YNL116W / DMA2

Ubiquitin-protein ligase (E3); controls septin dynamics and the spindle position checkpoint (SPOC) along with functionally redundant ligase Dma1p by regulating the recruitment of Elm1p to the bud neck; regulates levels of the translation initiation factor eIF2 subunit Gcd11p, as well as abundance, localization, and ubiquitination of Cdk inhibitory kinase Swe1p; ortholog of human RNF8 protein, with sequence similarity to human Chfr; contains FHA and RING finger domains

Zygosity: Homozygous strain
fixedexpanded
Profile for YNL116W / DMA2

Click on Significant Values for Screen Details ID:SGTC_133|Compound:0724-0033|FD-Score:-3.38|P-value:5.93E-4 ID:SGTC_261|Compound:5-fluorouridine|FD-Score:6.10|P-value:9.00E-9 ID:SGTC_307|Compound:1501-1223|FD-Score:4.57|P-value:1.36E-5 ID:SGTC_449|Compound:xestospongin c|FD-Score:4.05|P-value:1.03E-4 ID:SGTC_516|Compound:3992-2719|FD-Score:-3.95|P-value:8.26E-5 ID:SGTC_616|Compound:4092-0450|FD-Score:-3.86|P-value:1.14E-4 ID:SGTC_774|Compound:k060-0086|FD-Score:3.51|P-value:6.75E-4 ID:SGTC_807|Compound:1315-0151|FD-Score:-4.38|P-value:1.50E-5 ID:SGTC_811|Compound:0416-0114|FD-Score:7.13|P-value:2.13E-11 ID:SGTC_937|Compound:3122-0005|FD-Score:-3.41|P-value:5.46E-4 ID:SGTC_943|Compound:3253-1379|FD-Score:-3.63|P-value:2.58E-4 ID:SGTC_1041|Compound:0206-0046|FD-Score:5.00|P-value:2.10E-6 ID:SGTC_1375|Compound:2910-0797|FD-Score:3.41|P-value:9.32E-4 ID:SGTC_1391|Compound:3442-0225|FD-Score:3.69|P-value:3.65E-4 ID:SGTC_1579|Compound:chlormadinone acetate|FD-Score:-3.68|P-value:2.18E-4 ID:SGTC_1586|Compound:deoxycorticosterone acetate|FD-Score:3.63|P-value:4.54E-4 ID:SGTC_1852|Compound:st056254|FD-Score:3.52|P-value:6.60E-4 ID:SGTC_1878|Compound:temozolomide|FD-Score:3.41|P-value:9.20E-4 ID:SGTC_1948|Compound:st076329|FD-Score:-4.11|P-value:4.40E-5 ID:SGTC_1986|Compound:st071812|FD-Score:4.09|P-value:8.93E-5 ID:SGTC_1998|Compound:st075797|FD-Score:3.41|P-value:9.35E-4 ID:SGTC_1999|Compound:st074831|FD-Score:3.55|P-value:5.84E-4 ID:SGTC_2064|Compound:5220758|FD-Score:3.60|P-value:4.99E-4 ID:SGTC_2575|Compound:plumbagin|FD-Score:4.69|P-value:8.03E-6 ID:SGTC_2626|Compound:ethyl orsellinate|FD-Score:3.68|P-value:3.87E-4 ID:SGTC_2632|Compound:3-hydroxy-4-(succin-2-yl)-caryolane delta-lactone|FD-Score:4.66|P-value:9.44E-6 ID:SGTC_2639|Compound:lactacystin|FD-Score:-3.65|P-value:2.43E-4 ID:SGTC_2818|Compound:7996756|FD-Score:3.91|P-value:1.70E-4 ID:SGTC_2995|Compound:9067767|FD-Score:-3.44|P-value:4.98E-4 ID:SGTC_3022|Compound:9087993|FD-Score:3.48|P-value:7.41E-4 ID:SGTC_133|Compound:0724-0033|FD-Score:-3.38|P-value:5.93E-4 ID:SGTC_261|Compound:5-fluorouridine|FD-Score:6.10|P-value:9.00E-9 ID:SGTC_307|Compound:1501-1223|FD-Score:4.57|P-value:1.36E-5 ID:SGTC_449|Compound:xestospongin c|FD-Score:4.05|P-value:1.03E-4 ID:SGTC_516|Compound:3992-2719|FD-Score:-3.95|P-value:8.26E-5 ID:SGTC_616|Compound:4092-0450|FD-Score:-3.86|P-value:1.14E-4 ID:SGTC_774|Compound:k060-0086|FD-Score:3.51|P-value:6.75E-4 ID:SGTC_807|Compound:1315-0151|FD-Score:-4.38|P-value:1.50E-5 ID:SGTC_811|Compound:0416-0114|FD-Score:7.13|P-value:2.13E-11 ID:SGTC_937|Compound:3122-0005|FD-Score:-3.41|P-value:5.46E-4 ID:SGTC_943|Compound:3253-1379|FD-Score:-3.63|P-value:2.58E-4 ID:SGTC_1041|Compound:0206-0046|FD-Score:5.00|P-value:2.10E-6 ID:SGTC_1375|Compound:2910-0797|FD-Score:3.41|P-value:9.32E-4 ID:SGTC_1391|Compound:3442-0225|FD-Score:3.69|P-value:3.65E-4 ID:SGTC_1579|Compound:chlormadinone acetate|FD-Score:-3.68|P-value:2.18E-4 ID:SGTC_1586|Compound:deoxycorticosterone acetate|FD-Score:3.63|P-value:4.54E-4 ID:SGTC_1852|Compound:st056254|FD-Score:3.52|P-value:6.60E-4 ID:SGTC_1878|Compound:temozolomide|FD-Score:3.41|P-value:9.20E-4 ID:SGTC_1948|Compound:st076329|FD-Score:-4.11|P-value:4.40E-5 ID:SGTC_1986|Compound:st071812|FD-Score:4.09|P-value:8.93E-5 ID:SGTC_1998|Compound:st075797|FD-Score:3.41|P-value:9.35E-4 ID:SGTC_1999|Compound:st074831|FD-Score:3.55|P-value:5.84E-4 ID:SGTC_2064|Compound:5220758|FD-Score:3.60|P-value:4.99E-4 ID:SGTC_2575|Compound:plumbagin|FD-Score:4.69|P-value:8.03E-6 ID:SGTC_2626|Compound:ethyl orsellinate|FD-Score:3.68|P-value:3.87E-4 ID:SGTC_2632|Compound:3-hydroxy-4-(succin-2-yl)-caryolane delta-lactone|FD-Score:4.66|P-value:9.44E-6 ID:SGTC_2639|Compound:lactacystin|FD-Score:-3.65|P-value:2.43E-4 ID:SGTC_2818|Compound:7996756|FD-Score:3.91|P-value:1.70E-4 ID:SGTC_2995|Compound:9067767|FD-Score:-3.44|P-value:4.98E-4 ID:SGTC_3022|Compound:9087993|FD-Score:3.48|P-value:7.41E-4

Top fitness defect scores for YNL116W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_811 0416-0114 7.13 265.0 µM 2.13E-11 1 4.94E-13
2 SGTC_261 5-fluorouridine 6.10 15.4 µM 9.00E-9 exosome 3 5.27E-10
3 SGTC_1041 0206-0046 5.00 107.0 µM 2.10E-6 14 2.85E-7
4 SGTC_2575 plumbagin 4.69 510.0 nM 8.03E-6 superoxide 24 1.34E-6
5 SGTC_2632 3-hydroxy-4-(succin-2-yl)-caryolane delta-lactone 4.66 100.0 µM 9.44E-6 10 1.62E-6
6 SGTC_307 1501-1223 4.57 22.3 µM 1.36E-5 5 2.48E-6
7 SGTC_1986 st071812 4.09 45.0 µM 8.93E-5 4 2.17E-5
8 SGTC_449 xestospongin c 4.05 11.2 µM 1.03E-4 NEO1-PIK1 67 2.55E-5
9 SGTC_2818 7996756 3.91 13.0 µM 1.70E-4 18 4.57E-5
10 SGTC_1391 3442-0225 3.69 151.0 µM 3.65E-4 21 1.11E-4
11 SGTC_2626 ethyl orsellinate 3.68 100.0 µM 3.87E-4 41 1.18E-4
12 SGTC_1586 deoxycorticosterone acetate 3.63 53.7 µM 4.54E-4 19 1.43E-4
13 SGTC_2064 5220758 3.60 200.0 µM 4.99E-4 46 1.59E-4
14 SGTC_1999 st074831 3.55 12.5 µM 5.84E-4 plasma membrane duress 46 1.90E-4
15 SGTC_1852 st056254 3.52 31.4 µM 6.60E-4 19 2.20E-4
16 SGTC_774 k060-0086 3.51 136.0 µM 6.75E-4 34 2.25E-4
17 SGTC_3022 9087993 3.48 49.5 µM 7.41E-4 15 2.51E-4
18 SGTC_1878 temozolomide 3.41 1.0 mM 9.20E-4 19 3.22E-4
19 SGTC_1375 2910-0797 3.41 186.0 µM 9.32E-4 45 3.27E-4
20 SGTC_1998 st075797 3.41 76.8 µM 9.35E-4 mitochondrial response to ROS 31 3.29E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.088 3.03E-7 YMR115W MGR3 hom Subunit of the mitochondrial (mt) i-AAA protease supercomplex, which degrades misfolded mitochondrial proteins; forms a subcomplex with Mgr1p that binds to substrates to facilitate proteolysis; required for growth of cells lacking mtDNA
0.087 4.15E-7 YIL063C YRB2 het Protein of unknown function involved in nuclear processes of the Ran-GTPase cycle; involved in nuclear protein export; contains Ran Binding Domain and FxFG repeats; interacts with Srm1p, GTP-Gsp1p, Rna1p and Crm1p; is not essential
0.086 6.47E-7 YNR050C LYS9 hom Saccharopine dehydrogenase (NADP+, L-glutamate-forming); catalyzes the formation of saccharopine from alpha-aminoadipate 6-semialdehyde, the seventh step in lysine biosynthesis pathway; exhibits genetic and physical interactions with TRM112
0.082 2.25E-6 YHR108W GGA2 hom Protein that interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner to facilitate traffic through the late Golgi; binds phosphatidylinositol 4-phosphate, which plays a role in TGN localization; has homology to gamma-adaptin
0.080 3.52E-6 YGR235C MOS2 hom Mitochondrial inner membrane protein; non-essential component of the mitochondrial inner membrane organizing system (MINOS, MitOS, or MICOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture
0.075 1.31E-5 YBR197C YBR197C hom Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR197C is not an essential gene; relative distribution to the nucleus increases upon DNA replication stress; YBR197C has a paralog, YPL077C, that arose from the whole genome duplication
0.075 1.33E-5 YOL057W YOL057W hom Dipeptidyl-peptidase III; cleaves dipeptides from the amino terminus of target proteins; highly active on synthetic substrate Arg-Arg-2-naphthylamide; mammalian ortholog may be a biomarker for some cancers
0.073 2.44E-5 YAL042W ERV46 hom Protein localized to COPII-coated vesicles, forms a complex with Erv41p; involved in the membrane fusion stage of transport
0.070 5.44E-5 YJL136W-A_p YJL136W-A_p hom Putative protein of unknown function; identified by SAGE
0.069 6.00E-5 YCR102C_p YCR102C_p hom Putative protein of unknown function, involved in copper metabolism; similar to C. carbonum toxD gene; member of the quinone oxidoreductase family
0.069 6.07E-5 YMR175W-A_p YMR175W-A_p hom Putative protein of unknown function
0.067 9.90E-5 YJL214W HXT8 hom Protein of unknown function with similarity to hexose transporter family members, expression is induced by low levels of glucose and repressed by high levels of glucose
0.067 9.93E-5 YPR016C TIF6 het Constituent of 66S pre-ribosomal particles, has similarity to human translation initiation factor 6 (eIF6); may be involved in the biogenesis and or stability of 60S ribosomal subunits
0.067 1.08E-4 YJL047C-A_p YJL047C-A_p hom Putative protein of unknown function
0.066 1.29E-4 YNR013C PHO91 hom Low-affinity phosphate transporter of the vacuolar membrane; deletion of pho84, pho87, pho89, pho90, and pho91 causes synthetic lethality; transcription independent of Pi and Pho4p activity; overexpression results in vigorous growth