YNL218W / MGS1

Protein with DNA-dependent ATPase and ssDNA annealing activities; involved in maintenance of genome; interacts functionally with DNA polymerase delta; homolog of human Werner helicase interacting protein (WHIP); forms nuclear foci upon DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YNL218W / MGS1

Click on Significant Values for Screen Details ID:SGTC_6|Compound:0844-0013|FD-Score:4.25|P-value:3.12E-7 ID:SGTC_179|Compound:1273-0063|FD-Score:-4.89|P-value:6.29E-9 ID:SGTC_248|Compound:streptozotocin|FD-Score:2.63|P-value:9.81E-4 ID:SGTC_252|Compound:miconazole|FD-Score:3.37|P-value:3.86E-5 ID:SGTC_264|Compound:promethazine|FD-Score:-3.12|P-value:1.43E-4 ID:SGTC_353|Compound:1988-1175|FD-Score:-4.01|P-value:1.59E-6 ID:SGTC_361|Compound:000s-0510|FD-Score:-2.76|P-value:6.76E-4 ID:SGTC_376|Compound:3970-0862|FD-Score:-4.00|P-value:1.65E-6 ID:SGTC_383|Compound:2324-0395|FD-Score:-2.93|P-value:3.27E-4 ID:SGTC_407|Compound:eburnamonine|FD-Score:-3.46|P-value:2.85E-5 ID:SGTC_448|Compound:monastrol|FD-Score:2.93|P-value:2.91E-4 ID:SGTC_656|Compound:4090-1979|FD-Score:4.06|P-value:9.33E-7 ID:SGTC_660|Compound:4031-1742|FD-Score:-3.15|P-value:1.27E-4 ID:SGTC_664|Compound:1222-0054|FD-Score:-3.01|P-value:2.34E-4 ID:SGTC_669|Compound:0108-0021|FD-Score:3.06|P-value:1.64E-4 ID:SGTC_701|Compound:4424-0219|FD-Score:2.80|P-value:4.98E-4 ID:SGTC_774|Compound:k060-0086|FD-Score:2.97|P-value:2.40E-4 ID:SGTC_936|Compound:3005-4620|FD-Score:2.66|P-value:8.64E-4 ID:SGTC_942|Compound:3253-1115|FD-Score:3.11|P-value:1.31E-4 ID:SGTC_1171|Compound:0368-0077|FD-Score:-2.74|P-value:7.40E-4 ID:SGTC_1462|Compound:k081-0014|FD-Score:-4.83|P-value:9.53E-9 ID:SGTC_1463|Compound:k081-0032|FD-Score:-5.52|P-value:6.61E-11 ID:SGTC_1829|Compound:berberine|FD-Score:4.05|P-value:1.00E-6 ID:SGTC_1835|Compound:st008666|FD-Score:-2.75|P-value:7.16E-4 ID:SGTC_1915|Compound:st067105|FD-Score:-3.70|P-value:8.32E-6 ID:SGTC_1917|Compound:st066507|FD-Score:-3.45|P-value:3.11E-5 ID:SGTC_2432|Compound:5630864|FD-Score:-3.00|P-value:2.42E-4 ID:SGTC_2438|Compound:5259590|FD-Score:2.76|P-value:5.95E-4 ID:SGTC_2492|Compound:derrusnin|FD-Score:2.69|P-value:7.86E-4 ID:SGTC_2494|Compound:18-aminoabieta-8,11,13-triene sulfate|FD-Score:4.25|P-value:3.09E-7 ID:SGTC_2611|Compound:pyromycin|FD-Score:-3.51|P-value:2.31E-5 ID:SGTC_2613|Compound:3',4'-dimethoxydalbergione|FD-Score:-2.82|P-value:5.39E-4 ID:SGTC_2614|Compound:arthonioic acid|FD-Score:-2.81|P-value:5.62E-4 ID:SGTC_2639|Compound:lactacystin|FD-Score:-3.06|P-value:1.91E-4 ID:SGTC_2842|Compound:9000675|FD-Score:2.69|P-value:7.76E-4 ID:SGTC_2844|Compound:9010103|FD-Score:2.89|P-value:3.34E-4 ID:SGTC_2884|Compound:9058502|FD-Score:-2.75|P-value:7.13E-4 ID:SGTC_2909|Compound:7941780|FD-Score:2.70|P-value:7.53E-4 ID:SGTC_2949|Compound:9072542|FD-Score:-3.06|P-value:1.89E-4 ID:SGTC_2957|Compound:9076800|FD-Score:2.68|P-value:7.98E-4 ID:SGTC_2979|Compound:9012552|FD-Score:-2.72|P-value:8.00E-4 ID:SGTC_3066|Compound:9117245|FD-Score:2.73|P-value:6.78E-4 ID:SGTC_3124|Compound:9127567|FD-Score:2.90|P-value:3.26E-4 ID:SGTC_6|Compound:0844-0013|FD-Score:4.25|P-value:3.12E-7 ID:SGTC_179|Compound:1273-0063|FD-Score:-4.89|P-value:6.29E-9 ID:SGTC_248|Compound:streptozotocin|FD-Score:2.63|P-value:9.81E-4 ID:SGTC_252|Compound:miconazole|FD-Score:3.37|P-value:3.86E-5 ID:SGTC_264|Compound:promethazine|FD-Score:-3.12|P-value:1.43E-4 ID:SGTC_353|Compound:1988-1175|FD-Score:-4.01|P-value:1.59E-6 ID:SGTC_361|Compound:000s-0510|FD-Score:-2.76|P-value:6.76E-4 ID:SGTC_376|Compound:3970-0862|FD-Score:-4.00|P-value:1.65E-6 ID:SGTC_383|Compound:2324-0395|FD-Score:-2.93|P-value:3.27E-4 ID:SGTC_407|Compound:eburnamonine|FD-Score:-3.46|P-value:2.85E-5 ID:SGTC_448|Compound:monastrol|FD-Score:2.93|P-value:2.91E-4 ID:SGTC_656|Compound:4090-1979|FD-Score:4.06|P-value:9.33E-7 ID:SGTC_660|Compound:4031-1742|FD-Score:-3.15|P-value:1.27E-4 ID:SGTC_664|Compound:1222-0054|FD-Score:-3.01|P-value:2.34E-4 ID:SGTC_669|Compound:0108-0021|FD-Score:3.06|P-value:1.64E-4 ID:SGTC_701|Compound:4424-0219|FD-Score:2.80|P-value:4.98E-4 ID:SGTC_774|Compound:k060-0086|FD-Score:2.97|P-value:2.40E-4 ID:SGTC_936|Compound:3005-4620|FD-Score:2.66|P-value:8.64E-4 ID:SGTC_942|Compound:3253-1115|FD-Score:3.11|P-value:1.31E-4 ID:SGTC_1171|Compound:0368-0077|FD-Score:-2.74|P-value:7.40E-4 ID:SGTC_1462|Compound:k081-0014|FD-Score:-4.83|P-value:9.53E-9 ID:SGTC_1463|Compound:k081-0032|FD-Score:-5.52|P-value:6.61E-11 ID:SGTC_1829|Compound:berberine|FD-Score:4.05|P-value:1.00E-6 ID:SGTC_1835|Compound:st008666|FD-Score:-2.75|P-value:7.16E-4 ID:SGTC_1915|Compound:st067105|FD-Score:-3.70|P-value:8.32E-6 ID:SGTC_1917|Compound:st066507|FD-Score:-3.45|P-value:3.11E-5 ID:SGTC_2432|Compound:5630864|FD-Score:-3.00|P-value:2.42E-4 ID:SGTC_2438|Compound:5259590|FD-Score:2.76|P-value:5.95E-4 ID:SGTC_2492|Compound:derrusnin|FD-Score:2.69|P-value:7.86E-4 ID:SGTC_2494|Compound:18-aminoabieta-8,11,13-triene sulfate|FD-Score:4.25|P-value:3.09E-7 ID:SGTC_2611|Compound:pyromycin|FD-Score:-3.51|P-value:2.31E-5 ID:SGTC_2613|Compound:3',4'-dimethoxydalbergione|FD-Score:-2.82|P-value:5.39E-4 ID:SGTC_2614|Compound:arthonioic acid|FD-Score:-2.81|P-value:5.62E-4 ID:SGTC_2639|Compound:lactacystin|FD-Score:-3.06|P-value:1.91E-4 ID:SGTC_2842|Compound:9000675|FD-Score:2.69|P-value:7.76E-4 ID:SGTC_2844|Compound:9010103|FD-Score:2.89|P-value:3.34E-4 ID:SGTC_2884|Compound:9058502|FD-Score:-2.75|P-value:7.13E-4 ID:SGTC_2909|Compound:7941780|FD-Score:2.70|P-value:7.53E-4 ID:SGTC_2949|Compound:9072542|FD-Score:-3.06|P-value:1.89E-4 ID:SGTC_2957|Compound:9076800|FD-Score:2.68|P-value:7.98E-4 ID:SGTC_2979|Compound:9012552|FD-Score:-2.72|P-value:8.00E-4 ID:SGTC_3066|Compound:9117245|FD-Score:2.73|P-value:6.78E-4 ID:SGTC_3124|Compound:9127567|FD-Score:2.90|P-value:3.26E-4

Top fitness defect scores for YNL218W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2494 18-aminoabieta-8,11,13-triene sulfate 4.25 3.7 µM 3.09E-7 66 1.07E-5
2 SGTC_6 0844-0013 4.25 19.8 µM 3.12E-7 68 1.08E-5
3 SGTC_656 4090-1979 4.06 187.0 µM 9.33E-7 14 2.42E-5
4 SGTC_1829 berberine 4.05 14.4 µM 1.00E-6 60S ribosome export 18 2.54E-5
5 SGTC_252 miconazole 3.37 1.3 nM 3.86E-5 ergosterol depletion effects on membrane 54 3.81E-4
6 SGTC_942 3253-1115 3.11 227.0 µM 1.31E-4 72 9.44E-4
7 SGTC_669 0108-0021 3.06 26.2 µM 1.64E-4 fatty acid desaturase (OLE1) 149 0.00112
8 SGTC_774 k060-0086 2.97 136.0 µM 2.40E-4 62 0.00148
9 SGTC_448 monastrol 2.93 171.0 µM 2.91E-4 36 0.00171
10 SGTC_3124 9127567 2.90 49.5 µM 3.26E-4 DNA damage response 56 0.00186
11 SGTC_2844 9010103 2.89 26.0 µM 3.34E-4 93 0.00190
12 SGTC_701 4424-0219 2.80 185.0 µM 4.98E-4 calcium & mitochondrial duress 73 0.00255
13 SGTC_2438 5259590 2.76 200.0 µM 5.95E-4 68 0.00292
14 SGTC_3066 9117245 2.73 49.5 µM 6.78E-4 75 0.00321
15 SGTC_2909 7941780 2.70 10.5 µM 7.53E-4 92 0.00348
16 SGTC_2842 9000675 2.69 19.5 µM 7.76E-4 94 0.00355
17 SGTC_2492 derrusnin 2.69 100.0 µM 7.86E-4 40 0.00359
18 SGTC_2957 9076800 2.68 11.5 µM 7.98E-4 82 0.00363
19 SGTC_936 3005-4620 2.66 401.0 µM 8.64E-4 115 0.00385
20 SGTC_248 streptozotocin 2.63 5.2 mM 9.81E-4 DNA damage response 99 0.00423

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.378 2.47E-114 YDR530C APA2 hom Diadenosine 5',5'''-P1,P4-tetraphosphate phosphorylase II; AP4A phosphorylase involved in catabolism of bis(5'-nucleosidyl) tetraphosphates; APA2 has a paralog, APA1, that arose from the whole genome duplication
0.305 3.00E-73 YDR521W_d YDR521W_d hom Dubious ORF that overlaps YDR520C; mutant increases expression of PIS1 and RPL3 in glycerol
0.304 6.97E-73 YJL171C YJL171C hom GPI-anchored cell wall protein of unknown function; induced in response to cell wall damaging agents and by mutations in genes involved in cell wall biogenesis; sequence similarity to YBR162C/TOS1, a covalently bound cell wall protein; protein abundance increases in response to DNA replication stress
0.298 5.34E-70 YDR169C STB3 hom Ribosomal RNA processing element (RRPE)-binding protein; involved in the glucose-induced transition from quiescence to growth; restricted to nucleus in quiescent cells, released into cytoplasm after glucose repletion; binds Sin3p; relative distribution to the nucleus increases upon DNA replication stress
0.298 7.83E-70 YGR022C_d YGR022C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF MTL1/YGR023W
0.295 1.97E-68 YMR074C YMR074C hom Protein with homology to human PDCD5; PDCD5 is involved in programmed cell death; N-terminal region forms a conserved triple-helix bundle structure; overexpression promotes H2O2-induced apoptosis; YMR074C is not an essential gene; protein abundance increases in response to DNA replication stress
0.289 3.49E-65 YNL166C BNI5 hom Protein involved in organization of septins at the mother-bud neck, may interact directly with the Cdc11p septin, localizes to bud neck in a septin-dependent manner
0.286 4.06E-64 YLL054C_p YLL054C_p hom Putative protein of unknown function with similarity to Pip2p, an oleate-specific transcriptional activator of peroxisome proliferation; YLL054C is not an essential gene
0.283 5.78E-63 YFL014W HSP12 hom Plasma membrane protein involved in maintaining membrane organization in stress conditions; induced by heat shock, oxidative stress, osmostress, stationary phase, glucose depletion, oleate and alcohol; protein abundance increased in response to DNA replication stress and dietary restriction; regulated by the HOG and Ras-Pka pathways; required for dietary restriction-induced lifespan extension
0.282 7.70E-62 YPR180W AOS1 het Subunit of a heterodimeric nuclear SUMO activating enzyme (E1) with Uba2p; activates Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability
0.281 4.55E-62 YGR011W_d YGR011W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.279 5.39E-61 YJR137C MET5 hom Sulfite reductase beta subunit, involved in amino acid biosynthesis, transcription repressed by methionine
0.278 1.59E-60 YBR274W CHK1 hom Serine/threonine kinase and DNA damage checkpoint effector, mediates cell cycle arrest via phosphorylation of Pds1p; phosphorylated by checkpoint signal transducer Mec1p; homolog of S. pombe and mammalian Chk1 checkpoint kinase
0.272 6.10E-58 YGR136W LSB1 hom Protein containing an N-terminal SH3 domain; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization; protein increases in abundance and forms nuclear foci in response to DNA replication stress
0.263 2.17E-54 YMR107W SPG4 hom Protein required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources