YNL183C / NPR1

Protein kinase that stabilizes several plasma membrane amino acid transporters by antagonizing their ubiquitin-mediated degradation; phosphorylates Aly2p; negatively regulates Ldb19p-mediated endocytosis through phosphorylation of Ldb19p, which prevents its association with the plasma membrane; Npr1p activity is negatively regulated via phosphorylation by the TOR complex

Zygosity: Homozygous strain
fixedexpanded
Profile for YNL183C / NPR1

Click on Significant Values for Screen Details ID:SGTC_7|Compound:0192-1582|FD-Score:3.42|P-value:2.52E-4 ID:SGTC_46|Compound:0329-0187a|FD-Score:3.28|P-value:4.18E-4 ID:SGTC_127|Compound:0180-0333|FD-Score:4.24|P-value:8.25E-6 ID:SGTC_138|Compound:0850-0259|FD-Score:-4.66|P-value:2.00E-6 ID:SGTC_146|Compound:3448-8650|FD-Score:3.98|P-value:2.62E-5 ID:SGTC_172|Compound:1112-0046|FD-Score:4.77|P-value:6.63E-7 ID:SGTC_177|Compound:1327-0068|FD-Score:3.11|P-value:7.60E-4 ID:SGTC_178|Compound:4092-0806|FD-Score:4.14|P-value:1.32E-5 ID:SGTC_180|Compound:1486-1293|FD-Score:-3.16|P-value:9.38E-4 ID:SGTC_221|Compound:trifluoperazine|FD-Score:11.50|P-value:1.56E-31 ID:SGTC_367|Compound:0335-0881|FD-Score:3.03|P-value:9.83E-4 ID:SGTC_445|Compound:e6 berbamine|FD-Score:3.66|P-value:9.91E-5 ID:SGTC_449|Compound:xestospongin c|FD-Score:4.30|P-value:6.49E-6 ID:SGTC_513|Compound:4245-1575|FD-Score:-4.08|P-value:2.81E-5 ID:SGTC_522|Compound:1273-0010|FD-Score:3.94|P-value:3.19E-5 ID:SGTC_524|Compound:r073-0010|FD-Score:4.24|P-value:8.37E-6 ID:SGTC_531|Compound:3788-1495|FD-Score:-3.14|P-value:9.97E-4 ID:SGTC_543|Compound:3844-1095|FD-Score:-3.65|P-value:1.56E-4 ID:SGTC_547|Compound:1120-0019|FD-Score:4.93|P-value:2.88E-7 ID:SGTC_560|Compound:2332-1943|FD-Score:-3.30|P-value:5.74E-4 ID:SGTC_571|Compound:k831-0106|FD-Score:3.82|P-value:5.23E-5 ID:SGTC_582|Compound:0987-0078|FD-Score:-3.21|P-value:7.79E-4 ID:SGTC_737|Compound:1950-0117|FD-Score:-4.23|P-value:1.43E-5 ID:SGTC_751|Compound:0833-0568|FD-Score:4.22|P-value:9.25E-6 ID:SGTC_777|Compound:1315-0167|FD-Score:3.11|P-value:7.69E-4 ID:SGTC_780|Compound:0109-0199|FD-Score:3.22|P-value:5.26E-4 ID:SGTC_1263|Compound:0774-1208|FD-Score:3.28|P-value:4.22E-4 ID:SGTC_1345|Compound:1488-0192|FD-Score:4.48|P-value:2.73E-6 ID:SGTC_1424|Compound:4048-1941|FD-Score:3.21|P-value:5.44E-4 ID:SGTC_1451|Compound:4152-0210|FD-Score:3.63|P-value:1.09E-4 ID:SGTC_1524|Compound:corynanthine|FD-Score:4.97|P-value:2.40E-7 ID:SGTC_1591|Compound:genipin|FD-Score:-3.51|P-value:2.72E-4 ID:SGTC_1601|Compound:4'-hydroxyflavanone|FD-Score:-3.64|P-value:1.61E-4 ID:SGTC_1650|Compound:st011916|FD-Score:4.00|P-value:2.43E-5 ID:SGTC_1656|Compound:st012921|FD-Score:4.14|P-value:1.30E-5 ID:SGTC_1712|Compound:st032288|FD-Score:3.79|P-value:5.74E-5 ID:SGTC_1745|Compound:st081495|FD-Score:-3.41|P-value:3.92E-4 ID:SGTC_1747|Compound:st037496|FD-Score:3.19|P-value:5.70E-4 ID:SGTC_1750|Compound:st081859|FD-Score:3.54|P-value:1.60E-4 ID:SGTC_1754|Compound:st042065|FD-Score:10.50|P-value:1.21E-26 ID:SGTC_1760|Compound:st045453|FD-Score:8.97|P-value:7.53E-20 ID:SGTC_1800|Compound:st050079|FD-Score:3.99|P-value:2.55E-5 ID:SGTC_1801|Compound:st050089|FD-Score:5.98|P-value:7.52E-10 ID:SGTC_1803|Compound:st050068|FD-Score:8.12|P-value:1.33E-16 ID:SGTC_1818|Compound:st052136|FD-Score:4.08|P-value:1.70E-5 ID:SGTC_1844|Compound:rapamycin|FD-Score:-5.04|P-value:2.87E-7 ID:SGTC_1871|Compound:st057448|FD-Score:3.86|P-value:4.47E-5 ID:SGTC_1988|Compound:st071872|FD-Score:3.79|P-value:5.90E-5 ID:SGTC_2013|Compound:4024628|FD-Score:3.68|P-value:8.99E-5 ID:SGTC_2015|Compound:5119600|FD-Score:4.72|P-value:8.65E-7 ID:SGTC_2021|Compound:4019055|FD-Score:4.63|P-value:1.35E-6 ID:SGTC_2036|Compound:5160441|FD-Score:3.33|P-value:3.54E-4 ID:SGTC_2249|Compound:haloperidol|FD-Score:5.20|P-value:7.11E-8 ID:SGTC_2250|Compound:alverine citrate|FD-Score:6.18|P-value:2.04E-10 ID:SGTC_2253|Compound:7938677|FD-Score:-3.21|P-value:7.78E-4 ID:SGTC_2257|Compound:7960413|FD-Score:4.88|P-value:3.76E-7 ID:SGTC_2273|Compound:7815444|FD-Score:-3.23|P-value:7.45E-4 ID:SGTC_2434|Compound:5670830|FD-Score:3.54|P-value:1.55E-4 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:3.18|P-value:6.03E-4 ID:SGTC_2679|Compound:rapamycin|FD-Score:-4.14|P-value:2.15E-5 ID:SGTC_2688|Compound:kanamycin b|FD-Score:4.02|P-value:2.24E-5 ID:SGTC_2756|Compound:carbadox|FD-Score:3.53|P-value:1.66E-4 ID:SGTC_2831|Compound:7999036|FD-Score:5.25|P-value:5.45E-8 ID:SGTC_2896|Compound:9052656|FD-Score:5.23|P-value:6.01E-8 ID:SGTC_3037|Compound:9091026|FD-Score:3.46|P-value:2.16E-4 ID:SGTC_3101|Compound:9120531|FD-Score:3.80|P-value:5.62E-5 ID:SGTC_3152|Compound:9099253|FD-Score:5.77|P-value:2.64E-9 ID:SGTC_3181|Compound:9108277|FD-Score:3.26|P-value:4.53E-4 ID:SGTC_3186|Compound:9110464|FD-Score:3.04|P-value:9.70E-4 ID:SGTC_7|Compound:0192-1582|FD-Score:3.42|P-value:2.52E-4 ID:SGTC_46|Compound:0329-0187a|FD-Score:3.28|P-value:4.18E-4 ID:SGTC_127|Compound:0180-0333|FD-Score:4.24|P-value:8.25E-6 ID:SGTC_138|Compound:0850-0259|FD-Score:-4.66|P-value:2.00E-6 ID:SGTC_146|Compound:3448-8650|FD-Score:3.98|P-value:2.62E-5 ID:SGTC_172|Compound:1112-0046|FD-Score:4.77|P-value:6.63E-7 ID:SGTC_177|Compound:1327-0068|FD-Score:3.11|P-value:7.60E-4 ID:SGTC_178|Compound:4092-0806|FD-Score:4.14|P-value:1.32E-5 ID:SGTC_180|Compound:1486-1293|FD-Score:-3.16|P-value:9.38E-4 ID:SGTC_221|Compound:trifluoperazine|FD-Score:11.50|P-value:1.56E-31 ID:SGTC_367|Compound:0335-0881|FD-Score:3.03|P-value:9.83E-4 ID:SGTC_445|Compound:e6 berbamine|FD-Score:3.66|P-value:9.91E-5 ID:SGTC_449|Compound:xestospongin c|FD-Score:4.30|P-value:6.49E-6 ID:SGTC_513|Compound:4245-1575|FD-Score:-4.08|P-value:2.81E-5 ID:SGTC_522|Compound:1273-0010|FD-Score:3.94|P-value:3.19E-5 ID:SGTC_524|Compound:r073-0010|FD-Score:4.24|P-value:8.37E-6 ID:SGTC_531|Compound:3788-1495|FD-Score:-3.14|P-value:9.97E-4 ID:SGTC_543|Compound:3844-1095|FD-Score:-3.65|P-value:1.56E-4 ID:SGTC_547|Compound:1120-0019|FD-Score:4.93|P-value:2.88E-7 ID:SGTC_560|Compound:2332-1943|FD-Score:-3.30|P-value:5.74E-4 ID:SGTC_571|Compound:k831-0106|FD-Score:3.82|P-value:5.23E-5 ID:SGTC_582|Compound:0987-0078|FD-Score:-3.21|P-value:7.79E-4 ID:SGTC_737|Compound:1950-0117|FD-Score:-4.23|P-value:1.43E-5 ID:SGTC_751|Compound:0833-0568|FD-Score:4.22|P-value:9.25E-6 ID:SGTC_777|Compound:1315-0167|FD-Score:3.11|P-value:7.69E-4 ID:SGTC_780|Compound:0109-0199|FD-Score:3.22|P-value:5.26E-4 ID:SGTC_1263|Compound:0774-1208|FD-Score:3.28|P-value:4.22E-4 ID:SGTC_1345|Compound:1488-0192|FD-Score:4.48|P-value:2.73E-6 ID:SGTC_1424|Compound:4048-1941|FD-Score:3.21|P-value:5.44E-4 ID:SGTC_1451|Compound:4152-0210|FD-Score:3.63|P-value:1.09E-4 ID:SGTC_1524|Compound:corynanthine|FD-Score:4.97|P-value:2.40E-7 ID:SGTC_1591|Compound:genipin|FD-Score:-3.51|P-value:2.72E-4 ID:SGTC_1601|Compound:4'-hydroxyflavanone|FD-Score:-3.64|P-value:1.61E-4 ID:SGTC_1650|Compound:st011916|FD-Score:4.00|P-value:2.43E-5 ID:SGTC_1656|Compound:st012921|FD-Score:4.14|P-value:1.30E-5 ID:SGTC_1712|Compound:st032288|FD-Score:3.79|P-value:5.74E-5 ID:SGTC_1745|Compound:st081495|FD-Score:-3.41|P-value:3.92E-4 ID:SGTC_1747|Compound:st037496|FD-Score:3.19|P-value:5.70E-4 ID:SGTC_1750|Compound:st081859|FD-Score:3.54|P-value:1.60E-4 ID:SGTC_1754|Compound:st042065|FD-Score:10.50|P-value:1.21E-26 ID:SGTC_1760|Compound:st045453|FD-Score:8.97|P-value:7.53E-20 ID:SGTC_1800|Compound:st050079|FD-Score:3.99|P-value:2.55E-5 ID:SGTC_1801|Compound:st050089|FD-Score:5.98|P-value:7.52E-10 ID:SGTC_1803|Compound:st050068|FD-Score:8.12|P-value:1.33E-16 ID:SGTC_1818|Compound:st052136|FD-Score:4.08|P-value:1.70E-5 ID:SGTC_1844|Compound:rapamycin|FD-Score:-5.04|P-value:2.87E-7 ID:SGTC_1871|Compound:st057448|FD-Score:3.86|P-value:4.47E-5 ID:SGTC_1988|Compound:st071872|FD-Score:3.79|P-value:5.90E-5 ID:SGTC_2013|Compound:4024628|FD-Score:3.68|P-value:8.99E-5 ID:SGTC_2015|Compound:5119600|FD-Score:4.72|P-value:8.65E-7 ID:SGTC_2021|Compound:4019055|FD-Score:4.63|P-value:1.35E-6 ID:SGTC_2036|Compound:5160441|FD-Score:3.33|P-value:3.54E-4 ID:SGTC_2249|Compound:haloperidol|FD-Score:5.20|P-value:7.11E-8 ID:SGTC_2250|Compound:alverine citrate|FD-Score:6.18|P-value:2.04E-10 ID:SGTC_2253|Compound:7938677|FD-Score:-3.21|P-value:7.78E-4 ID:SGTC_2257|Compound:7960413|FD-Score:4.88|P-value:3.76E-7 ID:SGTC_2273|Compound:7815444|FD-Score:-3.23|P-value:7.45E-4 ID:SGTC_2434|Compound:5670830|FD-Score:3.54|P-value:1.55E-4 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:3.18|P-value:6.03E-4 ID:SGTC_2679|Compound:rapamycin|FD-Score:-4.14|P-value:2.15E-5 ID:SGTC_2688|Compound:kanamycin b|FD-Score:4.02|P-value:2.24E-5 ID:SGTC_2756|Compound:carbadox|FD-Score:3.53|P-value:1.66E-4 ID:SGTC_2831|Compound:7999036|FD-Score:5.25|P-value:5.45E-8 ID:SGTC_2896|Compound:9052656|FD-Score:5.23|P-value:6.01E-8 ID:SGTC_3037|Compound:9091026|FD-Score:3.46|P-value:2.16E-4 ID:SGTC_3101|Compound:9120531|FD-Score:3.80|P-value:5.62E-5 ID:SGTC_3152|Compound:9099253|FD-Score:5.77|P-value:2.64E-9 ID:SGTC_3181|Compound:9108277|FD-Score:3.26|P-value:4.53E-4 ID:SGTC_3186|Compound:9110464|FD-Score:3.04|P-value:9.70E-4

Top fitness defect scores for YNL183C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_221 trifluoperazine 11.50 10.3 µM 1.56E-31 NEO1-PIK1 41 3.88E-31
2 SGTC_1754 st042065 10.50 16.1 µM 1.21E-26 NEO1-PIK1 19 2.71E-26
3 SGTC_1760 st045453 8.97 10.4 µM 7.53E-20 NEO1-PIK1 41 1.46E-19
4 SGTC_1803 st050068 8.12 52.4 µM 1.33E-16 NEO1-PIK1 41 2.39E-16
5 SGTC_2250 alverine citrate 6.18 130.0 µM 2.04E-10 49 3.13E-10
6 SGTC_1801 st050089 5.98 28.4 µM 7.52E-10 NEO1-PIK1 49 1.13E-9
7 SGTC_3152 9099253 5.77 49.5 µM 2.64E-9 NEO1-PIK1 61 3.92E-9
8 SGTC_2831 7999036 5.25 19.5 µM 5.45E-8 16 7.78E-8
9 SGTC_2896 9052656 5.23 71.4 µM 6.01E-8 endomembrane recycling 51 8.57E-8
10 SGTC_2249 haloperidol 5.20 116.4 µM 7.11E-8 ergosterol depletion effects on membrane 59 1.01E-7
11 SGTC_1524 corynanthine 4.97 62.1 µM 2.40E-7 9 3.35E-7
12 SGTC_547 1120-0019 4.93 610.0 nM 2.88E-7 azole & statin 15 4.01E-7
13 SGTC_2257 7960413 4.88 200.0 µM 3.76E-7 NEO1-PIK1 29 5.23E-7
14 SGTC_172 1112-0046 4.77 2.6 µM 6.63E-7 NEO1 23 9.15E-7
15 SGTC_2015 5119600 4.72 184.0 µM 8.65E-7 18 1.19E-6
16 SGTC_2021 4019055 4.63 173.0 µM 1.35E-6 endomembrane recycling 82 1.85E-6
17 SGTC_1345 1488-0192 4.48 31.4 µM 2.73E-6 8 3.68E-6
18 SGTC_449 xestospongin c 4.30 11.2 µM 6.49E-6 NEO1-PIK1 53 8.65E-6
19 SGTC_127 0180-0333 4.24 59.1 µM 8.25E-6 mitochondrial response to ROS 32 1.10E-5
20 SGTC_524 r073-0010 4.24 27.8 µM 8.37E-6 45 1.11E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.486 4.53E-198 YML037C_p YML037C_p hom Putative protein of unknown function with some characteristics of a transcriptional activator; may be a target of Dbf2p-Mob1p kinase; GFP-fusion protein co-localizes with clathrin-coated vesicles; YML037C is not an essential gene
0.461 2.17E-175 YNL267W PIK1 het Phosphatidylinositol 4-kinase; catalyzes first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokinesis through the actin cytoskeleton
0.461 6.35E-176 YOR357C SNX3 hom Sorting nexin required to maintain late-Golgi resident enzymes in their proper location by recycling molecules from the prevacuolar compartment; contains a PX domain and sequence similarity to human Snx3p
0.458 2.41E-173 YPR091C NVJ2 hom Lipid-binding ER protein, enriched at nucleus-vacuolar junctions (NVJ); may be involved in sterol metabolism or signaling at the NVJ; contains a synaptotagmin-like-mitochondrial- lipid binding protein (SMP) domain; binds phosphatidylinositols and other lipids in a large-scale study; may interact with ribosomes, based on co-purification experiments
0.450 3.33E-167 YLR020C YEH2 hom Steryl ester hydrolase, catalyzes steryl ester hydrolysis at the plasma membrane; involved in sterol metabolism
0.426 5.37E-148 YHR108W GGA2 hom Protein that interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner to facilitate traffic through the late Golgi; binds phosphatidylinositol 4-phosphate, which plays a role in TGN localization; has homology to gamma-adaptin
0.423 3.89E-146 YOR115C TRS33 hom One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic
0.397 5.44E-127 YBR246W RRT2 hom Protein required for last step of diphthamide biosynthesis; deletion leads to resistance to sordarin and accumulation of diphthine; WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rtt10p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription
0.396 1.06E-126 YJL154C VPS35 hom Endosomal subunit of membrane-associated retromer complex required for retrograde transport; receptor that recognizes retrieval signals on cargo proteins, forms subcomplex with Vps26p and Vps29p that selects cargo proteins for retrieval; interacts with Ypt7p
0.377 1.47E-113 YBR245C ISW1 hom ATPase subunit of imitation-switch (ISWI) class chromatin remodelers; ATPase; forms a complex with Ioc3p (Isw1a), and a complex with Ioc2p and Ioc4p (Isw1b); Isw1a and Isw1b have partially overlapping and distinct roles, Isw1a involved in repression of transcription initiation and Isw1b involved in regulation of transcription elongation; Isw1b recruited to open reading frames by H3K36 methylation and acts with Chd1p to prevent trans-histone exchange over coding regions
0.364 2.00E-105 YGR166W TRS65 hom Subunit of TRAPPII, a multimeric guanine nucleotide-exchange factor for Ypt1p; involved in intra-Golgi traffic and the retrograde pathway from the endosome to Golgi; role in cell wall beta-glucan biosynthesis and the stress response
0.359 2.05E-102 YAR042W SWH1 hom Protein similar to mammalian oxysterol-binding protein; contains ankyrin repeats; localizes to the Golgi and the nucleus-vacuole junction
0.353 8.63E-99 YGL019W CKB1 hom Beta regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases
0.342 1.25E-92 YJL052W TDH1 hom Glyceraldehyde-3-phosphate dehydrogenase, isozyme 1; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; protein abundance increases in response to DNA replication stress
0.339 7.90E-91 YGL161C YIP5 hom Protein that interacts with Rab GTPases, localized to late Golgi vesicles; computational analysis of large-scale protein-protein interaction data suggests a possible role in vesicle-mediated transport