YNL117W / MLS1

Malate synthase, enzyme of the glyoxylate cycle; involved in utilization of non-fermentable carbon sources; expression is subject to carbon catabolite repression; localizes in peroxisomes during growth on oleic acid, otherwise cytosolic

Zygosity: Homozygous strain
fixedexpanded
Profile for YNL117W / MLS1

Click on Significant Values for Screen Details ID:SGTC_109|Compound:0132-0036|FD-Score:-3.35|P-value:5.67E-4 ID:SGTC_189|Compound:3970-0742|FD-Score:3.26|P-value:8.46E-4 ID:SGTC_278|Compound:4092-0821|FD-Score:-3.21|P-value:9.18E-4 ID:SGTC_446|Compound:go6976|FD-Score:-3.89|P-value:7.98E-5 ID:SGTC_462|Compound:ag-879|FD-Score:3.79|P-value:1.29E-4 ID:SGTC_579|Compound:4491-1009|FD-Score:3.83|P-value:1.11E-4 ID:SGTC_816|Compound:0325-0417|FD-Score:-3.48|P-value:3.66E-4 ID:SGTC_819|Compound:1013-0258|FD-Score:3.85|P-value:1.02E-4 ID:SGTC_822|Compound:1487-0950|FD-Score:-3.28|P-value:7.24E-4 ID:SGTC_824|Compound:1304-0008|FD-Score:3.39|P-value:5.50E-4 ID:SGTC_828|Compound:2074-0507|FD-Score:-3.90|P-value:7.85E-5 ID:SGTC_1045|Compound:1348-1485|FD-Score:4.36|P-value:1.34E-5 ID:SGTC_1046|Compound:2001-0062|FD-Score:3.67|P-value:2.00E-4 ID:SGTC_1077|Compound:5-fluorocytosine|FD-Score:3.67|P-value:2.02E-4 ID:SGTC_1149|Compound:3909-7961|FD-Score:3.67|P-value:2.03E-4 ID:SGTC_1281|Compound:0884-0031|FD-Score:3.54|P-value:3.19E-4 ID:SGTC_1286|Compound:0929-0070|FD-Score:3.47|P-value:4.19E-4 ID:SGTC_1323|Compound:1348-1351|FD-Score:3.43|P-value:4.72E-4 ID:SGTC_1342|Compound:1486-1464|FD-Score:3.29|P-value:7.63E-4 ID:SGTC_1607|Compound:st000305|FD-Score:3.36|P-value:6.09E-4 ID:SGTC_1644|Compound:st010134|FD-Score:-3.33|P-value:6.20E-4 ID:SGTC_1645|Compound:Cervolide|FD-Score:4.33|P-value:1.52E-5 ID:SGTC_1664|Compound:st014217|FD-Score:4.63|P-value:3.98E-6 ID:SGTC_1815|Compound:st051655|FD-Score:3.37|P-value:5.78E-4 ID:SGTC_2051|Compound:5263410|FD-Score:3.68|P-value:1.92E-4 ID:SGTC_2058|Compound:5236698|FD-Score:5.70|P-value:1.90E-8 ID:SGTC_2128|Compound:5255449|FD-Score:-3.26|P-value:7.70E-4 ID:SGTC_2186|Compound:6124449|FD-Score:4.50|P-value:7.16E-6 ID:SGTC_2323|Compound:6148992|FD-Score:3.61|P-value:2.52E-4 ID:SGTC_2487|Compound:5918373|FD-Score:3.40|P-value:5.21E-4 ID:SGTC_2911|Compound:7942208|FD-Score:5.10|P-value:4.29E-7 ID:SGTC_109|Compound:0132-0036|FD-Score:-3.35|P-value:5.67E-4 ID:SGTC_189|Compound:3970-0742|FD-Score:3.26|P-value:8.46E-4 ID:SGTC_278|Compound:4092-0821|FD-Score:-3.21|P-value:9.18E-4 ID:SGTC_446|Compound:go6976|FD-Score:-3.89|P-value:7.98E-5 ID:SGTC_462|Compound:ag-879|FD-Score:3.79|P-value:1.29E-4 ID:SGTC_579|Compound:4491-1009|FD-Score:3.83|P-value:1.11E-4 ID:SGTC_816|Compound:0325-0417|FD-Score:-3.48|P-value:3.66E-4 ID:SGTC_819|Compound:1013-0258|FD-Score:3.85|P-value:1.02E-4 ID:SGTC_822|Compound:1487-0950|FD-Score:-3.28|P-value:7.24E-4 ID:SGTC_824|Compound:1304-0008|FD-Score:3.39|P-value:5.50E-4 ID:SGTC_828|Compound:2074-0507|FD-Score:-3.90|P-value:7.85E-5 ID:SGTC_1045|Compound:1348-1485|FD-Score:4.36|P-value:1.34E-5 ID:SGTC_1046|Compound:2001-0062|FD-Score:3.67|P-value:2.00E-4 ID:SGTC_1077|Compound:5-fluorocytosine|FD-Score:3.67|P-value:2.02E-4 ID:SGTC_1149|Compound:3909-7961|FD-Score:3.67|P-value:2.03E-4 ID:SGTC_1281|Compound:0884-0031|FD-Score:3.54|P-value:3.19E-4 ID:SGTC_1286|Compound:0929-0070|FD-Score:3.47|P-value:4.19E-4 ID:SGTC_1323|Compound:1348-1351|FD-Score:3.43|P-value:4.72E-4 ID:SGTC_1342|Compound:1486-1464|FD-Score:3.29|P-value:7.63E-4 ID:SGTC_1607|Compound:st000305|FD-Score:3.36|P-value:6.09E-4 ID:SGTC_1644|Compound:st010134|FD-Score:-3.33|P-value:6.20E-4 ID:SGTC_1645|Compound:Cervolide|FD-Score:4.33|P-value:1.52E-5 ID:SGTC_1664|Compound:st014217|FD-Score:4.63|P-value:3.98E-6 ID:SGTC_1815|Compound:st051655|FD-Score:3.37|P-value:5.78E-4 ID:SGTC_2051|Compound:5263410|FD-Score:3.68|P-value:1.92E-4 ID:SGTC_2058|Compound:5236698|FD-Score:5.70|P-value:1.90E-8 ID:SGTC_2128|Compound:5255449|FD-Score:-3.26|P-value:7.70E-4 ID:SGTC_2186|Compound:6124449|FD-Score:4.50|P-value:7.16E-6 ID:SGTC_2323|Compound:6148992|FD-Score:3.61|P-value:2.52E-4 ID:SGTC_2487|Compound:5918373|FD-Score:3.40|P-value:5.21E-4 ID:SGTC_2911|Compound:7942208|FD-Score:5.10|P-value:4.29E-7

Top fitness defect scores for YNL117W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2058 5236698 5.70 133.0 µM 1.90E-8 10 5.99E-9
2 SGTC_2911 7942208 5.10 15.6 µM 4.29E-7 mitochondrial response to ROS 5 1.68E-7
3 SGTC_1664 st014217 4.63 9.0 µM 3.98E-6 60S ribosome export 5 1.82E-6
4 SGTC_2186 6124449 4.50 200.0 µM 7.16E-6 RSC complex & mRNA processing 14 3.41E-6
5 SGTC_1045 1348-1485 4.36 165.0 µM 1.34E-5 18 6.64E-6
6 SGTC_1645 Cervolide 4.33 78.0 µM 1.52E-5 3 7.61E-6
7 SGTC_819 1013-0258 3.85 27.7 µM 1.02E-4 38 5.81E-5
8 SGTC_579 4491-1009 3.83 110.0 µM 1.11E-4 60S ribosome export 25 6.36E-5
9 SGTC_462 ag-879 3.79 158.0 µM 1.29E-4 36 7.47E-5
10 SGTC_2051 5263410 3.68 176.0 µM 1.92E-4 21 1.14E-4
11 SGTC_1046 2001-0062 3.67 216.0 µM 2.00E-4 27 1.20E-4
12 SGTC_1077 5-fluorocytosine 3.67 377.0 nM 2.02E-4 exosome 22 1.21E-4
13 SGTC_1149 3909-7961 3.67 23.3 µM 2.03E-4 29 1.22E-4
14 SGTC_2323 6148992 3.61 148.3 µM 2.52E-4 46 1.53E-4
15 SGTC_1281 0884-0031 3.54 11.8 µM 3.19E-4 fatty acid desaturase (OLE1) 33 1.97E-4
16 SGTC_1286 0929-0070 3.47 131.0 µM 4.19E-4 33 2.64E-4
17 SGTC_1323 1348-1351 3.43 11.2 µM 4.72E-4 18 3.00E-4
18 SGTC_2487 5918373 3.40 44.0 µM 5.21E-4 33 3.33E-4
19 SGTC_824 1304-0008 3.39 100.0 µM 5.50E-4 DNA damage response 56 3.53E-4
20 SGTC_1815 st051655 3.37 58.2 µM 5.78E-4 TSC3-RPN4 16 3.72E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.111 1.33E-10 YOR233W KIN4 hom Serine/threonine protein kinase; inhibits the mitotic exit network (MEN) when the spindle position checkpoint is activated; localized asymmetrically to mother cell cortex, spindle pole body and bud neck; KIN4 has a paralog, FRK1, that arose from the whole genome duplication
0.095 3.94E-8 YER068C-A_d YER068C-A_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.092 8.52E-8 YHR024C MAS2 het Larger subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins
0.083 1.40E-6 YPL001W HAT1 hom Catalytic subunit of the Hat1p-Hat2p histone acetyltransferase complex that uses the cofactor acetyl coenzyme A, to acetylate free nuclear and cytoplasmic histone H4; involved in telomeric silencing and DNA double-strand break repair
0.082 2.04E-6 YGR092W DBF2 hom Ser/Thr kinase involved in transcription and stress response; functions as part of a network of genes in exit from mitosis; localization is cell cycle regulated; activated by Cdc15p during the exit from mitosis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs; phosphorylates Chs2p to regulate primary septum formation and Hof1p to regulate cytokinesis
0.082 2.04E-6 YER186C_p YER186C_p hom Putative protein of unknown function
0.076 1.12E-5 YJR050W ISY1 hom Member of NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of spliceosome containing U2, U5, and U6 snRNAs, interacts with Prp16p to modulate splicing fidelity; isy1 syf2 cells have defective spindles
0.075 1.29E-5 YDL015C TSC13 het Enoyl reductase; catalyzes the last step in each cycle of very long chain fatty acid elongation; localizes to the ER, highly enriched in a structure marking nuclear-vacuolar junctions; coimmunoprecipitates with elongases Fen1p and Sur4p; protein increases in abundance and relative distribution to ER foci increases upon DNA replication stress
0.073 2.48E-5 YLR113W HOG1 hom Mitogen-activated protein kinase involved in osmoregulation; controls global reallocation of RNA Pol II in response to osmotic shock; acts via three independent osmosensors; mitophagy-specific regulator; mediates the recruitment and activation of RNA Pol II at Hot1p-dependent promoters; localization regulated by Ptp2p and Ptp3p; protein abundance increases in response to DNA replication stress
0.071 3.41E-5 YDL111C RRP42 het Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp42p (EXOSC7)
0.071 3.62E-5 YOL073C YOL073C hom Subunit of the DSC ubiquitin ligase complex; ortholog of fission yeast dsc2
0.071 3.91E-5 YLR432W IMD3 hom Inosine monophosphate dehydrogenase, catalyzes the first step of GMP biosynthesis, member of a four-gene family in S. cerevisiae, constitutively expressed
0.070 5.11E-5 YPR082C DIB1 het 17-kDa component of the U4/U6aU5 tri-snRNP, plays an essential role in pre-mRNA splicing, orthologue of hDIM1, the human U5-specific 15-kDa protein
0.069 6.35E-5 YGL169W SUA5 het Single-stranded telomeric DNA-binding protein, required for normal telomere length; null mutant lacks N6-threonylcarbamoyl adenosine (t6A) modification in the anticodon loop of ANN-decoding tRNA; member of conserved YrdC/Sua5 family
0.069 6.28E-5 YMR121C RPL15B hom Ribosomal 60S subunit protein L15B; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15B has a paralog, RPL15A, that arose from the whole genome duplication; relocalizes from nucleus to nucleolus upon DNA replication stress