YNL025C / SSN8

Cyclin-like component of the RNA polymerase II holoenzyme, involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression and telomere maintenance

Zygosity: Homozygous strain
fixedexpanded
Profile for YNL025C / SSN8

Click on Significant Values for Screen Details ID:SGTC_126|Compound:0417-1624|FD-Score:3.43|P-value:8.00E-4 ID:SGTC_176|Compound:1486-1328|FD-Score:4.59|P-value:1.18E-5 ID:SGTC_187|Compound:1488-2104|FD-Score:8.62|P-value:1.15E-15 ID:SGTC_241|Compound:5hr heat shock (37°C) + cantharidin|FD-Score:5.80|P-value:4.79E-8 ID:SGTC_262|Compound:fenpropimorph|FD-Score:4.15|P-value:6.70E-5 ID:SGTC_445|Compound:e6 berbamine|FD-Score:5.41|P-value:3.20E-7 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-7.90|P-value:2.08E-13 ID:SGTC_582|Compound:0987-0078|FD-Score:3.66|P-value:3.74E-4 ID:SGTC_649|Compound:1123-0311|FD-Score:3.91|P-value:1.60E-4 ID:SGTC_770|Compound:0389-1121|FD-Score:5.21|P-value:8.23E-7 ID:SGTC_831|Compound:k061-1017|FD-Score:3.81|P-value:2.25E-4 ID:SGTC_872|Compound:0588-0139|FD-Score:6.13|P-value:8.40E-9 ID:SGTC_1022|Compound:4477-0945|FD-Score:3.91|P-value:1.59E-4 ID:SGTC_1047|Compound:2043-3044|FD-Score:5.75|P-value:6.10E-8 ID:SGTC_1145|Compound:3546-0597|FD-Score:4.11|P-value:7.77E-5 ID:SGTC_1199|Compound:1638-0010|FD-Score:4.36|P-value:3.01E-5 ID:SGTC_1322|Compound:1348-1070|FD-Score:-3.54|P-value:5.94E-4 ID:SGTC_1330|Compound:1469-0054|FD-Score:7.51|P-value:2.41E-12 ID:SGTC_1335|Compound:1486-0900|FD-Score:5.80|P-value:4.72E-8 ID:SGTC_1402|Compound:3909-7673|FD-Score:4.54|P-value:1.47E-5 ID:SGTC_1404|Compound:3909-7820|FD-Score:-3.72|P-value:3.23E-4 ID:SGTC_1436|Compound:4092-1007|FD-Score:3.99|P-value:1.20E-4 ID:SGTC_1511|Compound:0906-3109|FD-Score:4.95|P-value:2.63E-6 ID:SGTC_1522|Compound:st012842|FD-Score:-3.72|P-value:3.26E-4 ID:SGTC_1572|Compound:epiandrosterone|FD-Score:3.89|P-value:1.70E-4 ID:SGTC_1573|Compound:oleylamine|FD-Score:4.00|P-value:1.15E-4 ID:SGTC_1588|Compound:farnesol|FD-Score:4.98|P-value:2.25E-6 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:5.25|P-value:6.89E-7 ID:SGTC_1666|Compound:st014829|FD-Score:4.80|P-value:4.95E-6 ID:SGTC_1712|Compound:st032288|FD-Score:4.30|P-value:3.72E-5 ID:SGTC_1725|Compound:st036284|FD-Score:3.52|P-value:5.89E-4 ID:SGTC_1729|Compound:st035615|FD-Score:4.93|P-value:2.85E-6 ID:SGTC_1730|Compound:st033808|FD-Score:4.84|P-value:4.20E-6 ID:SGTC_1841|Compound:st054148|FD-Score:5.33|P-value:4.60E-7 ID:SGTC_1855|Compound:st056195|FD-Score:-3.92|P-value:1.65E-4 ID:SGTC_1877|Compound:wortmannin|FD-Score:3.88|P-value:1.79E-4 ID:SGTC_1959|Compound:st077091|FD-Score:4.91|P-value:3.06E-6 ID:SGTC_1992|Compound:st073643|FD-Score:3.42|P-value:8.03E-4 ID:SGTC_2178|Compound:5812956|FD-Score:4.03|P-value:1.04E-4 ID:SGTC_2249|Compound:haloperidol|FD-Score:4.23|P-value:4.85E-5 ID:SGTC_2369|Compound:9071472|FD-Score:3.78|P-value:2.48E-4 ID:SGTC_2373|Compound:9071805|FD-Score:4.00|P-value:1.14E-4 ID:SGTC_2382|Compound:9035899|FD-Score:3.59|P-value:4.68E-4 ID:SGTC_2424|Compound:5537928|FD-Score:-3.96|P-value:1.41E-4 ID:SGTC_2436|Compound:5633522|FD-Score:-3.57|P-value:5.44E-4 ID:SGTC_2489|Compound:cantharidin|FD-Score:4.56|P-value:1.36E-5 ID:SGTC_2496|Compound:diffractaic acid|FD-Score:3.96|P-value:1.34E-4 ID:SGTC_2499|Compound:avocadenofuran|FD-Score:5.13|P-value:1.19E-6 ID:SGTC_2509|Compound:methyl 7-deshydroxypyrogallin-4-carboxylate|FD-Score:6.51|P-value:1.03E-9 ID:SGTC_2540|Compound:epiandrosterone|FD-Score:4.06|P-value:9.22E-5 ID:SGTC_2561|Compound:acetosyringone|FD-Score:3.96|P-value:1.34E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:-3.47|P-value:7.48E-4 ID:SGTC_2618|Compound:avocadyne|FD-Score:3.44|P-value:7.68E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:3.68|P-value:3.46E-4 ID:SGTC_2771|Compound:fluoxetine|FD-Score:4.70|P-value:7.66E-6 ID:SGTC_2921|Compound:7974240|FD-Score:3.84|P-value:2.01E-4 ID:SGTC_2950|Compound:9072895|FD-Score:7.01|P-value:5.65E-11 ID:SGTC_2953|Compound:9074162|FD-Score:-3.55|P-value:5.81E-4 ID:SGTC_2985|Compound:9022375|FD-Score:3.49|P-value:6.45E-4 ID:SGTC_3075|Compound:9116269|FD-Score:-4.77|P-value:6.24E-6 ID:SGTC_126|Compound:0417-1624|FD-Score:3.43|P-value:8.00E-4 ID:SGTC_176|Compound:1486-1328|FD-Score:4.59|P-value:1.18E-5 ID:SGTC_187|Compound:1488-2104|FD-Score:8.62|P-value:1.15E-15 ID:SGTC_241|Compound:5hr heat shock (37°C) + cantharidin|FD-Score:5.80|P-value:4.79E-8 ID:SGTC_262|Compound:fenpropimorph|FD-Score:4.15|P-value:6.70E-5 ID:SGTC_445|Compound:e6 berbamine|FD-Score:5.41|P-value:3.20E-7 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-7.90|P-value:2.08E-13 ID:SGTC_582|Compound:0987-0078|FD-Score:3.66|P-value:3.74E-4 ID:SGTC_649|Compound:1123-0311|FD-Score:3.91|P-value:1.60E-4 ID:SGTC_770|Compound:0389-1121|FD-Score:5.21|P-value:8.23E-7 ID:SGTC_831|Compound:k061-1017|FD-Score:3.81|P-value:2.25E-4 ID:SGTC_872|Compound:0588-0139|FD-Score:6.13|P-value:8.40E-9 ID:SGTC_1022|Compound:4477-0945|FD-Score:3.91|P-value:1.59E-4 ID:SGTC_1047|Compound:2043-3044|FD-Score:5.75|P-value:6.10E-8 ID:SGTC_1145|Compound:3546-0597|FD-Score:4.11|P-value:7.77E-5 ID:SGTC_1199|Compound:1638-0010|FD-Score:4.36|P-value:3.01E-5 ID:SGTC_1322|Compound:1348-1070|FD-Score:-3.54|P-value:5.94E-4 ID:SGTC_1330|Compound:1469-0054|FD-Score:7.51|P-value:2.41E-12 ID:SGTC_1335|Compound:1486-0900|FD-Score:5.80|P-value:4.72E-8 ID:SGTC_1402|Compound:3909-7673|FD-Score:4.54|P-value:1.47E-5 ID:SGTC_1404|Compound:3909-7820|FD-Score:-3.72|P-value:3.23E-4 ID:SGTC_1436|Compound:4092-1007|FD-Score:3.99|P-value:1.20E-4 ID:SGTC_1511|Compound:0906-3109|FD-Score:4.95|P-value:2.63E-6 ID:SGTC_1522|Compound:st012842|FD-Score:-3.72|P-value:3.26E-4 ID:SGTC_1572|Compound:epiandrosterone|FD-Score:3.89|P-value:1.70E-4 ID:SGTC_1573|Compound:oleylamine|FD-Score:4.00|P-value:1.15E-4 ID:SGTC_1588|Compound:farnesol|FD-Score:4.98|P-value:2.25E-6 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:5.25|P-value:6.89E-7 ID:SGTC_1666|Compound:st014829|FD-Score:4.80|P-value:4.95E-6 ID:SGTC_1712|Compound:st032288|FD-Score:4.30|P-value:3.72E-5 ID:SGTC_1725|Compound:st036284|FD-Score:3.52|P-value:5.89E-4 ID:SGTC_1729|Compound:st035615|FD-Score:4.93|P-value:2.85E-6 ID:SGTC_1730|Compound:st033808|FD-Score:4.84|P-value:4.20E-6 ID:SGTC_1841|Compound:st054148|FD-Score:5.33|P-value:4.60E-7 ID:SGTC_1855|Compound:st056195|FD-Score:-3.92|P-value:1.65E-4 ID:SGTC_1877|Compound:wortmannin|FD-Score:3.88|P-value:1.79E-4 ID:SGTC_1959|Compound:st077091|FD-Score:4.91|P-value:3.06E-6 ID:SGTC_1992|Compound:st073643|FD-Score:3.42|P-value:8.03E-4 ID:SGTC_2178|Compound:5812956|FD-Score:4.03|P-value:1.04E-4 ID:SGTC_2249|Compound:haloperidol|FD-Score:4.23|P-value:4.85E-5 ID:SGTC_2369|Compound:9071472|FD-Score:3.78|P-value:2.48E-4 ID:SGTC_2373|Compound:9071805|FD-Score:4.00|P-value:1.14E-4 ID:SGTC_2382|Compound:9035899|FD-Score:3.59|P-value:4.68E-4 ID:SGTC_2424|Compound:5537928|FD-Score:-3.96|P-value:1.41E-4 ID:SGTC_2436|Compound:5633522|FD-Score:-3.57|P-value:5.44E-4 ID:SGTC_2489|Compound:cantharidin|FD-Score:4.56|P-value:1.36E-5 ID:SGTC_2496|Compound:diffractaic acid|FD-Score:3.96|P-value:1.34E-4 ID:SGTC_2499|Compound:avocadenofuran|FD-Score:5.13|P-value:1.19E-6 ID:SGTC_2509|Compound:methyl 7-deshydroxypyrogallin-4-carboxylate|FD-Score:6.51|P-value:1.03E-9 ID:SGTC_2540|Compound:epiandrosterone|FD-Score:4.06|P-value:9.22E-5 ID:SGTC_2561|Compound:acetosyringone|FD-Score:3.96|P-value:1.34E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:-3.47|P-value:7.48E-4 ID:SGTC_2618|Compound:avocadyne|FD-Score:3.44|P-value:7.68E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:3.68|P-value:3.46E-4 ID:SGTC_2771|Compound:fluoxetine|FD-Score:4.70|P-value:7.66E-6 ID:SGTC_2921|Compound:7974240|FD-Score:3.84|P-value:2.01E-4 ID:SGTC_2950|Compound:9072895|FD-Score:7.01|P-value:5.65E-11 ID:SGTC_2953|Compound:9074162|FD-Score:-3.55|P-value:5.81E-4 ID:SGTC_2985|Compound:9022375|FD-Score:3.49|P-value:6.45E-4 ID:SGTC_3075|Compound:9116269|FD-Score:-4.77|P-value:6.24E-6

Top fitness defect scores for YNL025C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_187 1488-2104 8.62 32.7 µM 1.15E-15 calcium & mitochondrial duress 6 3.42E-18
2 SGTC_1330 1469-0054 7.51 61.8 µM 2.41E-12 iron homeostasis 1 2.88E-14
3 SGTC_2950 9072895 7.01 21.5 µM 5.65E-11 5 1.19E-12
4 SGTC_2509 methyl 7-deshydroxypyrogallin-4-carboxylate 6.51 54.6 µM 1.03E-9 RPP1 & pyrimidine depletion 7 3.66E-11
5 SGTC_872 0588-0139 6.13 234.0 µM 8.40E-9 7 4.33E-10
6 SGTC_1335 1486-0900 5.80 202.0 µM 4.72E-8 11 3.31E-9
7 SGTC_241 5hr heat shock (37°C) + cantharidin 5.80 155.4 µM 4.79E-8 cell wall signaling 35 3.36E-9
8 SGTC_1047 2043-3044 5.75 82.0 µM 6.10E-8 10 4.47E-9
9 SGTC_445 e6 berbamine 5.41 66.0 µM 3.20E-7 NEO1-PIK1 44 3.14E-8
10 SGTC_1841 st054148 5.33 70.0 µM 4.60E-7 2 4.82E-8
11 SGTC_1597 7-benzyloxygramine 5.25 47.5 µM 6.89E-7 calcium & mitochondrial duress 61 7.75E-8
12 SGTC_770 0389-1121 5.21 67.2 µM 8.23E-7 RPP1 & pyrimidine depletion 8 9.56E-8
13 SGTC_2499 avocadenofuran 5.13 51.5 µM 1.19E-6 14 1.47E-7
14 SGTC_1588 farnesol 4.98 29.9 µM 2.25E-6 2 3.12E-7
15 SGTC_1511 0906-3109 4.95 28.5 µM 2.63E-6 excess fatty acid 22 3.75E-7
16 SGTC_1729 st035615 4.93 46.0 µM 2.85E-6 18 4.12E-7
17 SGTC_1959 st077091 4.91 55.8 µM 3.06E-6 RPP1 & pyrimidine depletion 24 4.47E-7
18 SGTC_1730 st033808 4.84 45.1 µM 4.20E-6 13 6.50E-7
19 SGTC_1666 st014829 4.80 29.0 µM 4.95E-6 calcium & mitochondrial duress 17 7.88E-7
20 SGTC_2771 fluoxetine 4.70 41.0 µM 7.66E-6 10 1.32E-6

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.210 7.07E-35 YDR442W_d YDR442W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.187 1.12E-27 YDR443C SSN2 hom Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for stable association of Srb10p-Srb11p kinase; essential for transcriptional regulation
0.180 7.36E-26 YJL165C HAL5 hom Putative protein kinase; overexpression increases sodium and lithium tolerance, whereas gene disruption increases cation and low pH sensitivity and impairs potassium uptake, suggesting a role in regulation of Trk1p and/or Trk2p transporters; HAL5 has a paralog, KKQ8, that arose from the whole genome duplication
0.177 4.00E-25 YPL042C SSN3 hom Cyclin-dependent protein kinase, component of RNA polymerase II holoenzyme; involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression
0.175 1.24E-24 YIL105C SLM1 hom Phosphoinositide PI4,5P(2) binding protein, forms a complex with Slm2p; acts downstream of Mss4p in a pathway regulating actin cytoskeleton organization in response to stress; phosphorylated by the TORC2 complex; protein abundance increases in response to DNA replication stress; SLM1 has a paralog, SLM2, that arose from the whole genome duplication
0.158 4.17E-20 YGR285C ZUO1 hom Ribosome-associated chaperone, functions in ribosome biogenesis and, in partnership with Ssz1p and SSb1/2, as a chaperone for nascent polypeptide chains; contains a DnaJ domain and functions as a J-protein partner for Ssb1p and Ssb2p
0.153 4.32E-19 YHR209W CRG1 hom S-AdoMet-dependent methyltransferase involved in lipid homeostasis; mediates resistance to a drug cantharidin
0.149 3.98E-18 YPL145C KES1 hom One of seven members of the yeast oxysterol binding protein family; involved in negative regulation of Sec14p-dependent Golgi complex secretory functions, peripheral membrane protein that localizes to the Golgi complex; KES1 has a paralog, HES1, that arose from the whole genome duplication
0.145 2.42E-17 YNL288W CAF40 hom Evolutionarily conserved subunit of the CCR4-NOT complex involved in controlling mRNA initiation, elongation and degradation; binds Cdc39p
0.140 4.78E-16 YHR064C SSZ1 hom Hsp70 protein that interacts with Zuo1p (a DnaJ homolog) to form a ribosome-associated complex that binds the ribosome via the Zuo1p subunit; also involved in pleiotropic drug resistance via sequential activation of PDR1 and PDR5; binds ATP
0.130 3.96E-14 YGR159C NSR1 hom Nucleolar protein that binds nuclear localization sequences, required for pre-rRNA processing and ribosome biogenesis
0.128 1.17E-13 YFL033C RIM15 hom Glucose-repressible protein kinase involved in signal transduction during cell proliferation in response to nutrients, specifically the establishment of stationary phase; identified as a regulator of IME2; substrate of Pho80p-Pho85p kinase
0.124 6.07E-13 YJL129C TRK1 hom Component of the Trk1p-Trk2p potassium transport system; 180 kDa high affinity potassium transporter; phosphorylated in vivo and interacts physically with the phosphatase Ppz1p, suggesting Trk1p acitivy is regulated by phosphorylation; TRK1 has a paralog, TRK2, that arose from the whole genome duplication
0.119 4.31E-12 YMR099C YMR099C hom Glucose-6-phosphate 1-epimerase (hexose-6-phosphate mutarotase), likely involved in carbohydrate metabolism; GFP-fusion protein localizes to both the nucleus and cytoplasm and is induced in response to the DNA-damaging agent MMS
0.119 5.90E-12 YIL154C IMP2' hom Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat