YNL119W / NCS2

Protein required for thiolation of the uridine at the wobble position of Lys(UUU) and Glu(UUC) tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae

Zygosity: Homozygous strain
fixedexpanded
Profile for YNL119W / NCS2

Click on Significant Values for Screen Details ID:SGTC_83|Compound:0170-0301|FD-Score:-3.31|P-value:8.00E-4 ID:SGTC_95|Compound:0471-0001|FD-Score:-3.46|P-value:4.65E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:3.40|P-value:4.00E-4 ID:SGTC_320|Compound:4092-0846|FD-Score:-4.16|P-value:3.37E-5 ID:SGTC_423|Compound:5-fluorouracil|FD-Score:3.17|P-value:8.70E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-3.88|P-value:1.02E-4 ID:SGTC_467|Compound:ro 31-8220|FD-Score:3.71|P-value:1.28E-4 ID:SGTC_511|Compound:blebbistatin|FD-Score:4.02|P-value:3.79E-5 ID:SGTC_567|Compound:1080-0542|FD-Score:4.68|P-value:2.14E-6 ID:SGTC_761|Compound:k216-0224|FD-Score:3.66|P-value:1.56E-4 ID:SGTC_804|Compound:1595-0054|FD-Score:-4.44|P-value:1.02E-5 ID:SGTC_806|Compound:3966-4455|FD-Score:-4.27|P-value:2.09E-5 ID:SGTC_808|Compound:0711-0032|FD-Score:3.71|P-value:1.25E-4 ID:SGTC_941|Compound:3237-0776|FD-Score:4.35|P-value:9.16E-6 ID:SGTC_957|Compound:1187-1561|FD-Score:3.25|P-value:6.49E-4 ID:SGTC_1038|Compound:k292-0204|FD-Score:3.73|P-value:1.18E-4 ID:SGTC_1106|Compound:tegaserod maleate|FD-Score:3.22|P-value:7.22E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:3.72|P-value:1.22E-4 ID:SGTC_1208|Compound:2323-2347|FD-Score:3.64|P-value:1.65E-4 ID:SGTC_1249|Compound:0481-0015|FD-Score:3.17|P-value:8.52E-4 ID:SGTC_1447|Compound:4239-0816|FD-Score:-4.69|P-value:3.38E-6 ID:SGTC_1448|Compound:4255-0083|FD-Score:-3.31|P-value:7.85E-4 ID:SGTC_1520|Compound:flindersine|FD-Score:-3.79|P-value:1.43E-4 ID:SGTC_1718|Compound:roemerine|FD-Score:-3.70|P-value:2.02E-4 ID:SGTC_1748|Compound:st037455|FD-Score:4.32|P-value:1.08E-5 ID:SGTC_1844|Compound:rapamycin|FD-Score:14.50|P-value:1.62E-45 ID:SGTC_1852|Compound:st056254|FD-Score:-3.47|P-value:4.50E-4 ID:SGTC_1899|Compound:st063254|FD-Score:4.90|P-value:7.23E-7 ID:SGTC_2093|Compound:5331342|FD-Score:3.66|P-value:1.55E-4 ID:SGTC_2127|Compound:5253358|FD-Score:4.11|P-value:2.55E-5 ID:SGTC_2132|Compound:5317594|FD-Score:-4.98|P-value:8.69E-7 ID:SGTC_2202|Compound:7226349|FD-Score:-3.36|P-value:6.59E-4 ID:SGTC_2358|Compound:9037157|FD-Score:6.08|P-value:1.28E-9 ID:SGTC_2361|Compound:9034121|FD-Score:3.41|P-value:3.80E-4 ID:SGTC_2617|Compound:dihydrotanshinone i|FD-Score:3.17|P-value:8.68E-4 ID:SGTC_2679|Compound:rapamycin|FD-Score:12.30|P-value:1.34E-33 ID:SGTC_2688|Compound:kanamycin b|FD-Score:5.92|P-value:3.21E-9 ID:SGTC_2778|Compound:tioconazole|FD-Score:-4.08|P-value:4.70E-5 ID:SGTC_2804|Compound:7954240|FD-Score:3.19|P-value:8.04E-4 ID:SGTC_2868|Compound:9035608|FD-Score:-3.32|P-value:7.61E-4 ID:SGTC_2877|Compound:9040724|FD-Score:-4.50|P-value:7.89E-6 ID:SGTC_2896|Compound:9052656|FD-Score:-5.34|P-value:1.42E-7 ID:SGTC_2930|Compound:9013230|FD-Score:-4.23|P-value:2.50E-5 ID:SGTC_2933|Compound:9026794|FD-Score:3.53|P-value:2.44E-4 ID:SGTC_2938|Compound:9059746|FD-Score:-6.01|P-value:3.75E-9 ID:SGTC_2939|Compound:9044578|FD-Score:-8.36|P-value:3.51E-16 ID:SGTC_2949|Compound:9072542|FD-Score:3.76|P-value:1.04E-4 ID:SGTC_2997|Compound:9067075|FD-Score:-5.29|P-value:1.89E-7 ID:SGTC_3138|Compound:9097216|FD-Score:3.18|P-value:8.48E-4 ID:SGTC_3141|Compound:9094609|FD-Score:-3.92|P-value:8.60E-5 ID:SGTC_3216|Compound:9129716|FD-Score:3.84|P-value:7.85E-5 ID:SGTC_3298|Compound:9116858|FD-Score:-3.27|P-value:9.02E-4 ID:SGTC_3330|Compound:9141424|FD-Score:3.33|P-value:4.96E-4 ID:SGTC_83|Compound:0170-0301|FD-Score:-3.31|P-value:8.00E-4 ID:SGTC_95|Compound:0471-0001|FD-Score:-3.46|P-value:4.65E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:3.40|P-value:4.00E-4 ID:SGTC_320|Compound:4092-0846|FD-Score:-4.16|P-value:3.37E-5 ID:SGTC_423|Compound:5-fluorouracil|FD-Score:3.17|P-value:8.70E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-3.88|P-value:1.02E-4 ID:SGTC_467|Compound:ro 31-8220|FD-Score:3.71|P-value:1.28E-4 ID:SGTC_511|Compound:blebbistatin|FD-Score:4.02|P-value:3.79E-5 ID:SGTC_567|Compound:1080-0542|FD-Score:4.68|P-value:2.14E-6 ID:SGTC_761|Compound:k216-0224|FD-Score:3.66|P-value:1.56E-4 ID:SGTC_804|Compound:1595-0054|FD-Score:-4.44|P-value:1.02E-5 ID:SGTC_806|Compound:3966-4455|FD-Score:-4.27|P-value:2.09E-5 ID:SGTC_808|Compound:0711-0032|FD-Score:3.71|P-value:1.25E-4 ID:SGTC_941|Compound:3237-0776|FD-Score:4.35|P-value:9.16E-6 ID:SGTC_957|Compound:1187-1561|FD-Score:3.25|P-value:6.49E-4 ID:SGTC_1038|Compound:k292-0204|FD-Score:3.73|P-value:1.18E-4 ID:SGTC_1106|Compound:tegaserod maleate|FD-Score:3.22|P-value:7.22E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:3.72|P-value:1.22E-4 ID:SGTC_1208|Compound:2323-2347|FD-Score:3.64|P-value:1.65E-4 ID:SGTC_1249|Compound:0481-0015|FD-Score:3.17|P-value:8.52E-4 ID:SGTC_1447|Compound:4239-0816|FD-Score:-4.69|P-value:3.38E-6 ID:SGTC_1448|Compound:4255-0083|FD-Score:-3.31|P-value:7.85E-4 ID:SGTC_1520|Compound:flindersine|FD-Score:-3.79|P-value:1.43E-4 ID:SGTC_1718|Compound:roemerine|FD-Score:-3.70|P-value:2.02E-4 ID:SGTC_1748|Compound:st037455|FD-Score:4.32|P-value:1.08E-5 ID:SGTC_1844|Compound:rapamycin|FD-Score:14.50|P-value:1.62E-45 ID:SGTC_1852|Compound:st056254|FD-Score:-3.47|P-value:4.50E-4 ID:SGTC_1899|Compound:st063254|FD-Score:4.90|P-value:7.23E-7 ID:SGTC_2093|Compound:5331342|FD-Score:3.66|P-value:1.55E-4 ID:SGTC_2127|Compound:5253358|FD-Score:4.11|P-value:2.55E-5 ID:SGTC_2132|Compound:5317594|FD-Score:-4.98|P-value:8.69E-7 ID:SGTC_2202|Compound:7226349|FD-Score:-3.36|P-value:6.59E-4 ID:SGTC_2358|Compound:9037157|FD-Score:6.08|P-value:1.28E-9 ID:SGTC_2361|Compound:9034121|FD-Score:3.41|P-value:3.80E-4 ID:SGTC_2617|Compound:dihydrotanshinone i|FD-Score:3.17|P-value:8.68E-4 ID:SGTC_2679|Compound:rapamycin|FD-Score:12.30|P-value:1.34E-33 ID:SGTC_2688|Compound:kanamycin b|FD-Score:5.92|P-value:3.21E-9 ID:SGTC_2778|Compound:tioconazole|FD-Score:-4.08|P-value:4.70E-5 ID:SGTC_2804|Compound:7954240|FD-Score:3.19|P-value:8.04E-4 ID:SGTC_2868|Compound:9035608|FD-Score:-3.32|P-value:7.61E-4 ID:SGTC_2877|Compound:9040724|FD-Score:-4.50|P-value:7.89E-6 ID:SGTC_2896|Compound:9052656|FD-Score:-5.34|P-value:1.42E-7 ID:SGTC_2930|Compound:9013230|FD-Score:-4.23|P-value:2.50E-5 ID:SGTC_2933|Compound:9026794|FD-Score:3.53|P-value:2.44E-4 ID:SGTC_2938|Compound:9059746|FD-Score:-6.01|P-value:3.75E-9 ID:SGTC_2939|Compound:9044578|FD-Score:-8.36|P-value:3.51E-16 ID:SGTC_2949|Compound:9072542|FD-Score:3.76|P-value:1.04E-4 ID:SGTC_2997|Compound:9067075|FD-Score:-5.29|P-value:1.89E-7 ID:SGTC_3138|Compound:9097216|FD-Score:3.18|P-value:8.48E-4 ID:SGTC_3141|Compound:9094609|FD-Score:-3.92|P-value:8.60E-5 ID:SGTC_3216|Compound:9129716|FD-Score:3.84|P-value:7.85E-5 ID:SGTC_3298|Compound:9116858|FD-Score:-3.27|P-value:9.02E-4 ID:SGTC_3330|Compound:9141424|FD-Score:3.33|P-value:4.96E-4

Top fitness defect scores for YNL119W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1844 rapamycin 14.50 4.0 nM 1.62E-45 15 9.18E-48
2 SGTC_2679 rapamycin 12.30 1.0 nM 1.34E-33 15 3.44E-35
3 SGTC_2358 9037157 6.08 4.0 µM 1.28E-9 6 6.10E-10
4 SGTC_2688 kanamycin b 5.92 96.7 µM 3.21E-9 calcium & mitochondrial duress 162 1.60E-9
5 SGTC_1899 st063254 4.90 59.6 µM 7.23E-7 2 4.69E-7
6 SGTC_567 1080-0542 4.68 68.4 µM 2.14E-6 3 1.45E-6
7 SGTC_941 3237-0776 4.35 460.0 µM 9.16E-6 7 6.66E-6
8 SGTC_1748 st037455 4.32 53.6 µM 1.08E-5 21 7.95E-6
9 SGTC_2127 5253358 4.11 187.9 µM 2.55E-5 23 1.94E-5
10 SGTC_511 blebbistatin 4.02 171.0 µM 3.79E-5 DNA intercalators 21 2.93E-5
11 SGTC_3216 9129716 3.84 49.5 µM 7.85E-5 RPP1 & pyrimidine depletion 27 6.27E-5
12 SGTC_2949 9072542 3.76 2.2 µM 1.04E-4 TSC3-RPN4 42 8.40E-5
13 SGTC_1038 k292-0204 3.73 154.0 µM 1.18E-4 24 9.59E-5
14 SGTC_1107 amlodipine 3.72 47.0 µM 1.22E-4 49 9.94E-5
15 SGTC_808 0711-0032 3.71 135.0 µM 1.25E-4 18 1.02E-4
16 SGTC_467 ro 31-8220 3.71 72.8 µM 1.28E-4 calcium & mitochondrial duress 97 1.05E-4
17 SGTC_2093 5331342 3.66 194.4 µM 1.55E-4 30 1.27E-4
18 SGTC_761 k216-0224 3.66 480.0 µM 1.56E-4 28 1.29E-4
19 SGTC_1208 2323-2347 3.64 50.3 µM 1.65E-4 12 1.36E-4
20 SGTC_2933 9026794 3.53 32.5 µM 2.44E-4 37 2.05E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.394 7.14E-125 YKL203C TOR2 het PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that regulates growth in response to nutrients and TORC2, a complex that regulates cell-cycle dependent polarization of the actin cytoskeleton; involved in meiosis; TOR2 has a paralog, TOR1, that arose from the whole genome duplication
0.359 1.12E-102 YLR114C AVL9 hom Conserved protein involved in exocytic transport from the Golgi; mutation is synthetically lethal with apl2 vps1 double mutation; member of a protein superfamily with orthologs in diverse organisms; relocalizes from bud neck to cytoplasm upon DNA replication stress
0.350 4.90E-97 YJR066W TOR1 hom PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that controls growth in response to nutrients by regulating translation, transcription, ribosome biogenesis, nutrient transport and autophagy; involved in meiosis; TOR1 has a paralog, TOR2, that arose from the whole genome duplication
0.322 1.95E-81 YML121W GTR1 hom Cytoplasmic GTP binding protein and negative regulator of the Ran/Tc4 GTPase cycle; component of GSE complex, which is required for sorting of Gap1p; involved in phosphate transport and telomeric silencing; similar to human RagA and RagB
0.320 1.35E-80 YKL110C KTI12 hom Protein that plays a role, with Elongator complex, in modification of wobble nucleosides in tRNA; involved in sensitivity to G1 arrest induced by zymocin; interacts with chromatin throughout the genome; also interacts with Cdc19p
0.320 1.68E-80 YGL211W NCS6 hom Protein required for thiolation of the uridine at the wobble position of Gln, Lys, and Glu tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae
0.316 2.47E-78 YHR187W IKI1 hom Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; iki1 mutations confer resistance to the K. lactis toxin zymocin
0.312 1.55E-76 YLR384C IKI3 hom Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; maintains structural integrity of Elongator; homolog of human IKAP, mutations in which cause familial dysautonomia (FD)
0.296 1.89E-68 YHR186C KOG1 het Subunit of TORC1, a rapamycin-sensitive complex involved in growth control that contains Tor1p or Tor2p, Lst8p and Tco89p; contains four HEAT repeats and seven WD-40 repeats; may act as a scaffold protein to couple TOR and its effectors
0.292 1.10E-66 YIL008W URM1 hom Ubiquitin-like protein involved in thiolation of cytoplasmic tRNAs; receives sulfur from the E1-like enzyme Uba4p and transfers it to tRNA; also functions as a protein tag with roles in nutrient sensing and oxidative stress response
0.280 1.94E-61 YGR200C ELP2 hom Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; target of Kluyveromyces lactis zymocin
0.279 6.47E-61 YHR111W UBA4 hom Protein that activates Urm1p before its conjugation to proteins (urmylation); also acts in thiolation of the wobble base of cytoplasmic tRNAs by adenylating and then thiolating Urm1p; receives sulfur from Tum1p
0.264 1.15E-54 YGR163W GTR2 hom Putative GTP binding protein that negatively regulates Ran/Tc4 GTPase cycle; activates transcription; subunit of EGO and GSE complexes; required for sorting of Gap1p; localizes to cytoplasm and to chromatin; homolog of human RagC and RagD
0.259 3.53E-52 YMR312W ELP6 hom Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity
0.256 6.11E-51 YDR158W HOM2 hom Aspartic beta semi-aldehyde dehydrogenase, catalyzes the second step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis