-Score:-3.72|P-value:2.16E-4 ID:SGTC_3337|Compound:9144342|FD-Score:-3.60|P-value:3.36E-4

Top fitness defect scores for YNL016W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_58 0957-0393 11.30 9.0 µM 1.39E-28 cell wall 59 4.06E-30
2 SGTC_138 0850-0259 6.33 40.9 µM 2.99E-10 cell wall 99 1.20E-10
3 SGTC_733 4069-0007 5.94 56.1 µM 3.10E-9 ERAD & cell cycle 40 1.42E-9
4 SGTC_3218 9131285 5.15 49.5 µM 2.21E-7 endomembrane recycling 13 1.29E-7
5 SGTC_2846 9008642 4.99 19.5 µM 4.88E-7 9 2.97E-7
6 SGTC_359 0081-0058 4.91 15.9 µM 7.17E-7 60S ribosome export 21 4.45E-7
7 SGTC_414 piperonyl butoxide 4.77 100.0 µM 1.45E-6 2 9.37E-7
8 SGTC_103 0844-0104 4.63 51.8 µM 2.79E-6 ERAD & cell cycle 114 1.87E-6
9 SGTC_102 gallobenzophenone 4.57 83.6 µM 3.61E-6 redox potentiating 33 2.45E-6
10 SGTC_2420 aureobasidin a 4.50 50.0 nM 4.88E-6 28 3.36E-6
11 SGTC_673 1082-0474 4.45 110.0 µM 6.18E-6 RSC complex & mRNA processing 43 4.31E-6
12 SGTC_3061 glucosamine 4.40 200.0 µM 7.55E-6 4 5.31E-6
13 SGTC_64 000l-0018 4.38 272.1 µM 8.22E-6 ubiquinone biosynthesis & proteasome 127 5.81E-6
14 SGTC_682 0kpi-0099 4.09 17.0 µM 2.85E-5 66 2.15E-5
15 SGTC_1161 k007-0610 4.05 110.0 µM 3.38E-5 ERAD & cell cycle 115 2.57E-5
16 SGTC_671 0560-0003 3.84 407.0 µM 7.71E-5 12 6.11E-5
17 SGTC_2819 7996655 3.60 46.4 µM 1.92E-4 29 1.59E-4
18 SGTC_1424 4048-1941 3.60 275.0 µM 1.95E-4 33 1.62E-4
19 SGTC_385 1494-0393 3.52 12.4 µM 2.53E-4 40 2.12E-4
20 SGTC_1796 st049173 3.52 110.4 µM 2.55E-4 24 2.14E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.279 1.12E-60 YDR205W MSC2 hom Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Zrg17p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; localizes to ER and nucleus; mutations affect the cellular distribution of zinc and also confer defects in meiotic recombination between homologous chromatids
0.263 3.48E-54 YOR231W MKK1 hom MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk2p; MKK1 has a paralog, MKK2, that arose from the whole genome duplication
0.256 4.10E-51 YLR150W STM1 hom Protein required for optimal translation under nutrient stress; perturbs association of Yef3p with ribosomes; involved in TOR signaling; binds G4 quadruplex and purine motif triplex nucleic acid; helps maintain telomere structure; protein abundance increases in response to DNA replication stress; serves as a ribosome preservation factor both during quiescence and recovery
0.256 4.56E-51 YGR058W PEF1 hom Penta-EF-hand protein required for polar bud growth and cell wall abscission; binds calcium and zinc with different affinity; localizes to bud site in G1, bud neck in G2; binds to Sec31p and modulates COPII coat assembly
0.252 1.79E-49 YKR042W UTH1 hom Protein involved in cell wall biogenesis; required for mitochondrial autophagy; mitochondrial outer membrane and cell wall localized SUN family member; involved in the oxidative stress response, life span during starvation, and cell death; UTH1 has a paralog, NCA3, that arose from the whole genome duplication
0.249 1.13E-48 YJR054W KCH1 hom Potassium transporter that mediates K+ influx; activates the high-affinity Ca2+ influx system (HACS) during the mating pheromone response; expression is up-regulated in response to alpha factor; localized to sites of polarized growth; similar to Prm6p; member of a fungal-specific gene family; potential Cdc28p substrate
0.248 3.22E-48 YAL015C NTG1 hom DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase; involved in base excision repair; acts in both nucleus and mitochondrion; creates a double-strand break at mtDNA origins that stimulates replication in response to oxidative stress; NTG1 has a paralog, NTG2, that arose from the whole genome duplication
0.247 1.08E-47 YDR537C_d YDR537C_d hom Dubious open reading frame unlikely to encode a protein, almost completely overlaps verified ORF PAD1/YDR538W
0.220 7.09E-38 YML057W CMP2 hom Calcineurin A; one isoform (the other is CNA1) of the catalytic subunit of calcineurin, a Ca++/calmodulin-regulated protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is CNB1
0.209 1.81E-34 YLR331C_d JIP3_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene MID2
0.208 3.40E-34 YCL037C SRO9 hom Cytoplasmic RNA-binding protein; associates with translating ribosomes; involved in heme regulation of Hap1p as a component of the HMC complex, also involved in the organization of actin filaments; contains a La motif; SRO9 has a paralog, SLF1, that arose from the whole genome duplication
0.208 6.33E-34 YJL193W_p YJL193W_p hom Putative protein of unknown function, predicted to encode a triose phosphate transporter subfamily member based on phylogenetic analysis; similar to YOR307C/SLY41; deletion mutant has a respiratory growth defect
0.206 1.46E-33 YNL179C_d YNL179C_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; deletion in cyr1 mutant results in loss of stress resistance
0.205 4.72E-33 YML057C-A_d YML057C-A_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene CMP2/YML057W
0.204 5.81E-33 YJR142W_p YJR142W_p hom Putative protein of unknown function; GST fusion protein is a Dbf2p-Mob1p phosphorylation target in a proteome chip analysis; synthetic lethal with PH085 deletion; plays a role in restricting Ty1 transposition; nudix hydrolase family member