HIPHOP chemogenomics database

YNL142W / MEP2

Ammonium permease involved in regulation of pseudohyphal growth; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation

Zygosity: Homozygous strain

fixedexpanded
Profile for YNL142W / MEP2

Click on Significant Values for Screen Details

Top fitness defect scores for YNL142W deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_2810	7959871	7.29	45.5 µM	3.37E-14	copper-dependent oxidative stress	7	1.52E-13
2	SGTC_1251	0490-5699	6.36	265.0 µM	3.16E-11		7	1.02E-10
3	SGTC_2804	7954240	4.72	19.5 µM	5.98E-7		17	1.18E-6
4	SGTC_610	4215-0184	3.89	155.0 µM	3.04E-5	exosome	14	4.94E-5
5	SGTC_163	1327-0036	3.76	7.5 µM	5.35E-5		116	8.45E-5
6	SGTC_2130	NSC 7	3.66	2.5 µM	8.09E-5	copper-dependent oxidative stress	37	1.25E-4
7	SGTC_2848	9016370	3.62	26.0 µM	9.62E-5		34	1.48E-4
8	SGTC_1696	st025591	3.51	34.8 µM	1.48E-4	SWF1 & branched chain AA biosynthesis	41	2.22E-4
9	SGTC_746	1095-0025	3.47	46.6 µM	1.75E-4	RSC & ERG11	24	2.61E-4
10	SGTC_2050	5263056	3.45	171.0 µM	1.91E-4	fatty acid desaturase (OLE1)	71	2.83E-4
11	SGTC_664	1222-0054	3.32	14.9 µM	3.12E-4	sphingolipid biosynthesis & PDR1	57	4.52E-4
12	SGTC_2813	7971799	3.29	71.4 µM	3.50E-4		46	5.03E-4
13	SGTC_1630	st005209	3.23	31.3 µM	4.42E-4		59	6.29E-4
14	SGTC_1140	3229-1848	3.12	3.0 µM	6.42E-4		29	8.96E-4
15	SGTC_1372	2752-0194	3.04	270.0 µM	8.45E-4		45	0.00116
16	SGTC_1028	k015-0029	3.03	4.8 µM	8.87E-4		64	0.00122
17	SGTC_522	1273-0010	2.98	11.6 µM	0.00106	60S ribosome export	58	0.00144
18	SGTC_1715	st032160	2.95	10.2 µM	0.00118		92	0.00160
19	SGTC_1849	st056239	2.92	19.2 µM	0.00128	RPP1 & pyrimidine depletion	78	0.00173
20	SGTC_2129	girgensonine	2.82	121.0 µM	0.00183	heme biosynthesis & mitochondrial translocase	101	0.00243

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.214	5.51E-36	YNL143C_p	YNL143C_p	hom	Protein of unknown function; expressed at both mRNA and protein levels
0.135	5.00E-15	YOR005C	DNL4	hom	DNA ligase required for nonhomologous end-joining (NHEJ), forms stable heterodimer with required cofactor Lif1p, interacts with Nej1p; involved in meiosis, not essential for vegetative growth
0.124	4.81E-13	YDR210W_p	YDR210W_p	hom	Predicted tail-anchored plasma membrane protein containing a conserved CYSTM module; related proteins in other organisms may be involved in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery
0.124	5.63E-13	YMR281W	GPI12	het	ER membrane protein involved in the second step of glycosylphosphatidylinositol (GPI) anchor assembly, the de-N-acetylation of the N-acetylglucosaminylphosphatidylinositol intermediate; functional homolog of human PIG-Lp
0.116	1.56E-11	YBR162W-A	YSY6	hom	Protein whose expression suppresses a secretory pathway mutation in E. coli; has similarity to the mammalian RAMP4 protein involved in secretion
0.107	5.56E-10	YDR502C	SAM2	hom	S-adenosylmethionine synthetase; catalyzes transfer of the adenosyl group of ATP to the sulfur atom of methionine; SAM2 has a paralog, SAM1, that arose from the whole genome duplication
0.098	1.25E-8	YEL001C	IRC22	hom	Putative protein of unknown function; green fluorescent protein (GFP)-fusion localizes to the ER; YEL001C is non-essential; null mutant displays increased levels of spontaneous Rad52p foci
0.092	8.24E-8	YDR421W	ARO80	hom	Zinc finger transcriptional activator of the Zn2Cys6 family; activates transcription of aromatic amino acid catabolic genes in the presence of aromatic amino acids
0.091	1.39E-7	YNL262W	POL2	het	Catalytic subunit of DNA polymerase (II) epsilon, a chromosomal DNA replication polymerase that exhibits processivity and proofreading exonuclease activity; also involved in DNA synthesis during DNA repair; interacts extensively with Mrc1p
0.090	1.75E-7	YER052C	HOM3	hom	Aspartate kinase (L-aspartate 4-P-transferase); cytoplasmic enzyme that catalyzes the first step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis
0.090	1.98E-7	YEL071W	DLD3	hom	D-lactate dehydrogenase, part of the retrograde regulon which consists of genes whose expression is stimulated by damage to mitochondria and reduced in cells grown with glutamate as the sole nitrogen source, located in the cytoplasm
0.090	2.02E-7	YDR426C_d	YDR426C_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SNX41
0.089	2.17E-7	YFL044C	OTU1	hom	Deubiquitylation enzyme that binds to the chaperone-ATPase Cdc48p; may contribute to regulation of protein degradation by deubiquitylating substrates that have been ubiquitylated by Ufd2p; member of the Ovarian Tumor (OTU) family; protein abundance increases in response to DNA replication stress
0.089	2.19E-7	YOR060C	SLD7	het	Protein with a role in chromosomal DNA replication; interacts with Sld3p and reduces its affinity for Cdc45p; deletion mutant has aberrant mitochondria
0.087	4.44E-7	YGR193C	PDX1	hom	Dihydrolipoamide dehydrogenase (E3)-binding protein (E3BP) of the mitochondrial pyruvate dehydrogenase (PDH) complex, plays a structural role in the complex by binding and positioning E3 to the dihydrolipoamide acetyltransferase (E2) core