YNL142W / MEP2

Ammonium permease involved in regulation of pseudohyphal growth; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation

Zygosity: Homozygous strain
fixedexpanded
Profile for YNL142W / MEP2

Click on Significant Values for Screen Details ID:SGTC_28|Compound:pentachloropyridine N-oxide|FD-Score:-3.13|P-value:7.16E-4 ID:SGTC_32|Compound:0928-0142|FD-Score:-3.53|P-value:1.64E-4 ID:SGTC_127|Compound:0180-0333|FD-Score:-4.11|P-value:1.37E-5 ID:SGTC_128|Compound:0906-2981|FD-Score:-3.10|P-value:8.04E-4 ID:SGTC_129|Compound:0088-0017|FD-Score:-4.79|P-value:5.04E-7 ID:SGTC_163|Compound:1327-0036|FD-Score:3.76|P-value:5.35E-5 ID:SGTC_174|Compound:3553-0389|FD-Score:-3.43|P-value:2.40E-4 ID:SGTC_392|Compound:N-ethylmaleimide|FD-Score:-3.66|P-value:9.71E-5 ID:SGTC_395|Compound:anisomycin|FD-Score:-3.85|P-value:4.42E-5 ID:SGTC_524|Compound:r073-0010|FD-Score:-3.13|P-value:7.24E-4 ID:SGTC_610|Compound:4215-0184|FD-Score:3.89|P-value:3.04E-5 ID:SGTC_611|Compound:3453-1652|FD-Score:-5.03|P-value:1.45E-7 ID:SGTC_664|Compound:1222-0054|FD-Score:3.32|P-value:3.12E-4 ID:SGTC_746|Compound:1095-0025|FD-Score:3.47|P-value:1.75E-4 ID:SGTC_965|Compound:1291-5240|FD-Score:-3.37|P-value:3.02E-4 ID:SGTC_1028|Compound:k015-0029|FD-Score:3.03|P-value:8.87E-4 ID:SGTC_1033|Compound:k072-0102|FD-Score:-3.21|P-value:5.33E-4 ID:SGTC_1119|Compound:parthenolide|FD-Score:-4.17|P-value:1.05E-5 ID:SGTC_1130|Compound:3454-2858|FD-Score:-3.47|P-value:2.06E-4 ID:SGTC_1140|Compound:3229-1848|FD-Score:3.12|P-value:6.42E-4 ID:SGTC_1240|Compound:0356-0016|FD-Score:-4.26|P-value:7.07E-6 ID:SGTC_1251|Compound:0490-5699|FD-Score:6.36|P-value:3.16E-11 ID:SGTC_1258|Compound:0671-0043|FD-Score:-3.45|P-value:2.16E-4 ID:SGTC_1372|Compound:2752-0194|FD-Score:3.04|P-value:8.45E-4 ID:SGTC_1449|Compound:4112-4002|FD-Score:-3.97|P-value:2.57E-5 ID:SGTC_1630|Compound:st005209|FD-Score:3.23|P-value:4.42E-4 ID:SGTC_1696|Compound:st025591|FD-Score:3.51|P-value:1.48E-4 ID:SGTC_1771|Compound:st048882|FD-Score:-3.47|P-value:2.06E-4 ID:SGTC_1955|Compound:wogonin|FD-Score:-3.91|P-value:3.29E-5 ID:SGTC_2005|Compound:4024620|FD-Score:-3.08|P-value:8.67E-4 ID:SGTC_2050|Compound:5263056|FD-Score:3.45|P-value:1.91E-4 ID:SGTC_2130|Compound:NSC 7|FD-Score:3.66|P-value:8.09E-5 ID:SGTC_2306|Compound:7482397|FD-Score:-3.44|P-value:2.26E-4 ID:SGTC_2326|Compound:6164455|FD-Score:-4.18|P-value:1.02E-5 ID:SGTC_2690|Compound:usnic acid|FD-Score:-3.21|P-value:5.42E-4 ID:SGTC_2803|Compound:7707263|FD-Score:-3.06|P-value:9.24E-4 ID:SGTC_2804|Compound:7954240|FD-Score:4.72|P-value:5.98E-7 ID:SGTC_2810|Compound:7959871|FD-Score:7.29|P-value:3.37E-14 ID:SGTC_2813|Compound:7971799|FD-Score:3.29|P-value:3.50E-4 ID:SGTC_2848|Compound:9016370|FD-Score:3.62|P-value:9.62E-5 ID:SGTC_3301|Compound:9125354|FD-Score:-4.07|P-value:1.66E-5 ID:SGTC_28|Compound:pentachloropyridine N-oxide|FD-Score:-3.13|P-value:7.16E-4 ID:SGTC_32|Compound:0928-0142|FD-Score:-3.53|P-value:1.64E-4 ID:SGTC_127|Compound:0180-0333|FD-Score:-4.11|P-value:1.37E-5 ID:SGTC_128|Compound:0906-2981|FD-Score:-3.10|P-value:8.04E-4 ID:SGTC_129|Compound:0088-0017|FD-Score:-4.79|P-value:5.04E-7 ID:SGTC_163|Compound:1327-0036|FD-Score:3.76|P-value:5.35E-5 ID:SGTC_174|Compound:3553-0389|FD-Score:-3.43|P-value:2.40E-4 ID:SGTC_392|Compound:N-ethylmaleimide|FD-Score:-3.66|P-value:9.71E-5 ID:SGTC_395|Compound:anisomycin|FD-Score:-3.85|P-value:4.42E-5 ID:SGTC_524|Compound:r073-0010|FD-Score:-3.13|P-value:7.24E-4 ID:SGTC_610|Compound:4215-0184|FD-Score:3.89|P-value:3.04E-5 ID:SGTC_611|Compound:3453-1652|FD-Score:-5.03|P-value:1.45E-7 ID:SGTC_664|Compound:1222-0054|FD-Score:3.32|P-value:3.12E-4 ID:SGTC_746|Compound:1095-0025|FD-Score:3.47|P-value:1.75E-4 ID:SGTC_965|Compound:1291-5240|FD-Score:-3.37|P-value:3.02E-4 ID:SGTC_1028|Compound:k015-0029|FD-Score:3.03|P-value:8.87E-4 ID:SGTC_1033|Compound:k072-0102|FD-Score:-3.21|P-value:5.33E-4 ID:SGTC_1119|Compound:parthenolide|FD-Score:-4.17|P-value:1.05E-5 ID:SGTC_1130|Compound:3454-2858|FD-Score:-3.47|P-value:2.06E-4 ID:SGTC_1140|Compound:3229-1848|FD-Score:3.12|P-value:6.42E-4 ID:SGTC_1240|Compound:0356-0016|FD-Score:-4.26|P-value:7.07E-6 ID:SGTC_1251|Compound:0490-5699|FD-Score:6.36|P-value:3.16E-11 ID:SGTC_1258|Compound:0671-0043|FD-Score:-3.45|P-value:2.16E-4 ID:SGTC_1372|Compound:2752-0194|FD-Score:3.04|P-value:8.45E-4 ID:SGTC_1449|Compound:4112-4002|FD-Score:-3.97|P-value:2.57E-5 ID:SGTC_1630|Compound:st005209|FD-Score:3.23|P-value:4.42E-4 ID:SGTC_1696|Compound:st025591|FD-Score:3.51|P-value:1.48E-4 ID:SGTC_1771|Compound:st048882|FD-Score:-3.47|P-value:2.06E-4 ID:SGTC_1955|Compound:wogonin|FD-Score:-3.91|P-value:3.29E-5 ID:SGTC_2005|Compound:4024620|FD-Score:-3.08|P-value:8.67E-4 ID:SGTC_2050|Compound:5263056|FD-Score:3.45|P-value:1.91E-4 ID:SGTC_2130|Compound:NSC 7|FD-Score:3.66|P-value:8.09E-5 ID:SGTC_2306|Compound:7482397|FD-Score:-3.44|P-value:2.26E-4 ID:SGTC_2326|Compound:6164455|FD-Score:-4.18|P-value:1.02E-5 ID:SGTC_2690|Compound:usnic acid|FD-Score:-3.21|P-value:5.42E-4 ID:SGTC_2803|Compound:7707263|FD-Score:-3.06|P-value:9.24E-4 ID:SGTC_2804|Compound:7954240|FD-Score:4.72|P-value:5.98E-7 ID:SGTC_2810|Compound:7959871|FD-Score:7.29|P-value:3.37E-14 ID:SGTC_2813|Compound:7971799|FD-Score:3.29|P-value:3.50E-4 ID:SGTC_2848|Compound:9016370|FD-Score:3.62|P-value:9.62E-5 ID:SGTC_3301|Compound:9125354|FD-Score:-4.07|P-value:1.66E-5

Top fitness defect scores for YNL142W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2810 7959871 7.29 45.5 µM 3.37E-14 copper-dependent oxidative stress 7 1.52E-13
2 SGTC_1251 0490-5699 6.36 265.0 µM 3.16E-11 7 1.02E-10
3 SGTC_2804 7954240 4.72 19.5 µM 5.98E-7 17 1.18E-6
4 SGTC_610 4215-0184 3.89 155.0 µM 3.04E-5 exosome 14 4.94E-5
5 SGTC_163 1327-0036 3.76 7.5 µM 5.35E-5 116 8.45E-5
6 SGTC_2130 NSC 7 3.66 2.5 µM 8.09E-5 copper-dependent oxidative stress 37 1.25E-4
7 SGTC_2848 9016370 3.62 26.0 µM 9.62E-5 34 1.48E-4
8 SGTC_1696 st025591 3.51 34.8 µM 1.48E-4 SWF1 & branched chain AA biosynthesis 41 2.22E-4
9 SGTC_746 1095-0025 3.47 46.6 µM 1.75E-4 RSC & ERG11 24 2.61E-4
10 SGTC_2050 5263056 3.45 171.0 µM 1.91E-4 fatty acid desaturase (OLE1) 71 2.83E-4
11 SGTC_664 1222-0054 3.32 14.9 µM 3.12E-4 sphingolipid biosynthesis & PDR1 57 4.52E-4
12 SGTC_2813 7971799 3.29 71.4 µM 3.50E-4 46 5.03E-4
13 SGTC_1630 st005209 3.23 31.3 µM 4.42E-4 59 6.29E-4
14 SGTC_1140 3229-1848 3.12 3.0 µM 6.42E-4 29 8.96E-4
15 SGTC_1372 2752-0194 3.04 270.0 µM 8.45E-4 45 0.00116
16 SGTC_1028 k015-0029 3.03 4.8 µM 8.87E-4 64 0.00122
17 SGTC_522 1273-0010 2.98 11.6 µM 0.00106 60S ribosome export 58 0.00144
18 SGTC_1715 st032160 2.95 10.2 µM 0.00118 92 0.00160
19 SGTC_1849 st056239 2.92 19.2 µM 0.00128 RPP1 & pyrimidine depletion 78 0.00173
20 SGTC_2129 girgensonine 2.82 121.0 µM 0.00183 heme biosynthesis & mitochondrial translocase 101 0.00243

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.214 5.51E-36 YNL143C_p YNL143C_p hom Protein of unknown function; expressed at both mRNA and protein levels
0.135 5.00E-15 YOR005C DNL4 hom DNA ligase required for nonhomologous end-joining (NHEJ), forms stable heterodimer with required cofactor Lif1p, interacts with Nej1p; involved in meiosis, not essential for vegetative growth
0.124 4.81E-13 YDR210W_p YDR210W_p hom Predicted tail-anchored plasma membrane protein containing a conserved CYSTM module; related proteins in other organisms may be involved in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery
0.124 5.63E-13 YMR281W GPI12 het ER membrane protein involved in the second step of glycosylphosphatidylinositol (GPI) anchor assembly, the de-N-acetylation of the N-acetylglucosaminylphosphatidylinositol intermediate; functional homolog of human PIG-Lp
0.116 1.56E-11 YBR162W-A YSY6 hom Protein whose expression suppresses a secretory pathway mutation in E. coli; has similarity to the mammalian RAMP4 protein involved in secretion
0.107 5.56E-10 YDR502C SAM2 hom S-adenosylmethionine synthetase; catalyzes transfer of the adenosyl group of ATP to the sulfur atom of methionine; SAM2 has a paralog, SAM1, that arose from the whole genome duplication
0.098 1.25E-8 YEL001C IRC22 hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion localizes to the ER; YEL001C is non-essential; null mutant displays increased levels of spontaneous Rad52p foci
0.092 8.24E-8 YDR421W ARO80 hom Zinc finger transcriptional activator of the Zn2Cys6 family; activates transcription of aromatic amino acid catabolic genes in the presence of aromatic amino acids
0.091 1.39E-7 YNL262W POL2 het Catalytic subunit of DNA polymerase (II) epsilon, a chromosomal DNA replication polymerase that exhibits processivity and proofreading exonuclease activity; also involved in DNA synthesis during DNA repair; interacts extensively with Mrc1p
0.090 1.75E-7 YER052C HOM3 hom Aspartate kinase (L-aspartate 4-P-transferase); cytoplasmic enzyme that catalyzes the first step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis
0.090 1.98E-7 YEL071W DLD3 hom D-lactate dehydrogenase, part of the retrograde regulon which consists of genes whose expression is stimulated by damage to mitochondria and reduced in cells grown with glutamate as the sole nitrogen source, located in the cytoplasm
0.090 2.02E-7 YDR426C_d YDR426C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SNX41
0.089 2.17E-7 YFL044C OTU1 hom Deubiquitylation enzyme that binds to the chaperone-ATPase Cdc48p; may contribute to regulation of protein degradation by deubiquitylating substrates that have been ubiquitylated by Ufd2p; member of the Ovarian Tumor (OTU) family; protein abundance increases in response to DNA replication stress
0.089 2.19E-7 YOR060C SLD7 het Protein with a role in chromosomal DNA replication; interacts with Sld3p and reduces its affinity for Cdc45p; deletion mutant has aberrant mitochondria
0.087 4.44E-7 YGR193C PDX1 hom Dihydrolipoamide dehydrogenase (E3)-binding protein (E3BP) of the mitochondrial pyruvate dehydrogenase (PDH) complex, plays a structural role in the complex by binding and positioning E3 to the dihydrolipoamide acetyltransferase (E2) core