YNL191W / DUG3

Component of glutamine amidotransferase (GATase II); forms a complex with Dug2p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p)

Zygosity: Homozygous strain
fixedexpanded
Profile for YNL191W / DUG3

Click on Significant Values for Screen Details ID:SGTC_22|Compound:0964-0034|FD-Score:4.83|P-value:1.99E-7 ID:SGTC_106|Compound:0092-0003|FD-Score:3.57|P-value:8.89E-5 ID:SGTC_107|Compound:0358-0022|FD-Score:3.65|P-value:6.27E-5 ID:SGTC_132|Compound:0417-1715|FD-Score:-3.16|P-value:4.44E-4 ID:SGTC_198|Compound:3454-2873|FD-Score:3.16|P-value:4.48E-4 ID:SGTC_264|Compound:promethazine|FD-Score:3.20|P-value:3.81E-4 ID:SGTC_422|Compound:cantharidin|FD-Score:5.77|P-value:6.51E-10 ID:SGTC_426|Compound:0988-0037|FD-Score:3.01|P-value:7.91E-4 ID:SGTC_513|Compound:4245-1575|FD-Score:5.11|P-value:3.97E-8 ID:SGTC_587|Compound:k089-0003|FD-Score:-3.37|P-value:1.99E-4 ID:SGTC_729|Compound:4092-0585|FD-Score:3.21|P-value:3.67E-4 ID:SGTC_731|Compound:3966-0296|FD-Score:3.72|P-value:4.71E-5 ID:SGTC_841|Compound:0251-0215|FD-Score:-3.17|P-value:4.39E-4 ID:SGTC_849|Compound:0386-0493|FD-Score:3.65|P-value:6.38E-5 ID:SGTC_866|Compound:0495-0101|FD-Score:3.76|P-value:3.81E-5 ID:SGTC_869|Compound:0564-0080|FD-Score:-3.52|P-value:1.06E-4 ID:SGTC_872|Compound:0588-0139|FD-Score:-3.30|P-value:2.62E-4 ID:SGTC_926|Compound:2268-1401|FD-Score:3.21|P-value:3.76E-4 ID:SGTC_933|Compound:2914-0560|FD-Score:3.31|P-value:2.52E-4 ID:SGTC_936|Compound:3005-4620|FD-Score:-3.49|P-value:1.23E-4 ID:SGTC_971|Compound:1315-0384|FD-Score:3.42|P-value:1.65E-4 ID:SGTC_1033|Compound:k072-0102|FD-Score:3.72|P-value:4.72E-5 ID:SGTC_1035|Compound:k081-0041|FD-Score:3.49|P-value:1.23E-4 ID:SGTC_1161|Compound:k007-0610|FD-Score:2.97|P-value:9.06E-4 ID:SGTC_1264|Compound:0783-0040|FD-Score:3.54|P-value:9.90E-5 ID:SGTC_1567|Compound:st069303|FD-Score:3.08|P-value:6.07E-4 ID:SGTC_1589|Compound:2',5'-dimethoxyflavone|FD-Score:3.39|P-value:1.83E-4 ID:SGTC_1750|Compound:st081859|FD-Score:3.73|P-value:4.45E-5 ID:SGTC_1755|Compound:st041960|FD-Score:-4.21|P-value:4.95E-6 ID:SGTC_1758|Compound:st042942|FD-Score:3.96|P-value:1.56E-5 ID:SGTC_1759|Compound:st043059|FD-Score:5.28|P-value:1.48E-8 ID:SGTC_1792|Compound:st045773|FD-Score:3.08|P-value:6.12E-4 ID:SGTC_1859|Compound:st055998|FD-Score:3.70|P-value:5.14E-5 ID:SGTC_1974|Compound:st069448|FD-Score:4.07|P-value:9.41E-6 ID:SGTC_2132|Compound:5317594|FD-Score:-3.67|P-value:5.68E-5 ID:SGTC_2133|Compound:5320837|FD-Score:-4.11|P-value:7.80E-6 ID:SGTC_2148|Compound:5546263|FD-Score:3.07|P-value:6.40E-4 ID:SGTC_2185|Compound:6113692|FD-Score:2.95|P-value:9.73E-4 ID:SGTC_2209|Compound:5-(5-methyl-2-thenylidene)-Barbituric acid|FD-Score:3.71|P-value:4.77E-5 ID:SGTC_2288|Compound:7946289|FD-Score:3.64|P-value:6.57E-5 ID:SGTC_2289|Compound:7946404|FD-Score:5.46|P-value:4.70E-9 ID:SGTC_2345|Compound:9052315|FD-Score:3.49|P-value:1.22E-4 ID:SGTC_2371|Compound:9071574|FD-Score:-3.01|P-value:7.84E-4 ID:SGTC_2381|Compound:9035592|FD-Score:3.07|P-value:6.24E-4 ID:SGTC_2598|Compound:perillyl alcohol|FD-Score:4.02|P-value:1.19E-5 ID:SGTC_2646|Compound:retinol|FD-Score:4.60|P-value:6.64E-7 ID:SGTC_2764|Compound:methyldopa|FD-Score:3.16|P-value:4.55E-4 ID:SGTC_2766|Compound:oltipraz|FD-Score:3.21|P-value:3.73E-4 ID:SGTC_2828|Compound:7998861|FD-Score:3.21|P-value:3.79E-4 ID:SGTC_2856|Compound:9022925|FD-Score:3.23|P-value:3.42E-4 ID:SGTC_2914|Compound:7968218|FD-Score:3.44|P-value:1.50E-4 ID:SGTC_2933|Compound:9026794|FD-Score:4.00|P-value:1.29E-5 ID:SGTC_2968|Compound:9090663|FD-Score:3.65|P-value:6.36E-5 ID:SGTC_2974|Compound:9094440|FD-Score:3.11|P-value:5.33E-4 ID:SGTC_3020|Compound:9089140|FD-Score:-3.43|P-value:1.54E-4 ID:SGTC_22|Compound:0964-0034|FD-Score:4.83|P-value:1.99E-7 ID:SGTC_106|Compound:0092-0003|FD-Score:3.57|P-value:8.89E-5 ID:SGTC_107|Compound:0358-0022|FD-Score:3.65|P-value:6.27E-5 ID:SGTC_132|Compound:0417-1715|FD-Score:-3.16|P-value:4.44E-4 ID:SGTC_198|Compound:3454-2873|FD-Score:3.16|P-value:4.48E-4 ID:SGTC_264|Compound:promethazine|FD-Score:3.20|P-value:3.81E-4 ID:SGTC_422|Compound:cantharidin|FD-Score:5.77|P-value:6.51E-10 ID:SGTC_426|Compound:0988-0037|FD-Score:3.01|P-value:7.91E-4 ID:SGTC_513|Compound:4245-1575|FD-Score:5.11|P-value:3.97E-8 ID:SGTC_587|Compound:k089-0003|FD-Score:-3.37|P-value:1.99E-4 ID:SGTC_729|Compound:4092-0585|FD-Score:3.21|P-value:3.67E-4 ID:SGTC_731|Compound:3966-0296|FD-Score:3.72|P-value:4.71E-5 ID:SGTC_841|Compound:0251-0215|FD-Score:-3.17|P-value:4.39E-4 ID:SGTC_849|Compound:0386-0493|FD-Score:3.65|P-value:6.38E-5 ID:SGTC_866|Compound:0495-0101|FD-Score:3.76|P-value:3.81E-5 ID:SGTC_869|Compound:0564-0080|FD-Score:-3.52|P-value:1.06E-4 ID:SGTC_872|Compound:0588-0139|FD-Score:-3.30|P-value:2.62E-4 ID:SGTC_926|Compound:2268-1401|FD-Score:3.21|P-value:3.76E-4 ID:SGTC_933|Compound:2914-0560|FD-Score:3.31|P-value:2.52E-4 ID:SGTC_936|Compound:3005-4620|FD-Score:-3.49|P-value:1.23E-4 ID:SGTC_971|Compound:1315-0384|FD-Score:3.42|P-value:1.65E-4 ID:SGTC_1033|Compound:k072-0102|FD-Score:3.72|P-value:4.72E-5 ID:SGTC_1035|Compound:k081-0041|FD-Score:3.49|P-value:1.23E-4 ID:SGTC_1161|Compound:k007-0610|FD-Score:2.97|P-value:9.06E-4 ID:SGTC_1264|Compound:0783-0040|FD-Score:3.54|P-value:9.90E-5 ID:SGTC_1567|Compound:st069303|FD-Score:3.08|P-value:6.07E-4 ID:SGTC_1589|Compound:2',5'-dimethoxyflavone|FD-Score:3.39|P-value:1.83E-4 ID:SGTC_1750|Compound:st081859|FD-Score:3.73|P-value:4.45E-5 ID:SGTC_1755|Compound:st041960|FD-Score:-4.21|P-value:4.95E-6 ID:SGTC_1758|Compound:st042942|FD-Score:3.96|P-value:1.56E-5 ID:SGTC_1759|Compound:st043059|FD-Score:5.28|P-value:1.48E-8 ID:SGTC_1792|Compound:st045773|FD-Score:3.08|P-value:6.12E-4 ID:SGTC_1859|Compound:st055998|FD-Score:3.70|P-value:5.14E-5 ID:SGTC_1974|Compound:st069448|FD-Score:4.07|P-value:9.41E-6 ID:SGTC_2132|Compound:5317594|FD-Score:-3.67|P-value:5.68E-5 ID:SGTC_2133|Compound:5320837|FD-Score:-4.11|P-value:7.80E-6 ID:SGTC_2148|Compound:5546263|FD-Score:3.07|P-value:6.40E-4 ID:SGTC_2185|Compound:6113692|FD-Score:2.95|P-value:9.73E-4 ID:SGTC_2209|Compound:5-(5-methyl-2-thenylidene)-Barbituric acid|FD-Score:3.71|P-value:4.77E-5 ID:SGTC_2288|Compound:7946289|FD-Score:3.64|P-value:6.57E-5 ID:SGTC_2289|Compound:7946404|FD-Score:5.46|P-value:4.70E-9 ID:SGTC_2345|Compound:9052315|FD-Score:3.49|P-value:1.22E-4 ID:SGTC_2371|Compound:9071574|FD-Score:-3.01|P-value:7.84E-4 ID:SGTC_2381|Compound:9035592|FD-Score:3.07|P-value:6.24E-4 ID:SGTC_2598|Compound:perillyl alcohol|FD-Score:4.02|P-value:1.19E-5 ID:SGTC_2646|Compound:retinol|FD-Score:4.60|P-value:6.64E-7 ID:SGTC_2764|Compound:methyldopa|FD-Score:3.16|P-value:4.55E-4 ID:SGTC_2766|Compound:oltipraz|FD-Score:3.21|P-value:3.73E-4 ID:SGTC_2828|Compound:7998861|FD-Score:3.21|P-value:3.79E-4 ID:SGTC_2856|Compound:9022925|FD-Score:3.23|P-value:3.42E-4 ID:SGTC_2914|Compound:7968218|FD-Score:3.44|P-value:1.50E-4 ID:SGTC_2933|Compound:9026794|FD-Score:4.00|P-value:1.29E-5 ID:SGTC_2968|Compound:9090663|FD-Score:3.65|P-value:6.36E-5 ID:SGTC_2974|Compound:9094440|FD-Score:3.11|P-value:5.33E-4 ID:SGTC_3020|Compound:9089140|FD-Score:-3.43|P-value:1.54E-4

Top fitness defect scores for YNL191W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_422 cantharidin 5.77 155.0 µM 6.51E-10 cell wall signaling 22 3.86E-9
2 SGTC_2289 7946404 5.46 165.1 µM 4.70E-9 46 2.32E-8
3 SGTC_1759 st043059 5.28 70.0 µM 1.48E-8 amide catabolism 9 6.60E-8
4 SGTC_513 4245-1575 5.11 41.7 µM 3.97E-8 unfolded protein response 70 1.62E-7
5 SGTC_22 0964-0034 4.83 14.5 µM 1.99E-7 unfolded protein response 30 7.00E-7
6 SGTC_2646 retinol 4.60 96.8 µM 6.64E-7 2 2.09E-6
7 SGTC_1974 st069448 4.07 26.9 µM 9.41E-6 19 2.33E-5
8 SGTC_2598 perillyl alcohol 4.02 100.0 µM 1.19E-5 28 2.89E-5
9 SGTC_2933 9026794 4.00 32.5 µM 1.29E-5 17 3.12E-5
10 SGTC_1758 st042942 3.96 75.2 µM 1.56E-5 21 3.69E-5
11 SGTC_866 0495-0101 3.76 262.0 µM 3.81E-5 60S ribosome export 21 8.33E-5
12 SGTC_1750 st081859 3.73 2.4 µM 4.45E-5 endomembrane recycling 205 9.59E-5
13 SGTC_731 3966-0296 3.72 387.0 µM 4.71E-5 21 1.01E-4
14 SGTC_1033 k072-0102 3.72 21.3 µM 4.72E-5 60S ribosome export 27 1.01E-4
15 SGTC_2209 5-(5-methyl-2-thenylidene)-Barbituric acid 3.71 122.8 µM 4.77E-5 14 1.02E-4
16 SGTC_1859 st055998 3.70 20.0 µM 5.14E-5 35 1.09E-4
17 SGTC_107 0358-0022 3.65 2.7 µM 6.27E-5 SWF1 & branched chain AA biosynthesis 32 1.31E-4
18 SGTC_2968 9090663 3.65 33.4 µM 6.36E-5 44 1.33E-4
19 SGTC_849 0386-0493 3.65 241.0 µM 6.38E-5 27 1.33E-4
20 SGTC_2288 7946289 3.64 167.3 µM 6.57E-5 36 1.37E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.112 8.85E-11 YMR126C_p DLT1_p hom Protein of unknown function, mutant sensitive to 6-azauracil (6AU) and mycophenolic acid (MPA)
0.112 9.27E-11 YNL095C_p YNL095C_p hom Putative protein of unknown function; predicted to contain a transmembrane domain; not an essential gene; YNL095C has a paralog, ECM3, that arose from the whole genome duplication
0.111 1.21E-10 YJL061W NUP82 het Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup159p, and Nup116p)
0.107 5.57E-10 YFL031W HAC1 hom Basic leucine zipper (bZIP) transcription factor (ATF/CREB1 homolog); regulates the unfolded protein response, via UPRE binding, and membrane biogenesis; ER stress-induced splicing pathway facilitates efficient Hac1p synthesis; protein abundance increases in response to DNA replication stress
0.103 1.91E-9 YBR229C ROT2 hom Glucosidase II catalytic subunit required for normal cell wall synthesis; mutations in rot2 suppress tor2 mutations, and are synthetically lethal with rot1 mutations
0.095 3.24E-8 YHR061C GIC1 hom Protein involved in initiation of budding and cellular polarization; interacts with Cdc42p via the Cdc42/Rac-interactive binding (CRIB) domain; relocalizes from bud neck to nucleus upon DNA replication stress
0.095 3.88E-8 YDL117W CYK3 hom SH3-domain protein located in the bud neck and cytokinetic actin ring; relocalizes from bud neck to nucleus upon DNA replication stress; activates the chitin synthase activity of Chs2p during cytokinesis; suppressor of growth and cytokinesis defects of chs2 phospho-mutants
0.090 1.56E-7 YPR029C APL4 hom Gamma-adaptin, large subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in vesicle mediated transport
0.090 1.64E-7 YPR153W YPR153W hom Putative protein of unknown function
0.090 1.89E-7 YAL013W DEP1 hom Transcriptional modulator involved in regulation of structural phospholipid biosynthesis genes and metabolically unrelated genes, as well as maintenance of telomeres, mating efficiency, and sporulation
0.087 4.84E-7 YBR030W RKM3 hom Ribosomal lysine methyltransferase specific for monomethylation of Rpl42ap and Rpl42bp (lysine 40); nuclear SET domain containing protein
0.086 6.71E-7 YJR117W STE24 hom Highly conserved zinc metalloprotease that functions in two steps of a-factor maturation, C-terminal CAAX proteolysis and the first step of N-terminal proteolytic processing; contains multiple transmembrane spans
0.084 1.21E-6 YCL046W_d YCL046W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YCL045C
0.083 1.30E-6 YAR027W UIP3 hom Putative integral membrane protein of unknown function; interacts with Ulp1p at the nuclear periphery; member of DUP240 gene family
0.083 1.57E-6 YPL157W TGS1 hom Trimethyl guanosine synthase, conserved nucleolar methyl transferase that converts the m(7)G cap structure of snRNAs, snoRNAs, and telomerase TLC1 RNA to m(2,2,7)G; also required for nucleolar assembly and splicing of meiotic pre-mRNAs