HIPHOP chemogenomics database

YNL054W / VAC7

Integral vacuolar membrane protein involved in vacuole inheritance and morphology; activates Fab1p kinase activity under basal conditions and also after hyperosmotic shock

Zygosity: Homozygous strain

fixedexpanded
Profile for YNL054W / VAC7

Click on Significant Values for Screen Details

Top fitness defect scores for YNL054W deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_615	k087-0205	5.83	167.0 µM	1.48E-9		4	2.75E-9
2	SGTC_2509	methyl 7-deshydroxypyrogallin-4-carboxylate	5.77	54.6 µM	2.11E-9	RPP1 & pyrimidine depletion	9	3.93E-9
3	SGTC_2055	5235184	5.74	200.0 µM	2.50E-9	ERG2	9	4.64E-9
4	SGTC_868	0527-0199	5.43	213.0 µM	1.55E-8	fatty acid desaturase (OLE1)	9	2.86E-8
5	SGTC_882	0843-0131	4.97	351.0 µM	1.83E-7		5	3.32E-7
6	SGTC_264	promethazine	4.96	12.5 mM	1.96E-7	DNA intercalators	32	3.56E-7
7	SGTC_392	N-ethylmaleimide	4.88	8.3 µM	2.95E-7	RNA pol III & RNase P/MRP	5	5.34E-7
8	SGTC_1619	st003326	4.62	88.7 µM	1.07E-6	60S ribosome export	8	1.92E-6
9	SGTC_1919	st067113	4.48	52.3 µM	2.07E-6	TSC3-RPN4	20	3.67E-6
10	SGTC_2094	5332512	4.36	191.7 µM	3.63E-6		16	6.39E-6
11	SGTC_1511	0906-3109	4.32	28.5 µM	4.49E-6	excess fatty acid	34	7.89E-6
12	SGTC_1416	3966-0321	4.26	25.3 µM	5.94E-6		6	1.04E-5
13	SGTC_1082	camptothecin	4.19	424.9 nM	7.95E-6	DNA damage response	41	1.39E-5
14	SGTC_2006	4011487	4.15	200.0 µM	9.56E-6	endomembrane recycling	75	1.66E-5
15	SGTC_1081	sertraline	4.14	6.0 µM	9.95E-6	60S ribosome export	30	1.73E-5
16	SGTC_1744	st037937	4.10	38.9 µM	1.18E-5		26	2.05E-5
17	SGTC_2691	st077356	4.09	60.2 µM	1.25E-5		24	2.16E-5
18	SGTC_3208	9114115	3.96	49.5 µM	2.14E-5		34	3.68E-5
19	SGTC_1452	4194-0072	3.94	190.0 µM	2.33E-5		14	4.01E-5
20	SGTC_831	k061-1017	3.89	217.0 µM	2.94E-5	translation	67	5.03E-5

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.120	3.59E-12	YBR072W	HSP26	hom	Small heat shock protein (sHSP) with chaperone activity; forms hollow, sphere-shaped oligomers that suppress unfolded proteins aggregation; oligomer activation requires heat-induced conformational change; also has mRNA binding activity
0.107	5.91E-10	YLR291C	GCD7	het	Beta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression
0.093	7.70E-8	YBR152W	SPP381	het	mRNA splicing factor, component of U4/U6.U5 tri-snRNP; interacts genetically and physically with Prp38p
0.083	1.35E-6	YPL266W	DIM1	het	Essential 18S rRNA dimethylase (dimethyladenosine transferase), responsible for conserved m6(2)Am6(2)A dimethylation in 3'-terminal loop of 18S rRNA, part of 90S and 40S pre-particles in nucleolus, involved in pre-ribosomal RNA processing
0.079	4.49E-6	YNL227C	JJJ1	hom	Co-chaperone that stimulates the ATPase activity of Ssa1p, required for a late step of ribosome biogenesis; associated with the cytosolic large ribosomal subunit; contains a J-domain; mutation causes defects in fluid-phase endocytosis
0.079	4.60E-6	YMR113W	FOL3	het	Dihydrofolate synthetase, involved in folic acid biosynthesis; catalyzes the conversion of dihydropteroate to dihydrofolate in folate coenzyme biosynthesis
0.078	6.33E-6	YHR037W	PUT2	hom	Delta-1-pyrroline-5-carboxylate dehydrogenase, nuclear-encoded mitochondrial protein involved in utilization of proline as sole nitrogen source; deficiency of the human homolog causes HPII, an autosomal recessive inborn error of metabolism
0.076	9.91E-6	YPL143W	RPL33A	het	Ribosomal 60S subunit protein L33A; N-terminally acetylated; rpl33a null mutant exhibits slow growth while rpl33a rpl33b double null mutant is inviable; homologous to mammalian ribosomal protein L35A, no bacterial homolog; RPL33A has a paralog, RPL33B, that arose from the whole genome duplication
0.076	1.02E-5	YOR064C	YNG1	hom	Subunit of the NuA3 histone acetyltransferase complex that acetylates histone H3; contains PHD finger domain that interacts with methylated histone H3, has similarity to the human tumor suppressor ING1
0.071	3.83E-5	YER047C	SAP1	hom	Putative ATPase of the AAA family, interacts with the Sin1p transcriptional repressor in the two-hybrid system
0.070	5.51E-5	YMR313C	TGL3	hom	Bifunctional enzyme with triacylglycerol lipase and lysophosphatidylethanolamine acyltransferase activity; responsible for all the triacylglycerol lipase activity of the lipid particle; required with Tgl4p for timely bud formation
0.069	7.14E-5	YEL017W	GTT3	hom	Protein of unknown function with a possible role in glutathione metabolism, as suggested by computational analysis of large-scale protein-protein interaction data; GFP-fusion protein localizes to the nuclear periphery
0.067	9.73E-5	YOL064C	MET22	hom	Bisphosphate-3'-nucleotidase, involved in salt tolerance and methionine biogenesis; dephosphorylates 3'-phosphoadenosine-5'-phosphate and 3'-phosphoadenosine-5'-phosphosulfate, intermediates of the sulfate assimilation pathway
0.067	1.01E-4	YNL170W_d	YNL170W_d	hom	Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.066	1.29E-4	YOR260W	GCD1	het	Gamma subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression