YNL145W / MFA2

Mating pheromone a-factor, made by a cells; interacts with alpha cells to induce cell cycle arrest and other responses leading to mating; biogenesis involves C-terminal modification, N-terminal proteolysis, and export; also encoded by MFA1

Zygosity: Homozygous strain
fixedexpanded
Profile for YNL145W / MFA2

Click on Significant Values for Screen Details ID:SGTC_38|Compound:0929-0051|FD-Score:2.85|P-value:7.72E-4 ID:SGTC_42|Compound:0133-0086|FD-Score:3.53|P-value:4.46E-5 ID:SGTC_91|Compound:0417-1751|FD-Score:-3.07|P-value:3.32E-4 ID:SGTC_150|Compound:3970-0969|FD-Score:2.94|P-value:5.53E-4 ID:SGTC_152|Compound:4469-0003|FD-Score:2.89|P-value:6.66E-4 ID:SGTC_417|Compound:st075190|FD-Score:3.01|P-value:4.21E-4 ID:SGTC_442|Compound:cape|FD-Score:2.83|P-value:8.26E-4 ID:SGTC_475|Compound:α-linolenic acid|FD-Score:-3.04|P-value:3.75E-4 ID:SGTC_505|Compound:gw-5074|FD-Score:2.84|P-value:8.19E-4 ID:SGTC_662|Compound:k832-1615|FD-Score:3.30|P-value:1.24E-4 ID:SGTC_701|Compound:4424-0219|FD-Score:4.62|P-value:1.42E-7 ID:SGTC_702|Compound:1488-0190|FD-Score:3.33|P-value:1.10E-4 ID:SGTC_737|Compound:1950-0117|FD-Score:2.99|P-value:4.50E-4 ID:SGTC_816|Compound:0325-0417|FD-Score:3.11|P-value:2.81E-4 ID:SGTC_894|Compound:0960-0238|FD-Score:3.22|P-value:1.74E-4 ID:SGTC_959|Compound:1222-0044|FD-Score:3.21|P-value:1.81E-4 ID:SGTC_965|Compound:1291-5240|FD-Score:3.29|P-value:1.29E-4 ID:SGTC_1043|Compound:0916-0009|FD-Score:2.88|P-value:6.95E-4 ID:SGTC_1132|Compound:3456-3614|FD-Score:3.29|P-value:1.29E-4 ID:SGTC_1255|Compound:0566-0045|FD-Score:8.66|P-value:3.47E-22 ID:SGTC_1276|Compound:0871-0068|FD-Score:2.79|P-value:9.64E-4 ID:SGTC_1407|Compound:3909-7909|FD-Score:2.88|P-value:6.90E-4 ID:SGTC_1513|Compound:0337-0559|FD-Score:-3.14|P-value:2.47E-4 ID:SGTC_1536|Compound:cytidine-2',3'-monophosphoric acid;  3'-cytidylic acid|FD-Score:-3.25|P-value:1.57E-4 ID:SGTC_1692|Compound:fluoroquinolonic acid|FD-Score:3.52|P-value:4.61E-5 ID:SGTC_1790|Compound:st045711|FD-Score:4.10|P-value:2.65E-6 ID:SGTC_1833|Compound:st055984|FD-Score:4.59|P-value:1.77E-7 ID:SGTC_1912|Compound:st060209|FD-Score:-3.31|P-value:1.19E-4 ID:SGTC_1916|Compound:st067606|FD-Score:-2.90|P-value:6.40E-4 ID:SGTC_1923|Compound:st058458|FD-Score:7.31|P-value:2.39E-16 ID:SGTC_1965|Compound:Pectolinarigenin|FD-Score:4.10|P-value:2.72E-6 ID:SGTC_1974|Compound:st069448|FD-Score:4.66|P-value:1.13E-7 ID:SGTC_1997|Compound:st073024|FD-Score:4.13|P-value:2.29E-6 ID:SGTC_2042|Compound:p-Dimethylaminoacetophenone|FD-Score:3.47|P-value:5.98E-5 ID:SGTC_2100|Compound:5377183|FD-Score:2.91|P-value:6.21E-4 ID:SGTC_2282|Compound:7944268|FD-Score:3.82|P-value:1.12E-5 ID:SGTC_2308|Compound:7584454|FD-Score:4.74|P-value:6.90E-8 ID:SGTC_2326|Compound:6164455|FD-Score:-2.81|P-value:8.98E-4 ID:SGTC_2397|Compound:5161689|FD-Score:2.96|P-value:5.09E-4 ID:SGTC_2408|Compound:sulbentine|FD-Score:-4.89|P-value:2.82E-8 ID:SGTC_2603|Compound:homopterocarpin|FD-Score:3.07|P-value:3.33E-4 ID:SGTC_2689|Compound:tacrolimus|FD-Score:2.97|P-value:4.91E-4 ID:SGTC_2786|Compound:7743617|FD-Score:-2.92|P-value:5.94E-4 ID:SGTC_2788|Compound:5282734|FD-Score:4.16|P-value:1.98E-6 ID:SGTC_2829|Compound:7992743|FD-Score:3.16|P-value:2.26E-4 ID:SGTC_2919|Compound:7978188|FD-Score:3.56|P-value:3.82E-5 ID:SGTC_2968|Compound:9090663|FD-Score:3.06|P-value:3.38E-4 ID:SGTC_2969|Compound:9091408|FD-Score:-3.59|P-value:3.40E-5 ID:SGTC_2977|Compound:9011197|FD-Score:3.00|P-value:4.38E-4 ID:SGTC_2996|Compound:9066192|FD-Score:3.14|P-value:2.49E-4 ID:SGTC_3129|Compound:9127257|FD-Score:3.38|P-value:8.64E-5 ID:SGTC_3148|Compound:9097893|FD-Score:5.03|P-value:1.19E-8 ID:SGTC_3292|Compound:9120825|FD-Score:3.77|P-value:1.40E-5 ID:SGTC_3301|Compound:9125354|FD-Score:5.80|P-value:5.92E-11 ID:SGTC_3310|Compound:9130229|FD-Score:4.18|P-value:1.77E-6 ID:SGTC_38|Compound:0929-0051|FD-Score:2.85|P-value:7.72E-4 ID:SGTC_42|Compound:0133-0086|FD-Score:3.53|P-value:4.46E-5 ID:SGTC_91|Compound:0417-1751|FD-Score:-3.07|P-value:3.32E-4 ID:SGTC_150|Compound:3970-0969|FD-Score:2.94|P-value:5.53E-4 ID:SGTC_152|Compound:4469-0003|FD-Score:2.89|P-value:6.66E-4 ID:SGTC_417|Compound:st075190|FD-Score:3.01|P-value:4.21E-4 ID:SGTC_442|Compound:cape|FD-Score:2.83|P-value:8.26E-4 ID:SGTC_475|Compound:α-linolenic acid|FD-Score:-3.04|P-value:3.75E-4 ID:SGTC_505|Compound:gw-5074|FD-Score:2.84|P-value:8.19E-4 ID:SGTC_662|Compound:k832-1615|FD-Score:3.30|P-value:1.24E-4 ID:SGTC_701|Compound:4424-0219|FD-Score:4.62|P-value:1.42E-7 ID:SGTC_702|Compound:1488-0190|FD-Score:3.33|P-value:1.10E-4 ID:SGTC_737|Compound:1950-0117|FD-Score:2.99|P-value:4.50E-4 ID:SGTC_816|Compound:0325-0417|FD-Score:3.11|P-value:2.81E-4 ID:SGTC_894|Compound:0960-0238|FD-Score:3.22|P-value:1.74E-4 ID:SGTC_959|Compound:1222-0044|FD-Score:3.21|P-value:1.81E-4 ID:SGTC_965|Compound:1291-5240|FD-Score:3.29|P-value:1.29E-4 ID:SGTC_1043|Compound:0916-0009|FD-Score:2.88|P-value:6.95E-4 ID:SGTC_1132|Compound:3456-3614|FD-Score:3.29|P-value:1.29E-4 ID:SGTC_1255|Compound:0566-0045|FD-Score:8.66|P-value:3.47E-22 ID:SGTC_1276|Compound:0871-0068|FD-Score:2.79|P-value:9.64E-4 ID:SGTC_1407|Compound:3909-7909|FD-Score:2.88|P-value:6.90E-4 ID:SGTC_1513|Compound:0337-0559|FD-Score:-3.14|P-value:2.47E-4 ID:SGTC_1536|Compound:cytidine-2',3'-monophosphoric acid;  3'-cytidylic acid|FD-Score:-3.25|P-value:1.57E-4 ID:SGTC_1692|Compound:fluoroquinolonic acid|FD-Score:3.52|P-value:4.61E-5 ID:SGTC_1790|Compound:st045711|FD-Score:4.10|P-value:2.65E-6 ID:SGTC_1833|Compound:st055984|FD-Score:4.59|P-value:1.77E-7 ID:SGTC_1912|Compound:st060209|FD-Score:-3.31|P-value:1.19E-4 ID:SGTC_1916|Compound:st067606|FD-Score:-2.90|P-value:6.40E-4 ID:SGTC_1923|Compound:st058458|FD-Score:7.31|P-value:2.39E-16 ID:SGTC_1965|Compound:Pectolinarigenin|FD-Score:4.10|P-value:2.72E-6 ID:SGTC_1974|Compound:st069448|FD-Score:4.66|P-value:1.13E-7 ID:SGTC_1997|Compound:st073024|FD-Score:4.13|P-value:2.29E-6 ID:SGTC_2042|Compound:p-Dimethylaminoacetophenone|FD-Score:3.47|P-value:5.98E-5 ID:SGTC_2100|Compound:5377183|FD-Score:2.91|P-value:6.21E-4 ID:SGTC_2282|Compound:7944268|FD-Score:3.82|P-value:1.12E-5 ID:SGTC_2308|Compound:7584454|FD-Score:4.74|P-value:6.90E-8 ID:SGTC_2326|Compound:6164455|FD-Score:-2.81|P-value:8.98E-4 ID:SGTC_2397|Compound:5161689|FD-Score:2.96|P-value:5.09E-4 ID:SGTC_2408|Compound:sulbentine|FD-Score:-4.89|P-value:2.82E-8 ID:SGTC_2603|Compound:homopterocarpin|FD-Score:3.07|P-value:3.33E-4 ID:SGTC_2689|Compound:tacrolimus|FD-Score:2.97|P-value:4.91E-4 ID:SGTC_2786|Compound:7743617|FD-Score:-2.92|P-value:5.94E-4 ID:SGTC_2788|Compound:5282734|FD-Score:4.16|P-value:1.98E-6 ID:SGTC_2829|Compound:7992743|FD-Score:3.16|P-value:2.26E-4 ID:SGTC_2919|Compound:7978188|FD-Score:3.56|P-value:3.82E-5 ID:SGTC_2968|Compound:9090663|FD-Score:3.06|P-value:3.38E-4 ID:SGTC_2969|Compound:9091408|FD-Score:-3.59|P-value:3.40E-5 ID:SGTC_2977|Compound:9011197|FD-Score:3.00|P-value:4.38E-4 ID:SGTC_2996|Compound:9066192|FD-Score:3.14|P-value:2.49E-4 ID:SGTC_3129|Compound:9127257|FD-Score:3.38|P-value:8.64E-5 ID:SGTC_3148|Compound:9097893|FD-Score:5.03|P-value:1.19E-8 ID:SGTC_3292|Compound:9120825|FD-Score:3.77|P-value:1.40E-5 ID:SGTC_3301|Compound:9125354|FD-Score:5.80|P-value:5.92E-11 ID:SGTC_3310|Compound:9130229|FD-Score:4.18|P-value:1.77E-6

Top fitness defect scores for YNL145W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1255 0566-0045 8.66 221.0 µM 3.47E-22 excess fatty acid 4 2.36E-18
2 SGTC_1923 st058458 7.31 19.1 µM 2.39E-16 4 1.32E-13
3 SGTC_3301 9125354 5.80 19.8 µM 5.92E-11 3 3.28E-9
4 SGTC_3148 9097893 5.03 49.5 µM 1.19E-8 22 2.48E-7
5 SGTC_2308 7584454 4.74 197.6 µM 6.90E-8 11 1.04E-6
6 SGTC_1974 st069448 4.66 26.9 µM 1.13E-7 15 1.56E-6
7 SGTC_701 4424-0219 4.62 185.0 µM 1.42E-7 calcium & mitochondrial duress 13 1.88E-6
8 SGTC_1833 st055984 4.59 71.0 µM 1.77E-7 8 2.25E-6
9 SGTC_3310 9130229 4.18 44.1 µM 1.77E-6 Golgi 20 1.48E-5
10 SGTC_2788 5282734 4.16 71.4 µM 1.98E-6 39 1.62E-5
11 SGTC_1997 st073024 4.13 93.8 µM 2.29E-6 7 1.83E-5
12 SGTC_1790 st045711 4.10 43.5 µM 2.65E-6 13 2.06E-5
13 SGTC_1965 Pectolinarigenin 4.10 58.4 µM 2.72E-6 RPP1 & pyrimidine depletion 13 2.10E-5
14 SGTC_2282 7944268 3.82 200.0 µM 1.12E-5 RPP1 & pyrimidine depletion 35 6.72E-5
15 SGTC_3292 9120825 3.77 59.8 µM 1.40E-5 14 8.04E-5
16 SGTC_2919 7978188 3.56 46.4 µM 3.82E-5 32 1.84E-4
17 SGTC_42 0133-0086 3.53 60.6 µM 4.46E-5 42 2.09E-4
18 SGTC_1692 fluoroquinolonic acid 3.52 71.0 µM 4.61E-5 12 2.14E-4
19 SGTC_2042 p-Dimethylaminoacetophenone 3.47 200.0 µM 5.98E-5 36 2.65E-4
20 SGTC_3129 9127257 3.38 49.5 µM 8.64E-5 41 3.58E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.165 6.15E-22 YDR209C_d YDR209C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized gene YDR210W.
0.160 1.65E-20 YDR479C PEX29 hom Peroxisomal integral membrane peroxin; involved in the regulation of peroxisomal size, number and distribution; genetic interactions suggest that Pex28p and Pex29p act at steps upstream of those mediated by Pex30p, Pex31p, and Pex32p; forms ER foci upon DNA replication stress
0.111 1.18E-10 YPR033C HTS1 het Cytoplasmic and mitochondrial histidine tRNA synthetase; efficient mitochondrial localization requires both a presequence and an amino-terminal sequence; mutations in human ortholog HARS2 are associated with Perrault syndrome
0.105 9.58E-10 YHR188C GPI16 het Transmembrane protein subunit of the glycosylphosphatidylinositol transamidase complex that adds GPIs to newly synthesized proteins; human PIG-Tp homolog
0.105 1.29E-9 YDR352W YPQ2 hom Putative vacuolar membrane transporter for cationic amino acids; likely contributes to amino acid homeostasis by exporting cationic amino acids from the vacuole; member of the PQ-loop family, with seven transmembrane domains; mutant phenotype is functionally complemented by rat PQLC2 vacuolar transporter
0.103 2.68E-9 YOR098C NUP1 het FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; possible karyopherin release factor that accelerates release of karyopherin-cargo complexes after transport across NPC; both NUP1 and NUP60 are homologous to human NUP153
0.094 5.22E-8 YKR077W MSA2 hom Putative transcriptional activator, that interacts with G1-specific transcription factor, MBF and G1-specific promoters; ortholog of Msa2p, an MBF and SBF activator that regulates G1-specific transcription and cell cycle initiation
0.094 5.83E-8 YKL031W_d YKL031W_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species
0.090 1.61E-7 YNR065C_p YNR065C_p hom Protein of unknown function; protein-protein interactions suggest a possible role in actin patch formation; YNR065C is not an essential gene
0.089 2.57E-7 YCL034W LSB5 hom Protein of unknown function; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization; may mediate disassembly of the Pan1 complex from the endocytic coat
0.087 4.46E-7 YCL044C MGR1 hom Subunit of the mitochondrial (mt) i-AAA protease supercomplex, which degrades misfolded mitochondrial proteins; forms a subcomplex with Mgr3p that binds to substrates to facilitate proteolysis; required for growth of cells lacking mtDNA
0.087 4.82E-7 YHR123W EPT1 hom sn-1,2-diacylglycerol ethanolamine- and cholinephosphotranferase; not essential for viability; EPT1 has a paralog, CPT1, that arose from the whole genome duplication
0.086 6.85E-7 YLL023C POM33 hom Transmembrane nucleoporin; involved in nuclear pore complex (NPC) distribution, assembly or stabilization; highly conserved across species, orthologous to human TMEM33 and paralogous to Per33p; protein abundance increases in response to DNA replication stress
0.085 8.21E-7 YJL087C TRL1 het tRNA ligase, required for tRNA splicing and for both splicing and translation of HAC1 mRNA in the UPR; has phosphodiesterase, polynucleotide kinase, and ligase activities; localized at the inner nuclear envelope and partially to polysomes
0.085 9.26E-7 YIL069C RPS24B hom Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S24, no bacterial homolog; RPS24B has a paralog, RPS24A, that arose from the whole genome duplication